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Effects of Feeding Programs Based on One or Two Milk Replacer Daily Meals on Growth, Solid Feed Intake and Rumen Fermentation and Development of Dairy Calves

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Agricultural Sciences 2015年第12期6卷 1428-1440页

作者： christine julien Corine Bayourthe Caroline Lacroux Francis Enjalbert Université de Toulouse INPT ENSAT UMR1388 Génétique Physiologie et Systèmes d’Elevage Castanet-Tolosan France INRA UMR1388 Génétique Physiologie et Systèmes d’Elevage Castanet-Tolosan France UMR INRA ENVT 1225 Interactions H&#244te Agent Pathogène Ecole Nationale Vétérinaire de Toulouse 23 Chemin des Capelles Toulouse France Université de Toulouse INPT ENVT UMR1388 Génétique Physiologie et Systèmes d’Elevage Toulouse France

Sixteen Holstein calves were used to study the effects of two feeding programs (FP) on growth, intake, rumen development and ruminal metabolism from birth to weaning. Two feeding programs based on milk replacer (MR) were tested: a once a day (OAD) MR (200 g/L) distribution vs. a standard twice a day (TAD) MR (125 g/L) distribution. All calves received water, wheat straw and a starter concentrate ad libitum. Four calves per group were slaughtered at weaning and rumen epithelium from the ventral sac was sampled for papillae (RP) density. Results showed that the FP had no effect on body weight of calves and total feed intake. From day 42 to day 56, ruminal pH was lower (P = 0.036) and ruminal oxydo-reducing potential was higher (P = 0.001) in OAD than TAD calves. Ruminal total volatile fatty acid (VFA) concentrations did not significantly differ between FP. From day 21 to day 63, butyrate ruminal concentration was significantly higher in OAD than TAD calves (5.17 vs 3.95 mmol/L). This probably explained the higher development of RP in calves fed once daily. Finally, the tested feeding system based on a once daily MR distribution affects the concentrate feeding pattern of calves.

关键词： Dairy Calves Milk Replacer Feeding Frequency Oxydo-Reducing Potential Rumen Development

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Methodology Article: Can Ruminal Reducing Power Assessed in Batch Cultures be Comparable to <i>in Vivo</i>Measurements?

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Journal of Analytical Sciences, Methods and Instrumentation 2014年第3期4卷 80-86页

作者： christine julien Jean-Philippe Marden Annabelle Troegeler-Meynadier Corine Bayourthe INRA UMR1388 Genetic Physiology and Breeding System Castanet-Tolosan France Lesaffre Feed Additives 282 Avenue de la Marne Marcq-en-Baroeul France

In ruminant field of digestive research, the appeal to methods of less invasive studies and reproducing the in vivo conditions is essential. The objective of the present study was to determine whether the conditions created with the proposed in vitro batch culture was an accurate reproduction of the physico-chemical and fermentative ruminal conditions observed in vivo. Two experiments were conducted to compare ruminal reducing power measured in vitro, i.e. in batch cultures or, in vivo i.e. in live animals: dairy cows at maintenance (Experiment 1) and lactating dairy cows (Experiment 2). In Experiment 1, at the beginning of incubation period, in vitro redox potential (Eh), pH and Clark’s exponent (rH) values were significantly higher than in vivo (+42 mV, +0.25 and +1.9, respectively) whereas volatile fatty acids (VFA) contents were 2.6 fold lower on average. At the end of incubation, Eh, rH values and VFA contents were similar between both methods whereas pH still remained different. In Experiment 2, at the beginning and at the end of incubation period, in vitro Eh, pH and rH values differed significantly than in vivo. As a result, the in vitro method did not provide a tool to evaluate accurately the level of the reducing status of ruminal milieu compared with in vivo measurement. Nonetheless, it provided strong reducing conditions after 8 h of incubation with levels of rH relatively closed to those observed in vivo. In vitro batch culture could be a good alternative to in vivo trials for a screening approach from an ethic and economic point of view in ruminant field of research.

