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Primary liver transplantation vs liver resection followed by transplantation for transplantable hepatocellular carcinoma:Liver functional quality and tumor characteristics matter

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World Journal of Gastrointestinal Surgery 2013年第1期5卷 5-8页

作者： Mehmet Fatih Can christopher b Hughes Division of Gastrointestinal Surgery Department of SurgeryGulhane School of MedicineEtlik 06018Ankara AnkaraTurkey Thomas Starzl Transplantation Institute University of Pittsburgh Medical CenterPittsburghPA 15213United States

Liver resection(LR) and primary liver transplantation(LT) are two potentially curative treatment modalities for patients with hepatocellular carcinoma(HCC).If an underlying chronic liver disease exists,however,making a decision on which method should be selected is *** a patient has no chronic liver disease,LR may be the preferable option with salvage transplantation(ST) in mind in case of *** of a moderate-to-severe liver failure accompanying HCC usually warrants primary *** treatment of patients with HCC and early-stage chronic liver disease remains *** advantages of "LR-followed-by-STif-needed" strategy include less complicated index operation,no need for immunosuppression,use of donor livers for other patients in today's organ shortage setting and comparable survival ***,primary LT has its own advantages as it also treats underlying chronic liver disease with carcinogenic potential,removes undetected tumor nodules and potentially eliminates need for a *** article recently published by Fuks et al in Hepatology offers an approach by which selecting between LR-followed-by-ST and immediate LT might be *** we discuss the results of the aforementioned report in the light of currently available knowledge.

关键词： Hepatocellular carcinoma Chronic liver disease Liver transplantation Liver resection Salvage transplantation Survival

Probing the Impact of Solvent on the Strength of Lewis Acids via Fluorescent Lewis Adducts

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Precision Chemistry 2023年第1期1卷 49-56页

作者： Amy E.Laturski Joshua R.Gaffen Paul Demay-Drouhard christopher b.Caputo Thomas baumgartner Department of Chemistry York UniversityTorontoON M3J 1P3Canada

Various methods have been developed to measure the strength of a Lewis acid.A major challenge for these measurements lies in the complexity that arises from variable solvent interactions and perturbations of Lewis acids as their reaction environment ***,we investigate the impact of solvent effects on Lewis acids for the first time as measured by the fluorescent Lewis adduct(FLA)*** binding of a Lewis acid in various solvents reveals a measurable dichotomy between both polarity and donor ability of the *** not strictly separable,we observe that the influence of solvent polarity on Lewis acid unit(LAU)values is distinctly opposite to the influence of donor *** dichotomy was confirmed by titration data,illustrating that solvation effects can be appropriately and precisely gauged by the FLA method.

关键词： Fluorescence Fluorescent Lewis adducts Lewis acids Effective Lewis acidity Solvent polarity Solvent donor strength

Electrochemically Grown Ultrathin Platinum Nanosheet Electrodes with Ultralow Loadings for Energy-Saving and Industrial-Level Hydrogen Evolution

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Nano-Micro Letters 2023年第9期15卷 257-274页

作者： Lei Ding Zhiqiang Xie Shule Yu Weitian Wang Alexander Y.Terekhov brian K.Canfield christopher b.Capuano Alex Keane Kathy Ayers David A.Cullen Feng-Yuan Zhang Nanodynamics and High-Efficiency Lab for Propulsion and Power Department of MechanicalAerospace&Biomedical EngineeringUT Space Institute(University of Tennessee-Knoxville)TullahomaTN 37388USA Center for Laser Applications UT Space Institute(University of Tennessee-Knoxville)TullahomaTN 37388USA Nel Hydrogen WallingfordCT 06492USA Oak Ridge National Laboratory Center for Nanophase Materials SciencesOak RidgeTN 37831USA

