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LncRNA DPP10-AS1 promotes malignant processes through epigenetically activating its cognate gene DPP10 and predicts poor prognosis in lung cancer patients

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Cancer Biology & Medicine 2021年第3期18卷 675-692页

作者： Haihua Tian Jinchang Pan Shuai Fang chengwei Zhou Hui Tian Jinxian He Weiyu shen Xiaodan Meng Xiaofeng Jin Zhaohui Gong Department of Biochemistry and Molecular Biology Ningbo University School of MedicineNingbo 315211China Zhejiang Province Key Laboratory of Pathophysiology Ningbo University School of MedicineNingbo 315211China Department of Thoracic Surgery The Affiliated Hospital of Ningbo University School of MedicineNingbo 315020China Department of Thoracic Surgery The Affiliated Lihuili Hospital of Ningbo UniversityNingbo 315048China

Objective:The purpose of this study was to explore the function and gene expression regulation of the newly identified lnc RNA DPP10-AS1 in lung cancer,and its potential value as a prognostic ***:q RT-PCR and Western blot were conducted to detect the expression of DDP10-AS1 and DPP10 in lung cancer cell lines and *** effects of DDP10-AS1 on DPP10 expression,cell growth,invasion,apoptosis,and in vivo tumor growth were investigated in lung cancer cells by Western blot,rescue experiments,colony formation,flow cytometry,and xenograft animal ***:The novel antisense lnc RNA DPP10-AS1 was found to be highly expressed in cancer tissues(P<0.0001),and its upregulation predicted poor prognosis in patients with lung cancer(P=0.0025).Notably,DPP10-AS1 promoted lung cancer cell growth,colony formation,and cell cycle progression,and repressed apoptosis in lung cancer cells by upregulating DPP10 ***,DPP10-AS1 facilitated lung tumor growth via upregulation of DPP10 protein in a xenograft mouse ***,DPP10-AS1 positively regulated DPP10 gene expression,and both were coordinately upregulated in lung cancer ***,DPP10-AS1 was found to associate with DPP10 m RNA but did not enhance DPP10 m RNA *** of DPP10-AS1 and DPP10 contributed to their coordinate upregulation in lung ***:These findings indicated that the upregulation of the antisense lnc RNA DPP10-AS1 promotes lung cancer malignant processes and facilitates tumorigenesis by epigenetically regulating its cognate sense gene ***10-AS1 may serve as a candidate prognostic biomarker and a potential therapeutic target in lung cancer.

关键词： Antisense long noncoding RNA DPP10-AS1 hypomethylation malignant process lung cancer

HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation

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Molecular Plant 2020年第3期13卷 515-531页

作者： Chao Yang Wenjin shen Lianming Yang Yun Sun Xibao Li Minyi Lai Juan Wei Chaojun Wang Yingchao Xu Faqiang Li Shan Liang chengwei Yang Shangwei Zhong Ming Luo Caiji Gao Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Applied Botany Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic ImprovementSouth China Botanical GardenCore Botanical GardensChinese Academy of SciencesGuangzhou 510650China College of Life Sciences Leshan Normal UniversityLeshan 614004China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China School of Advanced Agricultural Sciences and School of Life Sciences Peking University100871 BeijingChina Center of Economic Botany Core Botanical GardensChinese Academy of SciencesGuangzhou 510650China These authors contributed equally to this article

Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development,but the molecular link between light signaling and the autophagy pathway has not been elucidated in *** this study,we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes(ATGs).We showed that depletion of the ELONGATED HYPOCOTYL 5(HY5),a key component of light signaling,leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments,contributing to higher expression ***5 interacts with and recruits HISTONE DEACETYLASE 9(HDA9)to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone ***,we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci,leading to their depression and thereby activated *** analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy ***,our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants.

关键词： autophagy histone acetylation HY5 HDA9 light-to-dark shift nitrogen starvation

HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation

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Molecular Plant 2022年第10期15卷 1632-1634页

作者： Chao Yang Wenjin shen Lianming Yang Yun Sun Xibao Li Minyi Lai Juan Wei Chaojun Wang Yingchao Xu Faqiang Li Shan Liang chengwei Yang Shangwei Zhong Ming Luo Caiji Gao 不详

(Molecular Plant 13,515-531;March 2020)After publication of our original manuscript,we became aware of errors in Figure *** the preparation of Figure 4C in this article as originally published,we inadvertently duplicated the image of hda9-1(MS-N)as that of the hda9-1-C(MS+N).Also,in Figure 4H,the image of pUBQ10:GFP-ATG8a/hda9-1(MS+L)was mistakenly a duplicate of pUBQ10:GFP-ATG8a/WT(MS+L)shown in Figure S7C.A corrected version of Figure 4 is shown *** scientific conclusions of this article have not been affected by this *** authors apologize for not detecting this error prior to publication and for any inconvenience that may have been caused.