关键词： Batch Cultures Clark’s Exponent Redox Potential

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Field Study of the Impact of Supplementation with Probiotic Yeast(Saccharomyces cerevisiae Sc47-CNCM I-4407)on Reproductive Performance in Dairy Cows

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Agricultural Sciences 2018年第12期9卷 1664-1676页

作者： christine julien Maxime Briche Héloise Legendre Vincent Delcloy Gérard Heumez Phileo Lesaffre Animal Care Marcq-en-BaroeulFrance Gènes Diffusion DouaiFrance

Probiotic yeasts are used widely today in the diets of dairy cows with a high production potential. Various studies have researched the effects of live yeasts, in particular Saccharomyces cerevisiae, on digestion, metabolism and performance in dairy cows. The present study aims at evaluating the beneficial effects of using probiotic yeast in dairy cattle farms as a means of improving the breeding performance of milking cows. A group of 14 dairy cattle farms, with at least 40 dairy cows, was selected for the study, all of them using artificial insemination (AI) and that had not used probiotics in the two milking years preceding the study. On these farms, the lactating cows received probiotic yeast supplementation (5 g/cow/day, Actisaf®, Saccharomyces cerevisiae Sc47-CNCM I-4407, 1.1010 CFU/g, Phileo Lesaffre Animal Care, Marcq-en-Baroeul, France) over 13 months including a 4-week period of adaptation to the product and then the observation period lasting a full year. The analysis was therefore based on 2421 Holstein females inseminated with a total of 4230 doses of conventional Holstein semen at 14 farms over three consecutive years: the period of observation and the two preceding years stated as reference period. Use of daily supplementation with probiotic yeast resulted in a significant improvement of 4 points on average in the success rate of artificial insemination and of 5 points in the success rate of first artificial insemination in multiparous dairy cows. The number of inseminations required to obtain a pregnancy was therefore reduced from 3.1 in the reference period to 2.7 in the probiotic yeast supplementation period (p = 0.007). In parallel, the fat and protein yield by these same animals tended to improve (+5%, p = 0.07) between these two periods, demonstrating that it is possible to combine milk performance and maintenance of an effective reproductive system.

关键词： Probiotic Yeast Dairy Cows Reproduction

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Quantitative Analysis of the Relationship between Ruminal Redox Potential and pH in Dairy Cattle: Influence of Dietary Characteristics

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Agricultural Sciences 2017年第7期8卷 616-630页

作者： Yayu Huang Jean Philippe Marden Chaouki Benchaar christine julien Eric Auclair Corine Bayourthe Physiologie et Systèmes d’ Elevage UMR1388 Génétique Université de Toulouse INPT ENSAT Castanet-Tolosan France Physiologie et Systèmes d’ Elevage UMR1388 Génétique INRA Castanet-Tolosan France Phileo Lesaffre Animal Care Marcq-en-Baroeul France Agriculture and Agri-Food Canada Sherbrooke Research and Development Centre Sherbrooke Canada

The ruminal redox potential (Eh) can reflect the microbiological activity and dynamics of fermentation in the rumen. It might be an important indicator of rumen fermentation in combination with pH. However, the ruminal Eh has been rarely studied in dairy cows due to the difficulty of its measurement, and the relationship between ruminal Eh and pH is not clear. The objective of this study was to investigate the relationship between ruminal Eh and pH of dairy cows by meta-analysis of systematic measurements from different experiments. A database was constructed from 22 experiments on cannulated dairy cattle including 57 dietary treatments. The ruminal pH and Eh were measured without air contact between 0 and 8 h post-feeding. The results demonstrated a quadratic correlation between ruminal Eh and pH with a reliable within-animal variation (Eh = -1697 + 540.7 pH -47.7 pH2, nobservation = 70, nanimal = 26, P Eh, but not always to the same extent. Some of them still influenced the relationship between ruminal Eh and pH. While the mechanism of the interaction between ruminal Eh and pH remains to be elucidated, it would be interesting to associate Eh to microbial profile, ruminal VFA concentration and milk production performance in future studies.

