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Mapping of fruit apex shape related QTLs across multi-genetic backgrounds in cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2022年第3期8卷 328-340页

作者： Pinyu Zhu Yongjiao Meng Kaijing Zhang Xueyan Wang Kun Liang Tuantuan Wang Jian Xu Xiaodong Qin Zhe Wu Chunyan Cheng Xiaqing Yu chuntao qian Qunfeng Lou Jinfeng Chen Ji Li State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China College of Engineering/Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology&Equipment Nanjing Agricultural UniversityNanjing 210031China College of Agriculture Anhui Science and Technology UniversityFengyang 233100China

The shape of fruit apex is critical to appearance quality in cucumber(Cucumis sativus L.),of which the genetic basis was poorly understood,and the use of marker-assisted breeding for fruit apex improvement is not available *** this study,the variation of fruit apex in different cucumber ecotypes was evaluated by fruit apex angle(variation coefficient from 7.1%to 15.7%)and fruit apex index(variation coefficient from 8.8%to 22.6%).Fruit apex associated QTLs were mapped by using 145 F_(2:3) families and 155 F_(2:6) population that were derived from the cross of different ecotype *** of the mapping populations were conducted in four experiments in 2 *** majoreffect QTLs,Bfal4.1,Bfai4.1,Bfad6.1 and Bfai6.1 were consistently and reliably detected across two environments which could explain 11.6%-33.6%phenotypic variations(R^(2))in the F_(2:3) *** major-effect QTLs,Ofai4.1(R^(2)=13.4%-15.5%),Ofal4.1(R^(2)=10.7%-12.8%),and Ofad6.1(R^(2)=11.6%-12.4%)were stably detected in the F_(2:6) population in two ***4.1,Bfal4.1,Ofai4.1 and Ofal4.1 were integrated to be consensus QTL fa4.1,within which 11 candidate genes were ***6.1 and Bfad6.1 were integrated to be consensus QTL *** interaction analysis showed that Bfai6.1 has epistatic effect with *** study revealed two reliable major-effect fruit apex related QTLs across multi-genetic backgrounds and environments in *** possible candidate genes regulating the shape of fruit apex,and the relationship between cell division and fruit apex morphogenesis were discussed.

关键词： Cucumber Fruit apex Variation QTL Candidate gene

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不同套袋时间对甜瓜果实外观及品质的影响

上海农业学报 2024年第2期40卷 37-42页

作者：田守波钱春桃姚东伟刁倩楠曹燕燕戚欢军王楠张永平上海市农业科学院设施园艺研究所上海市设施园艺技术重点实验室上海201403 南京农业大学园艺学院作物遗传与种质创新国家重点实验室南京210095 上海湾区高新技术产业开发区农业农村服务中心上海201506 上海上实现代农业开发有限公司上海202183

以甜瓜18X-9和W4-3为试材,研究授粉后10 d和授粉后20 d两个套袋时间对甜瓜果实基础性状、质构特性和营养品质的影响,初步筛选甜瓜套袋的适宜时间,明确套袋对甜瓜果实外观和内在品质的影响。结果表明:套袋处理对2个甜瓜材料的果形指数影... 详细信息

以甜瓜18X-9和W4-3为试材,研究授粉后10 d和授粉后20 d两个套袋时间对甜瓜果实基础性状、质构特性和营养品质的影响,初步筛选甜瓜套袋的适宜时间,明确套袋对甜瓜果实外观和内在品质的影响。结果表明:套袋处理对2个甜瓜材料的果形指数影响不显著,授粉后10 d套袋处理的18X-9果肉厚度、可溶性固形物含量和单果重均高于对照,而授粉后20 d套袋的18X-9果肉厚度、可溶性固形物含量和单果重均显著低于对照。授粉后10 d套袋的W4-3果肉厚度和可溶性固形物含量显著高于其他2个处理,授粉后20 d套袋处理的W4-3除单果重外,果肉厚度和可溶性固形物含量均高于对照。套袋可以显著降低甜瓜的果肉硬度,对果皮硬度的影响较小;果实脆性显著增加,且授粉后10 d套袋效果最佳。授粉后10 d套袋处理的18X-9各品质指标均显著提高,其中,蔗糖和类黄酮含量增加最为显著;授粉后20 d套袋处理的18X-9除果糖和总酚外,其他品质指标值均高于对照。W4-3的2个套袋处理的果实品质均好于对照,其中授粉后10 d套袋对W4-3甜瓜果实品质提升的效果显著好于授粉后20 d套袋。可见,套袋处理对W4-3品质的促进作用好于18X-9,授粉后10 d套袋的效果最佳。

关键词：甜瓜套袋质构品质

Genome-Wide GRAS Gene Family Analysis Reveals the Classification,Expression Profiles in Melon(Cucumis melo L.)

