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3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities

Journal of Materials Science & Technology 2021年第4期63卷 9-17页

作者： Tzu-Cheng Sung Chao-Wen Heish Henry Hsin-chung Lee Jhe-Yu Hsu chun-ko wang Jia-Hua wang Yu-Ru Zhu Shih-Hsi Jen Shih-Tien Hsu Abdurahman HHirad Abdullah AAlarfaj Akon Higuchi School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityNo.270Xueyuan RoadWenzhouZhejiang325027China Department of Chemical and Materials Engineering National Central UniversityTaoyuan 32001Taiwan Department of Surgery Hsinchu Cathay General HospitalHsinchu 30060China Graduate Institute of Translational and Interdisciplinary Medicine National Central UniversityTaoyuan 32001Taiwan Department of Obstetrics and Gynecology Landseed HospitalTaoyuan 32405Taiwan Department of Internal Medicine Landseed HospitalTaoyuan 32405Taiwan Department of Botany and Microbiology King Saud UniversityRiyadh 11451Saudi Arabia Wenzhou Institute University of Chinse Academy of ScienceWenzhou 325001China Center for Emergent Matter Science Riken2-1 HirosawaWakoSaitama 351-0198Japan

Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and ***,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension *** this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing *** hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process ***,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to ***,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D *** reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing *** study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in *** discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.

关键词： culture alone stem

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Erratum to“3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities”[Journal of Materials Science&Technology 63(2021)9-17]

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Journal of Materials Science & Technology 2022年第16期111卷 307-307页

作者： Tzu-Cheng Sung Chao-Wen Heish Henry Hsin-chung Lee Jhe-Yu Hsu chun-ko wang Jia-Hua wang Yu-Ru Zhu Shih-His Jen Shih-Tien Hsu Abdurahman H.Hirad Abdullah A.Alarfaj a School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityNo.270Xueyuan RoadWenzhouZhejiang 325027China Department of Chemical and Materials Engineering National Central UniversityTaoyuanTaiwan 32001China Department of Surgery Hsinchu Cathay General Hospital30060China Graduate Institute of Translational and Interdisciplinary Medicine National Central UniversityTaoyuanTaiwan 32001China Department of Obstetrics and Gynecology Landseed HospitalTaoyuanTaiwan 32405China Department of Internal Medicine Landseed HospitalTaoyuanTaiwan 32405China Department of Botany and Microbiology King Saud UniversityRiyadh 11451Saudi Arabia Wenzhou Institute University of Chinese Academy of ScienceWenzhou 325001China

The affiliations of several authors were modified during the proofing stage at the request of corresponding author Akon Higuchi,without notifying the involved authors and the Editorial *** communication with all autho... 详细信息

关键词： parties agreed request

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Clinical values of Barcelona Clinic Liver Cancer subgroup and up-to-7 criteria in intermediate stage hepatocellular carcinoma with transcatheter arterial chemoembolization

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World Journal of Clinical Cases 2022年第21期10卷 7275-7284页

作者： Shou-Wu Lee Yen-chun Peng Han-chung Lien chung-wang ko chun-Fang Tung Chi-Sen Chang Division of Gastroenterology and Hepatology Department of Internal MedicineTaichung Veterans General HospitalTaichung 40705Taiwan Department of Internal Medicine Yang Ming Chiao Tung UniversityTaipei 112304Taiwan Department of Post-Baccalaureate Medicine College of MedicineChung Hsing UniversityTaichung 40227Taiwan Department of Internal Medicine Chung Shan Medical UniversityTaichung 40201Taiwan

BACKGROUND Transarterial chemoembolization(TACE)is a recommended treatment for patients with intermediate stage hepatocellular carcinoma(HCC)but with variable treatment *** To determine factors for predicting outcomes of TACE in patients with intermediate stage B *** Patients with Barcelona Clinic Liver Cancer(BCLC)stage B HCC who underwent TACE as the primary treatment were enrolled at Taichung Veterans General Hospital from January 2005 to December *** were assigned to either the objective responder(OR)group or the non-OR group according to mRECIST *** and radiological characteristics were compared between the 2 *** overall survival of enrolled subjects was *** In 128 enrolled patients,66(51.6%)were in the OR group and 62(48.4%)in the non-OR *** with the non-OR group,the OR group had a significantly smaller HCC size(6.55 cm vs 9.50 cm,P=0.001)and was within the up-to-7 criteria(50%vs 26.7%,P=0.001).After multivariable analyses,these significant associations still *** survival rate of all the subjects averaged 20.65±13.26 *** survival rate at 1-year was 64.8%,2-year was 46.9%,and 3-year was 31.2%.For those patients with OR to TACE,smaller tumor size and within up-to-7 criteria were associated with significantly better overall *** patients with subgroup B1 had the highest OR ratio(75%)and better overall survival(26.70±12.07 mo)after *** BCLC stage B HCC patients with smaller tumor size or within up-to-7 criteria had better survival outcomes to *** stage B subgroup is useful to predict refractoriness to TACE.

