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中国输血杂志 2001年第S1期14卷 277-280页

作者： Anthon Heyns Stephen Field chyang fang

A study of HIV-1 and HCV RNA on South African blood donors

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A study of HIV-1 and HCV RNA on South African blood donors

国际输血协会第十一届西太地区大会

作者： Anthon Heyns Stephen Field chyang fang National Blood Service Johannesburg South Africa Chiron Corporation Emeryville California USA.

South Africa(RSA) is in the grip of a devastating HIV/AIDS *** has had an effect on the safety of the blood *** blood transfusion services(BTSs)of the RSA have addressed the problem of the transmission of diseas- es by blood products by routinely screening all donations,856,375 in 2000,for the presence of HIV-1 p24 antigen,anti- bodies to HIV-1 and HIV-2,antibodies to HCV,HBsAg,and *** cornerstone of the management of the risk of transmitting particularly HIV,that is not detectable by the screening tests is a voluntary non-remunerated blood donor sys-

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论混合血清法筛检HIV抗体的可行性

中国输血杂志 1992年第1期5卷 5-8页

作者：季阳瞿德敏 chyang T. fang Ellen Lam Ba The Ha 中国医学科学院输血研究所美国红十字会国家传染病参比实验室

本实验论证了用10份血清标本混合检测HIV抗体的可行性。应用美国Organon Teknika公司的ELISA法HIV-1抗体检测试剂进行试验,在只变动血清标本量与标本稀释液量比例,但两者总量不变的情况下进行测定。10份血清混合后血清量由5μl扩大到50... 详细信息

本实验论证了用10份血清标本混合检测HIV抗体的可行性。应用美国Organon Teknika公司的ELISA法HIV-1抗体检测试剂进行试验,在只变动血清标本量与标本稀释液量比例,但两者总量不变的情况下进行测定。10份血清混合后血清量由5μl扩大到50μl,而标本稀释液量相应减少后标本的光密度(吸光值)略为增加,但均低于界限值,说明试验的特异性尚未受到影响。此外,将已经确证为HIV抗体阳性的血清标本1份同9份正常献血者血清混合后,经多次配对试验,均能正确反映出含有HIV抗体的标本,说明试验的敏感性不受影响,能保持原法的水平。由于混合血清法检测HIV抗体可节约开支10倍,同时节省人力、物力和工时等众多优点,故容易推广应用。

关键词：献血员艾滋病毒抗体混合血清法

Association between Helicobacter pylori seropositivity and digestive tract cancers

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World Journal of Gastroenterology 2009年第43期15卷 5465-5471页

作者： I-Chen Wu Deng-chyang Wu fang-Jung Yu Jaw-Yuan Wang Chao-Hung Kuo Sheau-fang Yang Chao-Ling wang Ming-Tsang Wu Division of Gastroenterology Department of Internal Medicine Kaohsiung Medical University Hospital Kaohsitmg 807 Taiwan China Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 807 Taiwan China Department of Pathology Kaohsiung Medical University Hospital Kaohsiung 807 Taiwan China Graduate Institute of Occupational Safety and Health Kaohsiung Medical University Kaohsiung 807 Taiwan China

AIM:To explore the role of Helicobacter pylori(H pylori) infection on the risk of digestive tract cancers. METHODS:In total,199 oral squamous-cell carcinoma(SCC),317 esophageal SCC,196 gastric cardia and non-cardia adenocarcinoma and 240 colon adenocarcinoma patients were recruited for serum tests of H pylori *** hospital-and one community-based control groups were used for the comparisons.H pyloriseropositivity was determined by an enzyme linked immunosorbent assay method against H pylori IgG. RESULTS:Presence of H pylori infection was significantly inversely associated with esophageal SCC[adjusted odds ratio(AOR):0.315-0.472,all P-value< 0.05]but positively associated with gastric adenocarcinoma(both cardia and non-cardia)(AOR:1.636-3.060, all P-value<0.05)in comparison to the three control *** results were not found in cancers of the oral cavity and colon. CONCLUSION:Our findings support the finding that H pylori seropositivity is inversely associated with esophageal SCC risk,but increases the risk of gastric cardia adenocarcinoma.

