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Complement activation targeted inhibitor C2-FH ameliorates acetaminophen-induced liver injury in mice

World Journal of Hepatology 2024年第10期16卷 1188-1198页

作者： chun-mei li Tian Sun Mou-Jie Yang Zhi Yang Qing li Jia-lin Shi Chong Zhang Jun-Fei Jin Guangxi Key Laboratory of Molecular Medicine in liver Injury and Repair the Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Guangxi Health Commission Key Laboratory of Basic Research in Sphingolipid Metabolism Related Diseases the Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina China-United States lipids in Health and Disease Research Center Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Laboratory of Hepatobiliary and Pancreatic Surgery the Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina

BACKGROUND Complement activation is recognized as an important factor in the progression of liver damage caused by acetaminophen(APAP).However,the role of the complement inhibitor C2-FH in APAP-induced liver injury remains *** To explore C2-FH in protecting against APAP-induced liver injury by inhibiting complement *** A model of APAP-induced liver injury was used to study the protective effect of C2-FH on liver injury.C2-FH was administered through intraperitoneal injection 30 minutes after APAP *** detected the effects of C2-FH on liver function,inflammatory response and complement ***,RNA-sequencing(RNA-Seq)analysis was conducted to understand the mechanism through which C2-FH provides protection against APAP-induced liver *** C2-FH inhibited the increase in serum alanine aminotransferase activity,aspartate aminotransferase activity and lactate dehydrogenase,and reduced liver tissue necrosis caused by ***,it attenuated the inflammatory response and inhibited complement activation in APAP-induced liver ***-Seq analysis provided additional explanations for the protective role of C2-FH against APAP-induced liver *** C2-FH attenuates APAP-induced liver injury by inhibiting complement activation.

关键词： C2-FH Complement Complement activation Acetaminophen-induced liver injury Inflammation

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氧化石墨烯-DNA纳米探针用于三磷酸腺苷的检测与药物递送

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应用化学 2024年第1期41卷 118-127页

作者：张越梁蕊赵灿男李春梅西南大学药学院重庆400715

癌细胞内三磷酸腺苷(ATP)浓度异常与肿瘤发生发展过程密切相关,因此,快速、准确地检测细胞内外ATP水平具有重要意义。盐酸阿霉素(DOX)是一种广泛使用的抗癌药物,能嵌入DNA碱基对,并通过抑制DNA复制和转录诱导细胞凋亡。氧化石墨烯(Graph... 详细信息

癌细胞内三磷酸腺苷(ATP)浓度异常与肿瘤发生发展过程密切相关,因此,快速、准确地检测细胞内外ATP水平具有重要意义。盐酸阿霉素(DOX)是一种广泛使用的抗癌药物,能嵌入DNA碱基对,并通过抑制DNA复制和转录诱导细胞凋亡。氧化石墨烯(Graphene oxide, GO)由于具有毒性低、比表面积大和易功能化,可以有效、稳定地负载DNA纳米探针进入细胞等优点而被广泛应用。然而,复杂环境中的生物分子容易通过物理吸附竞争性结合到GO表面,导致假阳性信号。基于此,提出了一种新型的GO-DNA纳米探针,并将其应用于ATP的检测与抗癌药物DOX靶向递送。以ATP的核酸适配体与其互补链杂交形成双链DNA(dsDNA),并通过G-C碱基对负载DOX,利用互补链延伸的poly A序列吸附到GO表面构建了GO-dsDNA-DOX纳米探针,能极大程度地降低复杂环境中物理吸附引起的干扰,减少假阳性信号产生。ATP与适配体特异性结合会导致DOX释放,根据其荧光“off-on”实现ATP的定量分析,DOX荧光强度与ATP含量在0.08~8.0 mmol/L范围内呈现良好的线性关系,线性方程为IF=3.0897c+129.08,检测限(3σ/k)为0.059 mmol/L,且方法具有良好的选择性和抗干扰能力。细胞毒性及荧光成像结果表明,负载DOX的纳米探针在人乳腺癌细胞(MCF-7)内有明显的药物释放,显著诱导细胞凋亡。该研究建立了一种免修饰、简单和快速的ATP含量检测分析方法,并利用癌细胞内高浓度ATP实现靶向药物递送,降低了对正常细胞的毒副作用,为癌症的治疗提供了新思路。

