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Simple instruments facilitating achievement of transanal total mesorectal excision in male patients

World Journal of Gastroenterology 2017年第31期23卷 5798-5808页

作者： Chang Xu Hua-Yu Song Shao-Liang Han Shi-Chang Ni Hu-Xiang Zhang chun-gen xing Department of general Surgery the Second Affiliated Hospital of Soochow University Department of Colorectal Surgery the First Affiliated Hospital of Wenzhou Medical University Department of Pathology the First Affiliated Hospital of Wenzhou Medical University

AIM To assess the efficacy of a modified approach with transanal total mesorectal excision(ta TME) using simple customized instruments in male patients with low rectal *** A total of 115 male patients with low rectal cancer from December 2006 to August 2015 were retrospectively studied. All patients had a bulky tumor(tumor diameter ≥ 40 mm). Forty-one patients(group A) underwent a classical approach of transabdominal total mesorectal excision(TME) and transanal intersphincteric resection(ISR), and the other 74 patients(group B) underwent a modified approach with transabdominal TME,transanal ISR, and ta TME. Some simple instruments including modified retractors and an anal dilator with a papilionaceous fixture were used to perform ta TME. The operative time, quality of mesorectal excision, circumferential resection margin, local recurrence, and postoperative survival were *** All 115 patients had successful sphincter preservation. The operative time in group B(240 min, range: 160-330 min) was significantly shorter than that in group A(280 min, range: 200-360 min; P = 0.000). Co m pa r e d w it h g r o up A, m o r e c o m p le t e d is t a l mesorectum and total mesorectum were achieved in group B(100% vs 75.6%, P = 0.000; 90.5% vs 70.7%, P = 0.008, respectively). After 46.1 ± 25.6 mo followup, group B had a lower local recurrence rate and higher disease-free survival rate compared with group A, but these differences were not statistically significant(5.4% vs 14.6%, P = 0.093; 79.5% vs 65.1%, P = 0.130). CONCLUSION Retrograde ta TME with simple customized instruments can achieve high-quality TME, and it might be an effective and economical alternative for male patients with bulky tumors.

关键词： Rectal neoplasm Total mesorectal excision Transanal approach Intersphincteric resection Longterm outcome Local recurrence

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Effects of small interfering RNA inhibit Class Ⅰ phosphoinositide 3-kinase on human gastric cancer cells

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World Journal of Gastroenterology 2013年第11期19卷 1760-1769页

作者： Bao-Song Zhu Li-Yan Yu Kui Zhao Yong-You Wu Xiao-Li Cheng Yong Wu Feng-Yun Zhong Wei Gong Qiang Chen chun-gen xing Department of general Surgery The Second Affiliated HospitalSoochow University Department of Gastroenterology Qianfoshan HospitalShandong University

AIM: To investigate the effects of small interfering RNA (siRNA)-mediated inhibition of Class?I?phosphoinositide 3-kinase (Class?I?PI3K) signal transduction on the proliferation, apoptosis, and autophagy of gastric cancer SGC7901 and MGC803 cells.METHODS: We constructed the recombinant replication adenovirus PI3K(I)-RNA interference (RNAi)-green fluorescent protein (GFP) and control adenovirus NC-RNAi-GFP, and infected it into human gastric cancer cells. MTT assay was used to determine the growth rate of the gastric cancer cells. Activation of autophagy was monitored with monodansylcadaverine (MDC) staining after adenovirus PI3K(I)-RNAi-GFP and control adenovirus NC-RNAi-GFP treatment. Immunofluorescence staining was used to detect the expression of microtubule-associated protein 1 light chain 3 (LC3). Mitochondrial membrane potential was measured using the fluorescent probe JC-1. The expression of autophagy was monitored with MDC, LC3 staining, and transmission electron microscopy. Western blotting was used to detect p53, Beclin-1, Bcl-2, and LC3 protein expression in the culture supernatant.RESULTS: The viability of gastric cancer cells was inhibited after siRNA targeting to the Class?I?PI3K blocked Class?I?PI3K signal pathway. MTT assays revealed that, after SGC7901 cancer cells were treated with adenovirus PI3K(I)-RNAi-GFP, the rate of inhibition reached 27.48% ± 2.71% at 24 h, 41.92% ± 2.02% at 48 h, and 50.85% ± 0.91% at 72 h. After MGC803 cancer cells were treated with adenovirus PI3K(I)-RNAi-GFP, the rate of inhibition reached 24.39% ± 0.93% at 24 h, 47.00% ± 0.87% at 48 h, and 70.30% ± 0.86% at 72 h (P < 0.05 compared to control group). It was determined that when 50 MOI, the transfection efficiency was 95% ± 2.4%. Adenovirus PI3K(I)-RNAi-GFP (50 MOI) induced mitochondrial dysfunction and activated cell apoptosis in SGC7901 cells, and the results described here prove that RNAi of Class?I?PI3K induced apoptosis in SGC7901 cells. The results showed that a

