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GWAS unravels acid phosphatase ACP2 as a photosynthesis regulator under phosphate starvation conditions through modulating serine metabolism in rice

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Plant Communications 2024年第7期5卷 106-119页

作者： Sushuang liu Zhan Xu Jemaa Essemine Yanmin liu chundong liu Feixue Zhang Zubair Iqbal Mingnan Qu Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology Agricultural College of Yangzhou UniversityYangzhou 225009China Department of Life Sciences and Health Huzhou CollegeHuzhou 313000China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Institute of Crop Huzhou Academy of Agricultural SciencesHuzhou 313000China These authors contributed equally to this article

Inorganic phosphorus(Pi)deficiency significantly impacts plant growth,development,and photosynthetic *** study evaluated 206 rice accessions from a MiniCore population under both Pi-sufficient(Pi^(+))and Pi-starvation(Pi^(-))conditions in the field to assess photosynthetic phosphorus use efficiency(PPUE),defined as the ratio of A_(sat)^(Pi^(-))to A_(sat)^(Pi^(+)).A genome-wide association study and differential gene expression analyses identified an acid phosphatase gene(ACP2)that responds strongly to phosphate *** and knockout of ACP2 led to a 67%increase and 32%decrease in PPUE,respectively,compared with wild *** of an elite allele A,by substituting the v5 SNP G with A,resulted in an 18%increase in PPUE in gene-edited ACP2 rice *** phosphate-responsive gene PHR2 was found to transcriptionally activate ACP2 in parallel with PHR2 overexpression,resulting in an 11%increase in *** assays indicated that ACP2 primarily catalyzes the hydrolysis of phosphoethanolamine and *** addition,serine levels increased significantly in the ACP2^(vBG)overexpression line,along with a concomitant decrease in the expression of all nine genes involved in the photorespiratory *** of serine enhanced PPUE and reduced photorespiration rates in ACP2 mutants under Pi-starvation *** deduce that ACP2 plays a crucial role in promoting photosynthesis adaptation to Pi starvation by regulating serine metabolism in rice.

关键词： phosphate starvation GWAS photosynthetic adaption photosynthetic phosphate use efficiency genetic variation SNP rice

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In Situ Raman Monitoring of Trace Antibiotics in Different Harsh Water Environments

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Energy & Environmental Materials 2024年第1期7卷 284-293页

作者： chundong liu Fengcai Lei Maogang Gong Xiaoming Zhou Xiaofei Zhao Zhen Li Chao Zhang Baoyuan Man Jing Yu School of Physics and Electronics Institute of Materials and Clean EnergyShandong Normal UniversityJinan 250014China College of Chemistry Chemical Engineering and Materials ScienceInstitute of Biomedical SciencesShandong Normal UniversityJinan 250014China Department of Physics and Astronomy University of KansasLawrenceKansas 66045USA Shandong Provincial Hospital Shandong First Medical UniversityJinan 250021China

In situ surface-enhanced Raman scattering(SERS)is a widely used operando analytical technique,while facing numerous complex factors in applications under aqueous environment,such as low detection sensitivity,poor anti-interference capability,etc.,resulting in unreliable *** address these issues,herein a new hydrophobic SERS strategy has been *** comprehensively designing and researching a SERS-active structure of superhydrophobic ZnO/Ag nanowires,we demonstrate that hydrophobicity can not only draw analytes from water onto substrate,but also adjust"hottest spot"from the bottom of the nanowires to the *** a result,the structure can simultaneously concentrate the dispersed molecules in water and the enhanced electric field in structure into a same zone,while perfecting its own anti-interference *** underwater in situ analytical enhancement factor of this platform is as high as 1.67×10^(11),and the operando limited of detection for metronidazole(MNZ)reaches to 10^(-9)*** importantly,we also successfully generalized this structure to various real in situ detection scenarios,including on-site detection of MNZ in corrosive urine,real-time warning of wrong dose of MNZ during intravenous therapy,in situ monitoring of MNZ in flowing wastewater with particulate interference,etc.,demonstrating the great application potential of this hydrophobic *** work realizes a synergistic promotion for in situ SERS performance under aqueous environment,and also provides a novel view for improving other in situ analytical techniques.