关键词： Redox Potential pH Rumen Diet Dairy Cow

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Interaction between Live Yeast and Dietary Rumen Degradable Protein Level: Effects on Diet Utilization in Early-Lactating Dairy Cows

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Agricultural Sciences 2015年第1期6卷 1-13页

作者： christine julien Jean Philippe Marden Eric Auclair Raymond Moncoulon Laurent Cauquil Jean Louis Peyraud Corine Bayourthe Université de Toulouse INPT ENSAT UMR1388 Génétique Physiologie et Systèmes d’Elevage Castanet-Tolosan France INRA UMR1388 Génétique Physiologie et Systèmes d’Elevage Castanet-Tolosan France Université de Toulouse INPT ENVT UMR1388 Génétique Physiologie et Systèmes d’Elevage Toulouse France Lesaffre Feed Additives Marcq-en-Baroeul France INRA UMR1348 PEGASE F-35590 Saint-Gilles France

Four early lactating Holstein cows were used to study the effect of live yeast (LY, Actisaf®CNCM I-4407, Lesaffre Feed Additives, Marcq en Baroeul, France) supplementation on diet digestive utilization of dairy cows receiving concentrated corn silage-based diets with two rumen-degradable protein (RDP) levels. For a 33 d period, cows were fed a total mixed ration (TMR) containing an adequate level (AL) of RDP or a low level (LL, 30% below AL) by using soybean meal or tanned soybean meal, respectively: for 21 d with no LY addition followed by 12 d during which LY was added to the diet. The pH and redox potential (Eh) were recorded and ruminal fluid samples were collected over 3 consecutive days. Feces were collected individually over 48 h and individual dry matter intake (DMI) was measured for determining apparent nutrient digestibility. The effective degradability of individual feed ingredients composing both diets was evaluated with nylon bags technique. Structure of the ruminal bacterial community was studied and diversity index was calculated. Digestibility of organic matter (OM) and crude protein (CP) were lower for LL than those for AL. With LY, digestibility of OM and CP was increased: +2.4 and +0.8 points, for AL, and +3.7 and +5.9 points for LL, respectively. Live yeast reduced dietary N ruminal degradation with both AL and LL. Ruminal pH and Eh were lower with AL compared to LL: 5.95 and –167 mV vs. 6.13 and –144 mV. Live yeast increased ruminal total volatile fatty acids (VFA) (+8.6%), C2 (+10%), and C4 (+35%) contents for LL and decreased that of C3 (?9.8%) for AL. Neither the structure of bacterial populations of the rumen nor the diversity index (Shannon) was altered by treatments. Those results suggested a specific interest in using LY in RDP deficient diets for early lactating cows.

关键词： Dairy Cow Live Yeast Nitrogen Digestion Ruminally Degradable Protein

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Complete Hydatidiform Mole at Menopause: A Case Report from Panzi Hospital, Bukavu, Democratic Republic of Congo

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Open Journal of Obstetrics and Gynecology 2024年第7期14卷 1010-1017页

作者： julien Bwama De-Joseph Kakisingi Mibi Nsenga Bin Musa Jeff Andrea Mbozi Déborah Kambonesa Salire Dieudonné Kakusu christine Amisi Tina Aroni Toto Tchass Chasinga Baharanyi Denis Mukwege Mukengere Olivier Nyakio Ngeleza Department of Gynaecology-Obstetrics Faculty of Medicine and Community Health Evangelical University in Africa Bukavu Democratic Republic of Congo Gynaecology-Obstetrics Department Panzi General Referral Hospital Bukavu Democratic Republic of Congo Saint Vincent Hospital Bukavu Democratic Republic of Congo Medical Imaging Department Panzi General Referral Hospital Bukavu Democratic Republic of Congo Anatomopathology Department Panzi General Referral Hospital Bukavu Democratic Republic of Congo Panzi Foundation Bukavu Democratic Republic of Congo Department of Gynaecology-Obstetrics Faculty of Medicine Official University of Bukavu Bukavu Democratic Republic of Congo Department of Gynaecology-Obstetrics Faculty of Medicine University of Kindu Kindu Democratic Republic of Congo

