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Effects of expression of heterogeneous hydrogenase in Enterobacter aerogenes and Escherichia coli on their hydrogen metabolism

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Effects of expression of heterogeneous hydrogenase in Entero...

Two stages power generation test of the hot dry rock exploration and production demonstration project in the Gonghe Basin,northeastern Qinghai-Tibet plateau,China

The 5th Joint China/Japan Chemical Engineering Symposium

作者： Hao Zheng,chong zhang,yuan Lu,Qiheng Lai and Xin-Hui Xing~* Department of Chemical Engineering,Tsinghua University,Beijing 100084,China

Metabolic engineering is becoming an important tool in the alteration of hydrogen metabolism to enhance the hydrogen yield of hydrogen-producing *** this study, heterogenous genes of[NiFe]-hydrogenase HoxEFUYH and[Fe-Fe]hydrogenase from the respective the cyanobacterium synechocystis PCC6803 and Clostridium acetobutylicum were tried to express in Enterobacter aerogenes,*** BL21(DE3) and *** DH5a by constructing artificial synthetic operons,and the effects of the hoterogenous hydrogenase expression on hydrogenproducing pathway were studied by metabolic flux analysis. First,we established an efficient method for constructing the expression system of heterologous hydrogenase by constructing artificial synthetic *** pCOM10 as the expression vector,plasmids of pCOM10-Chox and pCOM10-Mhyd were constructed to achieve the hererologous expression of the[NiFe]-hydrogenase HoxEFUYH from the cyanobacterium synechocystis PCC6803 and the[Fe-Fe]hydrogenase HydA,HydG,HydE and HydF from Clostridium acetobutylicum by constructing artificial synthetic operons,respectively. The expression of the[Fe-Fe]-hydrogenase hydAGEF in *** and *** BL21(DE3) decreased the cellular growth and hydrogen ***,the expression of the[NiFe]-hydrogenase hoxEFUYH in *** DH5αenhanced the Hyield by 43.2%,and reached to 1.89 mol-H·(mol-glucose),which was the highest hydrogen yield in the strain of *** reported so far. Metabolic flux analysis showed that with the expression of[NiFe]-hydrogenase hoxEFUYH, hydrogenase activity was increased by 60%,which enhanced the H production through formate pathway;meanwhile,as the cyanobacterial locus is reportedly capable of suppressing the hydrogen uptake by hydrogenase 1 and hydrogenase 2 in the host cells,the reducing equivalents of the whole cells were increased with the expression of[NiFe]-hydrogenase *** above two factors were presumed to enhance the overall hydrogen yield of *** DH5α(pCOM10-Chox).

关键词： heterogenous hydrogenase metabolic engineering Enterobacter aerogenes Escherichia coli fermetative hydrogen production

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China Geology 2024年第3期7卷 409-421页

作者： Er-yong zhang Dong-guang Wen Gui-ling Wang Xian-peng Jin Lin-you zhang Hai-dong Wu Wen-shi Wang Cheng-ming Ye Wei Weng Kuan Li Jin-sheng Wu Xian-chun Tang chong-yuan zhang Qing-da Feng Sheng Lian Li-sha Hu Gui-lin Zhu Xing-long Xie Bin Wu Dan Wang Xue Niu Zhao-xuan Niu Dong-lin Liu Hui zhang Wen-hao Xu Shu-qing Yao Li Yang Center for Hydrogeology and Environmental Geology Survey China Geological SurveyMinistry of Natural ResourcesTianjin 300304China Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesChina Geological SurveyShijiazhuang 050061China Institute of Exploration Techniques Chinese Academy of Geological SciencesChina Geological SurveyLangfang 065000China Institute of Exploration Engineering China Geological SurveyMinistry of Natural ResourcesBeijing 100083China Institute of Exploration Technology Chinese Academy of Geological SciencesChengdu 611734China Chinese Academy of Geological Sciences China Geological SurveyBeijing 100037China Institute of Geomechanics Chinese Academy of Geological SciencesChina Geological SurveyBeijing 100081China Geoscience Documentation Center China Geological SurveyMinistry of Natural ResourcesBeijing 100083China

The Hot Dry Rock(HDR)is considered as a clean and renewable energy,poised to significantly contribute to the global energy decarbonization *** HDR projects worldwide have accumulated valuable experience in efficient drilling and completion,reservoir construction,and fracture *** 2019,China Geological Survey(CGS)initiated a demonstration project of HDR exploration and production in the Gonghe Basin,aiming to overcome the setbacks faced by HDR *** the ensuing four years,the Gonghe HDR project achieved the first power generation in 2021,followed by the second power generation test in *** establishing the primary well group in the initial phase,two directional wells and one branch well were *** progress was made in successfully constructing the targeted reservoir,realizing inter-well connectivity,power generation and grid connection,implementing of the real-time micro-seismic monitoring.A closed-loop technical validation of the HDR exploration and production was ***,many technical challenges remain in the process of HDR industrialization,such as reservoir fracture network characterization,efficient drilling and completion,multiple fracturing treatment,continuous injection and production,as well as mitigation of induced seismicity and numerical simulation technology.

