Programmed nanoparticle-loaded microparticles for effective antigen/adjuvant delivery
作者机构：Tianjin Key Laboratory of Biomaterial ResearchInstitute of Biomedical EngineeringChinese Academy of Medical Sciences&1 Peking Union Medical CollegeTianjin 300192China School of Chemical Engineering and Technology.Hebei University of Technology.Tianjin.300130China NHC Key Laboratory of Hormones and DevelopmentTianjin Key Laboratory of Metabolic DiseasesChu Hsien-I Memorial Hospital&Tianjin Institute of EndocrinologyTianjin Medical UniversityTianjin 300134China
出 版 物：《Particuology》 (颗粒学报（英文版）)
年 卷 期：2022年第20卷第1期
页 面：77-89页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 07[理学] 0817[工学-化学工程与技术] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 100401[医学-流行病与卫生统计学] 0702[理学-物理学] 10[医学]
基 金：The authors thank the National Natural Science Foundation of China(grant No.81972899) Natural Science Foundation of Tianjin City(grant No.18JCQNJC14500) CAMS Innovation Fund for Medical Sciences(grant No.2017-I2M-3-022) Specific Program for High-Tech Leader&Team of Tianjin Government,Tianjin Innovation and Promotion Plan Key Innovation Team of Immunoreactive Biomaterials
主 题：Antigen delivery Cross-presentation Microparticle Silica nanoparticle Bone marrow dendritic cell
摘 要：A microscale vaccine containing SiO_(2)nanoparticles loaded in CaC〇3 microparticles was constructed using the co-precipitation *** antigen ovalbumin(OVA)was covalently conjugated with SiO_(2)nanoparticles,and these nanoparticles and CpG were co-encapsulated into CaCO_(3)microparticles,generating a vaccine with a size of approximately 5.2μ*** electron microscopy(SEM),energy-dispersive X-ray(EDX),elemental mapping,and Fourier transform infrared(FTIR)analyses confirmed the successful preparation of the microscale vaccine;the vaccine had good storage stability without sustained antigen release,and negligible cytotoxicity to dendritic cells(DCs)and *** to SiO_(2)nanoparticles,the microscale vaccine can significantly improve antigen/adjuvant *** internalized the entire microscale vaccine into lysosomes via macropinocytosis,and an increase in antigen endo/lysosomal escape was observed by confocal User scanning microscopy(CLSM).Specifically,DCs pulsed with the vaccine were fully mature,expressing high levels of costimulatory molecules(CD40,CD80,and CD86),MHCⅡ,and MHCⅠand secreting high levels of proinflammatory cytokines(IL-12,TNF-α,IL-1β,and IL-6).In addition,the vaccine had good in vivo biocompatibility,could protect the antigen from rapid degradation,and increased the retention time in lymph ***_(2)nanoparticles-in-CaCO_(3)microparticles were an excellent carrier for antigen and adjuvant ***,this study can provide some information on the design of microscale carriers for vaccine delivery systems.
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