关键词： Gastric cancer cells Class I phosphoinositide 3-kinase RNA interference Apoptosis Autophagy

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Expression of Embryonic Stem Cell Marker Oct-4 and Its Prognostic Significance in Rectal Adenocarcinoma

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Chinese Journal of Cancer Research 2010年第2期22卷 106-111页

作者： chun-gen xing Xue-guan Lu Yong-sheng Zhang Fang Zhou Xiao-ping Xu Department of general Surgery Second Affiliated Hospital of Soochow University Suzhou 215004 China Department of Radiation Oncology Second Affiliated Hospital of Soochow University Suzhou 215004 China Department of Pathology Second Affiliated Hospital of Soochow University Suzhou 215004 China

Objective： Recent evidence suggests that Oct-4 is highly expressed in several cancers, and its expression contributes to tumor growth. In this study, we investigated the level of Oct-4 expression in rectal adenocarcinoma, and evaluated the prognostic significance of Oct-4 expression in these cases. Methods： The immunohistochemical expression of Oct-4 was evaluated in 52 formalin-fixed paraffin-embedded postoperative rectal adenocarcinoma tissue samples. The impact of the immunoreactivity of Oct-4 in regard to clinical outcome was determined by Kaplan-Meier and log-rank. Results： The expression level of Oct-4 ranged from 0 to 18.5%. There was no significant association between Oct-4 expression and gender （P=0.772）, age （P=0.123）, clinical stage （P=0.391）, and histological grade （P=0.056）. The 3-year local recurrence-free rates with negative and positive expression of Oct-4 were 83.5% and 75.0%, respectively （P=0.583）. The 3-year metastasis-free rates with negative and positive expression of Oct-4 were 88.6% and 61.9%, respectively （P=0.035）. The 3-year overall survival rates with negative and positive expression of Oct-4 were 77.9% and 49.0%, respectively （P=0.037）. Conclusion： The results suggest that embryonic stem cell marker Oct-4 expression may have prognostic significance in patients with rectal adenocarcinoma. However, to confirm this more and larger studies are required.

关键词： Rectum Adenocarcinoma Oct-4 Stem ceil Prognosis

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Blocking NF-kB nuclear translocation leads to p53-related autophagy activation and cell apoptosis

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World Journal of Gastroenterology 2011年第4期17卷 478-487页

作者： Bao-Song Zhu chun-gen xing Fang Lin xiao-Qing Fan Kui Zhao Zheng-Hong Qin Department ofgeneral Surgery The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Laboratory of Aging and Nervous Diseases Soochow University School of Medicine Suzhou215123 Jiangsu Province China Department of general Surgery The Second Hospital Of Jiaxing City Jiaxing 314000 Jiangsu ProvinceChina

