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Cloning of Salt Tolerance-Related cDNAs from the Mangrove Plant Sesuvium portulacastrum L.

Journal of Integrative Plant Biology 2006年第8期48卷 952-957页

作者： Hui-Cai Zeng Liu-Hong Deng chun-fa zhang State Key Laboratory of Tropical Crop Biotechnology Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Science （ CATAS） Kaikou 571101 China Environmental and Plant Protection Institute CATAS Danzhou 571737 China

In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant （Sesuvium portulacastrum L.）, a subtracted cDNA library was constructed via suppressive subtractive hybridization （SSH）, in which the poly（A）＋RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly（A）＋RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant （LEA） protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.

关键词： mangrove salt tolerance-related cDNAs Sesuvium portulacastrum suppressive subtractive hybridization.

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Light-controlled protein imprinted nanospheres with variable recognition specificity

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Chinese Chemical Letters 2025年第2期36卷 173-178页

作者： Mingqi Wang Shixin fa Jiate Yu Guoxian zhang Yi Yan Qing Liu Qiuyu zhang Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology School of Chemistry and Chemical Engineering Northwestern Polytechnical University

This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA) and lysozyme(Lyz) under different UV light through a gradient dual crosslinked imprinting strategy(i.e., covalent crosslinking and dynamic reversible crosslinking). The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA, and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA. Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA. Moreover, the N-isopropyl acrylamide(NIPAM) and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively. Therefore, the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H. As a result, the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light. This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.

关键词： Dynamically reversible crosslinking Molecularly imprinting Protein recognition Stimulus-response Variable specificity

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Nickel extraction from nickel laterites: Processes, resources, environment and cost

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China Geology 2025年第1期8卷 187-213页

作者： Zhen-fang zhang Wei-bo zhang Zhen-guo zhang Xiu-fa Chen Development Research Center of China Geological Survey Ministry of Natural ResourcesBeijing 100037China Inner Mongolia University of Technology Hohhot 010062China

With the development of the new energy industry and the depletion of nickel sulfide ore resources,laterite nickel ore has become the main source of primary nickel,and nickel for power batteries has become a new growth point in *** paper systematically summarizes the processes,parameters,products,recovery rates,environmental indicators,costs,advantages,disadvantages and the latest research progress of mainstream nickel extraction processes from laterite nickel *** also provides a comparative analysis of the environmental impact and economic efficiency of different nickel extraction *** is found that the current nickel extraction processes from laterite nickel ore globally for commercial production mainly include the RKEF process for producing ferronickel and the HPAL process for producing intermediate *** former accounts for about 80%of laterite nickel ore *** to each other,the investment cost per ton of nickel metal production capacity for the RKEF is about 43000$,with an operational cost of about 16000$per ton of nickel metal and a total nickel recovery rate of 77%–90%.Its products are mainly used in stainless *** the HPAL process,the investment cost per ton of nickel metal production capacity is about 56000$,with an operational cost of about 15000$per ton of nickel metal and a total nickel recovery rate of 83%–90%.Its products are mainly used in power *** significant differences between the two lies in energy consumption and carbon emissions,with the RKEF being 2.18 and 2.37 times that of the HPAL,*** the use of clean energy can greatly reduce the operational cost and environmental impact of RKEF,if RKEF is converted to producing high Ni matte,its economic and environmental performance still cannot match that of the HPAL and oxygen-enriched side-blown ***,it can be inferred that with the increasing demand for nickel in power batteries,HPAL and oxygen-enriched side

关键词： Laterite nickel ore Limonite Saprolite Nickel extraction Pyrometallurgy Power batteries Hydrometallurgy Cost Environmental impact Life cycle assessment Comprehensive utilization Mineral exploration engineering

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Phase equilibria of slag systems“FeO”−SiO_(2)−CaO−Al_(2)O_(3)and“FeO”−SiO_(2)−CaO−MgO at 1200℃and p(O_(2))of 10^(−7)kPa

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Transactions of Nonferrous Metals Society of China 2025年第1期35卷 338-348页

