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Determination of particulate polycyclic aromatic hydrocarbons in ambient air by gas chromatography-mass spectrometry after molecularly imprinted polymer extraction

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Journal of Environmental Sciences 2023年第2期124卷 644-654页

作者： Maria chiara Pietrogrande Giorgia Demaria Mara Russo Department of Chemical Pharmaceutical and Agraricultural SciencesUniversity of FerraraVia Fossato di Mortara 17/1944121 FerraraItaly

A solid phase extraction procedure(SPE)is described for the quantitative analysis of polycyclic aromatic hydrocarbons(PAHs)in atmospheric particulate matter(PM),as ubiquitous environmental pollutants routinely measured in air qualitymonitoring.A SPE cartridge was used based on a molecular imprinted polymer(MIP-SPE)properly tailored for selective retention of PAHs with 4 and more benzene fused *** performance of the clean-up procedure was evaluated with the specific concern of selective purification towards saturated hydrocarbons,which are the PM components mostly interfering GC analysis of target *** optimized operative conditions,the MIP-SPE provided analyte recovery close to 95%for heavier PAHs,from benzo(α)pyrene to benzo(ghi)perylene,and close to 90%for four benzene rings PAHs,with good reproducibility(RSDs:2.5%-5.9%).Otherwise,C_(17)-C_(32) n-alkanes were nearly completely *** proposed method was critically compared with Solid Phase Micro Extraction(SPME)using a polyacrylate *** methods were successfully applied to the analysis of ambient PM2.5 samples collected at an urban polluted *** the two procedures,the MIP-SPE provided the highest recovery(R%≥93%)for PAHs with 5 and more benzene rings,but lower for lighter *** contrast,SPME showed a mean acceptable R%value(∼80%)for all the investigated PAHs,except for the heaviest PAHs in the most polluted samples(R%:110%-138%),suggesting an incomplete purification from the interfering n-hydrocarbons.

关键词： Polycyclic aromatic hydrocarbons Atmospheric particle matter Molecularly imprinted polymers-solid-phase extraction Solid phase micro extraction GC/MS analysis

Playing together,laughing together:rapid facial mimicry and social sensitivity in lowland gorillas

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Current Zoology 2022年第5期68卷 560-569页

作者： chiara Bresciani Giada Cordoni Elisabetta Palagi Natural History Museum University of PisaVia Roma 79Calci(Pisa)56011Italia Department of Biology Unit of EthologyUniversity of PisaVia A.Volta 6Pisa 56126Italia

n nonhuman animals,the phenomenon of rapid facial mimicry(RFM)—the automatic,involuntary,and rapid(<1 s)replication of others’facial expressions—has been mainly investigated in the playful *** immature lowland gorillas Gorilla gorilla gorilla both play face(PF)and full PF(FPF)are rapidly mimicked between the *** makes the species suitable to test hypotheses on the factors influencing RFM during *** observations on 3 captive groups of lowland gorillas(N=27)revealed that contrary to expectations,the closeness of social bond negatively influenced the occurrence of RFM but it did not affect either RFM latency or its overlapping index(OVERLAP).RFM was affected by the degree of symmetry of play fighting:the more balanced the session,the higher the occurrence of *** of the same sex class responded faster than players of different *** findings suggest that RFM may help synchronizing behaviors of playmates matching in size(same-sex)and promote symmetric playful interactions.“Laughing together”(measured by the RFM OVERLAP)lasted longer when the responder perfectly mirrored the partner expression(PF→PF;FPF→FPF).If PF and FPF convey information on the different play roughness degree,through“laughing together”the players could coordinate their actions and share positive moods and playful *** the perfect congruency in the motor resonance,also known as social sensitivity,can foster a possible emotional dialogue between gorillas remains to be investigated.

