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Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens

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Advanced Photonics 2019年第1期1卷 45-57页

作者： Peng Fei Jun Nie Juhyun lee Yichen Ding Shuoran Li hao Zhang Masaya Hagiwara Tingting Yu Tatiana Segura chih-Ming Ho Dan Zhu Tzung KHsiaic Huazhong University of Science and Technology School of Optical and Electronic InformationWuhanChina Huazhong University of Science and Technology Britton Chance Center for Biomedical PhotonicsWuhan National Laboratory for OptoelectronicsWuhanChina University of California Los AngelesDepartment of BioengineeringLos AngelesCaliforniaUnited States University of Texas at Arlington Joint Department of Bioengineering of UT Arlington/UT SouthwesternArlingtonTexasUnited States University of California Los AngelesSchool of MedicineLos AngelesCaliforniaUnited States University of California Los AngelesChemical and Biomolecular Engineering DepartmentLos AngelesCaliforniaUnited States Osaka Prefecture University Nanoscience and Nanotechnology Research CenterResearch Organization for the 21st CenturyOsakaJapan University of California Los AngelesMechanical and Aerospace Engineering DepartmentLos AngelesCaliforniaUnited States

A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution.A nonaxial,continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional(3-D)nanoscale shifts ***,by adopting a subvoxel-resolving procedure,the SLSM method models these low-resolution,cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the *** technique can surpass the optical limit of a conventional light-sheet microscope by more than three times,with high acquisition speeds of gigavoxels per *** fast reconstruction of 3-D cultured cells,intact organs,and live embryos,SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue(>100 mm3)and observing single cell(∼1-μm resolution).It also eliminates the need of complicated mechanical stitching or modulated illumination,using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput,high-resolution 3-D microscopy,which could be tailored for a wide range of biomedical applications in pathology,histology,neuroscience,etc.

关键词： light-sheet microscopy subvoxel-resolving reconstruction large tissue imaging high-throughput volumetric imaging

基于高光谱成像技术应用光谱及纹理特征识别柑橘黄龙病（英文）

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光谱学与光谱分析 2016年第7期36卷 2344-2350页

作者：马淏吉海彦 Won Suk lee 中国农业大学现代精细农业系统集成研究教育部重点实验室北京100083 河南科技大学农业工程学院河南洛阳471003 Department of Agricultural and Biological Engineering University of FloridaGainesvilleFL32611USA

讨论了基于高光谱成像技术光谱及纹理特征在识别早期柑橘黄龙病中的应用。使用一套近地高光谱成像系统采集了176枚柑橘叶片的高光谱图像作为实验样品,其中健康叶片60枚,黄龙病叶片60枚,缺锌叶片56枚。手工选取每幅叶片高光谱图像的病斑... 详细信息

讨论了基于高光谱成像技术光谱及纹理特征在识别早期柑橘黄龙病中的应用。使用一套近地高光谱成像系统采集了176枚柑橘叶片的高光谱图像作为实验样品,其中健康叶片60枚,黄龙病叶片60枚,缺锌叶片56枚。手工选取每幅叶片高光谱图像的病斑位置作为样品感兴趣区域（regions of interest,ROI）,计算其平均光谱反射率,并以此作为样品的反射光谱,光谱范围为396～1 010nm。样品光谱分别经过主成分分析（PXA）及连续投影算法（SPA）进行数据降维,再结合最小二乘支持向量机（LS-SVM）分类器建立分类模型。相比原始光谱,由PCA选取的前四个主成分及SPA选取的一组最佳波长组合（630.4,679.4,749.4和899.9 nm）建立的模型拥有更好的分类识别能力,其对三类柑橘叶片平均预测准确率分别为89.7%和87.4%。同时,从被选四个波长的每幅灰度图像中提取6个灰度直方图的纹理特征以及9个灰度共生矩阵的纹理特征再次构建分类模型。经SPA优选的10个纹理特征值进一步提高了分类效果,对三类柑橘叶片的识别正确率达到了100%,93.3%和92.9%。实验结果表明,同时包含光谱信息及空间纹理信息的高光谱图像在柑橘黄龙病的识别中显示了很大的潜力。