关键词： antibiotic detection hydrophobic structure In situ Raman metronidazole wettability

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Chickpea C2H2-Type Zinc Finger Protein ZF2 is a Positive Regulator in Drought Response in Arabidopsis

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 577-590页

作者： Sushuang liu Yanmin liu chundong liu Xingwang Yu Hao Ma Department of Life Sciences and Health Huzhou CollegeHuzhou313000China State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing210095China Department of Crop and Soil Sciences North Carolina State UniversityRaleigh27695-7620USA Institute of Desert in the Arid Areas College of Grassland and Environment SciencesXinjiang Agricultural UniversityUrumqi830052China

Drought is a major abiotic stress limiting agricultural crops production *** our study,we isolated a novel C2H2-type zinc finger protein gene ZF2 from ***2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger *** expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized *** the yeast assay system,the full-length of ZF2 did not show transcriptional *** of ZF2 gene was enhanced by treatments of several abiotic stresses and *** promoter region of ZF2 contained multiple stress-and hormone-related *** of ZF2 in Arabidopsis significantly improved the root length and fresh weight at seedling stage and enhanced the survival rates and proline content under drought *** results indicated that ZF2 functioned as a positive regulator in drought response.

关键词： ZF2 chickpea drought tolerance transgenic

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Sulfoalkyl ether β-cyclodextrin derivatives synthesized by a single step method as pharmaceutical biomaterials

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Chinese Science Bulletin 2009年第18期54卷 3187-3199页

作者： SONG AiJing WANG JianHua liu chundong DENG LinHong Key Laboratory of Biorheological Science and Technology Ministry of Education Bioengineering College Chongqing UniversityChongqing 400030 China

We report a simple "one pot" single step method to synthesize sulfoalkyl ether β-cyclodextrin (β-CD) derivatives in aqueous solution, which avoids the limitations associated with commonly used organic synthesis of such derivatives including multi-step reactions, use of expensive, toxic, and often environmentally hazardous organic solvents and/or reagents. Using this method, we obtained sulfopropyl and sulfobutyl ethyl β-CDs. We characterized these two derivatives and evaluated their ability to enhance solubility of fluconazole, a commonly used antifungal drug with poor water solubility. Experimental results indicate that these derivatives are structurally analogous to some of the commercially available β-CD derivatives. More importantly, when they formed supramolecular inclusion complexes with fluconazole, they demonstrated similar, if not better, capacity to enhance fluconazole solubility compared with commercially available β-CD derivatives, suggesting that the simple "one pot" single step synthesis may provide an effective alternative approach to produce large quantity of β-CD derivatives as pharmaceutical biomaterials for medicinal applications.

关键词：环糊精衍生物合成方法生物材料医药超分子包合物醚衍生产品抗真菌药物

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泵控液压拉伸垫节能系统设计

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液压与气动 2024年第8期48卷 162-171页

作者：刘春东王恒张勇斌安会江何松董志奎河北建筑工程学院机械工程学院河北张家口075000 燕山大学机械工程学院河北秦皇岛066004 北自(北京)检测科技发展有限公司北京100032

泵控液压拉伸垫是压力机上常见的机械组成部分,针对其运行过程中电机再生制动产生的回馈能量被制动电阻消耗而造成的电功率浪费的问题,设计了共直流母线和电容储能的混合节能系统及调控策略。首先研究了混合节能系统的各个模块,通过数... 详细信息

泵控液压拉伸垫是压力机上常见的机械组成部分,针对其运行过程中电机再生制动产生的回馈能量被制动电阻消耗而造成的电功率浪费的问题,设计了共直流母线和电容储能的混合节能系统及调控策略。首先研究了混合节能系统的各个模块,通过数学建模和分析从理论推导上说明设计的可行性。其次建立泵控液压垫液压系统与节能系统仿真模型,在负载力为20 kN下进行仿真分析。最后搭建泵控液压拉伸垫实验台进行实验验证。仿真与实验结果表明,混合节能系统能起到很好的节能效果,系统的节能效率达到了41.9%。