AIM: To investigate the anti-tumor effects of nuclear factor-κB (NF-κB) inhibitor SN50 and related mechanisms of SGC7901 human gastric carcinoma cells. METHODS: MTT assay was used to determine the cytotoxic effects of SN50 in gastric cancer cell line SGC7901. Hoechst 33258 staining was used to detect apoptosis morphological changes after SN50 treatment. Activation of autophagy was monitored with monodansylcadaverine (MDC) staining after SN50 *** staining was used to detect the expression of light chain 3 (LC3). Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Western blotting analysis were used to determine the expression of proteins involved in apoptosis and autophagy including p53, p53 upregulated modulator of apoptosis (PUMA), damage-regulated autophagy modulator (DRAM), LC3 and Beclin 1. We detected the effects of p53-mediated autophagy activation on the apoptosis of SGC7901 cells with the p53 inhibitor pifithrin-α. RESULTS: The viability of SGC7901 cells was inhibited after SN50 treatment. Inductions in the expression of apoptotic protein p53 and PUMA as well as autophagic protein DRAM, LC3 and Beclin 1 were detected with Western blotting analysis. SN50-treated cells exhibited punctuate microtubule-associated protein 1 LC3 in immunoreactivity and MDC-labeled vesicles increased after treatment of SN50 by MDC staining. Collapse of mitochondrial membrane potential Δψ were detected for 6 to 24 h after SN50 treatment. SN50-induced increases in PUMA, DRAM, LC3 and Beclin 1 and cell death were blocked by the p53 specific inhibitor pifithrin-α. CONCLUSION: The anti-tumor activity of NF-κB inhibitors is associated with p53-mediated activation of autophagy.

关键词： Nuclear factor-κB SN50 Autophagy P53 Cell apoptosis

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Effects of LY294002 on the invasiveness of human gastric cancer in vivo in nude mice

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World Journal of Gastroenterology 2009年第40期15卷 5044-5052页

作者： chun-gen xing Bao-Song Zhu Xiao-Qing Fan Hui-Hui Liu Xun Hou Kui Zhao Zheng-Hong Qin Department of general Surgery The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Department of general Surgery The Second Hospital of Jiaxing City Jiaxing 314000 Jiangsu Province China Department of Neurology The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Laboratory of Aging and Nervous Diseases Soochow University School of Medicine Suzhou 215123 Jiangsu Province China

AIM: To investigate the effects of class phosphatidy-linositol 3-kinase (PI3K) inhibitor LY294002 on the invasiveness and related mechanisms of implanted tumors of SGC7901 human gastric carcinoma cells in nude mice. METHODS: Nude mice were randomly divided into model control groups and LY294002 treatment groups. On days 5, 10 and 15 after treatment, the inhibitory rate of tumor growth, pathological changes in tumor specimens, expression levels of matrix metalloproteinase (MMP)-2, MMP-9, CD34 [representing microvessel density (MVD)] and vascular endothelial growth factor (VEGF), as well as apoptosis indexes in tumor samples were ***: In this study, we showed that treatingthe tumors with LY294002 could significantly inhibit carcinoma growth by 11.3%, 29.4% and 36.7%, after 5, 10 and 15 d, respectively, compared to the control group. Hematoxylin & eosin staining indicated that the rate of inhibition increased progressively (23.51% ± 3.11%, 43.20% ± 3.27% and 63.28% ± 2.10% at 5, 10 and 15 d, respectively) along with apoptosis. The expression of MMP-2 was also downregulated (from 71.4% ± 1.6% to 47.9% ± 0.7%, 31.9% ± 0.9% and 7.9% ± 0.7%). The same effects were observed in MMP-9 protein expression (from 49.4% ± 1.5% to 36.9% ± 0.4%, 23.5% ± 0.9% and 7.7% ± 0.6%), the mean MVD (from 51.2% ± 3.1% to 41.9% ± 1.5%, 30.9% ± 1.7% and 14.9% ± 0.8%), and the expression of VEGF (from 47.2% ± 3.1% to 25.9% ± 0.5%, 18.6% ± 1.2% and 5.1% ± 0.9%) by immunohistochemical ***: The classPI3K inhibitor LY294002could inhibit the invasiveness of gastric cancer cells by downregulating the expression of MMP-2, MMP-9, and VEGF, and reducing MVD.