Nanostructured catalyst-integrated electrodes with remarkably reduced catalyst loadings,high catalyst utilization and facile fabrication are urgently needed to enable cost-effective,green hydrogen production via proton exchange membrane electrolyzer cells(PEMECs).Herein,benefitting from a thin seeding layer,bottom-up grown ultrathin Pt nanosheets(Pt-NSs)were first deposited on thin Ti substrates for PEMECs via a fast,template-and surfactant-free electrochemical growth process at room temperature,showing highly uniform Pt surface coverage with ultralow loadings and vertically well-aligned nanosheet *** with an anode-only Nafion 117 catalyst-coated membrane(CCM),the Pt-NS electrode with an ultralow loading of 0.015 mgPt cm−2 demonstrates superior cell performance to the commercial CCM(3.0 mgPt cm^(−2)),achieving 99.5%catalyst savings and more than 237-fold higher catalyst *** remarkable performance with high catalyst utilization is mainly due to the vertically well-aligned ultrathin nanosheets with good surface coverage exposing abundant active sites for the electrochemical ***,this study not only paves a new way for optimizing the catalyst uniformity and surface coverage with ultralow loadings but also provides new insights into nanostructured electrode design and facile fabrication for highly efficient and low-cost PEMECs and other energy storage/conversion devices.

关键词： Seeding layer Electrochemically grown Pt nanosheet Ultralow loadings High catalyst utilization Hydrogen evolution

Molecular anatomy and pathogenic actions of Helicobacter pylori CagA that underpin gastric carcinogenesis

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Cellular & Molecular Immunology 2020年第1期17卷 50-63页

作者： Atsushi Takahashi-Kanemitsu christopher T.Knight Masanori Hatakeyama Department of Microbiology Graduate School of MedicineThe University of TokyoTokyoJapan

Chronic infection with Helicobacter pylori cagA-positive strains is the strongest risk factor for gastric *** cagA gene product,CagA,is delivered into gastric epithelial cells via the bacterial type IV secretion *** CagA then undergoes tyrosine phosphorylation at the Glu-Pro-Ile-Tyr-Ala(EPIYA)motifs in its C-terminal region and acts as an oncogenic scaffold protein that physically interacts with multiple host signaling proteins in both tyrosine phosphorylation-dependent and-independent *** of CagA using in vitro cultured gastric epithelial cells has indicated that the nonphysiological scaffolding actions of CagA cell-autonomously promote the malignant transformation of the cells by endowing the cells with multiple phenotypic cancer hallmarks:sustained proliferation,evasion of growth suppressors,invasiveness,resistance to cell death,and genomic *** expression of CagA in mice leads to in vivo oncogenic action of CagA without any overt *** in vivo oncogenic activity of CagA is further potentiated in the presence of chronic *** Helicobacter pylori infection triggers a proinflammatory response in host cells,a feedforward stimulation loop that augments the oncogenic actions of CagA and inflammation is created in CagA-injected gastric *** that Helicobacter pylori is no longer colonized in established gastric cancer lesions,the multistep nature of gastric cancer development should include a“hit-and-run”process of CagA ***,acquisition of genetic and epigenetic alterations that compensate for CagA-directed cancer hallmarks may be required for completion of the“hit-and-run”process of gastric carcinogenesis.

关键词： cagA/CagA Helicobacter pylori SHP2 PAR1 inflammation “hit-and-run”carcinogenesis

Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells

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中国病理生理杂志 2012年第11期 2049-2050页

作者： Sarathi MANI christopher LAI Department of biology Lakehead UniversityThunder BayON P7B 5E1Canada Northern Ontario School of Medicine Lakehead UniversityThunder BayON P7B 5E1Canada

Hydrogen sulfide(H2S)can be endogenously generated from cystathionine gamma-lyase(CSE)in cardiovascular system,offering cardiovascular *** is also known that the lower risk of cardiovascular diseases in female is part... 详细信息

关键词： cell CSE

MicroRNA-let-7a promotes E2F-mediated cell proliferation and NFKb activation in vitro

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Cellular & Molecular Immunology 2014年第1期11卷 79-93页

