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ERF5 enhances protocorm-like body regeneration via enhancement of STM expression in Dendrobium orchid

Journal of Integrative Plant Biology 2023年第9期65卷 2071-2085页

作者： Danqi Zeng Can Si Mingze Zhang Jun Duan chunmei he Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong Provincial Key Laboratory of Applied Botany South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China University of the Chinese Academy of Sciences Beijing 100049China South China National Botanical Garden Guangzhou 510650China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesGuangzhou 510650China

Orchid plants develop protocorms upon germination and produce protocorm-like structures called protocorm-like bodies(PLBs)from protocorms and somatic cells via tissue ***-like bodies have broad technical application potential in the orchid industry and their regeneration is a distinct developmental process in the plant ***,little is known about this unparalleled developmental *** this study,we identified a PLB-abundant gene,ethylene response factor(ERF),and a transcription factor named Do ERF5,and determined its important role in PLB regeneration in Dendrobium *** of Do ERF5 in Dendrobium greatly enhanced the PLB regeneration from PLB and stem explants,and upregulated the expression of WOUND-INDUCED DEDIFFERENTIATION(Do WIND)homologs and SHOOT MERISTEMLESS(Do STM),as well as the genes involved in cytokinin biosynthesis(Do IPT)and the cytokinin response factors(Do ARRs).However,silencing Do ERF5 reduced the regeneration rate of PLBs,and downregulated the expression of Do WIND homologs,Do STM and Do *** demonstrated that Do ERF5 is directly bound to the Do STM promoter and regulates its *** addition,overexpression of Do STM in Dendrobium orchid resulted in favorable regeneration of *** results clarify that Do ERF5 regulates the regeneration of PLB by enhancing Do STM *** findings provide new insights into how Do ERF5 mediates PLB regeneration and offers technical potential in improving clonal propagation,preservation,and the bioengineering of orchids.

关键词： EMSA ERF transcription factor orchid protocormlikebody STM

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GSK3-mediated stress signaling inhibits legume-rhizobium symbiosis by phosphorylating GmNSP1 in soybean

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Molecular Plant 2021年第3期14卷 488-502页

作者： chunmei he Hui Gao Haijiao Wang Yun Guo Miao he Yaqi Peng Xuelu Wang National Key Laboratory of Crop Genetic Improvement Center of Integrative BiologyCollege of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475001China

Legumes establish symbiotic associations with rhizobia for biological nitrogen *** process is highly regulated by various abiotic stresses,but the underlying genetic and molecular mechanisms remain largely *** this study,we discovered that the glycogen synthase kinase 3(GSK3)-like kinase,GmSK2-8,plays an important role in inhibiting symbiotic signaling and nodule formation in soybean(Glycine max)under salt *** found that GmSK2-8 is strongly induced in soybean under high-salt conditions,while GmSK2-8 could interact with two *** Nodulation Signaling Pathway 1(GmNSP1)proteins,GmNSP1a and GmNSP1b;these key transcription factors are essential for rhizobial infection,nodule initiation,and symbiotic gene expression in ***,we demonstrated that GmSK2-8 phosphorylates the LHRI domain of GmNSP1a,inhibits its binding to the promoters of symbiotic genes,and thus suppresses nodule formation under salt *** of GmSK2-8 and its close homologs also resulted in reduced plant sensitivity to salt stress during nodule *** together,our findings indicate that GSK3-like kinases directly regulate the activities of GmNSP1s to mediate salt-inhibited legume–rhizobium symbiosis,providing novel targets for improving symbiotic nitrogen fixation under environmental stress conditions in soybean and possibly other legumes.

关键词： nodule formation rhizobial infection salt stress soybean symbiosis

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GmBES1-1 dampens the activity of GmNSP1/2 to mediate brassinosteroid inhibition of nodulation in soybean

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Plant Communications 2023年第6期4卷 244-258页

作者： Xu Chen Xiaotong Hu Haijiao Wang Jing Liu Yaqi Peng chunmei he Miao he Xuelu Wang Center of Integrative Biology College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityZhengzhou 450046China Academy for Advanced Interdisciplinary Studies Henan UniversityZhengzhou 450046China Sanya Institute of henan University Sanya 572025China

Soybean(Glycine max)forms root nodules to house rhizobial bacteria for biological nitrogen *** development of root nodules is intricately regulated by endogenous and exogenous *** phytohormones brassinosteroids(BRs)have been shown to negatively regulate nodulation in soybean,but the underlying genetic and molecular mechanisms remain largely ***,we performed transcriptomic analyses and revealed that BR signaling negatively regulates nodulation factor(NF)*** found that BR signaling inhibits nodulation through its signaling component GmBES1-1 by dampening NF signaling and nodule *** addition,GmBES1-1 can directly interact with both GmNSP1 and GmNSP2 to inhibit their interaction and the DNA-binding activity ***,BR-induced nuclear accumulation of GmBES1-1 is essential for inhibiting *** together,our results demonstrate that regulation ofGmBES1-1 subcellular localization by BRs plays a key role in the legume–rhizobium symbiosis and plant development,indicating a crosstalk mechanism between phytohormone and symbiosis signaling pathways.