关键词： emotional resonance facial overlapping Gorilla gorilla gorilla matching playmates mirror response play communication

Circulating tumor cells isolation:the "post-EpCAM era"

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Chinese Journal of Cancer Research 2015年第5期27卷 461-470页

作者： Cristina Raimondi chiara Nicolazzo Angela Gradilone Dipartimento Medicina Molecolare Sapienza Universita di RomaRoma Italy

Circulating tumor cells （CTCs） represent a submicroscopic fraction detached from a primary tumor and in transit to a secondary site. The prognostic significance of CTCs in metastatic cancer patients was demonstrated for the first time more than ten years ago. To date, it seems clear enough that CTCs are highly heterogeneous and dynamically change their shape. Thus, the inadequacy of epithelial cell adhesion molecule （EpCAM） as universal marker for CTCs detection seems unquestionable and alternative methods able to recognize a broader spectrum of phenotypes are definitely needed. In this review the pleiotropic functions of EpCAM are discussed in detail and the role of the molecule in the biology of CTCs is critically dissected.

关键词： Epithelial cell adhesion molecule(EpCAM) circulating tumor cells(CTCs) epithelial-mesenchymal transition EpICD

Hearing(Technological)Anomalies:An Analysis of Han Song's"The Rebirth Bricks"

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2020年第2期14卷 228-253页

作者： chiara Cigarini Modern and Contemporary Chinese Literature Beijing Normal UniversityBeijing 100875China

Contemporary Chinese science fiction author and journalist Han Song's works often cross the lines dividing reality from imagination,science fiction from literary mainstream,technology from the *** article,focusing in particular on Han's novella"The Rebirth Bricks"(Zaisheng zhuan),aims to investigate the role played by the senses in a shift from the science fictional novum to the fictional"uncanny."Featuring technological bricks,haunted by sounds of the dead which are perceived through sight and hearing,this novella is analyzed from the standpoint of this perceptual complementarity which expresses Han Song's"science fictional re-enchantment,"a re-use of supernatural themes of the past that allow him to express the(technological)anomalies of China's current reality.

关键词： Chinese science fiction Han Song science fictional re-enchantment zhiguai sensory

Induced pluripotent stem cells for defining Parkinsonian patient subtypes:a further step toward precision medicine

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Neural Regeneration Research 2022年第4期17卷 767-769页

作者： Federica Bono Cristina Missale chiara Fiorentini Division of Pharmacology Department of Molecular and Translational MedicineUniversity of BresciaBresciaItaly

Introduction:Parkinson's disease PD is a common neurodegenerative disorder mainly characterized by the progressive decline of motor function with a prevalence that is greatly increasing since the pathology is principally driven by *** currently available treatments only mitigate motor symptoms;therefore,a great deal of studies are aimed at finding novel drugs that are able to arrest the evolution of the *** progressive death of nigrostriatal dopaminergic(DA)neurons and the formation of the typical Lewy bodies neuronal inclusions containing misfolded alpha-synuclein are two key histopathological features of the ***,it is now evident that PD has to be considered as a heterogeneous disorder that includes subtypes with distinctive clinical and biological characteristics(Ryden and Lewis,2019).

关键词： drugs progressive finding

New Role for an Old Rule: N-end Rule-Mediated Degradation of Ethylene Responsive Factor Proteins Governs Low Oxygen Response in Plants

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Journal of Integrative Plant Biology 2013年第1期55卷 31-39页

作者： Francesco Licausi chiara Pucciariello Pierdomenico Perata Plant Lab Institute of Life Sciences Scuola Superiore Sant' Anna

The N-end rule pathway regulates protein degradation, which depends on exposed N-terminal sequences in prokaryotes and eukaryotes. In plants, conserved and specific enzymes stimulate selective proteolysis. Although a number of developmental and growth phenotypes have been reported for mutants in the N-end rule, its function has remained unrelated to specific physiological pathways. The first report of the direct involvement of the N-end rule in stress responses focused on hypoxic signaling and how the oxygen-dependent oxidation of cystein promotes the N-end rule-mediated degradation of ethylene responsive factor （ERF）-VII proteins, the master regulators of anaerobic responses. It has been suggested that plants have evolved specific mechanisms to tune ERF-VII availability in the nucleus. In this review, we speculate that ERF-VII proteins are reversibly protected from degradation via membrane sequestration. The oxidative response in plants subjected to anoxic conditions suggests that reactive oxygen and nitrogen species （reactive oxygen species and reactive nitrogen species） may interact or interfere with the N-end rule pathway-mediated response to hypoxia.

