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微生物学通报 2009年第3期36卷 334-338页

作者：谌书刘从强连宾中国科学院地球化学研究所环境地球化学国家重点实验室贵州贵阳550002 西南科技大学固体废物处理与资源化省部共建教育部重点实验室四川绵阳621010

本文研究对磷矿石有风化作用的胶质芽胞杆菌(Bacillus muscilaginosus)在风化磷矿石过程中菌体蛋白质组的变化及差异,以此了解该风化作用的分子生物学机制。将该菌接入液体培养基中,加磷矿粉为处理组,不加磷矿粉为对照组,静置培养。培养... 详细信息

本文研究对磷矿石有风化作用的胶质芽胞杆菌(Bacillus muscilaginosus)在风化磷矿石过程中菌体蛋白质组的变化及差异,以此了解该风化作用的分子生物学机制。将该菌接入液体培养基中,加磷矿粉为处理组,不加磷矿粉为对照组,静置培养。培养10d后,取上清液离心后的固体物和部分上清液中的外分泌蛋白用作菌体蛋白质组学分析。菌体蛋白质组二维电泳图谱进行比较和软件分析后,处理组可见蛋白点数为(1134±98)个,对照组可见蛋白点数为(729±53)个,两者比较具有统计学差异(P214个蛋白点表达上调,75个蛋白点表达下调,207个蛋白质点为新合成。细菌对磷矿石风化作用时激活了某些蛋白的表达,新合成、表达量增加和表达量下降的蛋白质点与细胞的代谢调控、细胞激活、增生、分化和信号转导通路等有关;揭示了该菌受矿粉的影响及其适应环境的分子生物学机制。

关键词：胶质芽胞杆菌磷矿石微生物风化蛋白质组

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Removal of Strontium from Aqueous Solution by Diatomite

Removal of Strontium from Aqueous Solution by Diatomite

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Wei ZHANG1 Faqin DONG2 * Bo WU3 Huanqing SONG4 Hailiang DONG5 1 Analytical and Testing Center Southwest University of Science and Technology Mianyang china 2 key laboratory of solid waste treatment and resource recycle Ministry of Education Mianyang china 3 School of National Defense Science and Technology Southwest University of Science and Technology Mianyang china 4 School of Environment and resource Southwest University of Science and Technology Mianyang china 5 Department of Geology Miami University Oxford U.S.A

Diatomite was investigated as an adsorbent to remove strontium （II） from aqueous solution. The results demonstrate that diatomite can be successfully used as an adsorbent for the removal of heavy metal/ radionuclide ions from aqueous solution. PH value of the solution was an important factor for both solution chemistry of metals and adsorbent. The adsorption capacity of diatomite with strontium ions was better in acid condition. The removal rate rose as the dosage of diatomite increased, and declined as the concentration of strontium ions increased. While, the adsorption capacity declined as the dosage of diatomite increased, and rose as the concentration of strontium ions increased. The kinetics research indicated that the adsorption of strontium ions on diatomite was a rapid process. The removal rate and adsorption capacity could reach 95% of adsorption equilibrium in 60 minutes. The experimental results were fitted well to PSO kinetics model.

关键词： diatomite strontium ions adsorption PSO model

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Study on Preparation and Adsorbability of Sewage Sludge-based Activated Carbon

Study on Preparation and Adsorbability of Sewage Sludge-base...

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Lijun YANG * Qunwei DAI key laboratory of solid waste treatment and resource recycle Ministry of Education Mianyang 621010 china

The techniques which preparing for activated carbon from sewage sludge through microwave radiatio n in existence of H3PO4 was researched in the paper. Th e effect of microwave power, radiation time and H3PO4 solution concentration was investigated. It was showed t hat optimized conditions on preparation of Sewage Slud ge-based Activated Carbon （ACSS） were microwave po wer 480W, radiation time 260s, the H3PO4 solution conc entration 35%～40%. On the condition, the iodine value of ACSS was 506mg/g, BET surface area was 192m2/g, t he pore volume was 0.30ml/g, and the average pore diam eter was 6.25nm. The ACSS was used to treat secondary effluent from sewage treatment plant. The effect of ACS S’ dosage on the adsorption efficiency was studied. The Freundlich model and Langmuir model were all fit for t he wastewater adsorption.

关键词： sewage sludge-based activated carbon (ACSS) microwave adsorption

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Comparative Experimental Research on Preparation of Activated Carbon from Three Different Kinds of Agricultural wastes

Comparative Experimental Research on Preparation of Activate...

