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Journal of Systematics and Evolution 2009年第4期47卷 311-320页

作者： ~(1,2)Chen-Ying WANG ~1Jian-Cheng ZHAO~* 1(College of life science,Hebei Normal University,Shijiazhuang 050016,china) 2(department of life science,Zhengzhou Teachers College,Zhengzhou 450044,china) (College of life science Hebei Normal University Shijiazhuang 050016 China) (department of life science Zhengzhou Teachers College Zhengzhou 450044 China)

The phylogeny of Ptychostomum was first undertaken based on analysis of the internal transcribed spacer(ITS)region of the nuclear ribosomal(nr)DNA and by combining data from nrDNA ITS and chloroplast DNA rps4 *** parsimony,maximum likelihood,and Bayesian analyses all support the conclusion that the reinstated genus Ptychostomum is not *** funkii(Schw(a|¨)gr.)*** (≡Bryum funkii Schw(a|¨)gr.)is placed within a clade containing the type species of Bryum,*** Hedw. The remaining members of Ptychostomum investigated in the present study constitute another well-supported clade. The results are congruent with previous molecular *** the basis of phylogenetic evidence,we agree with transferring *** Mü***.(≡***(Brid.)Turton≡Bryum archangelicum Bruch & Schimp.), Bryum lonchocaulon Mü***.,Bryum pallescens *** Schw(a|¨)gr.,and Bryum pallens *** Ptychostomum.

关键词： Bryum molecular phylogeny nuclear ribosomal DNA internal transcribed spacer sequences Ptychostomum rps4 sequences.

Microsporogenesis and meiotic behavior in nine species of the genus Pinus

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Journal of Systematics and Evolution 2009年第4期47卷 287-290页

作者： ~(1,2)Hui-Sheng DENG ~3Da-Ming ZHANG~* ~(1,3)De-Yuan HONG ~1Cheng-Xin FU 1(College of life science,Zhejiang University,Hangzhou 310029,china) 2(department of Chemistry and life science,Liuzhou Teachers College,Liuzhou 545003,china) 3(State Key Laboratory of Svstematic and Evolutionary Botany,Institute of Botany,Chinese Academy of sciences,Beijing 100093,china) (College of life science Zhejiang University Hangzhou 310029 China) (department of Chemistry and life science Liuzhou Teachers College Liuzhou 545003 China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China)

The meiotic behavior of 10 taxa(nine species and one variety)of the genus Pinus was investigated using pollen mother cells(PMCs)to reveal the differentiation among *** spreads were prepared by conventional *** meiotic index and the average configuration were higher,whereas the frequency of aberrance(chromosomal bridges,fragments,or micronuclei)was lower,in all 10 taxa compared with other *** meiotic index,average configuration,and frequency of irregularity were found to be uniform among the *** was shown that the genomes of the Pinus species investigated were highly stable,confirming results of previous mitotic analyses in this ***,slight differentiation of homologous chromosomes among genomes was revealed by analysis of meiotic configurations in Pinus nigra *** were observed in 9.31%of PMCs in this *** is the first time that quadrivalents have been observed in gymnosperms.

关键词： homologous chromosomes irregularity meioses Pinus pollen mother cell quadrivalent.

Surface investigation on Upilex-S polymide film iradiated by vacuum ultraviolet radiation

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Rare Metals 2007年第S1期26卷 168-174页

作者： LIU Yuyan1),GONG Xianyun2),WAN Zhimin3),and DU Xingwen4)1) department of Applied Chemistry,Harbin Institute of Technology,Harbin 150001,china 2) department of life science and Chemistry,Harbin University,Harbin 150001,china 3) Analysis and Measurement Center,Harbin Institute of Technology,Harbin 150001,china 4) Center for Composite Materials,Harbin Institute of Technology,Harbin 150001,china Harbin Inst Technol Dept Appl Chem Harbin 150001 Peoples R China Harbin Univ Dept Life Sci & Chem Harbin 150001 Peoples R China Harbin Inst Technol Ctr Anal & Measurement Harbin 150001 Peoples R China Harbin Inst Technol Ctr Composite Mat Harbin 150001 Peoples R China