关键词：液压垫共直流母线电容储能节能效率

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基于SolidWorks的陆空两用四旋翼搜救无人机设计与控制

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河北建筑工程学院学报 2024年第2期42卷 180-186页

作者：刘春东肖雷闫小军张东辉王亚飞河北建筑工程学院河北张家口075000

陆空两用搜救无人机应用前景广泛,可以在地面和空中进行有效搜索和救援行动。基于四旋翼飞行器设计了一种陆空两用四旋翼搜救无人机,完成了飞行平台和行走平台结构设计,编制了飞控的超声波定高系统和光流定位系统的算法程序,搭建了搜救... 详细信息

陆空两用搜救无人机应用前景广泛,可以在地面和空中进行有效搜索和救援行动。基于四旋翼飞行器设计了一种陆空两用四旋翼搜救无人机,完成了飞行平台和行走平台结构设计,编制了飞控的超声波定高系统和光流定位系统的算法程序,搭建了搜救系统。利用SolidWorks建立了三维建模,并对关键受力部件进行了有限元分析。对后续陆空两用四旋翼搜救无人机的研究具有重要的指导意义。

关键词：四旋翼搜救三维建模有限元分析

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基于波束形成的长短时记忆网络语音分离算法研究

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电子与信息学报 2022年第7期44卷 2531-2538页

作者：兰朝凤刘岩赵宏运刘春东哈尔滨理工大学测控技术与通信工程学院哈尔滨150080

在利用深度学习方式进行语音分离的领域,常用卷积神经网络(RNN)循环神经网络进行语音分离,但是该网络模型在分离过程中存在梯度下降问题,分离结果不理想。针对该问题,该文利用长短时记忆网络(LSTM)进行信号分离探索,弥补了RNN网络的不... 详细信息

在利用深度学习方式进行语音分离的领域,常用卷积神经网络(RNN)循环神经网络进行语音分离,但是该网络模型在分离过程中存在梯度下降问题,分离结果不理想。针对该问题,该文利用长短时记忆网络(LSTM)进行信号分离探索,弥补了RNN网络的不足。多路人声信号分离较为复杂,现阶段所使用的分离方式多是基于频谱映射方式,没有有效利用语音信号空间信息。针对此问题,该文结合波束形成算法和LSTM网络提出了一种波束形成LSTM算法,在TIMIT语音库中随机选取3个说话人的声音文件,利用超指向波束形成算法得到3个不同方向上的波束,提取每一波束中频谱幅度特征,并构建神经网络预测掩蔽值,得到待分离语音信号频谱并重构时域信号,进而实现语音分离。该算法充分利用了语音信号空间特征和信号频域特征。通过实验验证了不同方向语音分离效果,在60°方向该算法与IBM-LSTM网络相比,客观语音质量评估(PESQ)提高了0.59,短时客观可懂(STOI)指标提高了0.06,信噪比(SNR)提高了1.13 dB,另外两个方向上,实验结果同样证明了该算法较IBM-LSTM算法和RNN算法具有更好的分离性能。

关键词：语音分离超指向波束形成长短时记忆网络算法

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最低装量检查法改进的探讨

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中国药房 2006年第1期17卷 56-57页

作者：刘春栋天津市第二医院天津市300141

目的对原有最低装量检查法进行改进,使其更具有操作性,更符合一些特殊制剂的特殊要求。方法采用比重法、容量替代法、容量加和法,分别对溃疡糊、水晶膏、氧化锌搽剂进行最低装量检查。结果原先无法进行的最低装量检查有了可行的方法。... 详细信息

目的对原有最低装量检查法进行改进,使其更具有操作性,更符合一些特殊制剂的特殊要求。方法采用比重法、容量替代法、容量加和法,分别对溃疡糊、水晶膏、氧化锌搽剂进行最低装量检查。结果原先无法进行的最低装量检查有了可行的方法。结论改进后的方法可靠、操作性强。