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Hui JIANG 1 2 1 key laboratory of solid waste treatment and resource recycle Ministry of Education Mianyang china 2 School of Environment and resource Southwest University of Science and Technology Mianyang china

Comparative experimental research on technology of preparing activated carbon from three different kinds of agricultural wastes was studied. The elemental composition of raw materials and experimental samples was analyzed, and the samples were observed by SEM and compared with commercial activated carbon. It was found that cotton stalks, corn stalk and rice stalk could be converted into activated carbon by activation method, the adsorption ability of cotton stalk activated carbon and corn stalk activated carbon are relatively close to each other, and the cotton stalk and corn stalk are more suita- ble for the production of activated carbon.

关键词： Comparative experimental research agricultural wastes activated carbon

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Research on Leaching Silver from waste PDP Glasses

Research on Leaching Silver from Waste PDP Glasses

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Pengfei JIANG1, Mengjun CHEN1*, Haiyan CHEN1 1 key laboratory of solid waste treatment and resource recycle, Ministry of Education, Mianyan, china

In this study, nitric acid leaching process was adopted to extract silver (Ag) from waste PDP glass. The effect of particle size, HNO3 concentration, liquid-to-solid ratio(L/S) and reaction time on silver leaching rate was studied by orthogonal tests and the optimum leaching conditions for Front panel (FP)were obtained when the particle size was 1 mm, HNO3 was 3 M, L/S was 1 and reaction time was 24 h. While the optimum leaching conditions for Back panel (BP) were 50 mm, 3 M, 2 and 24 h. Under the optimum conditions, silver leaching rate of FP and BP were up to 97.7% and 97.9%, respectively.

关键词： waste PDP glass acid leaching Ag orthogonal tests

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Release of volatile organic compounds during bio-drying of municipal solid waste

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Journal of Environmental Sciences 2010年第5期22卷 752-759页

作者： Pinjing He,Jiafu Tang,Dongqing Zhang,Yang Zeng,Liming ShaoState key laboratory of Pollution Control and resource Reuse,key laboratory of Yangtze River Water Environment,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,china. State key laboratory of Pollution Control and resource Reuse Key Laboratory of Yangtze River Water Environment College of Environmental Science and Engineering Tongji University Shanghai 200092 China

Three treatments were tested to investigate the release concentrations of volatile organic compounds （VOCs） during the bio-drying of municipal solid waste （MSW） by the aerobic and combined hydrolytic-aerobic *** showed that VOCs were largely released in the first 4 days of bio-drying and the dominant components were：dimethyl disulfide,dimethyl sulfide,benzene,2-butanone,limonene and methylene ***,the combined hydrolytic-aerobic process was suggested for MSW bio-drying due to fewer aeration quantities in this phase when compared with the aerobic process,and the treatment strategies should base on the key properties of these prominent *** sulfur compounds and terpenes were mainly released in the early phase of bio-drying,whereas,two peaks of release concentrations appeared for aromatics and ketones during ***,for the combined hydrolytic-aerobic processes there were also high concentrations of released aromatics in the shift from hydrolytic to aerobic *** concentrations of released chlorinateds were observed in the later *** the VOCs produced during MSW bio-drying,i.e.,malodorous sulfur compounds,terpenes and chlorinateds,their release concentrations were mainly determined by production rates;for the VOCs presented initially in MSW,such as aromatics,their transfer and transport in MSW mainly determined the release concentrations.

关键词： bio-drying municipal solid waste odors release concentration volatile organic compounds

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Effect of Low-voltage Static Electric Field on Growth of Esoherichia coli

Effect of Low-voltage Static Electric Field on Growth of Eso...

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Qunwei DAI 1*, Faqin DONG 1, Yong WANG 2, Jianjun DENG 2 1 key laboratory of solid waste treatment and resource recycle, Ministry of Education, Mianyang, china 2 The Fourth People’s Hospital of Mianyang City, Mianyang, 621000, china

Study the influence of different static electric field intensity on the growth of Esoherichia coli. The static electric field of 3V and 24V were put on the big testing tubes ( Ф= 30mm) that were used to culture E. coli. Measure the absorbency and bacterial colony numbers in the growth process of E. coli. At the same time, the shapes of E. coli under the action of 24V static electric field was observed and contrasted between testing tube and control tube through scanning electricity mirror (SEM) analysis. The growth of E. coli had been restrained during cultured- prophase under the experiment conditions. Stabilization growth period of E. coli was advanced respectively 2 hours and 4 hours under the action of static electric fields of 3V and 24V. E. coli appeared the second logarithm growth after the first stabilization period began 6h. That is growth remediation of bacterial thalli. And in the experimental conditions, the intensity of static electric field is in inverse ratio to the restoring speed. In addition, the thalli distortion of E. coli had been found under the experiment condition through Scanning electron microscope (SEM) photos. These can further show that the external low-voltage static electric field has some extent effect on the growth of E. coli. The low-voltage static electric field has restraint to the growth of E. coli.

