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Phyton-International Journal of Experimental Botany 2024年第3期93卷 627-640页

作者： Haoxue Wu Xiaohan Zhang Xiaoyu Chen Kang Li Aixia Xu Zhen Huang Jungang Dong chengyu yu College of Agronomy Northwest A&F UniversityYangling712100China

Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus *** is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases FAD2 and FAD3,which con-trol the last two steps of ALA biosynthesis during seed oil *** this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype *** expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and *** three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnFAD3.C4 and *** A28 and SW,substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5,an absence of BnFAD2.C1 locus,and a 549 bp long deletion on the BnFAD3.A3 promoter were *** proﬁle of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous *** mutations in BnFAD3 in the high-ALA accessions are reported for theﬁrst *** identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes.

关键词： Brassica napus linolenic acid FAD2 FAD3 promoter coding sequences mutation

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中国传统义利观的时代价值判析

文化创新比较研究 2024年第11期8卷 59-62页

作者：余成煜桂林航天工业学院图书馆广西桂林541004

当前我国正处于社会转型时期,需要传承和弘扬中华优秀传统文化、坚定文化自信。中国传统义利观中的以义为先、以义谋利、义中取利、义利合一等论述,对于我国当今社会发展有着重要的现实价值,主要体现在:坚守以义为先,避免人际交往的失... 详细信息

当前我国正处于社会转型时期,需要传承和弘扬中华优秀传统文化、坚定文化自信。中国传统义利观中的以义为先、以义谋利、义中取利、义利合一等论述,对于我国当今社会发展有着重要的现实价值,主要体现在:坚守以义为先,避免人际交往的失信化、利己化和功利化,是构建和谐人际关系的基础;信守义中谋利,既是企业和个人发展的前提,也有利于个人道德素质的提升和企业软实力的增强,推进社会的持续发展;遵循义中取利,能帮助人们树立合理的财富观;坚持义利合一,公利即为义,也就是在个人利益与集体利益发生矛盾时,以集体利益为首,促进健康群己观的形成。

关键词：传统文化儒家伦理义利观现实价值辨析

Human RBCs blood group conversion from A to O using a novel α-N-acetylgalactosaminidase of high specific activity

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Chinese Science Bulletin 2008年第13期53卷 2008-2016页

作者： yu chengyu XU Hua WANG LiSheng ZHANG JianGeng ZHANG YangPei Department of Blood Molecular Biology Institute of Military Transfusion Academy of Military Medical Science Beijing 100850 China Shaanxi Blood Centre Xi'an 710061 China Department of Experimental Hematology Institute of Radiation Medicine Academy of Military Medical Science Beijing 100850 China

α-N-acetylgalactosaminidase (αNAGA) can convert group A human red blood cells (RBCs) to group O. One novel αNAGA gene was cloned by PCR from Elizabethkingia meningosepticum isolated from a domestic clinical sample. Pure recombinant αNAGA was obtained by genetic engineering and protein purification with a calculated molecule of 49.6 kD. αNAGA was selective for terminal α-N-acetylgalacto-samine residue with a high specific activity. αNAGA could completely remove A antigens of 1 U (about 100 mL) group A1 or A2 RBCs in 1 h at pH 6.8 and 25℃ with a consumption of 1.5 or 0.4 mg recombinant enzyme. Enzyme-converted group A RBCs did not agglutinate after being mixed with monoclonal anti-A or sera of groups A,B,AB and O. Other blood group antigens except ABO had no change. FCM analy-sis showed that A antigens and A1 antigens disappeared while H antigens increased. It indicated that αNAGA successfully converted human blood group A RBCs to universally transfusable group O RBCs without the risk of ABO-incompatible transfusion reactions. This αNAGA was suitable for producing universal RBCs to increase clinical transfusion safety,improve the RBCs supply,and to decrease transfusion cost and support transfusion service in case of emergency.