关键词：幽门螺杆菌阳性反应消化道癌症血清酶联免疫吸附试验幽门螺旋杆菌协会

Short-term Celecoxib intervention is a safe and effective chemopreventive for gastric carcinogenesis based on a Mongolian gerbil model

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World Journal of Gastroenterology 2009年第39期15卷 4907-4914页

作者： Chao-Hung Kuo Huang-Ming Hu Pei-Yun Tsai I-Chen Wu Sheau-fang Yang Lin-Li Chang Jaw-Yuan Wang Chang-Ming Jan Wen-Ming Wang Deng-chyang Wu Division of Internal Medicine Kaohsiung Municipal Hsiao-Kang Hospital Kaohsiung 813 Taiwan China Division of Gastroenterology Department of Internal Medicine Kaohsiung Medical University Hospital Kaohsiung 807 Taiwan China Department of Medicine Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 807 Taiwan China Department of Nursing Kaohsiung Municipal Hsiao-Kang Hospital Kaohsiung 813 Taiwan China Department of Pathology Kaohsiung Medical University Hospital Kaohsiung 807 Taiwan China Department of Microbiology Kaohsiung Medical University Kaohsiung 807 Taiwan China Department of Surgery Kaohsiung Medical University Hosoital Kaohsiung 807 Taiwan China

AIM:To evaluate the optimal intervention point of a selective cyclooxygenase-2(COX-2) inhibitor,Celecoxib,for inhibiting Helicobacter pylori(H pylori)-associated gastric carcinogenesis in Mongolian gerbils(MGs).METHODS:One hundred and twelve MGs were divided into six groups(A-F).One hundred gerbils were inoculated with H pylori(groups A-E).Twelve gerbils were inoculated with vehicle broth only(group F).After 4 wk,they were given N'-methyl-N' -nitro-N-nitroso-guanidine(MNNG)(50 μg/mL) in the drinking water for 20 *** groups B-E,the animals were given the stock Celecoxib(10 mg/kg per day) diet from the 21st,31st,21st and 41st week *** periods of administering Celecoxib were 30,20,20,and 15 wk *** the 51st week,the animals were sacrificed for histological *** PCNA expression was examined by the immunohistochemistry *** expression of COX-2 protein was assessed by Western *** used the χ2 *** difference was regarded as signifi cant when P value was less than ***:Seventeen percent(17/100) of H pylori-infected MGs developed gastric *** of these lesions were well-differentiated *** incidence rates of adenocarcinoma in groups A-F were 40%,0%,0%,20%,25%,and 0% *** inflammatory scores were higher in group B than in other *** was no inflammatory response noted in group *** treatment resulted in a significant reduction in the proliferation of H pylori-infected mucosal cells(groups B,C and D)(P < 0.01).The expression of COX-2 protein was significantly attenuated in the groups which were Celecoxib-treated for more than 20 wk(groups B,C,D).The groups treated with Celecoxib had a significantly lower rate of advanced gastric cancer(34% vs 75%,P<0.001) There were no sudden deaths in any of the ***:Short-term treatment with Celecoxib has an anti-carcinogenic effect,and resulted in less severe inflammation and inhibited the invasive degree of gastric c

关键词：抑制剂致癌作用肠癌治疗

First-week clinical responses to dexlansoprazole 60 mg and esomeprazole 40 mg for the treatment of grades A and B gastroesophageal reflux disease

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World Journal of Gastroenterology 2017年第47期23卷 8395-8404页

作者： Chih-Ming Liang Ming-Te Kuo Pin-I Hsu Chao-Hung Kuo Wei-Chen Tai Shih-Cheng Yang Keng-Liang Wu Hsing-Ming Wang Chih-Chien Yao Cheng-En Tsai Yao-Kuang Wang Jiunn-Wei Wang Chih-fang Huang Deng-chyang Wu Seng-Kee Chuah 无 Division of Hepato-Gastroenterology Department of Internal MedicineKaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine Division of Gastroenterology Department of Internal MedicineKaohsiung Veterans General HospitalNational Yang-Ming University Division of Gastroenterology Department of Internal MedicineKaohsiung Medical University Hospital and Kaohsiung Medical University Division of Family Physicians Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine Taiwan Acid-Related Disease Study Group

AIM To compare the one-week clinical effects of single doses of dexlansoprazole and esomeprazole on grades A and B erosive *** We enrolled 175 adult patients with gastroesophageal reflux disease(GERD). The patients were randomized in a 1:1 ratio into two sequence groups to define the order in which they received single doses of dexlansoprazole(n = 88) and esomeprazole(n = 87) for an intention-to-treat analysis. The primary endpoints were the complete symptom resolution(CSR) rates at days 1, 3, and 7 after drug *** Thirteen patients were lost to follow-up, resulting in 81 patients in each group for the per-protocol analysis. The CSRs for both groups were similar at days 1, 3 and 7. In the subgroup analysis, the female patients achieved higher CSRs in the dexlansoprazole group than in the esomeprazole group at day 3(38.3% vs 18.4%, P = 0.046). An increasing trend toward a higher CSR was observed in the dexlansoprazole group at day 7(55.3% vs 36.8%, P = 0.09). In the esomeprazole group, female sex was a negative predictive factor for CSR on post-administration day 1 [OR =-1.249 ± 0.543; 95%CI: 0.287(0.099-0.832), P = 0.022] and day 3 [OR =-1.254 ± 0.519; 95%CI: 0.285(0.103-0.789), P = 0.016]. Patients with spicy food eating habits achieved lower CSRs on day 1 [37.3% vs 21.4%, OR =-0.969 ± 0.438; 95%CI: 0.380(0.161-0.896), P = 0.027]. CONCLUSION The overall CSR for GERD patients was similar at days 1-7 for both the dexlansoprazole and esomeprazole groups, although a higher incidence of CSR was observed on day 3 in female patients who received a single dose of dexlansoprazole.