Vacuum ultraviolet radiation of Upilex-S polyimide film 25 μm thick was performed using a gas jet type of vacuum ultraviolet simulator which gives unfixed wavelength from 5 nm to 200 *** redeposited on the films were observed and the nanoparticles formed on the surface consist mainly of carbon *** in the composition and the chemical characteristics of film surface after radiation were identified by X-ray photoelectron spectroscopy(XPS).Relative O content in the radiated area was found to be higher,while N content was lower than in the pristine *** indicates that Upilex-S polyimide film releases N atoms as volatile *** the C1s,O1s envelopes were fitted using Multipak Spectrum *** surface morphologies on iradiated area were examined by Atomic Force Microscope(AFM).Pieces of radiant products protrude from the eradiated surface,leading to considerable roughness for the iradiated *** the surface transmittance of Upilex-S film after radiation reduces.

关键词： vacuum ultraviolet radiation Upilex-S polyimide film

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A Comparative Analysis of B Chromosomes and Genetic Diversity in Maize (Zea mays L.) Landraces from Southwest china

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Journal of Integrative Agriculture 2007年第10期6卷 1166-1172页

作者： YAO Qi-lun1, 2, YANG Ke-cheng1, PAN Guang-tang1 and RONG Ting-zhao1 1 Maize Research Institute, Sichuan Agricultural University, Yaan 625014, P.R.china 2 department of life science, Yangtze Normal University, Fuling 408003, P.R.china Maize Research Institute Sichuan Agricultural University Yaan 625014 P.R.China department of life science Yangtze Normal University Fuling 408003 P.R.Chin

The number of B chromosomes (Bs) in 54 maize landraces from Southwest china was tested by means of cytological observations. Nine landraces with Bs were observed. A map, showing the geographic distribution of the landraces with Bs, was plotted. It was found that southeastern Sichuan Province in china was the main distribution area of the landraces with Bs in Southwest china. In order to obtain information on relationships between Bs and genetic variation, genetic diversity both among and within 11 landraces was evaluated. For each SSR marker, the number of alleles ranged from 3 to 12 with an average of 7.86, which revealed a high level of genetic diversity among maize landraces in Southwest china. Based on SSRs data, higher genetic variation was found in the landraces with 2B, and the genetic distance between the landraces with and without Bs was higher. The results together with the principal component analysis (PCA) supported the hypothesis that maize landraces in Southwest china were first introduced to the middle part of southwest Sichuan, china. At the same time, the effect of Bs on genetic variation was discussed.

关键词： Bs genetic diversity maize landraces SSR markers

The Effects of Low Phosphorus Stress on Morphological and Physiological Characteristics of Maize (Zea mays L.) Landraces

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Journal of Integrative Agriculture 2007年第5期6卷 559-566页

作者： YAO Qi-lun1, 2, YANG Ke-cheng1, PAN Guang-tang1 and RONG Ting-zhao1 1 Maize Research Institute, Sichuan Agricultural University,Yaan 625014, P.R.china 2 department of life science, Changjiang Normal College, Fuling 408003, P.R.china Maize Research Institute Sichuan Agricultural University Yaan 625014 P.R. China department of life science Changjiang Normal College Fuling 408003 P.R. China

A field trial was conducted to investigate main morphological and physiological changes of different maize landraces to low-P stress at the stage of seedling. P-deficiency significantly decreased root volume, total leaf area, and plant dry weight, but greatly increased density of root hairs and root top ratio. In addition, P-deficiency induced the significant enhancement of phosphorus utilization efficiency and the amount of proline, malondialdehye (MDA), acid phosphatase (APase), peroxidase (POD) and superoxide dismutase (SOD), but the significant reduction of P uptake and soluable protein content. Since P-deficiency had smaller effects on the P-tolerant maize landraces DP-44, DP-32 and DP-33 as compared with P-sensitive landraces DP-29 and DP-24, it was demonstrated that differences of tolerance to P-deficiency existed among different maize landraces. The results based on the correlation analysis showed that the economic yield of maize landraces had relationships with their morphological and physiological characteristics under P-deficiency.