关键词：三磷酸腺苷 DNA纳米探针氧化石墨烯荧光检测盐酸阿霉素药物递送

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Carbon dots prepared for fluorescence and chemiluminescence sensing

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Science China Chemistry 2019年第8期62卷 968-981页

作者： Meng-li liu Bin-Bin Chen chun-mei li Cheng-Zhi Huang Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China Chongqing Key Laboratory of Biomedical Analysis (Southwest University) Chongqing Science & Technology CommissionCollege of Pharmaceutical ScienceSouthwest UniversityChongqing 400715China

Carbon dots(CDs) have attracted considerable research interest in recent years due to their unique optical properties, chemical inertness, facile synthesis from a wide range of starting materials, and advantages over classic quantum dots and organic *** methods have been developed for preparing the CDs, including chemical oxidation, hydro/solvothermal method,electrochemical method, microwave-assisted synthesis, and direct carbonization method. Importantly, the superior electronic properties of CDs including efficient light harvesting and prominent photoinduced electron transfer have aroused considerable attention in fluorescence(FL) and chemiluminescence(CL) sensing field. In this review, we aim to demonstrate the recent progress of CDs in the synthesis, FL and CL sensing applications. This review gives new insights into how to use different synthetic methods to tune the structure of the CDs, with the major focus on FL and CL sensing.

关键词： carbon dots synthesis approach fluorescence sensing chemiluminescence sensing detection mechanism

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NRGA1, a Putative Mitochondrial Pyruvate Carrier, Mediates ABA Regulation of Guard Cell Ion Channels and Drought Stress Responses in Arabidopsis

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Molecular Plant 2014年第10期7卷 1508-1521页

作者： chun-Long li mei Wang Xiao-Yan Ma Wei Zhang Key Laboratory of Plant Cell Engineering and Germplasm Innovation Ministry of Education School of Life Science Shandong UniversityJinan 250100 China

Abscisic acid （ABA） regulates ion channel activity and stomatal movements in response to drought and other stresses. Here, we show that the Arabidopsis thaliana gene NRGA1 is a putative mitochondrial pyruvate carrier which negatively regulates ABA-induced guard cell signaling. NRGA1 transcript was abundant in the A. thaliana leaf and par- ticularly in the guard cells, and its product was directed to the mitochondria. The heterologous co-expression of NRGA1 and AtMPC1 in yeast complemented a loss-of-function mitochondrial pyruvate carrier （MPC） mutant. The nrgal loss-of- function mutant was very sensitive to the presence of ABA in the context of stomatal movements, and exhibited a height- ened tolerance to drought stress. Disruption of NRGA1 gene resulted in increased ABA inhibition of inward K＋ currents and ABA activation of slow anion currents in guard cells. The nrgal/NRGA1 functional complementation lines restored the mutant＇s phenotypes. Furthermore, transgenic lines of constitutively overexpressing NRGA1 showed opposite stomatal responses, reduced drought tolerance, and ABA sensitivity of guard cell inward K＋ channel inhibition and anion channel activation. Our findings highlight a putative role for the mitochondrial pyruvate carrier in guard cell ABA signaling in response to drought.

关键词： NRGAI mitochondrial pyruvate carrier ABA guard cell stomata inward K+ channel slow anion channel drought stress.

