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Genes & Diseases 2024年第3期11卷 55-58页

作者： chengyu li Deyuan Pan Wan lin Danxia Deng Yingxin Zhou Hui Zhao Feng Pan liyan Xu Enmin li lin Long The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area Department of Biochemistry and Molecular BiologyShantou University Medical CollegeShantouGuangdong 515041China Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology Institute of Oncologic PathologyShantou University Medical CollegeShantouGuangdong 515041China Institute of Basic Medical Science Cancer Research CenterShantou University Medical CollegeShantouGuangdong 515041China Clinical Research Center Shantou Central HospitalShantouGuangdong 515041China

Esophageal cancer is one of the leading causes of cancer death in the world,with approximately half of the new cases occurring in China every year.^(1)Esophageal squamous cell carcinoma(ESCC)is the main subtype,accounting for more than 90%,and the five-year survival rate is less than 10%.Using large-scale genome analysis,many driver mutations and key pathways associated with ESCC have been identified.However,these genomic signatures have not improved the clinical management of EscC patients,or established effective targeted therapy.^(2)Esophageal cancer still lacks representative molecular markers.

关键词： cancer esophageal Esophageal

High-Throughput Automatic Training System for Spatial Working Memory in Free-Moving Mice

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Neuroscience Bulletin 2019年第3期35卷 389-400页

作者： Shimin Zou chengyu tony li Institute of Neuroscience State Key Laboratory of NeuroscienceChinese Academy of SciencesCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Center for Brain Science and Brain-Inspired TechnologyShanghai 200031China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China

Effcient behavioral assays are crucial for understanding the neural mechanisms of cognitive functions.Here, we designed a high-throughput automatic training system for spatial cognition(HASS) for free-moving mice.Mice were trained to return to the home arm and remain there during a delay period. Software was designed to enable automatic training in all its phases, including habituation, shaping, and learning. Using this system, we trained mice to successfully perform a spatially delayed nonmatch to sample task, which tested spatial cognition,working memory, and decision making. Performance depended on the delay duration, which is a hallmark of working memory tasks. The HASS enabled a human operator to train more than six mice simultaneously with minimal intervention, therefore greatly enhancing experimental efficiency and minimizing stress to the mice.Combined with the optogenetic method and neurophysiological techniques, the HASS will be useful in deciphering the neural circuitry underlying spatial cognition.

关键词： Cognitive functions Automatic training Free-moving mice Working memory Spatial cognition

Instability mechanism of mining roadway passing through fault at different angles in kilometre-deep mine and control measures of roof cutting and NPR cables

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Journal of Mountain Science 2024年第1期21卷 236-251页

作者： SUN Xiaoming WANG Jian ZHAO Wenchao MING Jiang ZHANG Yong li Zhihu MIAO chengyu GUO Zhibiao HE Manchao State Key Laboratory for Geomechanics and Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China School of Mechanics and Civil Engineering China University of Mining and Technology(Beijing)Beijing 100083China

The angle α between the fault strike and the axial direction of the roadway produces different damage characteristics. In this paper, the research methodology includes theoretical analyses, numerical simulations and field experiments in the context of the Daqiang coal mine located in Shenyang, China. The stability control countermeasure of "pre-splitting cutting roof + NPR anchor cable"(PSCR-NPR) is simultaneously proposed. According to the different deformation characteristics of the roadway, the faults are innovatively classified into three types, with α of type I being 0°-30°, α of type II being 30°-60°, and α of type III being 60°-90°. The full-cycle stress evolution paths during mining roadway traverses across different types of faults are investigated by numerical simulation. Different pinch angles α lead to high stress concentration areas at different locations in the surrounding rock. The non-uniform stress field formed in the shallow surrounding rock is an important reason for the instability of the roadway. The pre-cracked cut top shifted the high stress region to the deep rock mass and formed a low stress region in the shallow rock mass. The high prestressing NPR anchor cable transforms the non-uniform stress field of the shallow surrounding rock into a uniform stress field. PSCR-NPR is applied in the fault-through roadway of Daqiang mine. The low stress area of the surrounding rock was enlarged by 3-7 times, and the cumulative convergence was reduced by 45%-50%. It provides a reference for the stability control of the deep fault-through mining roadway.