关键词： maize landraces P-deficiency low-P stress morphology physiology

SIP30 in the anterior cingulated cortex contributes to the induction of pain-related negative affect in rat

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SIP30 in the anterior cingulated cortex contributes to the i...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Mei HAN~1 Yan ZHANG~1 Ru-Yi HUANG~2 Zhi-Qi ZHAO~1 Yu-Qiu ZHANG~(1 *) 1 Institutes of Neurobiology Institutes of Brain science and State Key Laboratory of Medical Neurobiology Fudan University Shanghai 200032 china 2 department of life science Fudan University Shanghai 200433 china

＜正＞Objective SNARE complex,including SNAβ-25,syntaxin and VAMP,plays an important role in presynaptic neurotransmitter ***β25 interacting protein of 30kD（SIP30）that was first identified in the cochlear hair cells of guinea pig organ of Corti is expressed in many organs but is most abundant in ***,its functional significance remains *** evidence demonstrates that the anterior cingulated cortex（ACC） is implicated in the affective response to noxious *** present study is to investigate whether SEP30 in the ACC play roles in pain-related negative affect in rat and its possible *** The localization of SEP30 was detected by *** chronic constriction injury（CCI） of sciatic nerve was used for neuropathic pain *** escape/avoidance paradigm （PEAβ） test was used to reflect the pain-related negative *** neural processing relating learning and memory was tested by conditioned place avoidance（CPA） induced by electric foot-shock or *** behavioral pain hypersensitivities were measured by Von Frey test for mechanical allodynia and Hargreves’test for thermal ***-ACC microinjection of SIP30 small interfering（siRNA） by letivirus as vector was used for knockdown the expression of SIP30. The efficiency of SIP30 knockdown in the ACC was tested by Western Blot and ***（1） SIP30 especially expressed on neuron not astrocyte or microglia in the rACC.（2） In the PEAβtest,the percentage of time spent in the light side of the chamber in the CCI group was much higher than that of sham group,which means PEA has occurred. （3） Compared with sham CCI group,SIP30 significantly upregulated in the rACC after PEAβtest in CCI group.（4） SIP30 knockdown in the rACC had no effect on CCI-induced pain hypersensitivities and innocuous stimuli-induced CPA.（5） Following SIP30 siRNA microinjected into the rACC,place escape/avoidance behavior to a noxious mechanical stimuli was attenua

关键词： SIP30 pain-related negative affect rACC rat

STUDY ON BIODEGRADABILITY OF POLY (3-HYDROXYBUTYRATE-co-3-HYDROXYVALERATE)/ORGANOPHILIC MONTMORILLONITE NANOCOMPOSITES

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Chinese Journal of Reactive Polymers 2004年第Z1期 65-73页

作者： WANG Shufang1 SONG Cunjiang2,** CHEN Guangxin1 LIU Jing2 YANG Chao2 ZHANG Xihui2 GUO Tianying1 ZHANG Banghua1 1 State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, N & T Joint Academy, Nankai University, Tianjin 300071, china 2 department of Microbiology, life science College, Nankai University, Tianjin 300071, china

Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/Organophilic montmorillonite (PHBV/OMMT) nanocomposites were prepared and the biodegradability of the PHBV/OMMT nanocomposites was studied by a cultivation degrading method in soil suspension. The relationship between structure and biodegradability of PHBV/OMMT nanocomposites was investigated. The results showed that the biodegradability of PHBV/OMMT nanocomposites decreased with increasing amount of OMMT and it was related to the number of PHBV degrading microorganisms in degradation environment, the anti-microbial property of OMMT and the degree of crystallinity of the nanocomposites.

关键词： Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Organophilic montmorillonite Biodegradability.

The role of neuroglobin and cytoglobin against oxidative stress-induced toxicity and hypoxia-induced death in Caenorhabditis elegans models

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The role of neuroglobin and cytoglobin against oxidative str...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Chang-Hong REN~1 Na ZHAO~(1 2) Wei-Guang LI~1 Ji-Ye ZHANG~(1 2) Yan GAO~1 Yong-Hong Wu~1 Cheng-Gang ZHANG~(1 *) 1 Beijing Institute of Radiation Medicine State Key Laboratory of Proteomics Beijing 100850 china 2 department of Biology College of life science Northwest Agriculture and Forestry University Yangling 712100 china