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Alloying and magnetic disordering effects on phase stability of Co_(2)YGa(Y=Cr,V,and Ni)alloys:A first-principles study

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Chinese Physics B 2022年第5期31卷 513-526页

作者： chun-mei li Shun-Jie Yang Jin-Ping Zhou College of Physics Science and Technology Shenyang Normal UniversityShenyang 110034China

The alloying and magnetic disordering effects on site occupation,elastic property,and phase stability of Co_(2)Y Ga(Y=Cr,V,and Ni)shape memory alloys are systematically investigated using the first-principles exact muffin-tin orbitals *** is shown that with the increasing magnetic disordering degree y,their tetragonal shear elastic constant C′(i.e.,(C_(11)-C_(12))/2)of the L2_(1) phase decreases whereas the elastic anisotropy A increases,and upon tetragonal distortions the cubic phase gets more and more ***_(2)Cr Ga and Co_(2)VGa alloys with y≥0.2 thus can show the martensitic transformation(MT)from L2_(1)to D0_(22)as well as Co_(2)Ni *** off-stoichiometric alloys,the site preference is controlled by both the alloying and magnetic *** the ferromagnetism state,the excessive Ga atoms always tend to take the Y sublattices,whereas the excessive Co atom favor the Y sites when Y=Cr,and the excessive Y atoms prefer the Co sites when Y=*** Ga-deficient Y=V alloys can also occur the MT at the ferromagnetism state by means of Co or V doping,and the MT temperature TMshould increase with their *** the corresponding ferromagnetism Y=Cr alloys,nevertheless,with Co or Cr substituting for Ga,the reentrant MT(RMT)from D0_(22)to L2_(1)is promoted and then TMfor the RMT should *** alloying effect on the MT of these alloys is finally well explained by means of the Jahn-Teller effect at the paramagnetic *** the ferromagnetism state,it may originate from the competition between the austenite and martensite about their strength of the covalent banding between Co and Ga as well as Y and Ga.

关键词： first-principles martensitic transformation elastic modulus magnetic ordering shape memory alloys

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An Efficient Catalyst for the Direct Synthesis of N-Phenylpyrrolidine

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Chinese Chemical Letters 2006年第12期17卷 1540-1542页

作者： chun mei li Zhi li MIAO Lei SHI Qi SUN Yu Hong HE Institute of Chemistry for Functionalized Materials Faculty of Chemistry and Chemical Engineering Liaoning Normal University Dalian 116029 liaoyuan Health School Liaoyuan 136200

N-Phenylpyrrolidine was efficiently synthesized over the mesoporous A1203 catalyst by the reaction of the aniline and 1,4-butylene-glycol at atmospheric pressure. The catalyst exhibited very high activity and selectivity. At the reaction temperature of 300~C, 1,4-butylene glycol conversion attained 100% and the selectivity of N-phenylpyrrolidine could exceed 98%.

关键词： Synthesis of N-phenylpyrrolidine mesoporous A1203 catalyst activity selectivity,aniline 1,4-butylene glycol.

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Deep-cryogenic-treatment-induced phase transformation in the Al-Zn-Mg-Cu alloy

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International Journal of Minerals,Metallurgy and Materials 2015年第1期22卷 68-77页

作者： chun-mei li Nan-pu Cheng Zhi-qian Chen Ning Guo Su-min Zeng School of Materials Science and Engineering Central South University School of Materials Science and Engineering Southwest University

An aluminum alloy （Al-Zn-Mg-Cu） subjected to deep cryogenic treatment （DCT） was systematically investigated. The results show that a DCT-induced phase transformation varies the microstructures and affects the mechanical properties of the Al alloy. Both Guinier-Preston （GP） zones and a metastableη′phase were observed by high-resolution transmission electron microscopy. The phenomenon of the second precipitation of the GP zones in samples subjected to DCT after being aged was observed. The viability of this phase transfor-mation was also demonstrated by first-principles calculations.

关键词： aluminum alloys cryogenic treatment phase transformation microstructure

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Proteomic analysis of seed germination under salt stress in soybeans

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第7期12卷 507-517页

作者： Xiao-yan XU Rui FAN Rui ZHENG chun-mei li De-yue YU National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China) Foundation Department Jiangsu Polytechnic College of Agriculture and Forestry Jurong 212400 China) College of life Sciences Ningxia University Yinchuan 750021 China)

Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline ***,the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic *** salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed *** both cultivars,the final germination percentage was not affected by salt,but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d,respectively,compared with *** response to salt stress,the abscisic acid content increased,and gibberellic acid (GA1+3) and isopentenyladenosine ***-3-acetic acid increased in Lee68,but remained unchanged in *** proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE),followed by Coomassie brilliant blue G-250 *** 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved,of which 18 protein spots showed changes in abundance as a result of salt stress in both *** matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins,the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully *** and 20S proteasome subunit β-6 were up-regulated in both *** 3-phosphate dehydrogenase,glutathione S-transferase (GST) 9,GST 10,and seed maturation protein PM36 were down-regulated in Lee68 by salt,but still remained at a certain ***,these proteins were present in lower levels in control N2899 and were up-regulated under salt *** results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination.