关键词： Phosphatidylinositol 3-kinase LY294002 Gastric cancer Neoplasm invasiveness

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右侧结肠癌伴Sister Mary Joseph’s结节及腹股沟淋巴结转移——一例报告及文献综述(英文)

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癌症 2010年第2期29卷 255-258页

作者：吴永友邢春根蒋菊香吕孝东冯一中吴浩荣苏州大学附属第二医院普外科江苏苏州215004 苏州大学生物化学与分子生物学系江苏苏州215123 苏州大学附属第二医院病理科江苏苏州215004

Sister Mary Joseph’s结节(Sister Mary Joseph’s nodule,SMJN)是指腹腔内恶性肿瘤导致的脐部转移性结节,1949年由Hamilton Bailey命名以纪念***’s Hospital的护士Sister Joseph。SMJN并不多见,但个案报道屡屡见诸文献。这种脐部转移可以与腹腔内肿瘤同时出现或异时出现,是预后极差的表现。SMJN最常见原发肿瘤为胃癌及卵巢癌,结肠癌特别是右侧结肠癌引起的SMJN极其罕见。另外右侧结肠癌转移至腹股沟淋巴结未见文献报道。本文报道一例右侧结肠癌同时伴有SMJN及腹股沟淋巴结转移,并对相关文献进行综述。

关键词：结肠癌 Sister Mary Joseph’s结节脐部转移腹股沟淋巴结

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白细胞介素12基因的体外抗结肠癌效应

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苏州大学学报（医学版） 2004年第4期24卷 436-439页

作者：邢春根钱建新周丽英陈易人苏州大学附属第二医院江苏苏州215004 苏州大学附属第一医院江苏苏州215006

目的　构建共表达P35、P4 0二个亚基的逆转录病毒载体 (Rv mIL12 )、建立PA317 mIL12包装细胞系并评估其IL12的生物学活性及经PA317 mIL12作用后淋巴细胞对结肠癌细胞 (C2 6 )的体外杀伤作用。方法　将经PCR扩增mIL12 p35、p4 0获取的 ... 详细信息

目的　构建共表达P35、P4 0二个亚基的逆转录病毒载体 (Rv mIL12 )、建立PA317 mIL12包装细胞系并评估其IL12的生物学活性及经PA317 mIL12作用后淋巴细胞对结肠癌细胞 (C2 6 )的体外杀伤作用。方法　将经PCR扩增mIL12 p35、p4 0获取的 p35和 p4 0双亚基cDNA片段与经NotI及SalI酶切的逆转录病毒载体 (PL P35 P P4 0 SN)DNA在T4噬菌体DNA连接酶作用下进行粘端连接反应。然后将构建的 pGCXEXPN mIL12经电穿孔法转录单嗜性逆转录病毒包装细胞系 (PE5 0 1) ,再以其病毒上清加Polybrene感染PA317,筛选出病毒滴度最高的携有mIL12逆转录病毒载体的包装细胞系 :PA317 mIL12。采用脾淋巴细胞增殖法检测PA317 mIL12的生物学活性。另外 ,将PA317 mIL12、淋巴细胞以及人结肠癌细胞 (LOVO)按不同浓度比体外共同培养 ,采用MTT法检测经PA317 mIL12作用后的淋巴细胞对LOVO的杀伤率。结果　经筛选的携有mIL12逆转录病毒载体包装细胞系PA317 mIL12的上清mIL12浓度达 2 7ng/ ( 10 6cells·4 8h) ,其促脾淋巴细胞增殖的生物学活性也与mIL12标准品活性相近 ,经PA317 mIL12作用后的淋巴细胞体外杀伤试验结果则显示 :随着PA317 mIL12浓度的增高 ,其淋巴细胞对结肠癌细胞杀伤作用也逐渐增强 ,与对照组相比统计学有显著性差异

关键词：白细胞介素12基因结肠癌抗肿瘤效应生物学活性治疗

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Exploring the potential of extrachromosomal DNA as a novel oncogenic driver

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Science China Life Sciences 2025年第1期 144-157页