关键词： Power generation Hot dry rock(HDR) Enhanced geothermal system(EGS) Genesis mechanism Gonghe Basin Directional drilling Reservoir construction Circulation test Induced seismicity Clean energy exploration engineering

Computed tomography enterography-based radiomics for assessing mucosal healing in patients with small bowel Crohn's disease

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World Journal of Gastroenterology 2025年第3期31卷 62-72页

作者： Hao Ding yuan-yuan Fang Wen-Jie Fan Chen-Yu zhang Shao-Fei Wang Jing Hu Wei Han Qiao Mei Department of Gastroenterology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Radiation Oncology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Gastroenterology Suzhou First People's HospitalSuzhou 234099Anhui ProvinceChina

BACKGROUND Mucosal healing(MH)is the major therapeutic target for Crohn's disease(CD).As the most commonly involved intestinal segment,small bowel(SB)assessment is crucial for CD ***,it poses a significant challenge due to its limited accessibility through conventional endoscopic *** To establish a noninvasive radiomic model based on computed tomography enterography(CTE)for MH assessment in SBCD *** Seventy-three patients diagnosed with SBCD were included and divided into a training cohort(n=55)and a test cohort(n=18).Radiomic features were obtained from CTE images to establish a radiomic *** demographics were analysed to establish a clinical model.A radiomic-clinical nomogram was constructed by combining significant clinical and radiomic *** diagnostic efficacy and clinical benefit were evaluated via receiver operating characteristic(ROC)curve analysis and decision curve analysis(DCA),*** Of the 73 patients enrolled,25 patients achieved *** radiomic-clinical nomogram had an area under the ROC curve of 0.961(95%confidence interval:0.886-1.000)in the training cohort and 0.958(0.877-1.000)in the test cohort and provided superior clinical benefit to either the clinical or radiomic models alone,as demonstrated by *** These results indicate that the CTE-based radiomic-clinical nomogram is a promising imaging biomarker for MH and serves as a potential noninvasive alternative to enteroscopy for MH assessment in SBCD patients.

关键词： Crohn’s disease Computed tomography enterography Mucosal healing Nomogram Radiomics

Efficient and Stable Perovskite Solar Cells and Modules Enabled by Tailoring Additive Distribution According to the Film Growth Dynamics

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Nano-Micro Letters 2025年第2期17卷 387-400页

作者： Mengen Ma Cuiling zhang Yujiao Ma Weile Li Yao Wang Shaohang Wu chong Liu Yaohua Mai Institute of New Energy Technology College of Physics&Optoelectronic EngineeringJinan UniversityGuangzhou 510632People’s Republic of China Key Laboratory of New Semiconductors and Devices of Guangdong Higher Education Institutes Jinan UniversityGuangzhou 510632People’s Republic of China Guangdong Mellow Energy Co. LimitedYuanming RoadZhuhai 519075GuangdongPeople’s Republic of China

Gas quenching and vacuum quenching process are widely applied to accelerate solvent volatilization to induce nucleation of perovskites in blade-coating *** this work,we found these two pre-crystallization processes lead to different order of crystallization dynamics within the perovskite thin film,resulting in the differences of additive *** then tailor-designed an additive molecule named 1,3-bis(4-methoxyphenyl)thiourea to obtain films with fewer defects and holes at the buried interface,and prepared perovskite solar cells with a certified efficiency of 23.75%.Furthermore,this work also demonstrates an efficiency of 20.18%for the large-area perovskite solar module(PSM)with an aperture area of 60.84 cm^(2).The PSM possesses remarkable continuous operation stability for maximum power point tracking of T_(90)>1000 h in ambient air.