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Phyton-International Journal of Experimental Botany 2021年第4期90卷 1161-1175页

作者： Yanfei Bi Bin Wei Ying Meng Zhongzhao Li Zhenghui Tang Feng Yin chuntao qian Jiangsu Changshu National Agricultural Science and Technology Park Suzhou215500China College of Horticulture Nanjing Agricultural UniversityNanjing210095China Changshu Agricultural Science and Technology Development Co. Ltd.Suzhou215500China

Melon(Cucumis melo),belonging to the Cucurbitaceae family,is a globally important economic ***(GAI,RGA,SCR)genes,which are a type of transcription factor,play a critical role in plant growth and development,including processes such as radial root patterning,light signalling,abiotic/biotic stress,axillary shoot meristem formation,and phytohormone(gibberellin)signal *** this study,the GRAS family in melon was analysed comprehensively with respect to chromosomal location,motif prediction,gene structure,and expression pattern.A total of 37 GRAS genes were first identified in melon,after which a phylogenetic tree was built with the GRAS genes of three model species(Arabidopsis,rice,and sacred lotus)and were divided into nine groups based on the findings of previous *** and gene structure analysis showed typical conserved domains in all melon GRAS and similar structures in the same *** expression analysis of GRAS genes done using RNA-seq data,showed that these genes were differentially expressed in different melon leaves under powdery mildew ***,the real-time quantitative PCR for GRAS genes revealed gene expression corresponding to powdery mildew *** results provide useful information for a better understanding of GRAS genes and provide the foundation for additional functional exploration of the melon GRAS gene family in the powdery mildew stress response.

关键词： Genome-wide identification GRAS melon stress response

Comparative Cyto-molecular Analysis of Repetitive DNA Provides Insights into the Differential Genome Structure and Evolution of Five Cucumis Species

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Horticultural Plant Journal 2019年第5期5卷 192-204页

作者： Shuqiong Yang Chunyan Cheng Xiaodong Qin Xiaqing Yu Qunfeng Lou Ji Li chuntao qian Jinfeng Chen State Key Lab of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing210095China Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province School ofAgricultural EngineeringNanyang Normal UniversityNanyang473061China

The genus Cucumis, includes the cucumber(2n = 14), melon(2n = 24), and other wild species, which is a good model for studying genome organization and evolution due to their variation in genome size and basic chromosome number. In this study, five Cucumis species with different geographical origins and basic chromosome numbers(i.e., C. sativus, C. hystrix, C. melo, C. anguria, and C. metuliferus) were used to identify and characterize the repetitive DNA in detail using a phylogenetic method. Comparative cyto-molecular genetic analysis of repetitive DNA was carried out using a graph-based clustering method, construction of Neighbor-Joining tree and fluorescence in situ hybridization(FISH).The results revealed that the five Cucumis species had differences in the repeat content of their genome, as well as in the composition of repetitive DNA and their genomic proportions. Three species from subgenus Melo showed a decreased tendency in both repeat types and genomic proportions, while two species from subgenus Cucumis also showed a decreased tendency in repeat types, but an increased tendency in genomic proportions. Phylogenic analysis of Cucumis Ty1/Copia, Ty3/Gypsy, and 45 S rDNA ITS regions revealed that C. sativus, C. hystrix, and C. melo were closely related species, which C. sativus and C. hystrix were closer, while C. anguria and C. metuliferus were closer to each other and further from the other three species. Differential accumulation and elimination of different repeat types divergently shaped the genomic architecture of these five Cucumis species, contributing to the genome’s evolution and diversification. Overall, these results enhance our understanding of the genomes of these five Cucumis species, and contribute to a more holistic view of genome evolution and phylogenetics of this genus.