关键词： Figure Plant Conversion

Facile preparation of silver nanoparticles in bulk silicate glass by high-repetition-rate picosecond laser pulses

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Chinese Optics Letters 2021年第1期19卷 75-79页

作者： Danyang shen Jing Qian chengwei Wang Guande Wang Xuehui Wang Quanzhong Zhao State Key Laboratory of High Field Laser Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Technology Center Huagong Laser Engineering Co.Ltd.Wuhan 430000China

One-step precipitation of Ag nanoparticles in Ag+-doped silicate glasses was achieved through a focused picosecond laser with a high repetition rate. Absorption spectra and transmission electron microscopy(TEM) confirmed that metallic Ag nanoparticles were precipitated within glass samples in the laser-written domain. The surface plasmon absorbance fits well with the experimental absorption spectrum. The nonlinear absorption coefficient β is determined to be 2.47 × 10-14 m/W by fitting the open aperture Z-scan curve, which originated from the intraband transition in the s-p Ag band. The formation mechanism of Ag-glass nanocomposites is discussed as well.

关键词： nonlinear optical materials laser materials processing microstructure fabrication

Tunable THz spectrum analyzer with hyperspectral resolution

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Chinese Optics Letters 2024年第7期22卷 111-116页

作者： Xin He Mingjia Yao chengwei Qian Leixin Meng Xiaowei Liu Wenhao Tang Xiaoming shen Paul Beckett Duo Xiao Xu Liu Rui Yin School of Information and Electrical Engineering Hangzhou City UniversityHangzhou 310015China Research Center for Humanoid Sensing Zhejiang LabHangzhou 311121China State Key Laboratory of Modern Optical Instrumentation College of Optical Science and EngineeringZhejiang UniversityHangzhou 310027China Research Center for Intelligent Chips and Devices Zhejiang LabHangzhou 311121China STEM College RMIT UniversityMelbourneVIC 3000Australia

We present a tunable terahertz(THz)spectrum analyzer with hyperspectral resolution formed from electrically tunable metamaterial and plasmonic *** few as eight encoders based on four detectors are needed to recover 396 spectral *** incident spectra in the range of 1–5 THz can be reconstructed with a localization precision of 0.3 GHz and a minimum average mean squared error(MSE)of 6.9×10^(−5).Our proposed analyzers are faster and more portable than those based on frequency combs and power meters,and more accurate than existing Fourier transform techniques,showing promising applications in pathology,biomedical imaging,and many other areas.

关键词： tunable metamaterial plasmonics spectrum analyzer

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水位波动条件下盾构极限支护压力半解析研究

上海交通大学学报 2018年第8期52卷 982-990页

作者：应宏伟沈华伟张金红朱成伟浙江大学滨海和城市岩土工程研究中心软弱土与环境土工教育部重点实验室杭州310058 浙江省建筑设计研究院杭州310008

为研究潮汐作用对盾构隧道开挖的影响,采用有限元软件COMSOL建立三维数值模型,得到水位波动条件下盾构隧道开挖面前的渗流场变化规律,发现粉土地基中的渗流存在幅值衰减与相位滞后现象.将计算所得渗透力应用到"楔形体—棱柱体"极限平衡... 详细信息

为研究潮汐作用对盾构隧道开挖的影响,采用有限元软件COMSOL建立三维数值模型,得到水位波动条件下盾构隧道开挖面前的渗流场变化规律,发现粉土地基中的渗流存在幅值衰减与相位滞后现象.将计算所得渗透力应用到"楔形体—棱柱体"极限平衡模型中,计算并分析开挖面极限支护压力在水位波动条件下的变化特征,发现极限支护压力的波动与隧道埋深比、土层性质、边界水位波动等因素相关.随着隧道埋深比的增大和波动周期的减小,极限支护力的幅值衰减与相位滞后更为显著.潮汐作用下的极限支护压力最大值比最高潮位稳态渗流情况要小,支护力的最大值与最高潮位出现的时间不一致.