关键词：血型转换输血反应 A抗原免疫反应

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芥菜bZIP基因家族的鉴定和表达分析

分子植物育种 2024年第7期22卷 2104-2118页

作者：代春艳张笑晗王晓丽弓琼苏醒成耀华张明政于澄宇西北农林科技大学农学院杨凌712100

碱性亮氨酸拉链(bZIP)是一类广泛存在真核生物中的转录因子家族,参与多种植物生长发育及生物和非生物胁迫的响应过程,对下游许多基因的表达具有强烈调控作用。为了解芥菜bZIP基因家族的基因结构,成员之间的相互关系以及在非生物胁迫下... 详细信息

碱性亮氨酸拉链(bZIP)是一类广泛存在真核生物中的转录因子家族,参与多种植物生长发育及生物和非生物胁迫的响应过程,对下游许多基因的表达具有强烈调控作用。为了解芥菜bZIP基因家族的基因结构,成员之间的相互关系以及在非生物胁迫下的反应机制。本研究利用生物信息学方法,从Brassica Database (BRAD)数据库下载的芥菜基因组中鉴定到81个BjubZIP基因,其中34.57%的基因缺乏内含子,59.26%的BjubZIP等电点小于7,表明芥菜中有超过一半的bZIP蛋白为酸性蛋白,亚细胞定位预测所有的基因均位于细胞核。将81个BjubZIP进一步分为与拟南芥基因相对应的6个亚群,同一亚群的motif数量和基因结构高度相似。利用公共数据库中的芥菜转录组数据,对BjubZIP基因家族在干旱和高温胁迫下的表达分析,发现A亚群基因中的BjubZIP02、BjubZIP21、BjubZIP28、BjubZIP38、BjubZIP50、Bjub ZIP62、BjubZIP59在高温和干旱胁迫下均处于上调状态,推测芥菜中的这7个基因可能与拟南芥A亚群基因的功能相似,参与了在非生物胁迫下相应的生理过程。本研究结果可为今后芥菜bZIP家族成员的基因克隆和在胁迫反应中的功能研究提供参考。

关键词：芥菜(Brassica juncea L.) bZIP 基因家族表达分析

Catch organism assemblages along artificial reefs area and adjacent waters in Haizhou Bay

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Acta Oceanologica Sinica 2024年第2期43卷 34-42页

作者： Shike Gao Bin Xie chengyu Huang Xiao Zhang Shuo Zhang Wenwen yu College of Marine Living Resource Sciences and Management Shanghai Ocean UniversityShanghai 201306China Jiangsu Research Institute of Marine Fisheries Nantong 226007China Third Institute of Oceanography Ministry of Natural ResourcesXiamen361005China Joint Laboratory for Monitoring and Conservation of Aquatic Living Resources In the Yangtze Estuary Shanghai 200000China

To better understand the community patterns mediated by connectivity in artificial reefs of coastal areas, it is necessary to understand the distribution and coexistence of organisms with artificial reefs area and adjacent waters. This study was conducted to examine main catches assemblages collected by trawls in Haizhou Bay,which included five habitats: the artificial reef area(AR), aquaculture area(AA), natural area(NA), estuary area(EA) and comprehensive effect area(CEA). The result shows that the total abundances of species in the five habitats were highly different(univariate PERMANOVA: P = 0.001, n = 24), but some species were also unique in their habitat(e.g. Scapharca subcrenata and Glossaulax didyma in AA). The body size distribution of specific species between habitats are different. For Collichthys lucidus, their body size in AR(14.63 cm ± 1.64 cm) and EA(14.3 cm ± 0.85 cm) is higher than that in NA(10.65 cm ± 1.64 cm), CEA(11.28 cm ± 1.85 cm) and AA(12.1 cm ±0.43 cm), which indicates the potential connection from AR to EA mediated by their adult population. We concluded that artificial reefs in AR can be considered key components that have the ability to support species assemblages in adjacent habitats. This study has implications for the conservation and monitoring of species assemblages in coastal areas in terms of that artificial reefs can be applied in different stages of habitat protection implementation and in different combinations of scenarios.