关键词： Cucumis repetitive sequence comparative genomics FISH phylogenetic evolution

A historical review of hybrid rice breeding

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Journal of Integrative Plant Biology 2024年第3期66卷 532-545页

作者： Xiaoming Zheng Fei Wei Cheng Cheng qian qian State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Yazhouwan National Laboratory Sanya City 572024China

The development of germplasm resources and advances in breeding methods have led to steady increases in yield and quality of rice (Oryza sativa L.). Three milestones in the recent history of rice breeding have contributed to these increases: dwarf rice breeding, hybrid rice breeding, and super rice breeding. On the 50th anniversary of the success of three-line hybrid rice,we highlight important scientific discoveries in rice breeding that were made by Chinese scientists and summarize the broader history of the field. We discuss the strategies that could be used in the future to optimize rice breeding further in the hope that China will continue to play a leading role in international rice breeding.

关键词： Chinese experience dwarf breeding hybrid rice breeding super rice breeding

Special issue:Catenary optics and catenary electromagnetics

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Opto-Electronic Advances 2024年第5期7卷 1-4页

作者： qian Sun Minghui Hong Pen-Tung Sah Institute of Micro-Nano Science and Technology Xiamen UniversityXiamen 361005China Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) Xiamen 361005China

As a natural mathematical form,a catenary is the curve that a hanging chain or cable adopts under its own weight when supported at its ends in a homogeneous gravitational *** unique shape arises from the balance of forces acting on each segment of the chain,resulting in a curve described by hyperbolic cosine *** functions play pivotal roles in describing the electromagnetic field,intensity distribution,and dispersion of structured light on the sub-wavelength scale^(1).

关键词： functions. field. optics

Molecular Mechanism of Rice Necrotic Lesion for Optimized Yield and Disease Resistance

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Rice science 2024年第3期31卷 285-299页

作者： HOU Xinyue WANG Yuping qian qian REN Deyong State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Rice Research InstituteSichuan Agricultural UniversityChengdu 611130China

How to balance rice resistance and yield is an important issue in rice *** with mutated necrotic lesion genes often have persistent broad-spectrum resistance,but this broad-spectrum resistance usually comes at the expense of ***,many necrotic lesion mutants in rice have been identified,and these genes are involved in disease resistance *** review provides a detailed introduction to the characteristics,classification,and molecular mechanisms of necrotic lesion ***,we review the molecular regulatory pathways of genes involved in rice disease ***,we summarize the relationship between resistance and yield in rice using newly developed gene editing *** discuss a rational and precise breeding strategy to better utilize molecular design technology for breeding disease-resistant and high-yield rice varieties.

关键词： rice necrotic lesion regulation mechanism high yield high resistance

Localizing vitamin-C through nanoconfinement enables high-rate CO_(2) electrolysis to ethylene

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Science China Materials 2024年第3期67卷 1006-1008页

作者： Zhengyi qian Shaojun Guo School of Materials Science and Engineering Peking UniversityBeijing 100871China

The electrosynthesis of value-added fuels and chemicals from carbon dioxide reduction reaction(CO_(2)RR)is a promising approach for realizing the objectives of carbon neutrality[1].Nevertheless,the current production of desirable multicarbon(C_(2+))chemicals,such as ethylene(C_(2)H_(4)),still encounters challenges related to low selectivity,low current density,and the requirement for high-purity feeding gas[2].

关键词： enable purity dioxide

The tangential k-Cauchy-Fueter type operator and Penrose type integral formula on the generalized complex Heisenberg group

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Applied Mathematics(A Journal of Chinese Universities) 2024年第1期39卷 181-190页

作者： REN Guang-zhen SHI Yun KANG qian-qian Department of Mathematics Zhejiang International Studies UniversityHangzhou 310023China Department of Mathematics Zhejiang University of Science and TechnologyHangzhou 310023China

The tangential k-Cauchy-Fueter operator and k-CF functions are counterparts of the tangential Cauchy–Riemann operator and CR functions on the Heisenberg group in the theory of several complex variables,*** this paper,we introduce a Lie group that the Heisenberg group can be imbedded into and call it generalized complex *** investigate quaternionic analysis on the generalized complex *** also give the Penrose integral formula for k-CF functions and construct the tangential k-Cauchy-Fueter complex.

关键词： the generalized complex Heisenberg group the tangential k-Cauchy-Fueter type operator Penrose-type integral formula