作者： Sui XIE Qin-meng WANG Xue-yi GUO chun-fa LIAO Bao-jun ZHAO faculty of Materials Metallurgy and ChemistryJiangxi University of Science and TechnologyGanzhou 341000China National Rare Earth Functional Material Innovation Center Ganzhou 341000China School of Metallurgy and Environment Central South UniversityChangsha 410083China Sustainable Minerals Institute The University of QueenslandBrisbane 4072Australia

High-temperature experiments were carried out for the slag systems of“FeO”−SiO_(2)−CaO−Al_(2)O_(3)and“FeO”−SiO_(2)−CaO−MgO at 1200℃and p(O_(2))of 10^(−7)*** equilibrated samples were quenched,and the phase compositions were measured by electron probe microanalysis(EPMA).A series of pseudo-ternary and pseudo-binary phase diagrams are constructed to demonstrate their applications in copper smelting process and evaluation of the thermodynamic *** and tridymite are identified to be the major primary phases in the composition range related to the copper smelting *** is found that the operating window of the smelting slag is primarily determined by w_(Fe)/w_(SiO_(2))ratio in the *** MgO and Al_(2)O_(3)in the slag reduce the operating window which requires extra fluxing agent to keep the slag to be fully *** spinel solid solutions cause inaccurate predictions of the current thermodynamic database.

关键词： phase equilibrium “FeO”−SiO_(2)−CaO−Al_(2)O_(3)slag system “FeO”−SiO_(2)−CaO−MgO slag system oxygen partial pressure copper smelting slag factSage

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Artificial self-powered and self-healable neuromorphic vision skin utilizing silver nanoparticle-doped ionogel photosynaptic heterostructure

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Journal of Semiconductors 2025年第1期46卷 205-213页

作者： Xinkai Qian fa zhang Xiujuan Li Junyue Li Hongchao Sun Qiye Wang Chaoran Huang Zhenyu zhang Zhe Zhou Juqing Liu Key Laboratory of Flexible Electronics(KLoFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816China

Artificial skin should embody a softly functional film that is capable of self-powering,healing and sensing with neuromorphic ***,the pursuit of a bionic skin that combines high flexibility,self-healability,and zero-powered photosynaptic functionality remains *** this study,we report a self-powered and self-healable neuromorphic vision skin,featuring silver nanoparticle-doped ionogel heterostructure as *** localized surface plasmon resonance induced by light in the nanoparticles triggers temperature fluctuations within the heterojunction,facilitating ion migration for visual sensing with synaptic *** abundant reversible hydrogen bonds in the ionogel endow the skin with remarkable mechanical flexibility and self-healing *** assembled a neuromorphic visual skin equipped with a 5×5 photosynapse array,capable of sensing and memorizing diverse light patterns.

关键词： neuromorphic vision skin ionogel heterojuction lspr photosynapse

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Propagating subduction initiation of the Caroline Plate at the Mussau Trench

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Science China Earth Sciences 2025年第1期68卷 253-269页

作者： chun-Feng LI Yongkang DENG Tao WU Dongdong DONG Guoliang zhang Jiazheng zhang Yutao LIU Yaqing LI Fugui TANG Lulu zhang Department of Marine Sciences Zhejiang UniversityZhoushan316021China Laboratory of Marine Mineral Resources Laoshan LaboratoryQingdao266237China Key Lab of Marine Geology and Environment Institute of OceanologyChinese Academy of SciencesQingdao266071China Key Lab of Ocean and Marginal Sea Geology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou510301China Science&Technology on Integrated Information System Lab Institute of SoftwareChinese Academy of SciencesBeijing100190China

New seismic imaging from the Mussau Trench confirms that,under horizontal compression on an oceanic plate boundary,it is within the young and weak oceanic plate that a trench will develop,and it is the young plate,not so long after its birth,that starts to subduct toward the old *** Mesozoic Pacific Plate cannot easily bend,under the joint horizontal compression and vertical gravity pull,even with a pre-existing weak zone in place and with a large age offset across the plate *** bending of the Caroline Plate near the northern end of the Mussau Trench started only after a long-time span of trench migration,and/or after a prolonged transition from initial compression to trench *** contrasting trench deformation and sedimentary accretion between two parallel seismic sections support an evident migration of underthrusting or significant longitudinal heterogeneity along the *** swift transition from underthrusting to non-thrusting can be explained by a counter-clockwise rotation of the Caroline *** miniature accretionary wedges develop even before the subduction initiates imply strong compression and uplifting before the underthrusting can *** of the Caroline Plate have been halted as the Mussau Trench is seismically rather quiet and the Lyra Trough to the east shows relaxational extension.