关键词： assemblage artificial reefs adjacent water Haizhou Bay

Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed(Brassica napus)

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Oil Crop Science 2021年第4期6卷 169-174页

作者： Xiaohan Zhang Jinglong Lian Chunyan Dai Xiaoli Wang Mingzheng Zhang Xing Su Yaohua Cheng chengyu yu College of Agronomy Northwest A&F UniversityTaicheng Road 3YanglingShaanxi712100China Institute of Agricultural Sciences Garze Prefecture.Lucheng South Road 88KangdingSichuan626000China

Alpha linolenic acid(ALA)is an essential polyunsaturated fatty acids that can improve human *** is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%.Two rapeseed germplasms YH25005 and R8Q10 with high content(up to 21%)ALA were developed by intervarietal *** were used as the maternal parent(P_(1))when crossed with a low ALA parent SW(P2)to produce the seeds of the F1 hybrid,F2,and backcrosses to P1(BCP1)and P2(BCP2).A multigeneration joint segregation analysis was conducted to determine major gene t polygene effects of the content of three major unsaturated fatty acids including oleic(OA),linoleic(LA)and *** results showed that,although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005,all the inheritances of OA,LA and ALA in YH25005×SW and R8Q10×SW followed a genetic model of‘two pairs of additive major genes t additive-dominant polygenes’.It is suggested that the contents of OA,LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate ***,total genetic effect of the polygenes was greater than that of the two major genes,especial on ALA *** indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA *** results are useful for high ALA rapeseed breeding and future work of gene mapping.

关键词： Rapeseed High linolenicacid Fattyacid Genetic analysis Major gene Polygene

Recent advances in visible light-mediated chemical transformations of enaminones

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Chinese Chemical Letters 2024年第2期35卷 4-15页

作者： yu Han Liyun Zhou chengyu Wang Shangti Feng Rong Ma Jie-Ping Wan College of Chemistry and Chemical Engineering Jiangxi Normal UniversityNanchang 330022China School of Chemistry and Chemical Engineering Linyi UniversityLinyi 276000China Linyi Sanfeng Chemical Co. Ltd.Linyi 276000China

Enaminones,which possesses both the nucleophilic enamine as well as electrophilic enone structures,are well known versatile building blocks in organic ***,visible light-mediated reactions have emerged as useful synthetic strategy with enhanced *** the last decade,various photochemical transformations of enaminones have been developed to construct cyclic or acyclic *** this review,we describe the recent advances in visible light-mediated chemical transformations of *** discussion on the reaction mechanism of the related reactions is given to provide guide to the ***,a summary on the existing challenges and the future outlook towards the development of practical photocatalytic reactions of enaminones is also presented.

关键词： Enaminone Visible light C-H functionalization C=C bond cleavage Cyclization Multicomponent reactions

Hydrogen-bonded organic framework modified separator for simultaneously enhancing the safety and electrochemical performance of Ni-rich lithium-ion battery

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Journal of Energy Chemistry 2024年第9期96卷 72-78页

作者： chengyu Han yu Cao Ming Yang yuhan Wang Di Tang Shaojie Zhang Yiran Jia Yiming Zhang Hern Kim Fusheng Pan Zhongyi Jiang Jie Sun Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Science and Technology on Power Sources Laboratory Tianjin Institute of Power SourcesTianjin 300384China Haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192China Quzhou Institute for Innovation in Resource Chemical Engineering Quzhou 324000ZhejiangChina Department of Energy Science and Technology Director Environmental Waste Recycle InstituteYongin 17058Republic of Korea School of Chemistry and Chemical Engineering Hainan UniversityHaikou 570228HainanChina

Nickel-rich layered oxide cathode(LiNi_(x)Co_(y)Mn_(1−x−y)O_(2),x>0.5,NCM)shows substantial potential for applications in longer-range electrical ***,the rapid capacity decay and serious safety concerns impede its practical *** work provides a hydrogen-bonded organic framework(HOF)modification strategy to simultaneously improve the electrochemical performance,thermal stability and incombustibility of *** cyanurate(MCA),as a low-cost and reliable flame-retardant HOF,was implemented in the separator modification layer,which can prevent the battery short circuit even at a high *** addition,the supermolecule properties of MCA provide unique physical and chemical microenvironment for regulating ion-transport behavior in *** MCA coating layer enabled the nickel-rich layered oxide cathode with a high-capacity retention of 90.3%after 300 cycles at 1.0 ***,the usage of MCA in lithium-ion batteries(LIBs)affords a simple,low-cost and efficient strategy to improve the security and service life of nickel-rich layered cathodes.