关键词：柑橘黄龙病高光谱成像分类纹理特征连续投影算法

MR-seq:measuring a single cell's transcriptome repeatedly by RNA-seq

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Science Bulletin 2017年第6期62卷 391-398页

作者： Lu Yang Zhaochun Ma Chen Cao Yuhao Zhang Xinglong Wu Raymond lee Boqiang Hu Lu Wen hao Ge Yanyi Huang Kaiqin Lao Fuchou Tang Biodynamic Optical Imaging Center School of Life Sciences Peking University Beijing 100871 China Thermo Fisher Scientific.South San Francisco CA 94080 USA Peking-Tsinghua Center for Life Sdences Beijing 100084 China Beijing International Center for Mathematical Research (BICMR) Peking University Beijing 100871 China College of Engineering Peking University Beijing 100871 China China Center for Molecular and Translationai Medicine (CMTM) Beijing 100101 China Beijing Advanced Innovation Center for Cenomics (ICG) Peking University Beijing 100871 China

We described a novel single-cell RNA-seq technique called MR-seq （measure a single-cell transcriptome repeatedly）, which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single ceils by measuring each single cell twice. We demonstrated that MR-seq gave sensitivity and reproducibility similar to the standard single-cell RNA-seq and increased the positive predicate value, Application of MR-seq to early mouse embryos identified hundreds of candidate intra-embryonic heterogeneous genes among mouse 2-, 4- and 8-cell stage embryos. MR-seq should be useful for detecting differentially exnre^ed ~enes ~rnnn~ ~ ~m^ll nHmhpr nf c^ll~

关键词： Single-cell RNA-seqDifferentially expressed geneslntra-embryonic heterogeneity Mouse early preimplantation embryos

Coexistence of hepatoma with mantle cell lymphoma in a hepatitis B carrier

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World Journal of Gastroenterology 2015年第45期21卷 12981-12986页

作者： Mu-Hsien lee Yu-Ching Lin hao-Tsai Cheng Wen-Yu Chuang Hsin-chih Huang Hsiao-Wen Kao Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital and Chang Gung University College of Medicine Department of Intervention and Imaging Chang Gung Memorial Hospital and Chang Gung University College of Medicine Department of Pathology Chang Gung Memorial Hospital and Chang Gung University College of Medicine Department of Hematology-Oncology Chang Gung Memorial Hospital and Chang Gung University College of Medicine

The coexistence of hepatocellular carcinoma(HCC) and non-Hodgkin's lymphoma(NHL) in the liver is rare. Reports show that these patients have cirrhotic livers or hepatitis virus infections before they develop HCC and NHL. We present a patient with hepatitis B virus infection who was transferred to our hospital with a newly detected liver mass; abdominal computed tomography examination showed one hypodense mass of 7 cm in diameter and multiple mesenteric and mediastinal lymph nodes. A liver tumor biopsy showed a hepatoma, and the pathologic findings from an inguinal lymph node excision showed mantle cell lymphoma. An immunohistochemical stain confirmed that the atypical lymphoid cells within the HCC were positive for the CD20, CD5 and cyclin D1 antigens. Taking these findings into account, the hepatic tumor was determined to be a HCC infiltrated by mantle cell lymphoma.

关键词： Hepatocellular carcinoma Mantle cell lymphoma Hepa

Integration of Promoter and Exon Arrays for colorectal carcinoma cell line shows pathways of neurodegenerative diseases are over-represented under Oxaliplatin treatment

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Integration of Promoter and Exon Arrays for colorectal carci...

