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嘌呤受体P2X_7激活谷氨酸受体NMDA引起视网膜神经节细胞凋亡

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中山大学学报（医学科学版） 2007年第5期28卷 490-494页

作者：张秀兰张梅胡慧玲 claire h.mitchell 葛坚眼科学国家重点实验室//中山大学中山眼科中心广东广州510060 厦门大学医学院眼科研究所厦门眼科中心福建厦门361005 宾夕法尼亚大学医学院生理学系美国费城19104

【目的】研究嘌呤受体P2X7和谷氨酸受体NMDA激活诱导大鼠视网膜神经节细胞(RGC)凋亡的相互作用机制。【方法】①对新生Long-Evan大鼠进行上丘注射荧光标记物Aminostilbamidine标记RGC,NMDA受体通道拮抗剂MK-801、APV和Memantine分别与P... 详细信息

【目的】研究嘌呤受体P2X7和谷氨酸受体NMDA激活诱导大鼠视网膜神经节细胞(RGC)凋亡的相互作用机制。【方法】①对新生Long-Evan大鼠进行上丘注射荧光标记物Aminostilbamidine标记RGC,NMDA受体通道拮抗剂MK-801、APV和Memantine分别与P2X7受体激动剂BzATP共培养,检测它们对体外培养RGC存活率的影响;②未经Aminostilbamidine标记的新生大鼠RGC,以10μmol/L钙离子(Ca2+)荧光染料Fura-2标记后,利用Ca2+影像测定仪分别测定BzATP及三种NMDA拮抗剂对RGC胞内Ca2+浓度的影响。【结果】(1)三种NMDA拮抗剂均分别不同程度阻断BzATP引起的RGC凋亡。BzATP在50μmol/L浓度下,约杀死(36%±2%)的RGC,而MK-801(10μmol/L)、APV(100μmol/L)和Memantine(100μmol/L)则均可明显减轻BzATP对RGC的毒性作用,使RGC存活率分别提高至(96%±4%,P<0.001)、(80%±5%,P=0.010)和(76%±9%,P=0.144)。(2)BzATP可引起RGC胞内Ca2+持续升高,在50μmol/L浓度下可使胞内Ca2+升高至(1183±109)nmol/L。而MK-801(10μmol/L)、APV(300μmol/L)和Memantine(30μmol/L)则均可显著降低BzATP介导的Ca2+升高幅度,分别为(76%±7%,P=0.003)、(51%±17%,P=0.033)和(55%±16%,P=0.025)。【结论】NMDA受体拮抗剂可阻断嘌呤受体P2X7激活诱导的RGC凋亡。P2X7受体和NMDA受体通道激活可能共同介导着兴奋性神经毒作用且P2X7在NMDA受体的上一环节先起作用。

关键词：视网膜神经节细胞 P2X7受体 NMDA

嘌呤受体P2X7激活谷氨酸受体NMDA引起视网膜神经节细胞凋亡

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嘌呤受体P2X7激活谷氨酸受体NMDA引起视网膜神经节细胞凋亡

溶酶体酸化可增强吞噬性星形胶质细胞的消化功能

中华医学会第十二届全国眼科学术大会

作者：张秀兰张梅胡慧玲 claire h.mitchell 葛坚中山大学中山眼科中心

目的：研究嘌呤受体 P2X7和谷氨酸受体 NMDA 激活诱导大鼠视网膜神经节细胞(RGCs)凋亡的相互作用机制。方法：(1)对新生 Long-Evan 大鼠进行上丘注射荧光标记物 Aminostilbamidine 标记 RGCs,NMDA 受体通道拮抗剂 MK

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来源： cnki会议评论

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神经损伤与功能重建 2015年第5期10卷 425-425页

作者： Kelly R.Monk M.Laura Feltri Carla Taveggia Camilla Lv claire h.mitchell Martin Simonsson Anna Erlandsson 唐颖馨 Department of Neuroscience Uppsala University Uppsala University Hospital Department of Anatomy and Cell Biology University of Pennsylvania Department of Physiology University of Pennsylvania SciL ife Lab and Department of Computer Science Electrical & Space Engineering Lulea University of Technology Department of Public health and Caring Sciences Uppsala University Rudbeck Laboratory

低效的溶酶体降解是多种脑部疾病发展的中心环节,但其具体机制及参与的细胞种类仍不清楚。既往研究显示,星形胶质细胞大量吞噬死亡的细胞,但将这些吞噬的物质储存在细胞内而不是进行降解。本研究对星形胶质细胞降解消化功能减弱的原因... 详细信息