关键词： Dexlansoprazole Esomeprazole Oneweek response Complete symptom resolution rate Gastroesophageal reflux disease

黑麂和费氏麂四种卫星DNA的克隆特征和染色体定位(英文)

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动物学研究（英文） 2008年第3期29卷 225-235页

作者：刘妍佴文惠黄玲王金焕苏伟婷 LIN Chyi chyang 杨凤堂中国科学院昆明动物研究所遗传资源与进化重点实验室 Department of Medical Research China Medical University Hospital

近年来,分子细胞遗传学研究已基本证实了染色体的串联融合(端粒-着丝粒融合)是麂属动物核型演化的主要重排方式。尽管染色体串联融合的分子机制还不清楚,但通过染色体的非同源重组,着丝粒区域的卫星DNA被认为可能介导了染色体的融合。... 详细信息

近年来,分子细胞遗传学研究已基本证实了染色体的串联融合(端粒-着丝粒融合)是麂属动物核型演化的主要重排方式。尽管染色体串联融合的分子机制还不清楚,但通过染色体的非同源重组,着丝粒区域的卫星DNA被认为可能介导了染色体的融合。以前的研究发现在赤麂和小麂染色体的大部分假定的串联融合位点处存在着非随机分布的卫星DNA。然而在麂属的其他物种中,这些卫星DNA的组成以及在基因组中的分布情况尚未被研究。本研究从黑麂和费氏麂基因组中成功地克隆了4种卫星DNA(BMC5、BM700、BM1.1k和FM700),并分析了这些卫星克隆的特征以及在小麂、黑麂、贡山麂和费氏麂染色体上的定位情况。结果表明,卫星I和IIDNA(BMC5,BM700和FM700)的信号除了分布在这些麂属动物染色体的着丝粒区域外,也间隔地分布在这些物种的染色体臂上。其研究结果为黑麂、费氏麂和贡山麂的染色体核型也是从一个2n=70的共同祖先核型通过一系列的串联融合进化而来的假说提供了直接的证据。

关键词：荧光原位杂交定位卫星DNA 麂属染色体串联融合

Evaluation of urine ELISA test for detecting Helicobacter pylori infection in Taiwan: A prospective study

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World Journal of Gastroenterology 2005年第35期11卷 5545-5548页

作者： Fu-Chen Kuo Sheng-Wen Wang I-Chen Wu fang-Jung Yu Yuan-Chieh Yang Jeng-Yih Wu Wen-Ming Wang Deng-chyang Wu Department of Gynecology and Obstetrics E-DaHospital I-Shou University Kaohsiung Taiwan China Division of Gastroenterology Department of Internal Medicine Kaohsiung Medical University Hospital Taiwan China Department of Clinical Pathology KaohsiungMedical University Hospital Taiwan China

AIM: To evaluate the diagnostic accuracy and clinical utility of a new ELISA (URINELISA) test for detecting Helicobacter pylori(H pylori) antibody in the urine of Taiwan Residents ***: In this prospective study, 317 consecutive dyspeptic patients (171 men, 146 women; mean age,51.0 years) were included. They underwent gastroendoscopy for evaluation. Invasive tests, including culture, histology,and rapid urease test (RUT), and non-invasive 13C-urea breath test were preformed. At the same time, urine specimens were collected for URINELISA. The status of H pylori infection was considered as positive when either culture was positive, or when two of the other, RUT,histology or 13C-UBT, were ***: The sensitivity, specificity, positive predictive value, and negative predictive value of URINELISA are 91.7% (211/230), 90.8% (79/87), 96.3% (211/219), and 80.6% (79/98) ***: This URINELISA test is reliable, inexpensive and easy-to-use. The high diagnostic accuracy warrants the use of URINELISA as a first-line screening tool for diagnosis of H pylori infection in untreated patients.