Gestational trophoblastic disease is an abnormal proliferation of trophoblastic tissue during pregnancy. It occurs in women of childbearing age, although a few cases have also been observed in post-menopausal women, although it is extremely rare in the latter. Here we describe a rare case of complete hydatidiform mole in a 56-year-old female patient who presented with genital bleeding combined with nausea and vomiting and a gravid uterus 16 cm in height. The ultrasound findings and the increase in serum β-HCG to 182566.00 mIU/ml suggested a diagnosis of complete hydatidiform mole. Given the post-menopausal state and the future risk of post-molar gestational trophoblastic neoplasia, we opted for total hysterectomy without preservation of the adnexa via a transabdominal approach, followed by antimitotic treatment with methotrexate. The uterus measured 18.45 cm × 11.18 cm with intra cavitary vesicles. Microscopic examination showed chorionic villi of variable size and shape, most of which were dilated and oedematous, associated with trophoblastic cell proliferation and haemorrhage suggestive of complete benign hydatidiform mole. Follow-up showed a consistent decrease in serum β-HCG levels and no evidence of residual disease. A suspicion of gestational trophoblastic disease should be borne in mind when evaluating a patient with peri- or post-menopausal bleeding to avoid delay in diagnosis and treatment.

关键词： Complete Hydatidiform Mole Menopause β-HCG Panzi Bukavu DRC

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Polyester-supported Chitosan-Poly(vinylidene fluoride)-Inorganic-Oxide-Nanoparticles Composites with Improved Flame Retardancy and Thermal Stability

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Chinese Journal of Polymer Science 2020年第1期38卷 84-91,I0007页

作者： Ahmed Abed Nabil Bouazizi Stephane Giraud Ahmida El Achari christine Campagne Olivier Thoumire Reddad El Moznine Omar Cherkaoui julien Vieillard Abdelkrim Azzouz ENSAIT GEMTEX-Laboratoire de Genie et Materiaux TextilesF-59000 LilleFrance Laboratory LPMC Faculty of Science El JadidaChouaib Doukkali UniversityEl JadidaMorocco Laboratory REMTEX ESITHRouted'EI Jadidakm8BP 7731-OulfaCasablancaMorocco Normandie Univ. UNIROUENCNRSPBS(UMR 6270)55 rue Saint Germain27000 EvreuxFrance Normandie Univ. UNI ROUENINSA RouenCNRSCOBRA(UMR 6014)55 rue Saint Germain27000 EvreuxFrance Nanoqam Department of ChemistryUniversity of Quebec at MontrealQCCanada

Polyester(PET) was pre-activated by atmospheric air plasma and coated by various inorganic oxide nanoparticles(MOx) such as titanium dioxide(TiO2), zinc oxide(ZnO), and silicon oxide(SiO2), using poly(vinylidene fluoride)(PVDF) and chitosan(CT) as binders. The resulting PET-PVDF-MOx-CT composites were thermally compressed and then characterized by scanning electron microscopy, Fourier infrared spectroscopy, thermal gravimetric analysis, and flame retardancy(FR) ability tests. PET modifications resulted in more thermally stable and less harmful composites with weaker hazardous gas release. This was explained in terms of structure compaction that blocks pyrolysis gas *** incorporation was found to reduce the material susceptibility to oxidation. This judicious procedure also allowed improving flame retardancy ability, by lengthening the combustion delay and slowing the flame propagation. Chitosan also turned out to contribute to a possible synergy with the other polymers present in the synthesized materials. These results provide valuable data that allow understanding the FR phenomena and envisaging low-cost high FR materials from biodegradable raw materials.

关键词： Polyester nonwovens Composite PVDF Flame retardancy Material oxides Chitosan

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Signet-ring cell carcinoma of the stomach: Impact on prognosis and specific therapeutic challenge

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World Journal of Gastroenterology 2015年第40期21卷 11428-11438页

作者： Simon Pernot Thibault Voron Geraldine Perkins christine Lagorce-Pages Anne Berger julien Taieb Department of Gastroenterology and Digestive Oncology Georges-Pompidou European Hospital Department of Digestive Surgery Georges-Pompidou European Hospital Department of Genetic Georges-Pompidou European Hospital Department of Pathology GeorgesPompidou European Hospital

