Traits-based integration of multi-species inoculants facilitates shifts of indigenous soil bacterial community
作者单位:Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Core Facility Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Key Laboratory of Western China's Mineral Resources of Gansu Province School of Earth Sciences University of Lanzhou
会议名称:《中国土壤学会土壤环境专业委员会第二十次会议暨农田土壤污染与修复研讨会》
会议日期:2018年
学科分类:07[理学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 0713[理学-生态学]
关 键 词:Microbial co-inoculants Integration Bacterial community structure and function Soil properties Soil enzyme Cucumber
摘 要:Microbial co-inoculation is considered to be an innovative approach and had been applied worldwide. However, the underlying mechanisms of microbial co-inoculants constructions, especially the traits-based combination of distinctly different microbial species remains poorly understood. In this study, we constructed two microbial co-inoculants with the same three strains with emphasis on the microbial, soil and plant traits. Microbial co-inoculants 1(M1) were constructed according to soil fertility, microbial activity and cucumber nutrient requirement with a 2:1:2 ratio(Ensifer sp. NYM3, Acinetobacter sp. P16 and Flavobacterium sp. KYM3), while microbial co-inoculants 2(M2) were constructed according to soil fertility and cucumber nutrient requirement with a 1:10:1 ratio without considering the difference in the nutrient supply capability of microbial species. The results showed that M1 and M2 both obviously increased cucumber yields. The M1 had significant highest p H value, total nitrogen(TN) and Invertase activity(IA). The M2 had significant highest available phosphate(AP), NO-N, urea activity(UA) and Alkaline phosphatase activity(APA). Gammaproteobacteria, Acidobacteria, Nitrospirae and Armatimonadetes were significantly increased, while Actinobacteria and Firmicutes were significantly decreased by microbial co-inoculations(M1 and M2). The bacterial lineages enriched in M1 were Gammaproteobacteria and TM7. Acidobacteria, Bacteroidetes, and Deltaproteobacteria were enriched in M2. PCo A analysis showed that the bacterial communities were strongly separated by the different microbial inoculation treatments. The functional groups of intracellulararasites were highest in M1. The functional groups of phototrophy, photoautotrophy, nitrification, fermentation, cyanobacteria, oxygenichotoautotrophy, chitinolysis and animalarasitesrymbionts were highest in M2. Based on correlation analysis, it inferred that the M1 and M2 might promote cucumber yields by mediating bacterial