关键词： nodulation brassinosteroid crosstalk GmBES1-1 GmNSP1/2

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A new vegetation index combination for leaf carotenoid-tochlorophyll ratio: minimizing the effect of their correlation

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International Journal of Digital Earth 2023年第1期16卷 272-288页

作者： chunmei he Jia Sun Yuwen Chen Lunche Wang Shuo Shi Feng Qiu Shaoqiang Wang Torbern Tagesson Hubei Key Laboratory of Regional Ecology and Environmental Change School of Geography and Information EngineeringChina University of GeosciencesWuhanPeople’s Republic of China Department of Physical Geography and Ecosystem Science Lund UniversityLundSweden State Key Laboratory of Information Engineering in Surveying Mappingand Remote SensingWuhan UniversityWuhanPeople’s Republic of China International Institute for Earth System Science Jiangsu Provincial Key Laboratory of Geographic Information Science and TechnologyNanjing UniversityNanjingPeople’s Republic of China Department of Geosciences and Natural Resource Management University of CopenhagenCopenhagenDenmark

The ratio of leaf carotenoid to chlorophyll(Car/Chl)is an indicator of vegetation photosynthesis,development and responses to ***,the correlation between Car and Chl,and their overlapping absorption in the visible spectral domain pose a challenge for optical remote sensing of their *** study aims to investigate combinations of vegetation indices(VIs)to minimize the influence of Car-Chl correlation,thus being more sensitive to the variability in the ratio across vegetation species and *** sensitive to Car and Chl variability were combined into four candidates of combinations,using a simulated dataset from the PROSPECT *** VI combinations were then tested using six simulated datasets with different Car-Chl correlations,and evaluated against four independent *** ratio of the carotenoid triangle ratio index(CTRI)with the red-edge chlorophyll index(CIred-edge)was found least influenced by the Car-Chl correlation and demonstrated a superior ability for estimating Car/Chl *** with published VIs and two machine learning algorithms,CTRI/CIred-edge also showed the optimal performance in the fourfield *** new VI combination could be useful to provide insights in spatiotemporal variability in the leaf Car/Chl ratio,applicable for assessing vegetation physiology,phenology,and response to environmental stress.

关键词： Leaf carotenoids content leaf chlorophyll content PROSPECT model ratio of Car to Chl vegetation index

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Mechanical behaviours of sandstone containing intersecting fissures under uniaxial compression

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Journal of Rock Mechanics and Geotechnical Engineering 2022年第2期14卷 460-476页

作者： Fei Xiong Xinrong Liu Xiaohan Zhou Guangyi Lin Dongshuang Liu Yafeng Han Bin Xu chunmei he Zijuan Wang School of Civil Engineering Chongqing UniversityChongqing400045China State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing400044China National Joint Engineering Research Center of Geohazards Prevention in the Reservoir Areas Chongqing UniversityChongqing400045China State Key Laboratory for Geomechanics and Deep Underground Engineering China University of Mining and TechnologyXuzhou221116China College of Architectural Engineering Neijiang Normal UniversityNeijiang641100China School of Management Science and Engineering Chongqing Technology and Business UniversityChongqing400067China

Predicting rock cracking is important for assessing the stability of underground *** effects of the intersecting angle a and the distribution orientation angleβof intersecting fissures on the uniaxial compressive strength and the failure characteristics of sandstone containing intersecting fissures are investigated through laboratory experiments and two-dimensional particle flow code(PFC2D).The relationship between the mechanical properties of sandstone and the intersecting angle a and the distribution orientation angleβis *** initiation forms and the final failure modes are then categorised and determined via empirical *** addition,the cracking processes of intersecting fissures with different a andβvalues are *** results show that variations in the peak stress,peak strain,average modulus,and crack initiation stress of sandstone containing intersecting fissures show a“moth”shape in the space of the a-β-mechanical *** crack initiation forms are identified:inner tip cracking(usually accompanied by one outer tip cracking)and only outer tips *** failure modes are observed:(1)the main fracture planes are created at the inner tip and one outer tip,and(2)the main fracture planes are formed at the two outer *** main crack evolution processes of sandstone containing intersecting fissures under uniaxial compression are *** for quickly determining the crack initiation form and the failure mode are *** combination of the determination equations for the crack initiation form and the failure mode can be used to predict the crack *** approach for determining the crack evolution processes is hence proposed with acceptable precision.