关键词： Ethylene response factor flooding hypoxia N-end rule pathway ubiquitin.

Fungal spores and pollen in particulate matter collected during agricultural activities in the Po Valley(Italy)

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Journal of Environmental Sciences 2016年第8期28卷 229-240页

作者： chiara Telloli Milvia Chicca Marilena Leis Carmela Vaccaro Italian National Agency for New Technologies Energy and Sustainable Economic Development (ENEA) Technical Unit for Environmental Assessment Models Methods and Technologies (UTVALAMB) Air Quality Laboratory (AIR) Department of Life Science and Biotechnologies Ferrara University Department of Physics and Earth Sciences Ferrara University

Airborne particulate matter（PM） containing fungal spores and pollen grains was sampled within a monitoring campaign of wheat threshing, plowing and sowing agricultural operations. Fungal spores and pollen grains were detected and identified on morphological basis. No studies were previously available about fungal spore and pollen content in agricultural PM in the Po Valley. Sampling was conducted in a Po Valley farmland in Mezzano（Ferrara, Italy）. The organic particles collected were examined by scanning electron microscopy with energy dispersive X-ray spectrometer. Fungal spores and pollen grains were identified when possible at the level of species. The most frequent components of the organic particles sampled were spores of Aspergillus sp., which could represent a risk of developing allergies and aspergillosis for crop farmers.

关键词： Wheat Aspergillosis Emilia-Romagna Morphology SEM–EDS

Exploring pre-degenerative alterations in humans using induced pluripotent stem cell-derived dopaminergic neurons

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Neural Regeneration Research 2017年第7期12卷 1068-1070页

作者： Federica Bono chiara Fiorentini Division of Pharmacology Department of Molecular andTranslational Medicine University of Brescia Brescia Italy

Understanding the cellular and molecular mechanisms underlying human neurological disorders is hindered by both the complexity of the disorders and the lack of suitable experimental models recapitulating key pathological features of the *** is a crucial issue since a limited understanding of pathogenic mechanisms precludes the development of drugs counteracting the progression of the *** neurological disorders,

关键词： in Exploring pre-degenerative alterations in humans using induced pluripotent stem cell-derived dopaminergic neurons stem DAT been that of cell PSC

A new three-dimensional finite-volume non-hydrostatic shock-capturing model for free surface flow

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Journal of Hydrodynamics 2017年第4期29卷 552-566页

作者： Francesco Gallerano Giovanni Cannata Francesco Lasaponara chiara Petrelli Department of Civil Constructional and Environmental Engineering Sapienza University of Rome

In this paper a new finite-volume non-hydrostatic and shock-capturing three-dimensional model for the simulation of wave-structure interaction and hydrodynamic phenomena（wave refraction, diffraction, shoaling and breaking） is proposed. The model is based on an integral formulation of the Navier-Stokes equations which are solved on a time dependent coordinate system： a coordinate transformation maps the varying coordinates in the physical domain to a uniform transformed space. The equations of motion are discretized by means of a finite-volume shock-capturing numerical procedure based on high order WENO reconstructions. The solution procedure for the equations of motion uses a third order accurate Runge-Kutta（SSPRK） fractional-step method and applies a pressure corrector formulation in order to obtain a divergence-free velocity field at each stage. The proposed model is validated against several benchmark test cases.

关键词： Three-dimensional simulation non-hydrostatic wave-structure interaction flow-structure interaction shock-capturing