关键词： Mussau Trench Subduction initiation Seismic reflection Caroline Plate

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Transfer RNA-derived small RNA serves as potential non-invasive diagnostic marker and a novel therapeutic target for acute pancreatitis

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World Journal of Gastroenterology 2025年第1期31卷 152-156页

作者： Jing zhang chun-Lin Ou Department of Immunology School of Basic MedicineCentral South UniversityChangsha 410000Hunan ProvinceChina Department of Pathology Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina

Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational *** are not only degradation fragments but also have biological functions,including those in immune inflammation,metabolic disorders,and cell *** dysregulation is closely associated with multiple diseases,including various cancers and acute pancreatitis(AP).AP is a common gastrointestinal disease,and its incidence increases *** development is associated with tsRNAs,which regulate cell injury and induce inflammation,especially pyroptosis and ***,serum tRF36 has the potential to serve as a non-invasive diagnostic biomarker and leads to pancreatic acinar cell ferroptosis causing inflammation to promote *** show the characteristics of tsRNAs and their diagnostic value and function in AP,and discuss the potential opportunities and challenges of using tsRNAs in clinical applications and research.

关键词： Transfer RNA derived small RNAs Acute pancreatitis Liquid biopsy Biomarker Cell pyroptosis Ferroptosis

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Elucidating Secondary Organic Aerosol Processes through High-Resolution Aerosol Mass Spectrometry in Beijing

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Advances in Atmospheric Sciences 2025年第4期42卷 670-681页

作者： Weiqi XU chun CHEN Zhijie LI Zhiqiang zhang Yu zhang Zifa WANG Yele SUN Key Laboratory of Atmospheric Environment and Extreme Meteorology Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China China Meteorological Administration Training Centre Beijing 100081China

Exploring secondary organic aerosol(SOA)processes is crucial for understanding climate and air pollution in *** study introduces a new method using positive matrix factorization(PMF)to investigate the SOA process by integrating the OA and associated ions previously misidentified as inorganic aerosol in high-resolution aerosol mass spectrometry *** mass spectra and time series of primary OA(POA)and less oxidized oxygenated OA(OOA)identified by this new method resembled those resolved by traditional ***,more oxidized OOA(MO-OOA)identified by traditional PMF can be further subdivided into multiple OA factors,including nitrogen-enriched OA(ON-OA)and sulfur-enriched OA(OS-OA)in summer,and ON-OA,OS-OA,and OOA in *** findings highlight the significant role of photochemical processes in the formation of OS-OA compared to *** compositions of reconstructed MO-OOA varied under different Ox(=O_(3)+NO_(2))and relative humidity conditions,emphasizing the limitations of using a constant mass *** biomass burning OA(BBOA)and coal combustion OA(CCOA),previously misattributed as POA,contributed 9.2%(0.43μg m^(−3))and 7.0%(0.33μg m^(−3))to SOA,*** BBOA was more prone to forming OS-OA,whereas ON-OA showed higher correlations with aged CCOA,indicating distinct molecular compositions of SOA from different aged POA *** to aged BBOA,aged CCOA was more subject to conversion during aqueous phase *** results suggest that the variations in mass spectra and compositions need to be considered when simulating SOA processes.