Short-term developmental outcomes in neonates born to mothers with COVID-19 from Wuhan, China

第四届全国生物信息学与系统生物学学术大会

作者： Yi-An Ko Feng-Hsiang Chung chih-hao Chen Hsing-Chung lee Akon Higuchi 李宏谦 Graduate Institute of Systems Biology and Bioinformatics National Central University Cathay Medical Research Institute CathayGeneral HospitaI Department of Chemical Engineering and Material National CentralUniversity National Center for Theoretical Science

A novel method,a genome-wide unrestrained functional elements assay (UFEA),is employed to identify changes in regulatory elements and to pursue a comprehensive characterization of pathway enrichment of HCT-116 colorectal cancer cell line under Oxaliplatin *** from high-resolution promoter tiling arrays and exon arrays are analyzed in an integrated

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World Journal of Pediatrics 2021年第3期17卷 253-262页

作者： Ling-Kong Zeng Hua-Ping Zhu Tian-Tian Xiao Si-Cong Peng Wen-hao Yuan Jian-Bo Shao Lai-Shuan Wang Fei-Fan Xiao Shi-Wen Xia Shoo K.lee Kai Yan Wen-hao Zhou Department of Neonatology Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Neonatology Maternal and Child Health Hospital of Hubei ProvinceWuhanChina National Children’s Medical Center Children’s Hospital of Fudan UniversityShanghaiChina Department of Neonatology Chengdu Women's and Children's Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduChina Department of Neonatology Children's Hospital of Fudan UniversityNational Children's Medical CenterShanghaiChina Maternal-Infant Care Research Center(MiCARE)and Department of Pediatrics Mount Sinai HospitalTorontoONCanada Department of Pediatrics Department of Obstetrics and Gynecology and Dal la Lana School of Public HealthUniversity of TorontoTorontoONCanada

Background Coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is an emerging *** consequences of SARS-CoV-2 exposure in infants remain ***,this study aims to investigate whether neonates born to mothers with COVID-19 have adverse brain *** This multicenter observational study was conducted at two designated maternal and children's hospitals in Hubei Province,China's Mainland from February 1,2020 to May 15,*** born to mothers with COVID-19 were *** magnetic resonance imaging(MRI)findings,and volumes of grey and white matters,and physical growth parameters were observed at 44 weeks corrected gestational *** Of 72 neonates born to mothers with COVID-19,8(11%)were diagnosed with COVID-19,8(11%)were critically ill,and no deaths were *** the eight neonates that underwent brain MRI at corrected gestational age of 44 weeks,five neonates were diagnosed with *** these five neonates,three presented abnormal MRI findings including abnormal signal in white matter and delayed myelination in newborn 2,delayed myelination and brain dysplasia in newborn 3,and abnormal signal in the bilateral periventricular in newborn *** other three neonates without COVID-19 presented no significantly changes of brain MRI findings and the volumes of grey matter and white matter compared to those of healthy newborns at the equivalent age(P>0.05).Physical growth parameters for weight,length,and head circumference at gestational age of 44 weeks were all above the 3rd percentile for all *** Some of the neonates born to mothers with COVID-19 had abnormal brain MRI findings but these neonates did not appear to have poor physical *** findings may provide the information on the follow-up schedule on the neonates exposed to SARS-CoV-2,but further study is required to evaluate the association between the abnormal MRI findings and the exposure

关键词： Coronavirus disease 2019 Follow-up Neonate Severe acute respiratory syndrome coronavirus 2

A humidity resistant and high performance triboelectric nanogenerator enabled by vortex-induced vibration for scavenging wind energy

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Nano Research 2022年第4期15卷 3246-3253页

作者： Yan Wang Tianyu Chen Shuowen Sun Xiangyu Liu Zhiyuan Hu Zhenhui Lian Long Liu Qiongfeng Shi hao Wang Jianchun Mi Tongming Zhou Chengkuo lee Minyi Xu Dalian Key Lab of Marine Micro/Nano Energy and Self-powered System Marine Engineering CollegeDalian Maritime UniversityDalian 116026China Center for Intelligent Sensors and MEMS(CISM) National University of Singapore Engineering Drive 3 Singapore 117576Singapore3 College of EngineeringPeking UniversityBeijing 100871China School of Civil Environmental and Mining EngineeringThe University of Western AustraliaWA 6009Australia

