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Contrasting Drought Tolerance in Two Apple Cultivars Associated with Difference in Leaf Morphology and Anatomy

American Journal of Plant Sciences 2019年第5期10卷 709-722页

作者： Tuanhui Bai Zhanying Li chunhui song Shangwei song Jian Jiao Yuchen Liu Zhidan Dong Xianbo Zheng College of Horticulture Henan Agricultural University Zhengzhou China Henan Key Laboratory of Fruit and Cucurbit Biology Zhengzhou China

Apple is one of the most important fruit trees in temperate zones, and is cultivated widely throughout the world. Drought stress affects the normal growth of apple tree, and further affects fruit yield and quality. The present study examined the effects of drought on photosynthesis and water use efficiency (WUE) of two apple cultivars (Honeycrisp and Yanfu 3) that differ in drought tolerance. The results showed that the photosynthetic rate decreased in response to drought stress for both cultivars, with significant differences in intensity. Values for net photosynthetic rate (Pn) in stressed Yanfu 3 remained significantly lower than in the controls, while, for Honeycrisp, only a slight drop in photosynthesis. Similarly, stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr) were markedly reduced in Yanfu 3 under drought stress. However, Honeycrisp showed only minor changes. Under drought stress, the contents of Chl a, Chl b and Chl t in Yanfu 3 were all decreased significantly compared with the control. However, little difference in Honeycrisp was noted between stressed plants and controls. Values for WUE in stressed Yanfu 3 remained higher than in the controls from day 3 until the end of the experiment, while no significant difference was observed in Honeycrisp. Furthermore, Honeycrisp also exhibited superior physiological traits, as indicated by its anatomical and morphological characteristics. Therefore, we conclude that the superior drought tolerance of Honeycrisp was due to its anatomical and morphological characteristics, which possibly contributed to the maintenance of higher photosynthetic capacity than Yanfu 3.

关键词： Apple Drought Stress Photosynthesis Water Use Efficiency Leaf Anatomy

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电场强度对原油乳化液破乳脱水的影响

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高电压技术 2014年第1期40卷 173-180页

作者：陈庆国梁雯宋春辉哈尔滨理工大学工程电介质及其应用教育部重点实验室哈尔滨150080

为选择合适的脱水电场以提高对原油乳化液的脱水效率,试验研究了不同电场强度下乳化液的脱水效率。通过水滴极化模型的建立,推导出水滴在电场中的极化电荷量及水滴间的聚结力,并得到水滴破裂的临界电场强度表达式,以此在理论上确定了最... 详细信息

为选择合适的脱水电场以提高对原油乳化液的脱水效率,试验研究了不同电场强度下乳化液的脱水效率。通过水滴极化模型的建立,推导出水滴在电场中的极化电荷量及水滴间的聚结力,并得到水滴破裂的临界电场强度表达式,以此在理论上确定了最佳脱水电场强度。研究结果表明:水滴聚结的偶极力与外施电场强度、水滴半径和水滴间距有关,不同半径和间距的水滴存在不同的临界脱水电场强度;当外施电场强度小于临界电场强度时,随着外施电场强度的增加,脱水率呈上升趋势,但当外施电场强度超过水滴破裂临界电场强度时,脱水率随电场强度的增加而下降。得到的临界脱水电场强度表达式与试验结果的一致性较好,可作为确定最佳脱水电场强度的理论依据。

关键词：原油脱水乳化液电场强度极化破乳聚结

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Traits-based integration of multi-species inoculants facilitates shifts of indigenous soil bacterial community

Traits-based integration of multi-species inoculants facilit...

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中国土壤学会土壤环境专业委员会第二十次会议暨农田土壤污染与修复研讨会

作者： Jingjing Wang Qingqing Li song Xu Wei Zhao Yu Lei chunhui song Zhiyong Huang Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Core Facility Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Key Laboratory of Western China's Mineral Resources of Gansu Province School of Earth Sciences University of Lanzhou