关键词： Hydrogen-bonded organic framework Modified separator Ni-richlayered oxide cathode Thermal runaway Li^(+)transference number

LncFASA promotes cancer ferroptosis via modulating PRDX1 phase separation

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Science China(Life Sciences) 2024年第3期67卷 488-503页

作者： Xiao Fan Fangzhou Liu Xiang Wang Ying Wang yu Chen chengyu Shi Xinwan Su Manman Tan Qingfeng Yan Jinrong Peng Jianzhong Shao Yan Xiong Aifu Lin MOE Laboratory of Biosystem Homeostasis and Protection College of Life SciencesZhejiang UniversityHangzhou 310058China Key Laboratoryof Cancer Prevention and Intervention China National Ministry of EducationHangzhou 310009China Cancer Center Zhejiang UniversityHangzhou 310058China Key Laboratory for Cell and Gene Engineering of Zhejiang Province Hangzhou 310058China Department of Central Laboratory the First People's Hospital of HuzhouHuzhou 313000China Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province Affiliated Hangzhou First People's HospitalZhejiang University School of MedicineHangzhou 310006China MOE Key Laboratory for Molecular Animal Nutrition College of Animal SciencesZhejiang UniversityHangzhou 310058China Department of Orthopedic Surgery the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310000China Breast Center of the First Affiliated Hospital School of MedicineZhejiang UniversityHangzhou 310003China International School of Medicine International Institutes of MedicineThe 4th Affiliated Hospital of Zhejiang University School of MedicineYiwu 322000China

Ferroptosis, a unique type of non-apoptotic cell death resulting from iron-dependent lipid peroxidation, has a potential physiological function in tumor suppression, but its underlying mechanisms have not been fully elucidated. Here, we report that the long non-coding RNA(lncRNA) LncFASA increases the susceptibility of triple-negative breast cancer(TNBC) to ferroptosis. As a tumor suppressor, LncFASA drives the formation of droplets containing peroxiredoxin1(PRDX1), a member of the peroxidase family, resulting in the accumulation of lipid peroxidation via the SLC7A11-GPX4 axis. Mechanistically, LncFASA directly binds to the Ahpc-TSA domain of PRDX1, inhibiting its peroxidase activity by driving liquid-liquid phase separation, which disrupts intracellular ROS homeostasis. Notably, high LncFASA expression indicates favorable overall survival in individuals with breast cancer, and LncFASA impairs the growth of breast xenograft tumors by modulating ferroptosis. Together, our findings illustrate the crucial role of this lncRNA in ferroptosis-mediated cancer development and provide new insights into therapeutic strategies for breast cancer.

关键词： long non-coding RNA peroxiredoxin liquid-liquid phase separation ferroptosis breast cancer

法治规范体系视域下党内法规保留的功能定位与规范建构

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河南社会科学 2024年第6期32卷 12-21页

作者：喻少如唐成余西南政法大学行政法学院/纪检监察学院重庆401120

党内法规保留是将法律保留相关原理引入党内法规制度建设中形成的有关党内事务制定权配置的制度。在中国特色社会主义法治体系的法治背景下,党内法规保留应以法治规范体系作为制度建构的基本视角。基于与法律保留的功能比较,党内法规保... 详细信息

党内法规保留是将法律保留相关原理引入党内法规制度建设中形成的有关党内事务制定权配置的制度。在中国特色社会主义法治体系的法治背景下,党内法规保留应以法治规范体系作为制度建构的基本视角。基于与法律保留的功能比较,党内法规保留具有统制、区分、整合、保障等功能。在法治规范体系视域下,当前党内法规保留制度存在明显结构缺陷。可以从重塑党内法规保留的结构理念、重新阐释《中国共产党党内法规制定条例》第四条第一款的保留对象、建立相对保留制度、单列党章保留事项、对中央党内法规绝对保留事项进行内涵解读等方面进行结构重塑与完善。此外,在党内法规保留的保留标准上,应确立面向从严治党的重要事项保留标准。

关键词：党内法规保留法治规范体系法律保留党内法规与国家法律的衔接与协调从严治党