Wind energy is a promising renewable energy source for a low-carbon *** study is to develop a fully packaged vortexinduced vibration triboelectric nanogenerator(VIV-TENG)for scavenging wind *** VIV-TENG consists of a wind vane,internal power generation unit,an external frame,four springs,a square cylinder and a circular *** internal power generation unit consists of polytetrafluoroethylene(PTFE)balls,a honeycomb frame and two copper *** from most of the previous wind energy harvesting TENGs,the bouncing PTFE balls are fully packaged in the square *** distinct design separates the process of contact electrification from the external environment,and at the same time avoids the frictional wear of the ordinary wind energy harvesting *** corresponding VIV parameters are investigated to evaluate their influence on the vibration behaviors and the energy *** state of the VIV-TENG corresponds to the high output performance from the *** distinct,robust structure ensures the full-packaged VIV-TENG can harvest wind energy from arbitrary directions and even in undesirable weather *** study proposes a novel TENG configuration for harvesting wind energy and the VIV-TENG proves promising powering micro-electro-mechanical appliances.

关键词： vortex-induced vibration wind energy triboelectric nanogenerator self-powered

不同风场结构下贺兰山地形降水的理想数值试验

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干旱区研究 2024年第8期41卷 1272-1287页

作者：李超隆霄曹怡清韩子霏王号郑景元兰州大学大气科学学院甘肃兰州730000 河北省气象灾害防御和环境气象中心河北石家庄050021 河北省气候中心河北石家庄050021

地形降水是我国西北地区最主要的降水类型之一,加深其形成机理的认识对提高预报能力有重要意义。本文以贺兰山东麓地区20次暴雨过程中的高空环境参数为基础构建不同类型风场垂直分布结构,利用WRF模式中的em_hill2d_x模块开展不同类型风... 详细信息

地形降水是我国西北地区最主要的降水类型之一,加深其形成机理的认识对提高预报能力有重要意义。本文以贺兰山东麓地区20次暴雨过程中的高空环境参数为基础构建不同类型风场垂直分布结构,利用WRF模式中的em_hill2d_x模块开展不同类型风场对降水模态影响的理想数值试验。结果表明:(1)有/无风垂直切变的两种不同类型干气流过山后其迎风坡均表现为地形对迎风坡气流的抬升作用,而背风坡波动表现出明显不同的变化特征,单层均一气流条件下背风坡主要表现为沿垂直方向传播的过山波,低空存在风切变的风场条件下背风侧主要体现背风波的特征,重力波呈水平与垂直传播共存的特征,且随着低空风切变增强水平传播的背风波特征越来越明显。(2)单层均一湿气流条件下模拟的降水主要位于迎风侧,背风侧在较强的下坡风影响下,降水强度相对较弱。当风速增大至10m·s^(-1)以上时,迎风侧云水含量大值区向山顶汇集,降水强度显著增强。低空存在风切变湿气流过山试验结果显示在迎风侧与背风侧均存在较强的降水中心,背风坡存在一深对流系统,随着风速增大,两侧降水均增强。(3)在低空东风高空西风的条件下,背风侧降水显著减弱,降水更加集中于上游山顶附近,强度也有一定的增强,这与高空西风的出现使迎风坡上升气流加强,且不利于水汽向下游的输送有关,这是贺兰山脉两侧降水特征存在显著差异的主要原因之一。

关键词：地形降水理想试验重力波对流系统贺兰山

Development of Combined Trochoidal Profile of a Gerotor Pump

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Journal of Applied Mathematics and Physics 2016年第1期4卷 28-32页

作者： hao Liu Jae-Cheon lee Department of Mechanical and Automotive Engineering Keimyung University Daegu South Korea

Gerotor pumps are a kind of internal gear pump, widely used in applications of lubricating system of on-road or off-road engines. Normally their outer rotors are the circular arc profile while their inner rotor profile is the conjugate curve of the outer rotor. This research focuses on how to derive the combined trochoidal profile of a gerotor pump. The hypocycloidal and epicycloidal profile of the inner rotor is generated at first. And then the profile of the outer rotor, based on the inner rotor profile, is obtained by analytical approach and computationally graphical approach. The paper provides feasibility to develop the unconventional profile of a gerotor pump.

关键词： Hypocycloidal and Epicycloidal Profile Conjugate Profile Computationally Graphical Approach Gerotor Pump