Microbial co-inoculation is considered to be an innovative approach and had been applied worldwide. However, the underlying mechanisms of microbial co-inoculants constructions, especially the traits-based combination of distinctly different microbial species remains poorly understood. In this study, we constructed two microbial co-inoculants with the same three strains with emphasis on the microbial, soil and plant traits. Microbial co-inoculants 1(M1) were constructed according to soil fertility, microbial activity and cucumber nutrient requirement with a 2:1:2 ratio(Ensifer sp. NYM3, Acinetobacter sp. P16 and Flavobacterium sp. KYM3), while microbial co-inoculants 2(M2) were constructed according to soil fertility and cucumber nutrient requirement with a 1:10:1 ratio without considering the difference in the nutrient supply capability of microbial species. The results showed that M1 and M2 both obviously increased cucumber yields. The M1 had significant highest p H value, total nitrogen(TN) and Invertase activity(IA). The M2 had significant highest available phosphate(AP), NO-N, urea activity(UA) and Alkaline phosphatase activity(APA). Gammaproteobacteria, Acidobacteria, Nitrospirae and Armatimonadetes were significantly increased, while Actinobacteria and Firmicutes were significantly decreased by microbial co-inoculations(M1 and M2). The bacterial lineages enriched in M1 were Gammaproteobacteria and TM7. Acidobacteria, Bacteroidetes, and Deltaproteobacteria were enriched in M2. PCo A analysis showed that the bacterial communities were strongly separated by the different microbial inoculation treatments. The functional groups of intracellulararasites were highest in M1. The functional groups of phototrophy, photoautotrophy, nitrification, fermentation, cyanobacteria, oxygenichotoautotrophy, chitinolysis and animalarasitesrymbionts were highest in M2. Based on correlation analysis, it inferred that the M1 and M2 might promote cucumber yields by mediating bacterial

关键词： Microbial co-inoculants Integration Bacterial community structure and function Soil properties Soil enzyme Cucumber

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高职院校专业设置与产业需求协调发展实证研究——以吉林省为例

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高等工程教育研究 2017年第4期65卷 192-197页

作者：赵淑梅宋春辉吉林省教育科学院高教所吉林省教育厅职业与成人教育处

调研得知:吉林省产业结构在历史沿革中形成了产业结构重型化、产业结构内部转换中"非均衡性"特征。同时,高职院校专业建设质量参差不齐,整体结构不协调,形成了以第三产业相关专业为主导、三二产业专业为主、一产业专业相当薄弱的发展态... 详细信息

调研得知:吉林省产业结构在历史沿革中形成了产业结构重型化、产业结构内部转换中"非均衡性"特征。同时,高职院校专业建设质量参差不齐,整体结构不协调,形成了以第三产业相关专业为主导、三二产业专业为主、一产业专业相当薄弱的发展态势。而且,一、二、三产业专业结构与其相应产业需求存在不同程度的不匹配问题,应通过增强专业结构优化的整体意识、创新调控的方式方法、建立"三重"专业动态调整机制、增加专业建设经费等举措,推动专业设置与产业需求协调发展。

关键词：高职院校专业设置产业需求协调发展

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Intensified tectonic deformation and uplift of the Altyn Tagh range recorded by rock magnetism and growth strata studies of the western Qaidam Basin, NE Tibetan Plateau

Intensified tectonic deformation and uplift of the Altyn Tag...

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2015中国地球科学联合学术年会

作者： Tao Zhang Wenxia Han Xiaomin Fang Weilin Zhang chunhui song Maodu Yan Key Laboratory of Continental Collision and Plateau Uplift Institute of Tibetan Plateau Research CAS Key Laboratory of Salt Lake Resources and Chemistry Qinghai Institute of Salt Lakes Chinese Academy of Sciences CAS Center for Excellence in Tibetan Plateau Earth Sciences School of Earth Sciences & Key Laboratory of Western China’s Mineral Resources of Gansu Province

The Altyn Tagh range is the tectonically and geographically northern edge of the Tibetan Plateau,precise dating of its activities is essential for understanding the possible mechanisms of the Tibetan Plateau uplift and its effects on climate *** the framework of basin-mountain coupling,both magnetic susceptibility and rock magnetic research were carried out on the Late Cenozoic sediments of the Honggouzi section（7-5 Ma）in the western Qaidam *** study attempts to find out the evolution history of tectonic and climatic changes and to detect their interactions in the Altyn Tagh *** results showed that magnetic susceptibility records kept relatively low and stable values before0Ma,and rapidly increased since0 *** rock magnetic results revealed that paramagnetic minerals,maghemite and hematite have been dominated during the early periods（>10 Ma）in the Honggouzi section,and were replaced by magnetite and maghemite after that time.A detailed comparison of magnetic susceptibility records in the Honggouzi section with tectonic and climate records from the study areas and surrounding regions were also carried *** with sedimentary facies,lithology and angular unconformity in the sequence,as well as seismostratigraphy data,paleocurrent study,provenance and conceptual models analyses,we argue that the0 Ma shift in the HGZ section might be caused by the uplift of the Altyn Tagh range during that time.

