检索结果-南通市图书馆

Programmed nanoparticle-loaded microparticles for effective antigen/adjuvant delivery

Particuology 2022年第1期20卷 77-89页

作者： Rong Xu Ying Dong Yajing zhang Xiaoli Wang chuangnian zhang Yanjun Jiang Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Sciences&1 Peking Union Medical CollegeTianjin 300192China School of Chemical Engineering and Technology.Hebei University of Technology.Tianjin.300130 China NHC Key Laboratory of Hormones and Development Tianjin Key Laboratory of Metabolic DiseasesChu Hsien-I Memorial Hospital&Tianjin Institute of EndocrinologyTianjin Medical UniversityTianjin 300134China

A microscale vaccine containing SiO_(2)nanoparticles loaded in CaC〇3 microparticles was constructed using the co-precipitation *** antigen ovalbumin(OVA)was covalently conjugated with SiO_(2)nanoparticles,and these nanoparticles and CpG were co-encapsulated into CaCO_(3)microparticles,generating a vaccine with a size of approximately 5.2μ*** electron microscopy(SEM),energy-dispersive X-ray(EDX),elemental mapping,and Fourier transform infrared(FTIR)analyses confirmed the successful preparation of the microscale vaccine;the vaccine had good storage stability without sustained antigen release,and negligible cytotoxicity to dendritic cells(DCs)and *** to SiO_(2)nanoparticles,the microscale vaccine can significantly improve antigen/adjuvant *** internalized the entire microscale vaccine into lysosomes via macropinocytosis,and an increase in antigen endo/lysosomal escape was observed by confocal User scanning microscopy(CLSM).Specifically,DCs pulsed with the vaccine were fully mature,expressing high levels of costimulatory molecules(CD40,CD80,and CD86),MHCⅡ,and MHCⅠand secreting high levels of proinflammatory cytokines(IL-12,TNF-α,IL-1β,and IL-6).In addition,the vaccine had good in vivo biocompatibility,could protect the antigen from rapid degradation,and increased the retention time in lymph ***_(2)nanoparticles-in-CaCO_(3)microparticles were an excellent carrier for antigen and adjuvant ***,this study can provide some information on the design of microscale carriers for vaccine delivery systems.

关键词： Antigen delivery Cross-presentation Microparticle Silica nanoparticle Bone marrow dendritic cell

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

海藻酸盐水凝胶治疗心肌梗死的研究进展

引用

国际生物医学工程杂志 2024年第3期47卷 268-274页

作者：朱恩灿王伟伟张闯年中国医学科学院、北京协和医学院生物医学工程研究所天津300192

心肌梗死给人类健康带来严重威胁,可注射水凝胶可为梗死心肌区域提供机械支撑,还可负载药物、生物活性因子或细胞等,改善梗死微环境,诱导心肌组织再生,在治疗心肌梗死方面具有独特优势。海藻酸盐水凝胶以其类细胞外基质特性、易修饰和... 详细信息

心肌梗死给人类健康带来严重威胁,可注射水凝胶可为梗死心肌区域提供机械支撑,还可负载药物、生物活性因子或细胞等,改善梗死微环境,诱导心肌组织再生,在治疗心肌梗死方面具有独特优势。海藻酸盐水凝胶以其类细胞外基质特性、易修饰和生物组织相容性良好等特点被广泛研究。主要综述了近年来处于实验室研究阶段的复合水凝胶海藻酸盐水凝胶和生物活性海藻酸盐水凝胶以及临床研究阶段海藻酸盐水凝胶Algisyl-LVRTM、IK-5001、TCMR和Merike TM在心肌梗死治疗中的应用。

关键词：海藻酸盐水凝胶心肌梗死

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration

引用

Bioactive Materials 2023年第11期29卷 251-264页

作者： Qinghua Li Huijuan Song Shuangyang Li Pengbo Hu chuangnian zhang Ju zhang Zujian Feng Deling Kong Weiwei Wang Pingsheng Huang Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin300192China Institute of Radiation Medicine Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin300192China Department of Polymer Science and Engineering Key Laboratory of Systems BioengineeringSchool of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Emergency Department of Binzhou Medical University Hospital BinzhouShandong Province256600China State Key Laboratory of Medicinal Chemical Biology College of Life SciencesNankai UniversityTianjin300071China