关键词： Hepatocellular carcinoma Objective response Overall survival Transcatheter arterial chemoembolization Barcelona Clinic Liver Cancer

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Sustainability-oriented prioritization of nuclear fuel cycle transitions in China:a holistic MCDM framework under uncertainties

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Nuclear Science and Techniques 2024年第9期35卷 196-212页

作者： Lei wang Ru-Xing Gao Hyo On Nam Hong Jang Won Il ko chun-Dong Zhang Guo-An Ye Wen-Heng Jing State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing 211816China School of Energy Science and Engineering Nanjing Tech UniversityNanjing 211816China korea Atomic Energy Research Institute 989-111 Daedeok-daeroYuseong-GuDaejeon 34057Republic of Korea China Institute of Atomic Energy P.O.Box 275-1Beijing 102413China

A sustainability-oriented assessment of the nuclear energy system can provide informative and convincing decision-making support for nuclear development strategies in *** our previous study,four authentic nuclear fuel cycle(NFC)transi-tion scenarios were proposed,featuring different development stages and exhibiting distinct environmental,economic,and technical ***,because of the multiple and often conflicting criteria embedded therein,determining the top-priority NFC alternative for a sustainability orientation remains *** address this issue,this study proposed a novel hybrid multi-criteria decision-making framework comprising fuzzy AHP,PROMETHEE GAIA,and ***,an improved fuzzy AHP weighting model was developed to determine criteria weights under uncertainty and investigate the influence of various weight aggregation and defuzzification ***,PROMETHEE GAIA was used to address conflicts among the criteria and prioritize alternatives on a visualized k-dimensional GAIA *** a result,the alternative for direct recycling PWR spent fuel in fast reactors is considered the most ***,a sensitivity analysis was conducted to examine the influence of criteria weight variation and validate the screening ***,using MOORA,some significant optimization ideas and valuable insights were provided to support decision-makers in shaping nuclear development strategies.

关键词： Nuclear energy systems Nuclear fuel cycle Fuzzy AHP PROMETHEE GAIA

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Influence of Teachers’Occupational Stress on Anxiety by Using Cross-Media Teaching Method

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International Journal of Mental Health Promotion 2022年第5期24卷 649-664页

作者： Hean Liu Young chun ko College of Science Hunan City UniversityYiyang413000China Department of Education Science Sehan UniversityChonnam58447Korea Department of Teaching Profession Sehan UniversityChonnam58447Korea

This paper is to study the conditions of teachers’occupational stress and anxiety by using cross-media teaching method,and reveals the inﬂuence relationship between *** this end,a questionnaire survey of 228 teachers using cross-media teaching method from 3 schools in Guangdong Province(China)was *** conclu-sions are as follows:Teachers who use cross-media teaching method have high levels of occupational stress and anxiety,lack of leadership and administrative support,overloaded work,state anxiety and trait anxiety are all at a high *** general characteristics differences,gender does not constitute a factor causing occupational stress and anxiety of the teachers using cross-media *** the increase in the use of cross-media teach-ing,teachers feel gradually increase of occupational stress and trait anxiety in more work tasks,and occupational stress and state anxiety shows ups and downs due to lack of school policy *** the relationship between occupational stress and anxiety,occupational stress and sub-variables without leadership and administrative sup-port,overloaded work,relationships with colleagues,and relationships with parents are all positively correlates with anxiety and have signiﬁcant positive ***,whether the inﬂuence of occupational stress sub-vari-able on anxiety,or the state anxiety and trait anxiety of the anxiety sub-variables,overloaded work and lack of leadership and administrative support have always been the key factors that cause ***,if the school or the relevant organization provides appropriate support and assistance to cross-media teaching,or appropri-ately reduce heavy tasks of teachers in cross-media teaching,so as to relieve occupational press and anxiety of the teachers,create good teaching quality,and promote the development of teaching technology.

