检索结果-南通市图书馆

Chinese Science Bulletin 2008年第19期53卷 2973-2980页

作者： yi chuandeng TANG ShuZhu ZHOU Yong LIANG GuoHua GONG ZhiYun GU MingHong Jiangsu Province Key Laboraty of Crop Genetics and Physiology/Key Laboraty of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China

Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chro- mosome pairing was identified and most of the chromosomes were univalent, which resulted in com- plete male sterility in the hybrid.

关键词：漂白亚麻纤维卷胚芽种间杂交技术

Isolation and Identification of a Functional Centromere Element in the Wild Rice Species Oryza granulata with the GG Genome

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2015年第12期42卷 699-702页

作者： chuandeng yi Mingsen Wang Wei Jiang Derong Wang Yong Zhou Zhiyun Gong Guohua Liang Minghong Gu Jiangsu Key Laboratory of Crop Genetics and Physiology Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University

The centromere of eukaryotic chromosomes is the crucial locus responsible for sister chromatid cohesion and for correct segregation of chromosomes to daughter cells during cell division. In the structural genomics era, centromeres represent the last frontiers of higher eukaryotic genomes because of their densely methylated, highly repetitive and, heterochromatic DNA （Hall et al., 2004）. Although these functions are conserved among all eukaryotes, centromeric DNA sequences are evolving rapidly （Jiang et al., 2003）.

关键词： FISH BAC Isolation and Identification of a Functional Centromere Element in the Wild Rice Species Oryza granulata with the GG Genome

Rice Interploidy Crosses Disrupt Epigenetic Regulation, Gene Expression, and Seed Development

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2018年第2期11卷 300-314页

作者： Limei Wang Jingya Yuan Yujie Ma Wu Jiao Wenxue Ye Dong-Lei Yang chuandeng yi Z. Jeffrey Chen State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University 1 Weigang Road Nanjing 210095 China Department of Molecular Biosciences Institute for Cellular and Molecular Biology Center for Computational Biology and Bioinformatics The University of Texasat Austin Austin TX 78712 USA Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China

Seed development in angiosperms requires a 2：1 maternal-to-paternal genome ratio （2m：lp） in the endo- sperm. When the ratio is disrupted, the seed development is impaired. Rice interploidy crosses result in endosperm failures, but the underlying molecular mechanisms remain unclear. Here, we report that the defective endosperm in rice interploidy crosses was associated with nonadditive expression of small RNAs and protein-coding genes. Interestingly, 24-nt small interfering RNAs were enriched in the 5＇ and 3＇ flanking sequences of nonadditively expressed genes in the interploidy crosses and were negatively associated with the expression of imprinted genes. Furthermore, some PRC2 family genes and DNA methylaUon-related genes including OsMETlb and OsCMT3a were upregulated in the 2x4 cross （polli- nating a diploid ＂mother＂ with a tetraploid ＂father＂） but repressed in the reciprocal cross. These different epigenetic effects could lead to precocious or delayed cellularization during endosperm development. Notably, many endosperm-preferred genes, including starch metabolic and storage protein genes during grain filling, were found to be associated with DNA methylation or H3K27me3, which are repressed in both 2×4 and 4 ×2 crosses. WUSCHEL homeobox2 （WOX2）-Iike （WOX2L）, an endosperm-preferred gene, was expressed specifically in the rice endosperm, in contrast to WOX2 expression in the Arabidopsis embryo. Disruption of WOX2L in transgenic rice by CRISPR/Cas9-mediated gene editing blocked starch and protein accumulation, resulting in seed abortion. In addition to gene repression, disrupting epigenetic process in the interploidy crosses also induced expression of stress-responsive genes. Thus, maintaining the 2m：lp genome ratio in the endosperm is essential for normal grain development in rice and other cereal crops.

关键词： seed polyploidy small RNA gene expression endosperm epigenetics

Development and characterization of synthetic amphiploids of Oryza sativa and Oryza latifolia

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2015年第23期60卷 2059-2062页

作者： chuandeng yi Mingsen Wang Wei Jiang Derong Wang Xiaojun Cheng ying Wang Yong Zhou Guohua Liang Minghong Gu Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University

Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subse- quently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molec- ular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chro- mosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72. Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.

关键词： Oryza sativa · Oryza latifolia· Colchicine · Amphiploid · Genomic in situhybridization

Wx^lv、the Ancestral Allele of Rice Waxy Gene

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2019年第8期12卷 1157-1166页

作者： Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu chuandeng yi Shuzhu Tang Minghong Gu Qiaoquan Liu Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding College of AgricultureYangzhou UniversityYangzhou 225009China Co-lnnovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou UniversityYangzhou 225009China

In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking *** effects of several Wx alleles on amylose content and the taste of cooked rice have been ***,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain *** we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose *** demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional *** data indicate that the Wx^lv allele played an important role in artificial selection and *** findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice.