Excessive reactive oxygen species(ROS)at severe burn injury sites may promote metabolic reprogramming of macrophages to induce a deteriorative and uncontrolled inflammation cycle,leading to delayed wound healing and ***,a novel bioactive,anti-fouling,flexible polyzwitterionic hydrogel encapsulated with epigallocatechin gallate(EGCG)-copper(Cu)capsules(termed as EGCG-Cu@CBgel)is engineered for burn wound management,which is dedicated to synergistically exerting ROS-scavenging,immune metabolic regulation and pro-angiogenic ***-Cu@CBgel can scavenge ROS to normalize intracellular redox homeostasis,effectively relieving oxidative damages and blocking proinflammatory signal ***,EGCG-Cu can inhibit the activity of hexokinase and phosphofructokinase,alleviate accumulation of pyruvate and convert it to acetyl coenzyme A(CoA),whereby inhibits glycolysis and normalizes tricarboxylic acid(TCA)***,metabolic reprogramming of macrophages by EGCG-Cu downregulates M1-type polarization and the expression of proinflammatory cytokines both in vitro and in ***,copper ions(Cu^(2+))released from the hydrogel facilitate ***-Cu@CBgel significantly accelerates the healing of severe burn wound via promoting wound closure,weakening tissue-damaging inflammatory responses and enhancing the remodeling of pathological ***,this study demonstrates the great potential of bioactive hydrogel dressing in treating burn wounds without unnecessary secondary damage to newly formed skin,and highlights the importance of immunometabolism modulation in tissue repair and regeneration.

关键词： ROS scavenging Hydrogel dressing EGCG-Cu capsule Metabolic reprogramming Burn wound healing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tumor acid microenvironment-activated self-targeting&splitting gold nanoassembly for tumor chemo-radiotherapy

引用

Bioactive Materials 2022年第1期7卷 377-388页

作者： Xiaomin Li Licheng Yu chuangnian zhang Xiaoyan Niu Mengjie Sun Zichao Yan Wei Wang Zhi Yuan Key Laboratory of Functional Polymer Materials of Ministry of Education College of ChemistryNankai UniversityTianjin300071China Tianjin Key Laboratory of Biomaterial Research Biomedical Barriers Research CenterInstitute of Biomedical EngineeringChinese Academy of Medical Science&Peking Union Medical CollegeTianjin300192China Collaborative Innovation Center of Chemical Science and Engineering Nankai UniversityTianjin300071China

Low accumulation and penetration of nanomedicines in tumor severely reduce therapeutic ***,a pH-responsive gold nanoassembly is designed to overcome these *** glycol linked raltitrexed(RTX,target ligand and chemotherapy drug)and two tertiary amine molecules(1-(2-aminoethyl)pyrrolidine and N,N-dibutylethylenediamine)are modified on the surface of the 6-nm gold nanoparticles by lipoic acid to form gold nanoassembly defined as Au-NNP(RTX).The Au-NNP(RTX)nanoassembly could remain at about 160 nm at the blood circulation(pH 7.4),while split into 6-nm gold nanoparticles due to tertiary amine protonation at tumor extracellular pH(pH 6.8).This pH-responsive disassembly behavior endows Au-NNP(RTX)better tumor tissue permeability through the better diffusion brought by the size ***,after disassembly,more RTXs on the surface of gold nanoparticles are exposed from the shielded state of assembly along with 2.25-fold augment of cellular uptake *** importantly,the results show that Au-NNP(RTX)possesses of high tumor accumulation and effective tumor penetration,thereby enhancing the tumor chemo-radiotherapy efficiency.

关键词： Gold nanoassembly Disassemble Penetration Self-target Chemo-radiotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dual pH-responsive “charge-reversal like” gold nanoparticles to enhance tumor retention for chemo-radiotherapy

引用

Nano Research 2019年第11期12卷 2815-2826页

作者： Xiaolei zhang chuangnian zhang Mingbo Cheng Yahui zhang Wei Wang Zhi Yuan Key Laboratory of Functional Polymer Materials of the Ministry of Education Institute of Polymer ChemistryCollege of ChemistryNankai UniversityTianjin300071China Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Science&Peking Union Medical CollegeTianjin300192China

The strategy of pH-responsive aggregation in tumor micro-environment(TME)provides an intriguing platform for enhancing tumor retention and exerting therapeutic effects *** this work,we have designed an intelligent dual pH-responsive self-aggregating nano gold system(Au@PAH-Pt/DMMA)for the combined chemo-radiotherapy,in which a“charge-reversal like”strategy was utilized to realize irreversible stable aggregation and pH-specific release of cisplatin prodrug in *** aggregation increases the cellular uptake of Au@PAH-Pt/DMMA by 55%–60%,and the cellular uptake of Pt after X-ray irradiation can be further enhanced by 80%.Additionally,responsive aggregation greatly slows down the rate of efflux from tumor in *** system not only promotes B16 cell apoptosis as a chemotherapeutic agent(30.4%),it also enhances the effect of chemo-radiotheray(CRT)by promoting apoptosis as a radiosensitizer(55.3%).The colony formation assay results were fitted to cell survival curve of B16 cells and the sensitization enhancement ratio(SER)was calculated to be 1.29,which shows a good radiosensitizing *** exposed to X-ray,this nanoplatform reached the ideal therapeutic effect,and the tumor inhibition rate of Au@PAH-Pt/DMMA reached 91.6%with low drug administration frequency and dose of ***,the dual pH-responsive nanoparticles Au@PAH-Pt/DMMA could effectively enhance tumor therapeutic efficiency by combined chemo-radiotherapy,which provides a potential method for clinical transformation of cancer treatment.