低效的溶酶体降解是多种脑部疾病发展的中心环节,但其具体机制及参与的细胞种类仍不清楚。既往研究显示,星形胶质细胞大量吞噬死亡的细胞,但将这些吞噬的物质储存在细胞内而不是进行降解。本研究对星形胶质细胞降解消化功能减弱的原因进行研究,并探索增强星形胶质细胞降解消化功能的方法。结果显示,长期存在于吞噬泡周围的肌动环阻碍了溶酶体的吞噬溶解作用。此外,Rab27a蛋白可以通过Nox2减少溶酶体的酸化,而星形胶质细胞高表达Rab27a蛋白,阻碍抗原呈递。本研究还发现,Nox2与星形胶质细胞摄取的物质共定位,且表达主要组织相容性复合体II,提示这可能影响星形胶质细胞内的抗原呈递。使用酸化纳米粒子对星形胶质细胞溶酶体进行长时间的酸化处理,能增加星形胶质细胞对所摄取物质和死亡细胞的消化。但随着酸化时间的延长,星形胶质细胞的消化能力再次回复到较低水平,提示随着酸化作用的增强,细胞的对抗酸化的通路也相应增强了。

关键词： Nox2 消化星形胶质细胞溶酶体纳米粒子 phrodo 染料吞噬作用

Total dysphagia after short course of systemic corticotherapy:herpes simplex virus esophagitis

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World Journal of Gastroenterology 2013年第31期19卷 5178-5181页

作者： Isa Jetté-Cté Denise Ouellette claire Béliveau Andrew mitchell Division of Thoracic Surgery Maisonneuve-Rosemont Hospital University of MontrealMontreal H3L 2H8 Canada Department of Infectious Diseases and Microbiology Maisonneuve-Rosemont Hospital University of MontrealMontreal H3L 2H8 Canada Department of Pathology Maisonneuve-Rosemont Hospital University of Montreal Montreal H3L 2H8 Canada

A 72 year-old female developed a herpetic esophagitis after 3 d of oral corticotherapy for an acute exacerbation of chronic obstructive pulmonary disease,presenting as odynophagia and total *** were taken during a first esophagogastroduodenoscopy(EGD) and the patient was referred to the thoracic surgery service with a presumptive diagnosis of esophageal cancer.A second EGD was planned for dilatation,but by that time the stenosis was completely *** biopsies taken during the first EGD revealed multiple herpetic viral inclusions and ulcerations without any dysplasia or *** front of a severe esophageal stenosis,one must still exclude the usual differential diagnosis peptic stenosis and *** of endoscopic lesions can suggest the diagnosis but must be promptly confirmed by biopsy,viral culture or polymerase chain *** immune systemic effects of corticotherapy are well known and herpetic esophagitis occurs most frequently in immunocompromised individuals,this case emphasizes the importance of clinical awareness concerning short courses of corticotherapy for immunocompetent *** article discusses the reactivation process of herpetic infection in this context and addresses its diagnostic and therapeutic issues.

关键词： herpes simplex Esophagitis Dysphagia Corticosteroids Immunocompetence

Genetic and phenological variation of tocochromanol(vitamin E)content in wild(Daucus carota ***)and domesticated carrot(*** ***)

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horticulture Research 2014年第1期1卷 221-226页

作者： claire h Luby hiroshi A Maeda Irwin L Goldman Department of horticulture University of Wisconsin-MadisonMadisonWI 53706USA Department of Botany University of Wisconsin-MadisonMadisonWI 53706USA

Carrot roots(Daucus carota ***)produce tocochromanol compounds,collectively known as vitamin ***,little is known about their types and *** we determined the range and variation in types and amounts of tocochromanols in a variety of cultivated carrot accessions throughout carrot postharvest storage and reproductive stages and in wild-type roots(Daucus carota ***).Of eight possible tocochromanol compounds,we detected and quantified α-,and the combined peak for β-and γ-forms of tocopherols and *** variation in amounts of tocochromanol compounds was observed across accessions and over *** increases in a-tocopherol were noted during both reproductive growth and the postharvest *** variation of tocochromanols in carrot root tissue provides useful information for future research seeking to understand the role of these compounds in carrot root tissue or to breed varieties with increased levels of these compounds.

关键词： sativa cultivated vitamin

Circadian Clock Genes Universally Control Key Agricultural Traits

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Molecular Plant 2015年第8期8卷 1135-1152页

作者： claire Bendix Plant Gene Expression Center USDA-ARS Albany CA 94710 USA Department of Plant & Microbial Biology University of California Berkeley CA 94720 USA

Circadian clocks are endogenous timers that enable plants to synchronize biological processes with daily and seasonal environmental conditions in order to allocate resources during the most beneficial times of day and year. The circadian clock regulates a number of central plant activities, including growth, develop- ment, and reproduction, primarily through controlling a substantial proportion of transcriptional activity and protein function. This review examines the roles that alleles of circadian clock genes have played in domestication and improvement of crop plants. The focus here is on three groups of circadian clock genes essential to clock function in Arabidopsis thaliana： PSEUDO-RESPONSE REGULATORs, GIGANTEA, and the evening complex genes EARL Y FLOWERING 3, EARLY FLOWERING 4, and LUX ARRhYThMO. homol- ogous genes from each group underlie quantitative trait loci that have beneficial influences on key agricul- tural traits, especially flowering time but also yield, biomass, and biennial growth habit. Emerging insights into circadian clock regulation of other fundamental plant processes, including responses to abiotic and biotic stresses, are discussed to highlight promising avenues for further crop improvement.