关键词： Kilometre-deep mine Fault Mining roadway Failure mechanism Pre-splitting cutting roof High pre-stress NPR anchor cable

Elevated peripheral levels of receptor-interacting protein kinase 1(RIPK1)and IL-8 as biomarkers of human amyotrophic lateral sclerosis

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Signal Transduction and Targeted Therapy 2024年第1期9卷 271-278页

作者： Jun Wei Min li Zhi Ye Xinqian Hu Xiaoyan He Jia Wang Gaofeng Chen chengyu Zou Daichao Xu Hongbing Zhang Junying Yuan Yunhong Zha Institute of Neural Regeneration and Repair and Department of Neurology The First College of Clinical Medical ScienceYichang Central HospitalCollege of Basic Medical ScienceChina Three Gorges UniversityHubei Province Clinical Medical Research Center for Rare Diseases of Nervous SystemYichang 443000China Interdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai Key Laboratory of Aging StudiesShanghai 201210China State Key Laboratory of Common Mechanism Research for Major Diseases Haihe Laboratory of Cell EcosystemDepartment of PhysiologyInstitute of Basic Medical Sciences and School of Basic MedicineChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Amyotrophic lateral sclerosis(ALS)is a devastating fatal neurodegenerative disease with no cure.Receptor-interacting protein kinase 1(RIPK1)has been proposed to mediate pathogenesis of ALS.Primidone has been identified as an old drug that can also inhibit RIPK1 kinase.We conducted a drug-repurposing biomarker study of primidone as a RIPK1 inhibitor using SOD1G93A mice and ALS patients.SOD1G93A mice treated with primidone showed significant delay of symptomatic onset and improved motor performance.One-hundred-sixty-two ALS participants dosed daily with primidone(62.5 mg)completed 24-week follow-up.A significant reduction was showed in serum levels of RIPK1 and IL-8,which were significantly higher in ALS patients than that of healthy controls(Plinical biomarkers for the activation of RIPK1 in central nervous system in human ALS patients.Repurposing primidone may provide a promising therapeutic strategy for ALS.The effect of primidone for the treatment of other inflammatory diseases may also be considered,since the activation of RIPK1 has been implicated in mediating a variety of inflammatory diseases including COVID-19-associated cytokine release syndrome(CRS).(ChiCTR2200060149).

关键词： RIPK1 diseases Serum

Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed(Brassica napus L.)Germplasm with High/Low linolenic Acid Trait

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 627-640页

作者： Haoxue Wu Xiaohan Zhang Xiaoyu Chen Kang li Aixia Xu Zhen Huang Jungang Dong chengyu Yu College of Agronomy Northwest A&F UniversityYangling712100China

Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus breeding.B.napus is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases FAD2 and FAD3,which con-trol the last two steps of ALA biosynthesis during seed oil accumulation.In this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype ZS11.The expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW.The three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnFAD3.C4 and BnFAD3.A3.In A28 and SW,substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5,an absence of BnFAD2.C1 locus,and a 549 bp long deletion on the BnFAD3.A3 promoter were detected.The proﬁle of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies.The mutations in BnFAD3 in the high-ALA accessions are reported for theﬁrst time.In identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes.

关键词： Brassica napus linolenic acid FAD2 FAD3 promoter coding sequences mutation

Evolutionary divergence of subgenomes in common carp provides insights into speciation and allopolyploid success

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Fundamental Research 2024年第3期4卷 589-602页

作者： lin Chen chengyu li Bijun li Xiaofan Zhou Yulin Bai Xiaoqing Zou Zhixiong Zhou Qian He Baohua Chen Mei Wang Yaguo Xue Zhou Jiang Jianxin Feng Tao Zhou Zhanjiang liu Peng Xu State Key Laboratory of Mariculture Breeding College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China Integrative Microbiology Research Centre South China Agricultural UniversityGuangzhou 510642China College of Fisheries Henan Normal UniversityXinxiang 453007China Henan Academy of Fishery Science Zhengzhou 450044China Department of Biology College of Arts and SciencesSyracuse UniversitySyracuse 13244USA

Hybridization and polyploidization have made great contributions to speciation,heterosis,and agricultural production within plants,but there is still limited understanding and utilization in animals.Subgenome structure and expression reorganization and cooperation post hybridization and polyploidization are essential for speciation and allopolyploid success.However,the mechanisms have not yet been comprehensively assessed in animals.Here,we produced a high-fidelity reference genome sequence for common carp,a typical allotetraploid fish species cultured worldwide.This genome enabled in-depth analysis of the evolution of subgenome architecture and expression responses.Most genes were expressed with subgenome biases,with a trend of transition from the expression of subgenome A during the early stages to that of subgenome B during the late stages of embryonic development.While subgenome A evolved more rapidly,subgenome B contributed to a greater level of expression during development and under stressful conditions.Stable dominant patterns for homoeologous gene pairs both during development and under thermal stress suggest a potential fixed heterosis in the allotetraploid genome.Preferentially expressing either copy of a homoeologous gene at higher levels to confer development and response to stress indicates the dominant effect of heterosis.The plasticity of subgenomes and their shifting of dominant expression during early development,and in response to stressful conditions,provide novel insights into the molecular basis of the successful speciation,evolution,and heterosis of the allotetraploid common carp.