关键词： aggregation enhanced retention radiosentisizers pH-responsive charge-reversal

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Radial porous SiO_(2) nanoflowers potentiate the effect of antigen/adjuvant in antitumor immunotherapy

引用

Frontiers of Chemical Science and Engineering 2021年第5期15卷 1296-1311页

作者： chuangnian zhang Ying Dong Jing Gao Xiaoli Wang Yanjun Jiang Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin300192China School of Chemical Engineering and Technology Hebei University of TechnologyTianjin300130China

Here,we reported a cancer nanovaccine based on SiO_(2)nanoflowers with a special radial pore structure,which greatly enhanced cross-presentation and induced the production of cytotoxic T lymphocyte cells secreting granzymes B and interferon-γ.The antigen ovalbumin was covalently conjugated onto the as-synthesized hierarchical SiO_(2)nanoflowers,and the adjuvant cytosine-phosphate-guanine was electrostatically adsorbed into their radial pore by simple mixing before *** nanovaccine exhibited excellent storage stability without antigen release after 27 days of incubation,negligible cytotoxicity to dendritic cells,and a high antigen loading capacity of 430±66 mg·g^(−1)***,the nanovaccine could be internalized by dendritic cells via multiple *** the enhancement of antigen/adjuvant uptake and lysosome escape of antigen were ***,in vitro culture of bone marrow-derived dendritic cells in the presence of nanovaccine proved the activation of dendritic cells and antigen cross-presentation as well as secretion of proinflammatory ***,in vivo study verified the targeting of nanovaccine to draining lymph nodes,the complete suppression of tumor in six out of ten mice,and the triggering of notable tumor growth ***,the present results indicated that the nanovaccine can be served as a potential therapeutic vaccine to treat cancer.

关键词： silica nanoflower antigen delivery cancer immunotherapy nanovaccine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Glycyrrhetinic acid-modified nanoparticles for drug delivery: Preparation and characterization

引用

Chinese Science Bulletin 2009年第10期54卷 3121-3126页

作者： TIAN Qin WANG Wei HE XiaoTing ZHU XiaoCui HUANG Wei zhang chuangnian YUAN Zhi CHEN XueSi Key Laboratory of Functional Polymer Materials Ministry of Education Institute of Polymer Chemistry Nankai University Tianjin 300071 China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun 130022 China

In this work, glycyrrhetinic acid-modified chitosan (mGA-suc-CTS) used as liver targeted carrier for drug delivery, was prepared via hemisuccinate as a bridged group. The structure of the product was confirmed by IR and NMR methods and the degree of substitution (DS) of glycyrrhetinic acid groups was estimated via elemental analysis. Nanoparticles were formed by ionic gelation methold. The drug-loading and release behavior of the nanoparticles were investigated using BSA as the model drug. The results indicated that the carrier with a highest DS of 5.19% could be got and the DS was controlled by changing reaction temperature or feed ratio. BSA could be entrapped into the nanoparticles with the drug-loading ratio of 26.3% and the encapsulation efficiency of 81.5%. A sustained release over an 11-day period was observed in pH 7.4 in vitro.

关键词：药物输送纳米粒子甘草次酸酸改性表征制备核磁共振方法牛血清白蛋白

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Self-assembled PEG-b-PDPA-b-PGEM copolymer nanoparticles as protein antigen delivery vehicles to dendritic cells:preparation,characterization and cellular uptake

引用

Regenerative Biomaterials 2017年第1期4卷 11-20页

作者： Pan Li Junhui Zhou Pingsheng Huang chuangnian zhang Weiwei Wang Chen Lia Deling Kong Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300192China School of Chemical Engineering and Technology Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin UniversityTianjin 300072China