关键词：水位波动盾构开挖面极限支护压力有限元法半解析解

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复杂环境下污水压力输送管道的工程实践

环境工程 2023年第S2期41卷 951-954页

作者：沈浩杨成慰史成波中国市政工程中南设计研究总院有限公司湖北武汉430010

本文以汕头市黄厝围泵站至新溪污水处理厂的污水压力输送管道设计为例,主要分析了高重要性污水压力输水管道同时穿越道路立交、高速公路、现状铁路、待建铁路和通航河道等复杂环境的穿越方式和设计要点。对铁路隧道交叉位置设计刚性支... 详细信息

本文以汕头市黄厝围泵站至新溪污水处理厂的污水压力输送管道设计为例,主要分析了高重要性污水压力输水管道同时穿越道路立交、高速公路、现状铁路、待建铁路和通航河道等复杂环境的穿越方式和设计要点。对铁路隧道交叉位置设计刚性支座以保护管道稳定,待铁路隧道盾构穿越完成后,管道基础未发生变形沉降,防护效果较好。此外,为保障污水压力输水管道的近、远期安全运行,对此污水压力管道进行了阴极保护设计及水锤模拟分析,同时考虑检修阀组分区设计,以保障管道事故状态下的通水能力。目前管道已建成运行超过1年,运行效果良好,表明方案合理可行,可为类似工程提供经验借鉴。

关键词：污水输水压力管复杂环境刚性支座阴极保护水锤防护

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单轴压缩下煤岩细观结构参数表征及演化规律

煤炭科学技术 2023年第4期51卷 88-95页

作者：杨琪于岩斌崔文亭高成伟张鑫申家龙山东科技大学矿山灾害预防控制省部共建国家重点实验室培育基地山东青岛266590 山东科技大学安全与环境工程学院山东青岛266590

为研究煤岩细观结构特征随着轴向应力增加的演化规律,利用NanoVoxel-3502E X射线三维显微镜与Deben原位加载实验台进行煤岩单轴压缩试验,获取不同应变条件下的CT数据,并结合三维可视化软件AVIZO,研究煤样试件的孔隙裂隙分布特征,统计分... 详细信息

为研究煤岩细观结构特征随着轴向应力增加的演化规律,利用NanoVoxel-3502E X射线三维显微镜与Deben原位加载实验台进行煤岩单轴压缩试验,获取不同应变条件下的CT数据,并结合三维可视化软件AVIZO,研究煤样试件的孔隙裂隙分布特征,统计分析煤样试件孔隙裂隙细观参数-球形度、配位数、曲率和开度。揭示了煤样在单轴压缩情况下的细观结构空间分布特征及随时间的展布规律,细观结构随轴向应力的演化规律。研究表明:微小裂隙在20~732 N过程中大量出现,孔隙增多,在431~732 N过程中孔隙球形度集中分布范围变窄,部分孔裂隙逐渐融合进入裂隙网络,部分孔裂隙被压实压密,732 N时部分孔隙被压实压密,导致孔裂隙减少90000个,裂隙曲率逐渐增大、裂隙逐渐开度变大,配位数集中率更高,同时也说明煤样内部连通性更好,732 N是裂隙网络发育的巅峰。轴向力达到1100 N(峰值)时,形成复杂的裂隙网络。随着单轴压缩过程的推进,部分孔裂隙虽然在732 N被压实压密,但由于轴向力的增大以及残余荷载的作用,孔裂隙的增多是必然趋势,峰值和峰后仍然存在微小孔裂隙,但峰后裂隙较窄,球形度更多的是集中在1.5~2.0。贯通裂隙的曲率在裂隙网络的边缘普遍较高,即裂隙网络边缘为裂隙发育带。为研究煤样的时空演化规律提供了一种可行的技术手段,对于阐释煤岩细观结构时空演化过程具有实际意义。

关键词： CT 单轴压缩原位测试细观结构煤结构细观结构参数

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PON系统承载TDM技术研究

电信科学 2010年第8期26卷 14-18页

作者：王成巍沈成彬蒋铭中国电信股份有限公司上海研究院上海200122

作为综合接入系统,PON系统不仅要能承载数据、视频和语音等业务,还应能承载TDM业务。本文结合TDM业务的传输特性,研究了TDM over EPON/GPON的各种电路仿真协议,提出了TDM over EPON/GPON的协议参考模型,详细分析了PON系统承载TDM业务的... 详细信息

作为综合接入系统,PON系统不仅要能承载数据、视频和语音等业务,还应能承载TDM业务。本文结合TDM业务的传输特性,研究了TDM over EPON/GPON的各种电路仿真协议,提出了TDM over EPON/GPON的协议参考模型,详细分析了PON系统承载TDM业务的关键技术。

关键词：无源光网络 TDM电路仿真 MEF8 边缘到边缘的伪线仿真 Native TDM