关键词： Allotetraploid Subgenome structural evolution Homoeologous expression Expression dominance shift Environmental adaptation

Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023 edition)

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Chinese Herbal Medicines 2024年第1期16卷 3-12页

作者： Qing Zhao Tong Wang Hongbin Wang Peng Cao chengyu Jiang Hongzhi Qiao lihua Peng Xingdong lin Yunyao Jiang Honglei Jin Huantian Zhang Shengpeng Wang Yang Wang Ying Wang Xi Chen Junbing Fan Bo li Geng li Bifeng liu Zhiyang li Suhua Qi Mingzhen Zhang Jianjian Zheng Jiuyao Zhou Lei Zheng Kewei Zhao Guangdong Engineering Research Center of Chinese Herbal Vesicles The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Institute of life and Health Engineering the First Affiliated HospitalJinan UniversityGuangzhou 510630China School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China College of Pharmacy Nanjing University of Chinese MedicineNanjing 210046China Institute of Basic Medicine Chinese Academy of Medical SciencesBeijing 100005China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310030China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China Department of Bone and Joint Surgery The First Affiliated Hospital of Jinan UniversityGuangzhou 510630China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoAomen 999078China Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai 200233China School of Basic Medical Sciences Southern Medical UniversityGuangzhou 510515China School of life Sciences Nanjing UniversityNanjing 210008China Nanfang Hospital Southern Medical UniversityGuangzhou 510515China Laboratory Animal Center Guangzhou University of Chinese MedicineGuangzhou 510405China College of life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital Nanjing 210008China School of Medical and Technology Xuzhou Medical UniversityXuzhou 221004China School of Basic Medical Sciences Xi’an Jiaotong UniversityXi’an 710065China The First Affiliated Hospital of Wenzhou Medical University WenZhou 325035China

To promote the development of extracellular vesicles of herbal medicine especially the establishment of standardization,led by the National Expert Committee on Research and Application of Chinese Herbal Vesicles,research experts in the field of herbal medicine and extracellular vesicles were invited nationwide with the support of the Expert Committee on Research and Application of Chinese Herbal Vesicles,Professional Committee on Extracellular Vesicle Research and Application,Chinese Society of Research Hospitals and the Guangdong Engineering Research Center of Chinese Herbal Vesicles.Based on the collation of relevant literature,we have adopted the Delphi method,the consensus meeting method combined with the nominal group method to form a discussion draft of“Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023)”.The first draft was discussed in online and offline meetings on October 12,14,November 2,2022 and April and May 2023 on the current status of research,nomenclature,isolation methods,quality standards and research applications of extracellular vesicles of Chinese herbal medicines,and 13 consensus opinions were finally formed.At the Third Academic Conference on Research and Application of Chinese Herbal Vesicles,held on May 26,2023,Kewei Zhao,convenor of the consensus,presented and read the consensus to the experts of the Expert Committee on Research and Application of Chinese Herbal Vesicles.The consensus highlights the characteristics and advantages of Chinese medicine,inherits the essence,and keeps the righteousness and innovation,aiming to provide a reference for colleagues engaged in research and application of Chinese herbal vesicles at home and abroad,decode the mystery behind Chinese herbal vesicles together,establish a safe,effective and controllable accurate Chinese herbal vesicle prevention and treatment system,and build a bridge for Chinese medicine to the world.

关键词： Chinese herbal medicine extracellular vesicles research application consensus

锰掺杂纳米羟基磷灰石的体外抗菌–促成骨性能研究

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无机材料学报 2024年第3期39卷 313-320页

作者：李承瑜丁自友韩颖超武汉理工大学材料复合新技术国家重点实验室武汉430070

羟基磷灰石(HAP)作为一种常见的骨修复材料,在治疗感染性骨缺损时仍面临着细菌感染的风险,有限的骨诱导能力也阻碍了其进一步应用。本研究采用共沉淀法制备了一种锰掺杂羟基磷灰石纳米棒(MnHAP),该纳米材料兼具优良的细胞生物相容性,高... 详细信息