While the incidence of gastric cancer has decreased worldwide in recent decades,the incidence of signetring cell carcinoma(SRCC) is rising. SRCC has a specific epidemiology and oncogenesis and has two forms: early gastric cancer,which can be resected endoscopically in some cases and which has a better outcome than non-SRCC,and advanced gastric cancer,which is generally thought to have a worse prognosis and lower chemosensitivity than non-SRCC. However,the prognosis of SRCC and its chemosensitivity with specific regimens are still controversial as SRCC is not specifically identified in most studies and its poor prognosis may be due to its more advanced stage. It therefore remains unclear if a specific therapeutic strategy is justified,as the benefit of perioperative chemotherapy and the value of taxanebased chemotherapy are unclear. In this review we analyze recent data on the epidemiology,oncogenesis,prognosis and specific therapeutic strategies in both early and advanced SRCC of the stomach and in hereditary diffuse gastric cancer.

关键词： Gastric cancer Signet ring cell carcinoma Diffuse

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Complete Hydatidiform Mole at Menopause: A Case Report from Panzi Hospital, Bukavu,Democratic Republic of Congo

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Open Journal of Obstetrics and Gynecology（妇产科期刊） 2024年第7期 1010页

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FibroMeters:诊断肝纤维化的血液学检测指标组合(英文)

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传染病信息 2013年第3期26卷 134-140页

作者： Paul Calès Jérme Boursier Frédéric Oberti Isabelle Hubert Yves Gallois Marie christine Rousselet Valérie Moal Laurent Macchi Alain Chevailler julien Chaigneau Gilles Hunault HIFIH Laboratory LUNAM University Hepato-gastroenterology department University Hospital Biochemistry Laboratory Hepato-gastroenterology department University Hospital Pathology department University Hospital Hematology Laboratory University Hospital Immunology Laboratory University Hospital

FibroMeters是具有多项特异性的诊断肝纤维化的血液学检测指标组合,其3个主要诊断目标为显著肝纤维化、肝硬化以及肝纤维化的定量,并由专业系统针对不同病因进行调整从而保证获得准确的结果。因此,主要存在 6 种不同的 FibroMeters:即... 详细信息

FibroMeters是具有多项特异性的诊断肝纤维化的血液学检测指标组合,其3个主要诊断目标为显著肝纤维化、肝硬化以及肝纤维化的定量,并由专业系统针对不同病因进行调整从而保证获得准确的结果。因此,主要存在 6 种不同的 FibroMeters:即针对肝纤维化的3种主要病因慢性病毒性肝炎、酒精性肝病和非酒精性脂肪性肝病的肝纤维化分期和肝纤维化定量。以肝组织病理学方法诊断肝纤维化程度进行对照,FibroMeters 表现出非常高的诊断准确率,是惟一能100%正确区分丙型肝炎患者不伴有肝纤维化或伴有肝硬化的检测方法。在适用性方面,FibroMeters 对于显著肝纤维化的 90%预测值高于其他血清学检测方法。使用包括 7 类内容在内的详细的肝纤维化分期方法,87%的丙型肝炎患者可以获得准确的肝纤维化分期。在实际工作条件下,FibroMeters 重复性高于肝组织病理结果,也高于超声弹性检测。因诊断性能表现非常稳定,FibroMeters 在不同的中心都是稳健的检测方法。目前可选择的检测有:针对肝硬化诊断的 CirrhoMeter,在一项丙型肝炎肝硬化患者的队列中其诊断准确率为 93%(受试者工作特征曲线下面积:0.92),阳性预测值为 100%;针对肝纤维化定量检测的QuantiMeter,是惟一能通过无创方法直接量化肝纤维化程度的手段,对于随访肝硬化及其临床相关事件可能特别有用。除HCV 感染外,FibroMeters对HBV或HIV同时感染患者诊断准确率高,尤其是对酒精性肝病或非酒精性脂肪肝患者,FibroMeters有较高的诊断准确率(对于显著纤维化,受试者工作特征曲线下面积分别为0.96和0.94)。

关键词：肝纤维化活组织检查血清学弹性显像技术

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