关键词： Oryza sativa Waxy eating and cooking quality allelic variation artificial selection

Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification and diversity of functional centromere satell...

江苏省遗传学会2013年学术研讨会

作者： chuandeng yi Xibin Dai Xing Li Yong Zhou Guohua Liang Minghong Gu Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Key Laboratory of Plant Functional Genomics of Ministry of Education College of Agriculture Yangzhou University

The centromere is a key chromosomal component for sister chromatid cohesion and is the site for kinetochore assembly and spindle fiber attachment, allowing each sister chromatid to faithfully segregate to each daughter cell during cell division. It is not clear what types of sequences act as functional centromeres and how centromere sequences are organized in Oryza brachyantha, an FF genome species. In this study, we found that the three classes of centromere-specific CentO-F satellites（CentO-F1, CentO-F2 and CentO-F3） in O. brachyantha share no homology with the CentO satellites in O. sativa. The three classes of CentO-F satellites are all located within the chromosomal regions to which the spindle fibers attach and are characterized by megabase tandem arrays that are flanked by centromere-specific retrotransposons, CRR-F, in the O. brachyantha centromeres. Although these CentO-F satellites are quantitatively variable among 12 O. brachyantha centromeres, immunostaining with an antibody specific to CENH3 indicates that they are colocated with CENH3 in functional centromere regions. Our results demonstrate that the three classes of CentO-F satellites may be the major components of functional centromeres in O. brachyantha.

关键词： centromere Oryza brachyantha satellite DNA repetitive DNA

Isolation and Identification of functional centromere element in the wild rice species Oryza granulata with GG genome

在线全文

cnki会议

学校读者我要写书评

暂无评论

Isolation and Identification of functional centromere elemen...

江苏省遗传学会第九次会员代表大会暨学术研讨会

作者： chuandeng yi Mingsen Wang Wei Jiang Derong Wang Yong Zhou Zhiyun Gong Guohua Liang Minghong Gu Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University

The centromere is the important DNA region of eukaryotic chromosome for sister chromatid cohesion, kinetochore assembly and spindle fiber attachment,ensuring the faithful segregation of chromatids in mitosis and of homologous chromosomes during meiosis. In most eukaryotic chromosomes, various centromeric tandem repeats（satellite repeats） and centromere-specific retrotransposons were isolated. In genus Oryza, these related DNA elements have been identified from AA（Oryza sativa）, BB（***）, CC（*** and ***） and FF（***） genome, respectively. But little is known about GG（O. granulata） genome, which occupies the most basal position of the genus. In the present study, no typical centromere-specific satellite were found from four centromere candidate bacterial artificial chromosome（BAC） clones of O. granulata. But we found that the centromere retrotransposons of O. granulata（CRR-G）, which share high homology with the Gran3 LTR retrotransposons, are specific to GG genome by FISH assay. Immunostaining with an antibody specific to CENH3 reveals that the CRR-G elements are colocated with CENH3 in functional centromere regions to which the spindle fibers attach. Our results demonstrate that the CRR-G elements may be the major componets of functional centromere in O. granulata.

关键词： Oryza granulata centromere repetitive DNA retrotransposon

GNS4, a novel allele of DWARF11, regulates grain number and grain size in a high-yield rice variety

在线全文

cnki会议

学校读者我要写书评

暂无评论

GNS4, a novel allele of DWARF11, regulates grain number and ...

江苏省遗传学会2017年学术研讨会——“技术创新与遗传学发展”

作者： Yajun Tao Yong Zhou Jinyan Zhu Jun Miao Jun Liu Yanhua Liu chuandeng yi Zefeng Yang Zhiyun Gong Guohua Liang

Rice plays an extremely important role in food safety because it feeds more than half of the world’s population. Rice grain yield depends on biomass and the harvest index. An important strategy to break through the rice grain yield ceiling is to increase the biological yield. Therefore, genes associated with organ size are important targets for rice breeding. We characterized a rice mutant gns4(grain number and size on chromosome 4) with reduced organ size, fewer grains per panicle, and smaller grains compared with those of WT. Mapbased cloning indicated that the GNS4 gene, encoding a cytochrome P450 protein, is a novel allele of DWARF11(D11). A single nucleotide polymorphism(deletion) in the promoter region of GNS4 reduced its expression level in the mutant, leading to reduced grain number and smaller grains. Morphological and cellular analyses suggested that GNS4 positively regulates grain size by promoting cell elongation. Overexpression of GNS4 significantly increased organ size, 1000-grain weight, and panicle size, and subsequently enhanced grain yields in both the Nipponbare and Wuyunjing7(a high-yielding cultivar) backgrounds. These results suggest that GNS4 is key target site with possible applications in rice yield breeding. GNS4 was identified as a positive regulator of grain number and grain size in rice. Increasing the expression level of this gene in a high-yielding rice variety enhanced grain yield. GNS4 can be targeted in breeding programs to increase yields.