关键词： Rock magnetism growth strata tectonic uplift Altyn Tagh range Qaidam Basin

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Genome Wide Transcriptomic Analysis of WRKYGene Family Response to Biotic Stresses in Malus×domestica

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American Journal of Plant Sciences 2021年第5期12卷 858-885页

作者： Hanghang Zhang Liu Yang Ruli Zhou Yingli Zhang Zhengyang Wang Hongguang Pang Tuanhui Bai Shangwei song Jian Jiao Miaomiao Wang Jiancan Feng chunhui song Xianbo Zheng College of Horticulture Henan Agricultural University Zhengzhou China

Apple (Malus × domestica Borkh.) is a perennial woody plant that often suffers from various biological stresses. Many harmful pathogens can infect apple trees and lead to reduced production. We comprehensively identified the WRKY genes in the apple genome and analyzed their expression in response to several biological stressors, including Alternaria alternata, Pythium ultimum, Botryosphaeria dothidea, Erwinia amylovora, Penicillium expansum, Gymnosporangium yamadae, and Apple replant disease. There were 113 MdWRKYs identified in the apple genome. Twenty-two MdWRKYs were differentially expressed in response to at least five pathogens. Promoter sequence analysis showed that these genes carried many defense- and stress-responsive elements, such as MeJA-response elements, salicylic acid-response elements, and W-box elements, in their promoters. Transient expression assays showed that MdWRKY

关键词： Apple WRKY Transcription Factor Pathogens Big Data

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Paleogeography control of Indian monsoon intensification and expansion at 41 Ma

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Science Bulletin 2021年第22期66卷 2320-2328,M0004页

作者： Xiaomin Fang Maodu Yan Weilin Zhang Junsheng Nie Wenxia Han Fuli Wu chunhui song Tao Zhang Jinbo Zan Yongpeng Yang State Key Laboratory of Tibetan Plateau Earth System Resources and EnvironmentInstitute of Tibetan Plateau Research(TPESRE)Chinese Academy of SciencesBeijing 100101China Key Laboratory of Continental Collision and Plateau Uplift Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China Key Laboratory of Western China’s Environments of Ministry of Education Lanzhou UniversityLanzhou 730000China Shandong Provincial Key Laboratory of Water and Soil Conservation&Environmental Protection School of Resource and Environmental SciencesLinyi UniversityLinyi 276000China School of Earth Sciences&Key Laboratory of Western China’s Mineral Resources of Gansu Province Lanzhou UniversityLanzhou 730000China

As a crucial part of the Asian monsoon stretching from tropical India to temperate East Asia, the Indian monsoon(IM) contributes predominant precipitation over Asian continent. However, our understanding of IM’s onset, development and the underlying driving mechanisms is limited. Increasing evidence indicates that the IM began in the Eocene or even the Paleocene and was unexceptionally linked to the early rise of the Tibetan Plateau(TP). These were challenged by the heterogeneous and diachronous uplift of the TP and all the reported records were confined to tropical zone under tropical monsoon driven by the Intertropical Convergence Zone(ITCZ) that is irrelevant to the TP. Therefore, reliable paleoclimatic records from the extra-tropical IM region is crucial to reveal how the tropical IM expanded to subtropical and temperate zones and what driving factors might be related to it. Here we present robust Eocene paleoenvironmental records from central Yunnan(~26°N) in subtropical East Asia. The multiproxy results of two sites demonstrate a consistent sudden switch from a dry environment in the early Eocene to a seasonally wet one at 41 Ma, suggesting a jump of the tropical IM to the southern subtropical zone at 41 *** full collision of India with Asia, and the resulting changes in paleogeography at 41 Ma(closure of the Neotethys sea, retreat of the Paratethys seas, fast northward movement of the southern margin of the TP and rise of the central TP), aided by synchronous Antarctic cooling, might have worked together to drive the IM enhancement and northward expansion.