＜正＞Objective Neuroglobin（NGB） and cytoglobin（CGB） are newly discovered members of the oxygen-binding globin superfamily,which are expressed in neuronal tissues and many other tissues,*** difference expression patterns between NGB and CGB suggest that the two globins may play different *** investigate whether the these two globins expressed in neurons play distinct neuronal-specific functions,we focused on a simple organism model, ***,to shed light on the protective roles of the globins in *** *** overexpressing human NGB（hNGB） or CGB（hCGB） in the entire nervous system under the control of an unc-119 promoter were *** protective roles of the two globins in neurons were identified by paraquat sensitivity assay and hypoxic sensitivity assay. Results（1） human NGB transgenic worms showed paraquat *** contrast,hCGB transgenic worms showed similar paraquat sensitivity to control worms.（2） Overexpression of human NGB in *** can rescue the paraquat sensitive phenotype of mutation tm2825 strain of *** gene gib-13,which displayed sequence similarity to human neuroglobin.（3） Human NGB/human CGB transgenic worms failed to suppress hypoxia-induced death.（4） The mutation strain（tm2825） displayed hypoxia *** The two globins expressed in neurons play distinct neuronspecific function in *** *** transgenic worms showed paraquat-resistant phenotype but not hypoxia resistance,which is important for us to understand the relationship between reactive oxygen species（ROS） and hypoxia injury.

关键词： human neuroglobin human cytoglobin transgenic C.elegans hypoxia ROS

Viral Specific Cytotoxic T Cells Inhibit the Growth of TfR- expressing Tumor Cells with Antibody Targeted viral peptide/HLA-A2 complexes

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Viral Specific Cytotoxic T Cells Inhibit the Growth of TfR- ...

第六届全国免疫学学术大会

作者： Jianan Li~(1,2) Xiufang Weng~1 Zhihui Liang~1 Maohua Zhong~1 Xueling Chen~1 Shenjun Lu~1 Wei Sun~1 Yinhong Song~1 Yali Zeng~1 Qing Li~1 Xiongwen WU 1.department of Immunology,Tongji Medical College,Huazhong University of science & Technology,Wuhan 430030,china 2.department ofbiotechnolog,life science College,Jianghan University,Wuhan 430056,china

Background:A cancer immunotherapy strategy is described herein that redirects cytotoxic T cells(CTLs)of viral specificity to tumor cells by targeting viral peptide/HLA-A2 complexes to tumor cells via antibody delivery...

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Different response patterns of local field potentials of the olfactory bulb in awake and anesthetized rats

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Different response patterns of local field potentials of the...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： An-An LI~1,Min LIU~2,Lei ZHANG~2,Qing LIU~1,Ling GONG~1,Fu-Qiang XU~(1,3,*) 1 Wuhan MRC,State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Institute of Physics and Mathematics,the Chinese Academy of sciences,Wuhan 430071,china 2 department of Chemistry and life science,Hubei University of Education,Wuhan 430205,china 3 Wuhan National Laboratory for Optoelectronics,Wuhan 430074,china

＜正＞The olfactory bulb（OB）,an important odor information processing center in olfactory system,not only has heavy intermutual neural projections with brain areas related to olfaction,including piriform cortex,amygdala, hippocampus and striatum,but also receives regulatory affections from other brain regions such as raphe nuclei and locus *** of these projections provide the possible modulation of the activity in the OB by brain *** awake and anesthetized states are two different brain conditions and are both used extensively in electrophysiological studies of ***,the activity patterns of OB local field potentials（LFP）in responding to odor stimulation at different brain states are *** clarify this question,the LFP oscillations at granule cell layer of the OB in awake and anesthetized animals were *** found that the LFP responses induced by odor in awake state were complex,with different frequency bands showed different patterns:13-30 Hz,increased significantly;30-60 Hz,almost no response;and 60-90 Hz,decreased *** addition,the increased response of 13-30 Hz was transient, whereas the decreased response of 60-90 Hz was ***,when the animals were anesthetized by chloral hydrate,all of the three frequency bands showed significantly increased and long-lasting ***, the activity patterns of OB LFP in responding to odor stimulation are significantly different when the animals are under awake and anesthetized states,reflecting different neural mechanisms involved in the odor information processing under the two brain states.

关键词： brain states local field potential olfactory bulb odor