关键词： Intersecting fissures Sandstone Uniaxial compression Fracture determination Cracking evolution

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Co-incorporation of Chinese milk vetch(Astragalus sinicus L.)and chemical fertilizers alters microbial functional genes supporting short-time scale positive nitrogen priming effects in paddy soils

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Pedosphere 2024年第3期34卷 567-576页

作者： Limin WANG chunmei he Dongfeng HUANG Juhua YU Cailing LIU Qinghua LI Yibin HUANG Juan LI Fei WANG Institute of Resources Environment and Soil FertilizerSoil and Fertilizer InstituteFujian Academy of Agricultural SciencesFuzhou 350012(China) School of Resources and Environment Engineering Anhui UniversityHefei 230601(China)

Nitrogen(N)priming is a microbially mediated biochemical process as affected by different incorporation ***,little information is known about the microbial mechanisms driving the response of N priming to co-operation of Chinese milk vetch(CMV,Astragalus sinicus L.)and different rates of chemical fertilizers in paddy soils in South ***,an anaerobic incubation experiment was conducted to study N priming effects(PE)and their relationships with soil microbial functional genes after CMV incorporation alone(M),co-incorporation of CMV with 100%(normal dosage)chemical fertilizers(MC100),and co-incorporation of CMV with 80% chemical fertilizers(MC80).Co-incorporation of CMV and chemical fertilizers enhanced the short-time scale(the first 20 d of incubation)positive PE of N,while no significant differences existed among the three treatments on day 60 or 90 of incubation(P>0.05).Compared with the M treatment,gross priming effect(GPE)in the MC100 and MC80 treatments significantly increased by 34.0% and 31.3%,respectively,and net priming effect(NPE)increased by 47.7% and 47.8%,respectively,during the first 20 d of incubation(Phe gdhA gene abundance by 5.0% and 9.8%,increased the gdh2 gene abundance by 12.7% and 45.7%,and increased the nasB gene abundance by 9.5% and 41.4%,respectively,in comparison with the M treatment on day 20 of *** analyses indicated that soil microbial functional genes involved in N mineralization(gdhA and gdh2),assimilatory nitrate reduction(nasB),and nitrification(amoB)were significantly correlated with N priming under different incorporation practices during the incubation period(Phemical fertilizers can regulate soil microbial community functional gene structure,which may accelerate mineralization and assimilatory nitrate reduction and inhibit

关键词： green manure gross priming effect incorporation practice microbial mechanism net priming effect rice field soil

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Functional analysis of flavanone 3-hydroxylase(F3H)from Dendrobium officinale,which confers abiotic stress tolerance

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Horticultural Plant Journal 2023年第2期9卷 356-364页

作者： Can Si Wei Dong Jaime A.Teixeira da Silva chunmei he Zhenming Yu Mingze Zhang Lei Huang Conghui Zhao Danqi Zeng Chuanmao Li Shengxiang Qiu Jun Duan Key laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong Provincial Key laboratory of Applied BotanySouth China Botanical GardenChinese Academy of SciencesGuangzhouGuangdong 510650China University of Chinese Academy of Sciences Beijing 100049China College of Life Science and Technology Xi An Jiao Tong UniversityXi’anShaanxi 710049China Independent Researcher Miki-choKagawa-kenJapan Guangzhou Keneng Cosmetic Scientific Research Co. LtdGuanghzouGuangdong 510650China

Flavonoids are important bioactive components in Dendrobium officinale,a medicinal *** are involved in many biological activities,including protecting plants against biotic and abiotic *** on the key genes related to flavonoid biosynthesis in *** is *** this study,one of the key flavonoid biosynthesis genes,flavanone 3-hydroxylase(F3H),was characterized from *** open reading frame of DoF3H was 1134 bp long and it encoded a 377-amino acid *** DoF3H protein showed considerably high homology with F3H proteins from other plant species and shared a common evolutionary ancestor with other ***3H transcripts were detected in different organs of adult plants and mainly accumulated in flowers,followed by roots,stems and leaves,a pattern that was similar to the content of *** DoF3H protein,which was localized in the cytosol,could convert naringenin to *** mRNA levels of DoF3H were significantly induced by salt and cold ***,the heterologous expression of DoF3H in Escherichia coli conferred it higher tolerance to salt and cold *** results provide insight into the molecular function of DoF3H in the biosynthesis of flavonoids,and provide a new application for improvement of abiotic tolerance in ***.