关键词： Cross-media teaching teacher occupational stress anxiety

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TRAF2 regulates T cell immunity by maintaining a Tpl2-ERK survival signaling axis in effector and memory CD8 T cells

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Cellular & Molecular Immunology 2021年第9期18卷 2262-2274页

作者： Xiaoping Xie Lele Zhu Zuliang Jie Yanchuan Li Meidi Gu Xiaofei Zhou Hui wang Jae-Hoon Chang chun-Jung ko Xuhong Cheng Shao-Cong Sun Department of Immunology The University of Texas MD Anderson Cancer Center7455 Fannin StreetBox 902HoustonTX77030USA Jiangsu Key Laboratory of Immunity and Metabolism Department of Pathogenic Biology and ImmunologyXuzhou Medical University209 Tongshan RoadXuzhou221004JiangsuChina College of Pharmacy Yeungnam UniversityGyeongsan712-749Republic of Korea MD Anderson Cancer Center UT Health Graduate School of Biomedical Sciences HoustonTX77030USA

Generation and maintenance of antigen-specific effector and memory T cells are central events in immune responses against *** show that TNF receptor-associated factor 2(TRAF2)maintains a survival signaling axis in effector and memory CD8 T cells required for immune responses against *** signaling axis involves activation of Tpl2 and its downstream kinase ERK by NF-κB-inducing kinase(NIK)and degradation of the proapoptotic factor *** mediates Tpl2 activation by stimulating the phosphorylation and degradation of the Tpl2 inhibitor ***,while NIK is required for Tpl2-ERK signaling under normal conditions,uncontrolled NIK activation due to loss of its negative regulator,TRAF2,causes constitutive degradation of p105 and Tpl2,leading to severe defects in ERK activation and effector/memory CD8 T cell ***,TRAF2 controls a previously unappreciated signaling axis mediating effector/memory CD8 T cell survival and protective immunity.

关键词： T cell survival Effector and memory CD8 T cells Protective immunity Bacterial infection TRAF2 NIK Tpl2

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Dynamic magnetic resonance imaging features of cavernous hemangioma in the manubrium:A case report

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World Journal of Clinical Cases 2021年第17期9卷 4262-4267页

作者： Tsung-Tai Lin Hsian-He Hsu Shih-chun Lee Yi-Jen Peng Kai-Hsiung ko Department of Radiology National Defense Medical CenterTri-Service General HospitalTaipei 114Taiwan Department of Thoracic Surgery National Defense Medical CenterTri-Service General HospitalTaipei 114Taiwan Department of Pathology Tri-Service General HospitalNational Defense Medical CenterTaipei 114Taiwan

BACKGROUND Osseous hemangiomas,especially those located in the manubrium,are rare benign *** a review of the literature,only three case reports of sternal hemangioma were found.A precise diagnosis is difficult because of their nonspecific findings on computed tomography(CT)/magnetic resonance imaging(MRI).CASE SUMMARY An 88-year-old woman was suffering from a progressively enlarging mass in the *** CT images showed an osteolytic and expansile lesion with cortical *** malformation was suspected after CT-guided *** the dynamic MRI scans,the mass showed a bright signal on the T2-weighted image,peripheral nodular enhancement on the early-phase images and progressive centripetal fill-in on the delayed-phase *** hemangioma was suspected preoperatively based on the MRI features and finally confirmed by histopathologic *** This uncommon case demonstrates the possible characteristic features of manubrium cavernous hemangioma on dynamic MRI scans;knowledge about these features may prevent patients from developing catastrophic complications,such as rupture or internal hemorrhage,caused by biopsy or surgery.

关键词： Hemangioma Bone tumor Sternum Magnetic resonance imaging Osteolytic mass Case report

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Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars

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Protein & Cell 2012年第2期3卷 123-131页

作者： Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping ko chun-Hsiang Huang Feifei Ren chun-Chi Chen Yanhe Ma Rey-Ting Guo Yuanxia Sun Industrial Enzymes National Engineering Laboratory Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China Institute of Biological Chemistry Academia SinicaTaipei 115TaiwanChina CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in *** cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used *** this study,the crystal structure of the *** DPEase was *** enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active *** crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also *** the complex structures of *** DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site ***,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed *** results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis.