关键词： Gas quenching Additive distribution Buried passivation Blade coating Crystallization dynamics

The first power generation test of hot dry rock resources exploration and production demonstration project in the Gonghe Basin,Qinghai Province,China

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China Geology 2022年第3期5卷 372-382页

作者： Er-yong zhang Dong-guang Wen Gui-ling Wang Wei-de Yan Wen-shi Wang Cheng-ming Ye Xu-feng Li Huang Wang Xian-chun Tang Wei Weng Kuan Li chong-yuan zhang Ming-xing Liang Hong-bao Luo Han-yue Hu Wei zhang Sen-qi zhang Xian-peng Jin Hai-dong Wu Lin-you zhang Qing-da Feng Jing-yu Xie Dan Wang Yun-chao He Yue-wei Wang Zu-bin Chen Zheng-pu Cheng Wei-feng Luo Yi Yang Hao zhang En-lai Zha Yu-lie Gong Yu Zheng Chang-sheng Jiang Sheng-sheng zhang Xue Niu Hui zhang Li-sha Hu Gui-lin Zhu Wen-hao Xu Zhao-xuan Niu Li Yang Center for Hydrogeology and Environmental Geology Survey China Geological SurveyMinistry of Natural ResourcesBaoding 071051China Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesChina Geological SurveyShijiazhuang 050061China Department of Natural Resources of Qinghai Province Xining 810001China Institute of Exploration Techniques Chinese Academy of Geological SciencesChina Geological SurveyLangfang 065000China Department of Hydrogeology and Environmental Geology China Geological SurveyMinistry of Natural ResourcesBeijing 100037China Chinese Academy of Geological Sciences China Geological SurveyBeijing 100037China Beijing Institute of Exploration Engineering China Geological SurveyMinistry of Natural ResourcesBeijing 100083China Institute of Geomechanics Chinese Academy of Geological SciencesChina Geological SurveyBeijing 100081China Institute of Geophysical and Geochemical Exploration Chinese Academy of Geological SciencesChina Geological SurveyLangfang 065000China Institute of Exploration Technology Chinese Academy of Geological SciencesChina Geological SurveyChengdu 611734China Geoscience Documentation Center China Geological SurveyMinistry of Natural ResourcesBeijing 100083China Jilin University Changchun 130012China Oil and Gas Survey China Geological SurveyMinistry of Natural ResourcesBeijing 100083China Guangzhou Institute of Energy Conversion Chinese Academy of SciencesGuangzhou 510640China Institute of Geophysics China Earthquake AdministrationBeijing 100081China Institute of the Hydrogeology and Engineering Geology of Qinghai Xining 810008China

Hot dry rock(HDR)is a kind of clean energy with significant *** the 1970s,the United States,Japan,France,Australia,and other countries have attempted to conduct several HDR development research projects to extract thermal energy by breaking through key ***,up to now,the development of HDR is still in the research,development,and demonstration *** HDR exploration borehole(with 236℃ at a depth of 3705 m)was drilled into Triassic granite in the Gonghe Basin in northwest China in ***,China Geological Survey(CGS)launched the HDR resources exploration and production demonstration project in *** three years of efforts,a sequence of significant technological breakthroughs have been made,including the genetic model of deep heat sources,directional drilling and well completion in high-temperature hard rock,large-scale reservoir stimulation,reservoir characterization,and productivity evaluation,reservoir connectivity and flow circulation,efficient thermoelectric conversion,monitoring,and geological risk assessment,*** the whole-process technological system for HDR exploration and production has been preliminarily established *** first power generation test was completed in November *** results of this project will provide scientific support for HDR development and utilization in the future.

关键词： Hot dry rock Directional drilling Reservoir stimulation Microseismic monitoring Organic Rankine cycle(ORC) Power generation test Energy geological survey engineering Gonghe Basin Qinghai Province China

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： yuan Zhao Yidan zhang Xin Liu Jian zhang Ya Gao Shuyue Li Cui Chang Xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

Trends of alkaline phosphatase to prealbumin ratio in patients with hepatitis B linked to hepatocellular carcinoma development

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World Journal of Gastroenterology 2025年第2期31卷 42-51页

作者： Wen-chong Zhen Jing Sun Xue-Ting Bai Qian zhang Zi-Han Li Yi-Xin zhang Rong-Xuan Xu Wei Wu Zhi-Han Yao Chun-Wen Pu Xiao-Feng Li School of Public Health Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Graduate School Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Dalian Public Health Clinical Center Dalian Municipal Research Institute for Public HealthDalian 116001Liaoning ProvinceChina Department of Epidemiology and Health Statistics Dalian Medical UniversityDalian 116044Liaoning ProvinceChina