关键词： Asia paleogeography Indian tropical monsoon Eocene paleoenvironment Subtropical monsoon onset Driven factors and forcings

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天然气长输管道输差控制与分析

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油气储运 2007年第4期26卷 44-47页

作者：王力勇宋春慧中国石化中原油田济南天然气管输处

针对目前天然气管输企业发展过程中存在的输差控制问题,指出应建立天然气集输平衡与输差分析机制,并分析了其影响因素。在当前技术及管理现状的条件下对输差、输差定位基础工作、输差分析与输差控制等问题进行了探讨,提出了天然气输差... 详细信息

针对目前天然气管输企业发展过程中存在的输差控制问题,指出应建立天然气集输平衡与输差分析机制,并分析了其影响因素。在当前技术及管理现状的条件下对输差、输差定位基础工作、输差分析与输差控制等问题进行了探讨,提出了天然气输差控制理论和输差分析方法。

关键词：天然气管道输差控制分析

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自然人境外股权转让个人所得税反避税政策体系研究

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国际税收 2021年第6期 48-53页

作者：孔丹阳宋春辉国家税务总局北京市税务局国家税务总局北京市税务局第三税务分局

自然人境外股权转让具有交易行为隐蔽、避税手段复杂的特点,相关税收政策较为复杂且有待完善,是个人所得税和国际税收领域的焦点和难点所在。新修订的《中华人民共和国个人所得税法》在第八条中首次加入反避税条款,不仅开启了个人所得... 详细信息

自然人境外股权转让具有交易行为隐蔽、避税手段复杂的特点,相关税收政策较为复杂且有待完善,是个人所得税和国际税收领域的焦点和难点所在。新修订的《中华人民共和国个人所得税法》在第八条中首次加入反避税条款,不仅开启了个人所得税反避税的新篇章,也为进一步规范自然人境外股权转让反避税政策体系提供了契机。本文梳理了自然人境外股权转让涉及的个人所得税政策和征管要求,对该领域中的避税手段进行归纳和总结,从政策体系及征管机制等方面分析了自然人境外股权转让存在的问题,并在此基础上提出相应建议。

关键词：境外股权转让自然人个人所得税反避税

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腺苷酸激活蛋白酶介导糖尿病大鼠心肌线粒体自噬的机制与TSC2的关系研究

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重庆医科大学学报 2020年第3期45卷 338-342页

作者：宋春晖纪云西龚志刚江西师范大学生命科学学院南昌330022 浙江中医药大学附属浙江省中医院中医内科杭州310006 江西师范大学体育学院南昌330022

目的:探讨腺苷酸激活蛋白酶(AMP-activated protein kinase,AMPK)介导糖尿病大鼠心肌线粒体自噬的机制与结节性硬化复合物-2(tuberous sclerosis complex-2,TSC2)的关系。方法:成年雄性SD大鼠30只,随机分为对照组、模型组、模型+AMPK激... 详细信息

目的:探讨腺苷酸激活蛋白酶(AMP-activated protein kinase,AMPK)介导糖尿病大鼠心肌线粒体自噬的机制与结节性硬化复合物-2(tuberous sclerosis complex-2,TSC2)的关系。方法:成年雄性SD大鼠30只,随机分为对照组、模型组、模型+AMPK激动剂AICAR组(AICAR组)。采用腹腔注射链脲佐菌素建立大鼠糖尿病大鼠模型。AICAR组大鼠连续皮下注射AICAR 12周。采用超声诊断仪检测所有大鼠的心功能:左室舒张末期容积(left ventricular end-diastolic volume,LVEDV)、左室收缩末期容积(left ventricular end-systolic volume,LVESV)、左室射血分数(left ventricular ejection fraction,LVEF)、心率(heart rate,HR)、舒张早期血流峰值速度(peak velocity of early diastolic flow,E)、舒张晚期血流峰值速度(peak velocity of late diastolic flow,A)、E/A。取心肌组织,采用免疫共沉淀法检测AMPK和TSC2的结合水平;Western blot检测AMPK、TSC2、p-TSC2、LC3-Ⅱ、LC3-Ⅰ、Beclin-1和P62蛋白的表达水平。结果:AICAR组LVEDV、LVEF、E和E/A均高于模型组,而A低于模型组(P=0.004,P=0.001,P=0.000,P=0.011,P=0.027);AICAR组AMPK和TSC2蛋白表达水平高于模型组(0.71±0.06 vs.0.36±0.09,P=0.003;0.80±0.02 vs.0.40±0.05,P=0.017);AICAR组结合水平高于模型组(0.49±0.09 vs.0.23±0.03,P=0.002);AICAR组p-TSC2蛋白水平高于模型组(1.48±0.07 vs.0.92±0.07,P=0.029);AICAR组LC3-Ⅱ/LC3-Ⅰ、Beclin-1和P62均高于模型组(P<0.05)。结论:AMPK介导糖尿病大鼠心肌线粒体自噬的机制可能与其对TSC2的磷酸化作用有关。

关键词：糖尿病大鼠线粒体自噬腺苷酸激活蛋白酶结节性硬化复合物-2 心功能

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