关键词： Dendrobium officinale Flavanone 3-hydroxylase Dihydrokaempferol Salt stress Cold stress

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过程性评价、成就目标定向与学习投入:机制与路径

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高教探索 2020年第11期 36-46页

作者：何春梅四川大学锦城学院通识教育学院成都611731

作为重要的教学情境,过程性评价对学习动机及学习投入的影响尚缺乏关注,明确其杠杆机制有助于在教学管理中实施良性干预。采取随机抽样的方式,以某二本院校609名学习者为研究对象获得样本数据。通过相关性分析、验证性因子分析及多重中... 详细信息

作为重要的教学情境,过程性评价对学习动机及学习投入的影响尚缺乏关注,明确其杠杆机制有助于在教学管理中实施良性干预。采取随机抽样的方式,以某二本院校609名学习者为研究对象获得样本数据。通过相关性分析、验证性因子分析及多重中介效应检验后发现:(1)过程性评价显著正向影响学习投入;(2)掌握目标定向、成绩趋近目标定向和成绩回避目标定向对学习投入存在显著正向影响;(3)掌握目标定向和成绩趋近目标定向在过程性评价与学习投入的关系中起部分中介作用,成绩回避目标定向的中介效应不显著。以上结果表明,过程性评价不仅能直接预测,还能通过成就目标定向的中介作用来间接预测学习投入,所以应优化评价方式,利用成就目标定向的导向性,促进学习投入。

关键词：过程性评价学习投入成就目标定向中介效应影响机制

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A novel gene carrier based on amino-modified silica nanoparticles

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Science Bulletin 2003年第3期48卷 223-228页

作者： he Xiaoxiao, WANG Kemin, TAN Weihong, LIU Bin, LIN Xia, HUANG Shasheng, LI Du, he chunmei & LI Jun State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technol-ogy, Hunan University, Changsha 410082, China State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry & Chemical Engineering Institute of Biological Technology Hunan University Changsha China

Uniform-sized amino-modified silica nanopar-ticles have been prepared by the controlled synchronous hydrolysis of tetraethoxysilane and N-(?amimoethyl)-- aminopropyltriethoxysilane in water nanodroplet of the wa-ter-in-oil microemulsion. These nanoparticles display posi-tive charge potential at definited pH. This is due to the pres-ence of amino groups on the surface of the nanoparticles. Nanoparticles-plasmid DNA complexes can easily form through electrostatical binding between the positive charges of the amino-modified silica nanoparticles and the negative charges of the plasmid DNA. The complexes can be also dis-sociated under alkaline pH or high ionic strength conditions. And enzymatic digestion of the plasmid DNA is almost in-hibited by these nanoparticles complexes. A novel non-viral gene carrier based on the amino-modified silica nanoparti-cles is proposed under the combination of nanotechnology, biotechnology and gene engineering technology. The plasmid DNA can successfully cross various systemic barriers to COS-7 cells as well as mediate high expression of Green Fluorescence Protein (GFP) gene in cells by use of this novel gene carrier.

关键词： gene carrier nanoparticle amino-modified silica.

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Overexpression of GDP-mannose transporter DoGMT1 in Dendrobium officinale increases mannan accumulation

Overexpression of GDP-mannose transporter DoGMT1 in Dendrobi...

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2018全国植物生物学大会

作者： Zhenming Yu Ziyin Yang chunmei he Jun Duan Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement Guangdong Provincial Key Laboratory of Applied BotanySouth China Botanical GardenChinese Academy of Sciences

Mannan polysaccharide,a potential candidate for drugs,has received widespread attention due to its remarkable pharmacological activities with fewer side *** officinale,a traditional Chinese orchid herb,accumulates abundant mannan polysaccharide in its ***-mannose serves as an oligosaccharide donor for the biosynthesis of mannan polysaccharide,however,the mechanism regarding how these GDP-mannoses are transported into Golgi for the biosynthesis of mannan polysaccharide remains *** present study,a GDP-mannose transporter(DoGMT1) was isolated from Dendrobium officinale.A positive correlation between transcript levels of DoGMT1 and mannan content was found during plant ***1 was targeted to Golgi apparatus,and showed the highest expression level in stems.A GDP-binding motif GXVNK,transporting GDP-mannose into Golgi lumen,was evolutionarily conserved in ***,DoGMT1 complemented a GDP-mannose transport-defective yeast mutant(vrg4-2) with corresponding phenotype,meanwhile it exhibited a higher GDP-mannose uptake *** plants overexpressing the DoGMT1 indicated increased mannose-containing polysaccharide,which resulted from a dramatic increase of the compositional *** findings demonstrated that DoGMT1,primarily transferring GDP-mannose into Golgi lumen,is required for mannan biosynthesis in ornamental plant Dendrobium officinale.

关键词： nucleotide sugar transporter mannan GDP-mannose Golgi lumen

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