关键词： static electric field Esoherichia coli restraint growth remediation

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Comprehensive Utilization of Copper Tailings in Sichuan Province

Comprehensive Utilization of Copper Tailings in Sichuan Prov...

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Aiping LIU1 Qiming FENG1 * Weiqing WANG2 Lili HE1 Bolian ZHANG1 1key laboratory of waste solid treatment and resource recycle of Ministry of Education Mianyang Sichuan china 2 School of environmental engineering and resources Southwest University of Science and Technology Mianyang china

The chemical composition, mineral composition, relative content, granularity and granularity distribution of the copper tailings in Sichuan were tested and studied. The tailings shale solid bricks were prepared by adding the tailings to shale as different ratio, and the effect of different ratio of materials and firing system on the compression strength of the products was studied. Use the tailings as fine aggregate, cement as cementing material, lime as activator and concrete foaming agent as pre-pore agent, cast molding, and then cure 28d. The compression strength and density of the products were tested, and the effect of different ratio of materials and curing condition on properties of lightweight non-sintered was studied. The results indicated when the addition amount of tailings was 25% to 45%, sintered temperature was 950℃ to 1050℃ and thermal insulation time was 3h, the compression strength of the bricks reached to 14.4Mpa to 40.1Mpa, water absorptivity was 8.7% to 13.9%, which have reached to the national startand of sintered shale solid brick （GB5101-2003）. When the addition amount of tailings was 70% to 80%, the dry volume weight of the lightweight non-sintered brick was equivalent to 2/3 of shale solid brick, the compression strength reached to 5.4Mpa to 10.4Mpa, the bricks could be applicable to use filling block of building loading and non-loading, and belong to low-energy environmental friendly wall material.

关键词： Mircoorganism Magnetic sorbent Crude oil spill Cleanup Biomorphic mineralization

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Study on Screening and Fermentation Conditions of Xylanase-producing Microorganism from the Biological waste

Study on Screening and Fermentation Conditions of Xylanase-p...

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The 3rd International Conference on Environmental Technology and Knowledge Transfer

作者： LIU Bin WU Ke YU Zhimin JIN Jie (Engineering Technology Research Center of Municipal solid waste treatment and resource Application Anhui Province Hefei University key laboratory of Applied Enzymology & Engineering Hefei 230022 china)

A xylanase production strain was isolated from biological *** can use bran,corn cob produced *** the single factor test to xylose and NH4NO3 as C,N source,the strain can produce *** design test results showed that the appropriate conditions for enzyme production:xylose 60g/L,NH4NO3 2.5g/***-80 1.5g/L,KH2PO4 5.0g/L,pH 5.0,1mL （2.0×107） spore suspension inoculums,28℃,140r/min,oscillation incubated for *** such condition,the enzyme activity can reach 26.31IU/*** xylanase showed an optimum temperature and pH of 50℃ and 4.6,*** 40℃ relatively stable,activity remained at 97%.The temperature of remaining half xylanase activity after incubated 1h was 53.3℃.

关键词： xylanase isolated fermentation biological waste

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Prepartation Microorganism Composited Magnetic Sorbent for Cleaning Marine Crude Oil Spills

Prepartation Microorganism Composited Magnetic Sorbent for C...

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2011 IEEE International Conference on waste Recycling,Ecology and Environment(ICWREE 2011)

作者： Shiyong SUN1 2* Faqin DONG1 Jia LUO3 Tingting HUO1 Lizhu LIU1 1key laboratory of waste solid treatment and resource recycle of Ministry of Education Mianyang Sichuan china 2School of Environment and resource Southwest University of Science and Technology Mianyang Sichuan china 3 Department of Chemistry Monmouth University 400 Cedar Avenue West Long Branch NJ USA

the objective of this study is preparing a novel environmental friendly light weight magnetic sorbent consisting of bacteria and cobalt by chemical process to further cleaning crude oil spills in sea. The observations by ESEM indicated that a thin granular layer covered on the surface of model microorganism of Staphylococcus epidermildis which provided host for absorbing cobalt ions and depositing cobalt particulates. The results showed that composite present flower-like microspheres with large-scale yields. The high temperature treatment of amorphous bacteria composited cobalt microspheres showed that it well crystallized corresponding to Co3O4 at 800 °C. The microorganism composited magnetic sorbent opens an important alternation absorbent for timely oil spill cleanup.

关键词： Mircoorganism Magnetic sorbent Crude oil spill Cleanup Biomorphic mineralization

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