作者： Cristen b Chafin Nicole L Regna David L Caudell christopher M Reilly Department of biomedical Sciences & Pathobiology Virginia-Maryland Regional College of Veterinary Medicine Virginia Polytechnic Institute and StateUniversity Blacksburg VA USA Department of Pathology/Comparative Medicine Wake Forest University Primate Center Wake Forest School ofMedicine Winston-Salem NC USA Edward Via College of Osteopathic Medicine Blacksburg VA USA

Epigenetic factors, including altered microRNA （miRNA） expression, may contribute to aberrant immune cell function in systemic lupus erythematosus （SLE）. MiRNA-let-7a （let-7a） has been shown to directly alter cell cycle progression and proinflammatory cytokine production. Due to the crucial role of let-7a in cell division and inflammation, we investigated let-7a-mediated proliferation and NFKb translocation in J774A. 1 macrophages and MES 13 mesangial cells in vitro. In immune-stimulated cells transfected with let-7a, cell proliferation was significantly increased over time. There was a significant increase in the number of immune-stimulated cells in S and G2 phases. Immune-stimulated cells overexpressing let-7a had increased nuclear translocation of NFKb. bioinformatical analysis revealed that the E2F family, critical regulators of the G1-S transition, has potential binding sites for let-7a in their mRNA transcripts. Let-7a overexpression significantly increased the expression of the cell cycle activator E2F2 and increased retinoblastoma protein （Rb） phosphorylation in immune-stimulated cells. The cell cycle inhibitor E2F5 was significantly decreased in let-7a-transfected cells that were immune-stimulated. bioinformatical analysis revealed E2F2 and NFKb are transcription factors predicted to regulate the let-7a promoter. We analyzed transcriptional regulation of let-7a by real-time RT-PCR using chromatin immunoprecipitation with E2F2 and NFKb antibodies. There was an increase in E2F2 and NFKb binding in DNA enriched for the let-7a promoter in immune-stimulated cells. Silencing E2F2 or NFKb significantly decreased let-7a expression and IL-6 production in immune-stimulated cells. Taken together, our results suggest that overexpression of let-7a may contribute to hyperplasia and the proinflammatory response in SLE.

关键词： cell proliferation E2F microRNA-let-7a NF~:b systemic lupus erythematosus

Lenormandins A—G,new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii ***.,recognised by chemotaxonomic data

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Fungal Diversity 2015年第2期71卷 165-184页

作者： Eric Kuhnert Frank Surup Esteban b.Sir christopher Lambert Kevin D.Hyde Adriana I.Hladki Andrea I.Romero Marc Stadler Department of Microbial Drugs Helmholtz-Zentrum für Infektionsforschung GmbHInhoffenstrasse 738124 BraunschweigGermany German Centre for Infection Research(DZIF) partner site Hannover-Braunschweig38124 BraunschweigGermany Fundación Miguel Lillo Institute of MycologyMiguel Lillo 251San Miguel de Tucumán 4000TucumánArgentina Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Av.Rivadavia 1917Buenos Aires C1033AAJArgentina Institute of Excellence in Fungal Research and School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Department of biodiversity and Experimental biology University of Buenos AiresInt.Güiraldes 2620Buenos Aires C1428EHAArgentina

Seven new azaphilone pigments named lenormandins A–G were isolated from stromata(fruiting bodies)of the xylariaceous fungus,Hypoxylon lenormandii using preparative High Performance Liquid Chromatography(HPLC)and their structures were elucidated by NMR spectroscopy,high resolution mass spectrometry and other spectral *** occurrence in herbarium specimens(including various type materials collected in the 19th and early 20th century)and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection(HPLC-DAD/MS),revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa,*** and an undescribed fungus from Sri Lanka,for which we propose the name *** status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences,and by detailed morphological studies.