关键词：尿液检查疾病传染台湾省中国幽门螺杆菌消化系统

Relationship between genetic polymorphisms of alcohol and aldehyde dehydrogenases and esophageal squamous cell carcinoma risk in males

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World Journal of Gastroenterology 2005年第33期11卷 5103-5108页

作者： Chia-fang Wu Deng-chyang Wu Hon-Ki Hsu Ein-Long Kao Jang-Ming Lee Cheng-Chieh Lin Ming-Tsang Wu Graduate Institute of Occupational Safety and Health and Department of Occupational Medicine Kaohsiung Medical University Kaohsiung Taiwan China Department of Chest Surgery Kaohsiung Veterans General Hospital KaohsiungTaiwan China Department of Chest Surgery Kaohsiung Medical University Hospital Kaohsiung Taiwan China Department of Surgery National Taiwan University Hospital Taipei Taiwan China Department of Family Medicine China Medical University and Hospital Taichung 404Taiwan China

AIM: To investigate the association between the genetic polymorphisms of ADH2 and ALDH2, lifetime alcohol consumption and esophageal cancer risk in the Taiwan Residents ***: Between August 2000 and June 2003, 134 pathologically-proven esophageal squamous cell carcinoma male patients and 237 male controls were recruited from Kaohsiung Medical University Hospital and Kaohsiung Veterans General Hospital in southern ***2 and ALDH2 polymorphisms were genotyped using ***: Compared to those with ADH2*2/*2,individuals with ADH2*1/*2 and ADH2*1/*1 had 2.28-and 7.14-fold, respectively, increased risk of developing esophageal cancer (95%CI = 1.11-4.68 and 2.76-18.46)after adjusting for alcohol consumption and other covariates. The significant increased risk was also noted among subjects with ALDH2*1/*2 (adjusted OR (AOR)= 5.25, 95%CI = 2.47-11.19), when compared to those with ALDH2*1/*1. The increased risk of esophageal cancer was made greater, when subjects carried both ADH2*1/*1 and ALDH2*1/*2, compared to those with ADH2*1/*2 or ADH2*2/*2 and ALDH2*1/*1 (AOR = 36.79,95%CI = 9.36-144.65). Furthermore, we found a multiplicative effect of lifetime alcoholic consumption and genotypes (ADH2 and ALDH2) on esophageal cancer ***: Our findings suggest that polymorphisms of ADH2 and ALDH2 can modify the influence of alcoholic consumption on esophageal cancer risk.

关键词：遗传因素基因多态性酒精乙醛脱氢酶食管鳞状细胞癌

CYP2C19 polymorphism influences Helicobacter pylori eradication

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World Journal of Gastroenterology 2014年第43期20卷 16029-16036页

作者： Chao-Hung Kuo Chien-Yu Lu Hsiang-Yao Shih Chung-Jung Liu Meng-Chieh Wu Huang-Ming Hu Wen-Hung Hsu fang-Jung Yu Deng-chyang Wu Fu-Chen Kuo Division of Gastroenterology Department of Internal MedicineKaohsiung Medical University Hospital Department of Medicine Faculty of MedicineCollege of Medicine and Center for Stem Cell ResearchKaohsiung Medical University Department of Internal Medicine Kaohsiung Municipal Hsiao-Kang Hospital Graduate Institute of Public Health Kaohsiung Medical University School of Medicine College of MedicineE-Da HospitalI-Shou University

The known factors that have contributed to the decline of Helicobacter pylori(***)eradication rate include antibiotic resistance,poor compliance,high gastric acidity,high bacterial load,and cytochrome P450 2C19(CYP2C19)*** pump inhibitor(PPI)is important in the eradication *** principal enzyme implicated in the metabolism of PPIs is *** effects of PPI depend on metabolic enzyme,cytochrome P450 enzymes,and CYP2C19 with genetic differences in the activity of this enzyme(the homozygous EM,heterozygous EM(Het EM),and poor metabolizer).The frequency of the CYP2C19 polymorphism is highly varied among different ethnic *** CYP2C19genotype is a cardinal factor of *** eradication in patients taking omeprazole-based or lansoprazolebased triple *** contrast,the CYP2C19 polymorphism has no significant effect on the rabeprazolebased or esomeprazole-based triple *** efficacy of levofloxacin-based rescue triple therapy might be also affected by the CYP2C19 polymorphism,but CYP2C19 genotypes did not show obvious impact on other levofloxacin-based rescue *** of different PPIs and/or increasing doses of PPIs should be individualized based on the pharmacogenetics background of each patient and pharmacological profile of each *** possible factors influencing gastric acid secretion(e.g.,IL-1β-511 polymorphism)would be also under consideration.

关键词： Helicobacter pylori CYP2C19 Polymorphism Eradicati