Light-controlled protein imprinted nanospheres with variable recognition specificity

作     者：Mingqi Wang Shixin Fa Jiate Yu Guoxian Zhang Yi Yan Qing Liu Qiuyu Zhang 

作者机构：Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology School of Chemistry and Chemical Engineering Northwestern Polytechnical University 

出 版 物：《Chinese Chemical Letters》 (中国化学快报(英文版))

年 卷 期：2025年第36卷第2期

页      面：173-178页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：financial support from the National Natural Science Foundation of China (No. 22275148) National Key R&D Program of China (No. 2018YFB1900201) for Qiuyu Zhang the National Natural Science Foundation of China (No. 22271232) Fundamental Research Funds for the Central Universities (No. D5000230114) for Shixin Fa the Fundamental Research Funds for the Central Universities (No. D5000220339) for Qing Liu 

主　　题：Dynamically reversible crosslinking Molecularly imprinting Protein recognition Stimulus-response Variable specificity 

摘      要：This work develops a protein imprinted nanosphere with varied recognition specificity for bovine serum albumin(BSA) and lysozyme(Lyz) under different UV light through a gradient dual crosslinked imprinting strategy(i.e., covalent crosslinking and dynamic reversible crosslinking). The imprinting cavities are initially constructed using irreversible covalent crosslinking to specifically recognize BSA, and then the coumarin residues in the imprinting cavities are crosslinked under 365 nm UV light to further imprint Lyz,because Lyz has smaller size than BSA. Since the photo-crosslinking of coumarin is a reversible reaction,the imprinting cavities of Lyz can be de-crosslinked under 254 nm UV light and restore the imprinting cavities of BSA. Moreover, the N-isopropyl acrylamide(NIPAM) and pyrrolidine residues copolymerized in the polymeric surface of the nanospheres are temperature-and p H-responsive respectively. Therefore, the protein rebinding and release behaviors of the nanospheres are controlled by external temperature and p H. As a result, the materials can selectively separate BSA from real bovine whole blood and Lyz from egg white under different UV light. This study may provide a new strategy for construction of protein imprinted materials with tunable specificity for different proteins.