关键词：最低装量检查比重法溃疡糊容量替代法水晶膏容量加和法氧化锌搽剂

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Traditional Chinese medicine Pien-Tze-Huang ameliorates LPS-induced sepsis through bile acid-mediated activation of TGR5-STAT3-A20 signalling

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Journal of Pharmaceutical Analysis 2024年第4期14卷 601-614页

作者： Bei Li Yong Zhang Xinyuan liu Ziyang Zhang Shuqing Zhuang Xiaoli Zhong Wenbo Chen Yilin Hong Pingli Mo Shuhai Lin Shicong Wang chundong Yu State Key Laboratory of Cellular Stress Biology School of Life SciencesXiamen UniversityXiamen361102China Fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development ZhangzhouFujian363000China Xiang'an Hospital of Xiamen University School of MedicineXiamen UniversityXiamen361005China Department of Cardiology Xiamen Key Laboratory of Cardiac ElectrophysiologyXiamen Institute of Cardiovascular DiseasesThe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen361003China

Pien Tze Huang(PZH),a class-1 nationally protected traditional Chinese medicine(TCM),has been used to treat liver diseases such as hepatitis;however,the effect of PZH on the progression of sepsis is ***,we reported that PZH attenuated lipopolysaccharide(LPS)-induced sepsis in mice and reduced LPS-induced production of proinflammatory cytokines in macrophages by inhibiting the activation of mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)***,PZH stimulated signal transducer and activator of transcription 3(STAT3)phosphorylation to induce the expression of A20,which could inhibit the activation of NF-κB and MAPK *** of the bile acid(BA)receptor G protein-coupled bile acid receptor 1(TGR5)in macrophages abolished the effects of PZH on STAT3 phosphorylation and A20 induction,as well as the LPS-induced inflammatory response,suggesting that BAs in PZH may mediate its anti-inflammatory effects by activating ***,deprivation of BAs in PZH by cholestyramine resin reduced the effects of PZH on the expression of phosphorylated-STAT3 and A20,the activation of NF-κB and MAPK signalling,and the production of proinflammatory cytokines,whereas the addition of BAs to cholestyramine resin-treated PZH partially restored the inhibitory effects on the production of proinflammatory ***,our study identifies BAs as the effective components in PZH that activate TGR5-STAT3-A20 signalling to ameliorate LPS-induced sepsis.

关键词： Pien Tze Huang Bile acids Sepsis Inflammation TGR5 STAT3-A20 signalling

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修饰性肽核酸的细胞转运

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生物工程学报 2016年第3期32卷 292-305页

作者：刘春冬王建华曾芳重庆大学生物工程学院重庆400044

肽核酸是人工合成的寡核苷酸类似物,以N-(2-氨乙基)甘氨酸结构单元替代DNA分子中的戊糖-磷酸结构。与天然核酸相比,肽核酸可以更高效地与DNA或RNA特异性杂交,在分子生物学和基因药物领域具有良好的应用前景。但是,肽核酸骨架呈电中性,... 详细信息

肽核酸是人工合成的寡核苷酸类似物,以N-(2-氨乙基)甘氨酸结构单元替代DNA分子中的戊糖-磷酸结构。与天然核酸相比,肽核酸可以更高效地与DNA或RNA特异性杂交,在分子生物学和基因药物领域具有良好的应用前景。但是,肽核酸骨架呈电中性,难以高效穿过细胞膜,这成为工程应用的最大障碍。为了改善肽核酸的细胞转运性能,对肽核酸进行化学修饰是近年来的研究热点。结合近十年来文献报道和本实验室的工作,对肽核酸的骨架修饰和配合物结合修饰两类增强细胞转运的修饰方法进行综述,并对修饰性肽核酸细胞转运研究中存在的问题以及未来的研究趋势及其应用提出了见解。

关键词：肽核酸修饰细胞转运

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