关键词： Proteomics Salt stress Seed germination Soybean

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AuNPs/graphene Hybrids-Based Enzyme-Free Plasmonic Immunoassay for Respiratory Syncytial Virus Detection

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Journal of Analysis and Testing 2021年第3期5卷 203-209页

作者： Lei Zhan chun-mei li Peng-Fei Gao Cheng-Zhi Huang Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationCollege of Pharmaceutical SciencesSouthwest UniversityChongqing 400715China

Accurate diagnosis of early infections caused by the viral pathogen is a major challenge because patients usually have nonspecific *** development of sensitive,reliable,robust,and rapid laboratory tools is still in high ***,we developed an extremely sensitive and affordable plasmonic immunoassay for respiratory syncytial virus(RSV)detection with the naked eye based on gold nanoparticles(AuNPs)/graphene hybrids combined with metal enhancement *** limit of detection was found to be 1.7 fg/mL,which was 100 times higher than that of the AuNPs-based *** ultrahigh sensitivity could be attributed to the large number of well-dispersed AuNPs deposited on the graphene surface,which had an extremely high relative surface *** signal amplification was achieved by catalytic enlargement of AuNPs with HAuCl_(4)-NH_(2)OH redox ***,the plasmonic immunoassay was successfully used to detect RSV in artificial serum samples,showing great potential in practical applications.

关键词： AuNPs/graphene hybrids Plasmonic immunoassay Signal amplification Respiratory syncytial virus

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Preparation and characterization of supramolecular gel suitable for fractured formations

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Petroleum Science 2023年第4期20卷 2324-2342页

作者： Jing-Bin Yang Jin-Sheng Sun Ying-Rui Bai Kai-He Lv Jian li mei-chun li Yue-Cheng Zhu School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongPR China CNPC Engineering Technology R&D Company limited Beijing102206PR China

The excellent mechanical properties of supramolecular gel could adapt to the complex reservoir environment and had broad application prospects in the field of oil and gas drilling and production *** this paper,a supramolecular gel based on hydrophobic association and hydrogen bonding was prepared by micellar copolymerization,which could be used to plug fractures and pores in *** gel was a gel network system with high performance characteristics formed by self-assembly of non-covalent bond *** rheological properties,mechanical mechanics,temperature resistance and swelling ability of supramolecular gel were *** results showed that the supramolecular gel had a dense three-dimensional network structure with open and interconnected pore structures,which could exhibit good rheological properties and strong viscoelastic recovery *** mechanical properties of the supramolecular gel were excellent,it had a tensile stress of 0.703 MPa and an elongation at break of 1803%.When the compressive strain was 96%,the compressive stress could reach 14.5 *** gel also showed good temperature resistance and swelling *** the aging temperature of 135℃,supramolecular gels still maintained good gel strength,and it only took 12 h to reach the equilibrium swelling ratio of 35.87 in 1%NaCl *** was also found that supramolecular gel in low concentration saline(1%NaCl solution)showed relatively faster swelling than high concentration saline(25%NaCl solution).The swelling process of the supramolecular gel was non-Fick diffusion(typeⅡ).This indicated that the organic/inorganic permeability network was well ***,the diffusion rate of small molecules could be guaranteed to be equal to the relaxation rate of large molecules before and after the phase transition *** addition to the diffusion of water molecules,the swelling process of the supramolecular gel was also affected by the relaxat

关键词： Supramolecular gel Non-covalent interaction Rheological mechanics properties Swelling kinetics Plugging ability

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