作者： Huanbo Zhu Longtao Huangfu Junbing Chen Jiafu Ji Xiaofang xing Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Division of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital & Institute Department of Gastrointestinal Cancer Center Peking University Cancer Hospital & Institute Department of Liver Surgery & Transplantation Liver Cancer InstituteZhongshan HospitalFudan University Key Laboratory of Carcinogenesis and Cancer Invasion Ministry of Education State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational ResearchDivision of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital & Institute

Extrachromosomal DNA(ecDNA) is a form of circular DNA mostly found in tumor cells. Unlike the typical chromosomal DNA, ecDNA is circular, self-replicating, and carries complete or partial gene fragments. Although ecDNA occurrence remains a rare event in cancer, recent studies have shown that oncogene amplification on ecDNA is widespread throughout many types of cancer, implying that ecDNA plays a central role in accelerating tumor evolution. ecDNA has also been associated with increased tumor mutation burden, chromosomal instability, and even tumor microenvironment remodeling. ecDNA may be crucial in influencing tumor heterogeneity, drug sensitivity,oncogenic senescence, and tumor immunogenicity, leading to a worsening prognosis for tumor patients. In this way, several clinical trials have been conducted to investigate the importance of ecDNA in clinical treatment. In this review, we summarize the biogenesis, characteristics, and current research methods of ecDNA, discuss the vital role of ecDNA-caused tumor heterogeneity in cancers, and highlight the potential role of ecDNA in cancer therapy.

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Insight Into the Separation-of-Variable Methods for the Closed-Form Solutions of Free Vibration of Rectangular Thin Plates

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 329-355页

作者： Yufeng xing Ye Yuan gen Li Institute of Solid Mechanics Beihang University(BUAA)Beijing100191China

The separation-of-variable(SOV)methods,such as the improved SOV method,the variational SOV method,and the extended SOV method,have been proposed by the present authors and coworkers to obtain the closed-form analytical solutions for free vibration and eigenbuckling of rectangular plates and circular cylindrical *** taking the free vibration of rectangular thin plates as an example,this work presents the theoretical framework of the SOV methods in an instructive way,and the bisection–based solution procedures for a group of nonlinear eigenvalue ***,the explicit equations of nodal lines of the SOV methods are presented,and the relations of nodal line patterns and frequency orders are *** is concluded that the highly accurate SOV methods have the same accuracy for all frequencies,the mode shapes about repeated frequencies can also be precisely captured,and the SOV methods do not have the problem of missing roots as well.

关键词： Separation-of-variable method Rayleigh quotient nodal line eigenvalue equation bisection method

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Surface microstructure control of microalloyed steel during slab casting

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Journal of Iron and Steel Research International 2017年第8期24卷 803-810页

作者： Li-jun Xu Shu-lan Zhang chun-gen Qiu Sheng-tao Qiu xing-zhong Zhang Central Iron and Steel Research Institute Beijing 100081China

Lots of work has been done to investigate slab surface microstructure evolution during continuous casting in order to improve hot ductility and avoid transverse *** slab surface microstructure after continuous casting was characterized by optical microscopy,and the precipitation behavior was investigated by transmission electron *** the same time,the mechanical properties of the slabs were measured using a Gleeble 1500 Dthermal simulator and the transformation temperatures were examined by means of a thermal *** experimental results show that homogeneous microstructure without film-like ferrites and chain-like precipitates at grain boundary can be obtained through surface intensive cooling and transverse cracks do not occur on the slab *** the experimental steel,fine ferrite can form at slab surface when the water flow rate is larger than 1560L/min at vertical *** the distance to surface increases,microstructure turned to ferrite and ***,nano-size carbonitrides precipitated in the ferrite grain and the size was larger at the junction of the *** mechanical experiment results show that the hot ductility of the sample deformed at 650°C was better than that of the sample deformed at 750°*** reason is that filmlike ferrite formed at the grain boundary in the sample deformed at 750°***,the slab must be cooled quickly below Ar3 to prevent the occurrence of film-like ferrite and transverse cracks on the slab surface during casting.

关键词： Microalloyed steel Surface intensive cooling Ferrite Carbonitride Crack

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