BACKGROUND Chronic hepatitis B often progresses silently toward hepatocellular carcinoma(HCC),a leading cause of mortality *** detection of HCC is crucial,yet *** To investigate the role of dynamic changes in alkaline phosphatase to prealbumin ratio(APR)in hepatitis B progression to *** Data from 4843 patients with hepatitis B(January 2015 to January 2024)were *** incidence rates in males and females were compared using the log-rank *** were evaluated using Kaplan–Meier *** Linear Mixed-Effects Model was applied to track the fluctuation of APR levels over ***,Joint Modeling of Longitudinal and Survival data was employed to investigate the temporal relationship between APR and HCC *** The incidence of HCC was higher in *** ensure the model’s normality assumption,this study applied a logarithmic transformation to APR,yielding *** levels were higher in females(t=5.26,P<0.01).A 1-unit increase in ratio correlated with a 2.005-fold higher risk of HCC in males(95%CI:1.653-2.431)and a 2.273-fold higher risk in females(95%CI:1.620-3.190).CONCLUSION Males are more prone to HCC,while females have higher APR *** no baseline APR link,rising APR indicates a higher HCC risk.

关键词： Alkaline phosphatase to prealbumin ratio Chronic hepatitis B patients Hepatocellular carcinoma Retrospective cohort study Linear Mixed-Effect Mode Joint Modelling of Longitudinal and Survival data

Mechanism of inflammatory response and therapeutic effects of stem cells in ischemic stroke:current evidence and future perspectives

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Neural Regeneration Research 2025年第1期20卷 67-81页

作者： Yubo Wang Tingli yuan Tianjie Lyu Ling zhang Meng Wang Zhiying He Yongjun Wang Zixiao Li Vascular Neurology Department of NeurologyBeijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Shanghai Engineering Research Center of Stem Cells Translational Medicine ShanghaiChina National Center for Healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Institute for Regenerative Medicine Shanghai East HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina Chinese Institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center of Digital Healthcare for Neurological Diseases BeijingChina

Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger ***,in particular,bears a high burden of stroke *** recent years,the inflammatory response after stroke has become a research hotspot:understanding the role of inflammatory response in tissue damage and repair following ischemic stroke is an important direction for its *** review summarizes several major cells involved in the inflammatory response following ischemic stroke,including microglia,neutrophils,monocytes,lymphocytes,and ***,we have also highlighted the recent progress in various treatments for ischemic stroke,particularly in the field of stem cell ***,understanding the complex interactions between inflammation and ischemic stroke can provide valuable insights for developing treatment strategies and improving patient *** cell therapy may potentially become an important component of ischemic stroke treatment.

关键词： cell therapy immune cell inflammatory ischemic stroke stem cell

Study on the hydrogen absorption properties of a YGdTbDyHo rare-earth high-entropy alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 127-135页

作者： Tongyue Li Ziliang Xie Wenjiao Zhou Huan Tong Dawen Yang Anjia zhang yuan Wu Xiping Song State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China Whole Win(Beijing)Materials Sci.&Tech.Co. Ltd.Beijing 100083China

This study investigated the microstructure and hydrogen absorption properties of a rare-earth high-entropy alloy(HEA),*** indicated that the YGdTbDyHo alloy had a microstructure of equiaxed grains,with the alloy elements distributed *** hydrogen absorption,the phase structure of the HEA changed from a solid solution with an hexagonal-close-packed(HCP)structure to a high-entropy hydride with an faced-centered-cubic(FCC)structure without any secondary phase *** alloy demonstrated a maximum hydrogen storage capacity of 2.33 H/M(hydrogen atom/metal atom)at 723 K,with an enthalpy change(ΔH)of-141.09 kJ·mol^(-1)and an entropy change(ΔS)of-119.14 J·mol^(-1)·K^(-1).The kinetic mechanism of hydrogen absorption was hydride nucleation and growth,with an apparent activation energy(E_(a))of 20.90 kJ·mol^(-1).Without any activation,the YGdTbDyHo alloy could absorb hydrogen quickly(180 s at 923 K)with nearly no incubation period *** reason for the obtained value of 2.33 H/M was that the hydrogen atoms occupied both tetrahedral and octahedral *** results demonstrate the potential application of HEAs as a high-capacity hydrogen storage material with a large H/M ratio,which can be used in the deuterium storage field.

关键词： rare-earth high-entropy alloy hydrogen absorption capacity pressure–composition–temperature curves kinetics