Antigen uptake by dendritic cells(DCs)is a key step for initiating antigen-specific T cell *** the present study,novel synthetic polymeric nanoparticles were prepared as antigen delivery vehicles to improve the antigen uptake by ***-defined cationic and acid-responsive copolymers,monomethoxy poly(ethylene glycol)-block-poly(2-(diisopropyl amino)ethyl methacrylate)-block-poly(2-(guanidyl)ethyl methacrylate)(mPEG-b-PDPA-b-PGEM,PEDG)were synthesized by reversible addition-fragmentation chain transfer polymerization of 2-(diisopropylamino)ethyl methacrylate and N-(tert-butoxycarbonyl)amino ethyl methacrylate monomers,followed by deprotection of tert-butyl protective groups and guanidinylation of obtained primary amines.1H NMR,13C NMR and GPC results indicated the successful synthesis of well-defined PEDG *** copolymers could self-assemble into nanoparticles in aqueous solution,which were of cationic surface charges and showed acid-triggered disassembly contributed by PGEM and PDPA moieties,***,PEDG nanoparticles could effectively condense with negatively charged model antigen ovalbumin(OVA)to form OVA/PEDG nanoparticle formulations with no influence on its secondary and tertiary structures demonstrating by far-UV circular dichroism and UV-vis *** vitro antigen cellular uptake by bone marrow DCs(BMDCs)indicated using PEDG nanoparticles as antigen delivery vehicles could significantly improve the antigen uptake efficiency of OVA compared with free OVA or the commercialized Alum ***,as the surface cationic charges of OVA/PEDG nanoparticle formulations reduced,the uptake efficiency decreased ***,our work suggests that guanidinylated,cationic and acid-responsive PEDG nanoparticles represent a new kind of promising antigen delivery vehicle to DCs and hold great potential to serve as immunoadjuvants in the development of vaccines.

关键词： guanidyl synthetic copolymer nanoparticles antigen delivery dendritic cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cytotoxicity of liver targeted drug-loaded alginate nanoparticles

引用

Science China Chemistry 2009年第9期52卷 1382-1387页

作者： zhang chuangnian WANG Wei WANG ChunHong TIAN Qin HUANG Wei YUAN Zhi CHEN XueSi Key Laboratory of Functional Polymer Materials Ministry of EducationInstitute of Polymer ChemistryNankai UniversityTianjin 300071China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

In this study, novel liver targeted doxorubicin (DOX) loaded alginate (ALG) nanoparticles were prepared by CaCl2 crosslinking method. Glycyrrhetinic acid (GA, a liver targeted molecule) modified alginate (GA-ALG) was synthesized in a heterogeneous system, and the structure of GA-ALG and the substitu-tion degree of GA were analyzed by 1H NMR, FT-IR and elemental analysis. The drug release profile under the simulated physiological condition and cytotoxicity experiments of drug-loaded GA-ALG nanoparticles were carried out in vitro. Transmission electron micrographs (TEM) and dynamic light scattering (DLS) analysis showed that drug-loaded GA-ALG nanoparticles have spherical shape structure with the mean hydrodynamic diameter around 214 ± 11 nm. The drug release was shown to last 20 days, and the MTT assay suggested that drug-loaded GA-ALG nanoparticles had a distinct kill-ing effect on 7703 hepatocellular carcinoma cells.

关键词： liver targeted glycyrrhetinic acid alginate cytotoxicity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Supramolecular co-assembly of self-adjuvanting nanofibrious peptide hydrogel enhances cancer vaccination by activating MyD88-dependent NF-κB signaling pathway without inflammation

引用

Bioactive Materials 2021年第11期6卷 3924-3934页

作者： Qi Su Huijuan Song Pingsheng Huang chuangnian zhang Jing Yang Deling Kong Weiwei Wang Department of Polymer Science and Engineering School of Chemical Engineering and TechnologyCollaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin UniversityTianjin300072China Institute of Radiation Medicine Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin300192China Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin300192China State Key Laboratory of Medicinal Chemical Biology College of Life SciencesNankai UniversityTianjin300071China

Peptide vaccine targeting tumor-specific antigens is a promising cancer treatment ***,peptide vaccines are commonly low-immunogenic,leading to suboptimal antitumor T-cell *** peptide vaccination approaches are challenged by the variability of peptide physicochemical characters and vaccine formulations,flexibility,and the broad ***,the supramolecular co-assembly of antigen epitope-conjugated peptides(ECPs)targeting CD8 or CD4 T-cell receptors was used to engineer a nanofibrious hydrogel vaccine *** approach provided precise and tunable loading of peptide antigens in nanofibers,which notably increased the antigen uptake,cross-presentation,and activation of dendritic cells(DCs).Immunization in mice indicated that the co-assembled peptide hydrogel did not induce local inflammation responses and elicited significantly promoted T-cell immunity by activating the MyD88-dependent NF-κB signaling pathway in *** of mice using co-assembled peptide vaccine stimulated both enhanced CD8 and CD4 T cells against EG.7-OVA tumors without additional immunoadjuvants or delivery systems,and resulted in a more remarkable cancer immunotherapy efficacy,compared with free peptide vaccine or aluminum-adjuvanted peptide ***,peptide co-assembly demonstrated by three independent pairs of ECPs is a facile,customizable,and chemically defined approach for co-delivering peptide antigens in self-adjuvanting hydrogel vaccines that could induce stronger anticancer T-cell responses.

关键词： Cancer immunotherapy Vaccination Peptide Co-assembly Self-adjuvanting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：