关键词： secondary organic aerosol positive matrix factorization aerosol mass spectrometry nitrogen-enriched organic aerosol sulfur-enriched organic aerosol

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Deciphering Water Oxidation Catalysts:The Dominant Role of Surface Chemistry over Reconstruction Degree in Activity Promotion

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Nano-Micro Letters 2025年第3期17卷 340-354页

作者： Li An Jianyi Li Yuanmiao Sun Jiamin Zhu Justin Zhu Yeow Seow Hong zhang Nan zhang Pinxian Xi Zhichuan J.Xu chun‑Hua Yan State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceFrontiers Science Center for Rare IsotopesCollege of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000People’s Republic of China School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore faculty of Materials Science and Energy Engineering Institute of Technology for Carbon NeutralityShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055People’s Republic of China Key Laboratory of Electromagnetic Materials and Devices National Center for International Research On Photoelectric and Energy MaterialsSchool of Materials and EnergyElectron Microscopy CenterYunnan UniversityKunming 650091People’s Republic of China State Key Laboratory of Baiyunobo Rare Earth Resource Researches and Comprehensive Utilization Baotou Research Institute of Rare EarthsBaotou 014030People’s Republic of China Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsPKU‑HKU Joint Laboratory in Rare Earth Materials and Bioinorganic ChemistryPeking UniversityBeijing 100871People’s Republic of China

Water splitting hinges crucially on the availability of electrocatalysts for the oxygen evolution *** surface reconstruction has been widely observed in perovskite catalysts,and the reconstruction degree has been often correlated with the activity ***,a systematic study on the roles of Fe substitution in activation of perovskite LaNiO_(3)is *** substituting Fe content influences both current change tendency and surface reconstruction ***_(0.9)Fe_(0.1)O_(3)is found exhibiting a volcano-peak intrinsic activity in both pristine and reconstructed among all substituted perovskites in the LaNi_(1-x)Fe_(x)O_(3)(x=0.00,0.10,0.25,0.50,0.75,1.00)*** reconstructed LaNi_(0.9)Fe_(0.1)O_(3)shows a higher intrinsic activity than most reported NiFe-based ***,density functional theory calculations reveal that Fe substitution can lower the O 2p level,which thus stabilize lattice oxygen in LaNi0.9Fe0.1O3 and ensure its long-term ***,it is vital interesting that activity of the reconstructed catalysts relied more on the surface chemistry rather than the reconstruction *** effect of Fe on the degree of surface reconstruction of the perovskite is decoupled from that on its activity enhancement after surface *** finding showcases the importance to customize the surface chemistry of reconstructed catalysts for water oxidation.

关键词： Oxygen evolution reaction Perovskite oxides Doping Activation and reconstruction

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Effect of graphene addition on activation and kinetic properties of La-Mg-Ni-based hydrogen storage alloys

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Journal of Iron and Steel Research International 2025年第1期32卷 227-238页

作者： Dian-chen Feng chun-ling Zheng Zhi-yuan Zhao Dong-sheng Zhou Hui-ping Ren Yang-huan zhang School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou014010Inner MongoliaChina Collaborative Innovation Center of Integrated Exploitation of Bayan Obo Multi-Metal Resources Inner Mongolia University of Science and TechnologyBaotou014010Inner MongoliaChina Department of Functional Material Research China Iron and Steel Research Institute GroupBeijing100081China

The La_(1.7)Pr_(0.3)Mg_(16)Ni hydrogen storage alloy was prepared by medium-frequency induction melting,and then the composite hydrogen storage alloy powder of La_(1.7)Pr_(0.3)Mg_(16)Ni+x wt.%(x=0,2,4,and 6)graphene was prepared by ball milling for 10 *** effect of the addition of graphene on the activation and hydrogen de/absorption properties of La_(1.7)Pr_(0.3)Mg_(16)Ni alloy was *** result demonstrated that these composite alloys were composed of La_(2)Mg_(17),La_(2)Ni_(3),and Mg_(2)Ni *** saturated hydrogen absorption,it was composed of LaH_(3),Mg_(2)NiH_(4),and MgH_(2)phases,while during the dehydrogenation process,it was composed of LaH_(3),Mg,and Mg_(2)Ni *** addition of graphene can help get a more homogeneous granule after ball milling and accelerate the first activation of dehydrogenation/hydrogen *** hydrogen release activation energy of the alloys first decreases and then increases as the graphene content increases from x=0 wt.%to x=6 wt.%.The minimum activation energy of the composite hydrogen storage alloy is 51.22 kJ mol^(-1) when x=4 wt.%.

关键词： La-Mg-Ni alloy Graphene Mechanical ball milling Activation property Dynamic performance

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