关键词： Rice GNS4/D11 Grain number Grain size Cell elongation

Identification and fine mapping of qPH6,a novel major QTL for plant height in rice

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification and fine mapping of qPH6,a novel major QTL fo...

江苏省遗传学会2014年学术研讨会

作者： Yuan Yuan Jun Miao Yajun Tao Peina Du Jun Wang Zhongde Wang Zhiyun Gong chuandeng yi Jinyan Zhu Guichun Dong Minghong Gu Yong Zhou Guohua Liang Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics Yangzhou University Institute of Food Crops Jiangsu Academy of Agricultural Sciences

Plant height is an important component that influences rice *** useful novel semi-dwarf gene is necessary for high-yield rice *** this study,C33084,a backcross introgression line derived from two parents contrasting in plant height(Balilla and Dular) was employed to locate a gene with a large effect on plant height by bulk segregant *** analysis of F2 and F4 generations,derived from a cross between C33084 and Balilla,revealed that the trait was controlled by the recessive gene qPH6,located on the short arm of chromosome *** mapping was carried out with a total of 1,791 recessive individuals from F2 and F4 generations,and the gene was localized to a 41-kb region containing three annotated genes according to available sequence annotation *** information could be helpful for future identification and isolation of the candidate *** also developed a pair of nearly isogenic lines,NIL(qph6) and NIL(qPH6),from the progenies derived from the cross between C33084 and *** order to determine the type of dwarf,with which qPH6 might have been associated,we measured the length of the panicles as well as the internodes of NIL(qph6) and NIL(qPH6).The major difference of the plant height between NIL(qPH6) and NIL(qph6) was caused by the length of the basal *** order to determine the factors responsible for the differences in the basal internodes,transverse sections,longitudinal sections,and stem epidermal layers were employed to histologically analyze the internode structure of NIL(qph6) and NIL(qPH6).The results indicated that the increased height of the stems in NILs was mostly due to an increased cell proliferation rather than cell size in the basal *** evaluation of the nearly isogenic lines NIL(qPH6) and NIL(qph6) revealed that qPH6 had no pleiotropic on other *** discovery of qPH6 might be useful for the design and breeding of crops with a high grain yield and quality.

关键词：

Mutation of the Light-induced Yellow Leaf 1 Gene, Which Encodes a Geranylgeranyl Reductase, Affects Chlorophyll Biosynthesis and Light Sensitivity in Rice

在线全文

cnki会议

学校读者我要写书评

暂无评论

Mutation of the Light-induced Yellow Leaf 1 Gene, Which Enco...

江苏省遗传学会2013年学术研讨会

作者： Yuan Yuan Yong Zhou Zhiyun Gong Zefeng Yang Jinyan Zhu Man Wang Fuhai Yuan Shujun Wu Zhiqin Wang chuandeng yi Tinghua Xu MyongChol Ryom Minghong Gu Guohua Liang

Chlorophylls（Chls） are crucial for capturing light energy for photosynthesis. Although several genes responsible for Chl biosynthesis were characterized in rice（Oryza sativa）, the genetic properties of the hydrogenating enzyme involved in the final step of Chl synthesis remain unknown. In this study, we characterized a rice light-induced yellow leaf 1-1（lyl1-1） mutant that is hypersensitive to high-light and defective in the Chl synthesis. Light-shading experiment suggested that the yellowing of lyl1-1 is light-induced. Map-based cloning of LYL1 revealed that it encodes a geranylgeranyl reductase. The mutation of LYL1 led to the majority of Chl molecules are conjugated with an unsaturated geranylgeraniol side chain. LYL1 is the firstly defined gene involved in the reduction step from Chl-geranylgeranylated（ChlGG） and geranylgeranyl pyrophosphate（GGPP） to Chl-phytol（ChlPhy） and phytyl pyrophosphate（PPP） in rice. LYL1 can be induced by light and suppressed by darkness which is consistent with its potential biological functions. Additionally, the lyl1-1 mutant suffered from severe photooxidative damage and displayed a drastic reduction in the levels of α-tocopherol and photosynthetic proteins. We concluded that LYL1 also plays an important role in response to high-light in rice.

关键词： rice Chl biosynthesis light-induced yellow leaf geranylgeranyl reductase α-tocopherol