关键词： D-psicose 3-epimerase ketohexose complex structure

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The Potassium−Air Battery:Far from a Practical Reality?

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Accounts of Materials Research 2021年第7期2卷 515-525页

作者： Wanwan wang Yi-chun Lu Electrochemical Energy and Interfaces Laboratory Department of Mechanical and Automation EngineeringThe Chinese University of Hong KongShatinNew Territories 999077Hong Kong SARChina

CONSPECTUS:An energy storage system is the key bottleneck toward the widespread use of renewable energy and the development of electric vehicles(EVs).Alkali metal−oxygen batteries,which have higher gravimetric energy densities(3500−935 Wh kg−1)than conventional lithium−ion batteries(100−265 Wh kg−1),are considered to be one of the promising next-generation energy storage *** the past decade,Li−O2 batteries have been the center of the research effort owing to their highest energy ***,the poor reversibility,low round-trip efficiency,and limited cycle life originating from sluggish kinetic and serious parasitic chemistry induced by singlet oxygen hamper the development of Li−O2 *** the sluggish kinetics and severe parasitic reactions are closely related to the discharge product *** Li−O2 batteries,K−O2 batteries based on potassium superoxide offer an attractive theoretical energy density(935 Wh kg−1)with a significantly improved energy efficiency and lifetime compared to other alkali metal−O2 *** fast and reversible O2/ko2 single−electron reaction exhibits higher redox kinetics compared to the Li−O2 redox chemistries and removes the needs of catalysts or redox *** addition,the earth abundant K greatly alleviates the global shortage and uneven regional distribution of *** unique advantages of the K−O2 system make it a promising candidate for low-cost and large-scale energy ***,the development of a K−O2 battery is still in its early stages and its round-trip efficiency is still lower than that of lithium−ion *** improvement in energy efficiency and cycle life of the K−O2 batteries is crucial prior to practical *** present Account combines our efforts and other representative works on fundamental understandings and design strategies toward next-generation K−O2 *** are offered on oxygen electrode reversibility and stability,anode stabilization and alternat

关键词： battery Battery electrolyte

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Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways

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Chinese Journal of Natural Medicines 2018年第7期16卷 481-489页

作者： ZENG Qiang chun-Hay ko Wing-Sum Siu LI Kai-Kai chun-Wai Wong HAN Xiao-Qiang YANG Liu Clara Bik-San Lau HU Jiang-Miao Ping-chung Leung Institute of Chinese Medicine The Chinese University of Hong Kong State Key Laboratory of Phytochemistry and Plant Resources in West China The Chinese University of Hong Kong Shenzhen Research Institute The Chinese University of Hong Kong Kunming Institute of Botany Chinese Academy of Sciences

Dendrobii Caulis(DC), named ‘Shihu' in Chinese, is a precious herb in traditional Chinese medicine. It is widely used to nourish stomach, enhance body fluid production, tonify "Yin" and reduce heat. More than thirty Dendrobium species are used as folk medicine. Some compounds from DC exhibit inhibitory effects on macrophage inflammation. In the present study, we compared the anti-inflammatory effects among eight Dendrobium species. The results provided evidences to support Dendrobium as folk medicine, which exerted its medicinal function partially by its inhibitory effects on inflammation. To investigate the anti-inflammatory effect of Dendrobium species, mouse macrophage cell line RAW264.7 was activated by lipopolysaccharide. The nitric oxide(NO) level was measured using Griess reagent while the pro-inflammatory cytokines were tested by ELISA. The protein expressions of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2) and mitogen-activated protein kinases(MAPKs) phosphorylation were evaluated by Western blotting analysis. Among the eight Dendrobium species, both water extracts of D. thyrsiflorum ***(DTW) and D. chrysotoxum Lindl(DCHW) showed most significant inhibitory effects on NO production in a concentration-dependent manner. DTW also significantly reduced TNF-α, MCP-1, and IL-6 production. Further investigations showed that DTW suppressed iNOS and COX-2 expression as well as ERK and JNK phosphorylation, suggesting that the inhibitory effects of DTW on LPS-induced macrophage inflammation was through the suppression of MAPK pathways. In conclusion, D. thyrsiflorum *** was demonstrated to have potential to be used as alternative or adjuvant therapy for inflammation.

关键词： Dendrobium species Anti-inflammation Macrophage MAPK pathways

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