关键词： Chemotaxonomy Hypoxylon Phylogeny Secondary metabolites Systematics Xylariaceae

High tolerance to mutations in a Chlamydia trachomatis peptide deformylase loop

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World Journal of biological Chemistry 2011年第5期2卷 90-97页

作者： christopher b Oey Christal Lewis John E Kerrigan Department of Physiology and biophysics Robert Wood Johnson Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayNJ 08854United States Cancer Institute of New Jersey Robert Wood Johnson Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayNew BrunswickNJ 08903 United States

AIM:To determine if and how a loop region in the peptide deformylase(PDF)of Chlamydia trachomatis regulates enzyme function. METHODS:Molecular dynamics simulation was used to study a structural model of the chlamydial PDF(cPDF) and predict the temperature factor per residue for the protein backbone ***-directed mutagenesis was performed to construct cPDF *** properties of the resulting variants were determined by an enzyme assay using formyl-Met-Ala-Ser as a substrate. RESULTS:In silico analysis predicted a significant increase in atomic motion in the DGELV sequence(residues 68-72)of a loop region in a cPDF mutant,which isresistant to PDF inhibitors due to two amino acid substitutions near the active site,as compared to wild-type *** D68R and D68R/E70R cPDF variants demonstrated significantly increased catalytic *** E70R mutant showed only slightly decreased efficiency. Although deletion of residues 68-72 resulted in a nearly threefold loss in substrate binding,this deficiency was compensated for by increased catalytic efficiency. CONCLUSION:Movement of the DGELV loop region is involved in a rate-limiting conformational change of the enzyme during ***,there is no stringent sequence requirement for this region for cPDF enzyme activity.

关键词： Antibacterial Chlamydia Peptide deformylase Sexually transmitted infection

Phylogenetic and chemotaxonomic resolution of the genus Annulohypoxylon(Xylariaceae)including four new species

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Fungal Diversity 2017年第4期85卷 1-43页

作者： Eric Kuhnert Esteban b.Sir christopher Lambert Kevin D.Hyde Adriana I.Hladki Andrea I.Romero Manfred Rohde Marc Stadler Department of Microbial Drugs Helmholtz-Zentrum fur Infektionsforschung GmbHInhoffenstrasse 738124 BrunswickGermany German Centre for Infection Research(DZIF) partner site Hannover-Braunschweig38124 BrunswickGermany Fundacion Miguel Lillo CONICETInstitute of MycologyMiguel Lillo 251San Miguel de Tucuman 4000TucumanArgentina Fundacion Miguel Lillo Institute of MycologyMiguel Lillo 251San Miguel de Tucuman 4000TucumanArgentina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Instituto de Micologia y botanica(INMIbO) UBACONICETDepto.Biodiversidad y Biologia ExperimentalFCENAv.Int.Guiraldes 2620C1428EHA Ciudad Autonoma de Buenos AiresArgentina Central Facility for Microscopy Helmholtz-Zentrum fur Infektionsforschung GmbHInhoffenstrasse 738124 BrunswickGermany

This study deals with an extensive evaluation focusing on phylogenetic and chemotaxonomic infrageneric relationships of the genus Annulohypoxylon(Xylariaceae,Ascomycota),whose species are ubiquitously associated with seed plants as endophytes and saprotrophs in all forested areas of the *** evidence from phylogenetic,chemotaxonomic and morphological data,various varieties within the genus are raised to species level,leading to the new combinations for ***(:*** ***),***(:*** ***),and ***(:*** ***).Annulohypoxylon substygium *** applied for *** *** the four new tropical and subtropical species ***,***,*** and *** are ***,*** is excluded from the genus as its morphological characters disagree with the generic concept,the recently erected *** is synonymized with *** and *** has been reassigned to the genus *** addition,the key taxa *** and *** have been reinvestigated and epitypes have been *** the first time,a dichotomous key to the genus is provided.A phylogenetic b-tubulin tree in conjunction with stromatal HPLC profiles clearly shows that Annulohypoxylon comprises two distinct *** ***/multiforme group might eventually warrant segregation into a new genus as further molecular data become available.

关键词： β-Tubulin biodiversity Chemotaxonomy Phylogeny Systematics Xylariales