Isolation and Identification of functional centromere element in the wild rice species Oryza granulata with GG genome
作者单位：Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University
会议名称：《江苏省遗传学会第九次会员代表大会暨学术研讨会》
会议日期：2015年
关 键 词：Oryza granulata centromere repetitive DNA retrotransposon
摘 要：The centromere is the important DNA region of eukaryotic chromosome for sister chromatid cohesion, kinetochore assembly and spindle fiber attachment,ensuring the faithful segregation of chromatids in mitosis and of homologous chromosomes during meiosis. In most eukaryotic chromosomes, various centromeric tandem repeats（satellite repeats） and centromere-specific retrotransposons were isolated. In genus Oryza, these related DNA elements have been identified from AA（Oryza sativa）, BB（***）, CC（*** and ***） and FF（***） genome, respectively. But little is known about GG（O. granulata） genome, which occupies the most basal position of the genus. In the present study, no typical centromere-specific satellite were found from four centromere candidate bacterial artificial chromosome（BAC） clones of O. granulata. But we found that the centromere retrotransposons of O. granulata（CRR-G）, which share high homology with the Gran3 LTR retrotransposons, are specific to GG genome by FISH assay. Immunostaining with an antibody specific to CENH3 reveals that the CRR-G elements are colocated with CENH3 in functional centromere regions to which the spindle fibers attach. Our results demonstrate that the CRR-G elements may be the major componets of functional centromere in O. granulata.