关键词： circadian clock PSEUDO-RESPONSE REGULATORs GIGANTEA evening complex genes quantitative trait loci flowering time

hepatocellular carcinoma with portal invasion,still a place for liver surgery in the era of immunotherapy?

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hepatobiliary Surgery and Nutrition 2022年第5期11卷 782-784页

作者： héloïse Giudicelli claire Goumard Manon Allaire Service d’hépato-Gastroentérologie Hôpital Universitaire Pitié-SalpêtrièreAP-HP Sorbonne UniversitéParisFrance Service de Chirurgie hépatobiliopancréatique et Transplantation hépatique Hôpital Universitaire Pitié-SalpêtrièreAP-HP Sorbonne UniversitéParisFrance

hepatocellular carcinoma(hCC)is a leading cause of cancer death worldwide and is associated with a 5-year overall survival(OS)of 10-15%all stages ***,5-year OS reaches 50-70%when patients are in an early stage and can access curative treatments such as liver transplantation,liver resection(LR)or ***,LR is reserved to very selected patients with adequate performance status,preserved liver function,to be matched with grade of portal hypertension(PhT),sufficient volume of the future liver remnant and acceptable tumor burden(1,2).Worldwide,the definitions of“tumor burden”and vascular invasion vary as illustrated in Tables 1,2,and the impact of portal vein tumoral thrombosis(PVTT)on the choice for hCC treatment is not consensual(1,2).

关键词： treatment invasion matched

Quantifying Environmental Impacts of Temporary housing at the Urban Scale: Intersection of Vulnerability and Posthurricane Relief in New Orleans

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International Journal of Disaster Risk Science 2019年第4期10卷 478-492页

作者： claire McConnell Chiara Bertolin Department of Architecture and Technology Norwegian University of Science and Technology(NTNU)7491 TrondheimNorway Department of Mechanical and Industrial Engineering Norwegian University of Science and Technology(NTNU)7491 TrondheimNorway

The increasing risk and exposure of people and assets to natural hazards and disasters suggests an increasing need for temporary housing following *** to natural hazards is dependent on the resources available to families or communities to prepare for and mitigate risk,influenced by social *** study seeks to quantify the total environmental impact of temporary housing deployment in New Orleans,using the catastrophic impact of hurricane Katrina in southern Louisiana in August 2005 as a case *** employ a novel approach to estimate displacement period and take into account social vulnerability across New Orleans neighborhoods to better understand the scale of post-disaster relief and its global warming *** methodology implemented in this study comprises three steps:a risk assessment,a life cycle assessment,and a resulting total impact *** demonstrate the considerable risk of greenhouse gas emissions and energy impacts from temporary housing deployment linked to hurricane ***,we show that environmental impact is highly sensitive to displacement period and find the current methodology of anticipating temporary housing use by hazard alone to be ***,the approach presented in this article provides tools to politicians and disaster risk professionals that allow for resource investment planning to decrease social vulnerability,thus enhancing resilience and adaptive capacity in a more homogeneous way at the urban scale.

关键词： Disaster risk Global warming potential hurricane Katrina Life cycle assessment New Orleans Social vulnerability Temporary housing

Mechanisms and treatment strategies of demyelinating and dysmyelinating Charcot-Marie-Tooth disease

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Neural Regeneration Research 2023年第9期18卷 1931-1939页

作者： Nadège hertzog claire Jacob Faculty of Biology Institute of Developmental Biology and NeurobiologyJohannes Gutenberg University MainzHanns-Dieter-Hüsch-WegMainzGermany

Schwann cells,the myelinating glia of the peripheral nervous system,wrap axons multiple times to build their myelin *** is of paramount importance for axonal integrity and fast axon potential ***,myelin is lacking or dysfunctional in several neuropathies including demyelinating and dysmyelinating Charcot-M arie-To oth ***-Marie-To oth disease represents the most prevalent inherited neuropathy in humans and is classified either as axonal,demyelinating or dysmyelinating,or as *** demyelinating or dysmyelinating forms of Charcot-Marie-Tooth disease constitute the majority of the disease cases and are most frequently due to mutations in the three following myelin genes:peripheral myelin protein 22,myelin protein ze ro and gap junction beta 1(coding for Connexin 32) causing Charcot-M arie-Tooth disease type 1A,Charcot-Marie-Tooth disease type 1B,and X-linked Charcot-M arie-Tooth disease type 1,*** resulting perturbation of myelin structure and function leads to axonal demyelination or dysmyelination and causes severe disabilities in affected *** treatment to cure or slow down the disease progression is currently available on the market,howeve r,scientific discoveries led to a better understanding of the pathomechanisms of the disease and to potential treatment *** this review,we describe the features and molecular mechanisms of the three main demyelinating or dysmyelinating forms of Charcot-Marie-Tooth disease,the rodent models used in research,and the emerging therapeutic approaches to cure or counteract the progression of the disease.

关键词： Charcot-Marie-Tooth disease rodent models emerging treatments demyelination and dysmyelination endoplasmic reticulum stress gene therapy myelin repair Schwann cells unfolded protein response