羟基磷灰石(HAP)作为一种常见的骨修复材料,在治疗感染性骨缺损时仍面临着细菌感染的风险,有限的骨诱导能力也阻碍了其进一步应用。本研究采用共沉淀法制备了一种锰掺杂羟基磷灰石纳米棒(MnHAP),该纳米材料兼具优良的细胞生物相容性,高抗菌效率和骨诱导能力。抗菌实验表明,MnHAP10(n(Mn)/n(Ca+Mn)=10%)对大肠杆菌和金黄色葡萄球菌的抑制率可分别达到77.85%和75.92%,并且在808 nm近红外光照射下,对大肠杆菌的抗菌效率得到了进一步增强(97.63%)。细胞增殖以及相关成骨基因实验表明,MnHAP有利于成骨细胞的增殖分化,提高了对蛋白质的吸附能力和成骨活性,促进了相关成骨基因的表达,且具有良好的生物相容性。MnHAP纳米棒有望为感染性骨缺损治疗提供一种新的思路。

关键词：羟基磷灰石锰抗菌感染性骨缺损光热

Aerosol jet printing of surface acoustic wave microfluidic devices

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Microsystems & Nanoengineering 2024年第1期10卷 231-242页

作者： Joseph Rich Brian Cole Teng li Brandon Lu Hanyu Fu Brittany N.Smith Jianping Xia Shujie Yang Ruoyu Zhong James L.Doherty Kanji Kaneko Hiroaki Suzuki Zhenhua Tian Aaron D.Franklin tony Jun Huang Department of Biomedical Engineering Duke UniversityDurhamNC 27708USA Department of Electrical and Computer Engineering Duke UniversityDurhamNC 27708USA Department of Mechanical Engineering Virginia Polytechnic Institute and State UniversityBlacksburgVA 24061USA Thomas Lord Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNC 27708USA Deptartment of Precision Mechanics Faculty of Science and EngineeringChuo UniversityTokyo 112-8551Japan Department of Chemistry Duke UniversityDurhamNC 27708USA

The addition of surface acoustic wave(SAW)technologies to microfluidics has greatly advanced lab-on-a-chip applications due to their unique and powerful attributes,including high-precision manipulation,versatility,integrability,biocompatibility,contactless nature,and rapid actuation.However,the development of SAW microfluidic devices is limited by complex and time-consuming micro/nanofabrication techniques and access to cleanroom facilities for multistep photolithography and vacuum-based processing.To simplify the fabrication of SAW microfluidic devices with customizable dimensions and functions,we utilized the additive manufacturing technique of aerosol jet printing.We successfully fabricated customized SAW microfluidic devices of varying materials,including silver nanowires,graphene,and poly(3,4-ethylenedioxythiophene)polystyrene sulfonate(PEDOT:PSS).To characterize and compare the acoustic actuation performance of these aerosol jet printed SAW microfluidic devices with their cleanroom-fabricated counterparts,the wave displacements and resonant frequencies of the different fabricated devices were directly measured through scanning laser Doppler vibrometry.Finally,to exhibit the capability of the aerosol jet printed devices for lab-on-a-chip applications,we successfully conducted acoustic streaming and particle concentration experiments.Overall,we demonstrated a novel solution-based,direct-write,single-step,cleanroom-free additive manufacturing technique to rapidly develop SAW microfluidic devices that shows viability for applications in the fields of biology,chemistry,engineering,and medicine.

关键词： printing wave fluid

High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping:FLOAT

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Microsystems & Nanoengineering 2024年第1期10卷 313-323页

作者： Joseph Rufo Peiran Zhang Zeyu Wang Yuyang Gu Kaichun Yang Joseph Rich Chuyi Chen Ruoyu Zhong Ke Jin Ye He Jianping Xia Ke li Jiarong Wu Yingshi Ouyang Yoel Sadovsky Luke P.Lee tony Jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Obstetrics Gynecologyand Reproductive SciencesMagee-Womens Research InstituteUniversity of PittsburghPittsburghPAUSA Department of Microbiology and Molecular Genetics School of MedicineUniversity of PittsburghPittsburghPAUSA Renal Division and Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Department of Bioengineering Department of Electrical Engineering and Computer ScienceUniversity of CaliforniaBerkeleyBerkeleyCAUSA Department of Biophysics Institute of Quantum BiophysicsSungkyunkwan UniversitySuwonKorea

Extracellular vesicles(EVs)have been identified as promising biomarkers for the noninvasive diagnosis of various diseases.However,challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low purities.Here,we report a high-yield(>90%)and rapid(lizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid droplets.By adding a thermoresponsive polymer flocculant,nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the droplet.We demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein,addressing a significant obstacle in the development of EV-based liquid biopsies.Due to its high-yield nature,FLOAT reduces biofluid starting volume requirements by a factor of 100(from 20 mL to 200µL),demonstrating its promising potential in point-of-care diagnostics.

关键词： trapping utilize separating