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Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

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World Journal of Stem Cells 2024年第3期16卷 305-323页

作者： jian-Jing Lin Tong Ning Shi-cheng jia Ke-jia Li Yong-Can Huang Qiang Liu jian-Hao Lin Xin-Tao Zhang Department of Sports Medicine and Rehabilitation Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Medical Science The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250033Shandong ProvinceChina Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijing 100871China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine ***,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple ***,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs *** To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with *** The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome *** were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA *** transcript was normalized by fragments per kilobase per *** change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction *** In total,263 DEGs were discovered,with 92 upregulated and 171 *** were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound *** cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and *** aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid *** real-time polymerase chain reaction confirmed targeted transcript *** Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 26

关键词： Nanosecond pulsed electric fields Whole transcriptome sequencing Mesenchymal stem cells Genetic response Stem cell engineering

Tailoring Variant Pairing to Enhance Impact Toughness in High-Strength Low-Alloy Steels via Trace Carbon Addition

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Acta Metallurgica Sinica(English Letters) 2021年第6期34卷 755-764页

作者： Yi-Shuang Yu Zhi-Quan Wang Bin-Bin Wu Jing-Xiao Zhao Xue-Lin Wang Hui Guo cheng-jia Shang Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China Key Laboratory for Advanced Materials of Ministry of Education School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China State Key Laboratory of Metal Material for Marine Equipment and Application Anshan 114021China

Alloying can make conventional metals reach ultra-high strength,but this usually comes at dramatic loss of *** this work,a desirable strength–toughness combination in high-strength low-alloy steel achieved via trace carbon *** significance of carbon in tailoring variant pairing and tuning impact toughness was elucidated from the perspective of crystallography and *** the carbon content increases,the packets and blocks are refined,and the-40 impact toughness *** enhancement of impact toughness results from the higher density of block boundaries,and the fracture mode shifts from brittle fracture to ductile–brittle combined fractures,then to ductile fracture due to the increased *** the carbon content would lower the martensite start temperature(M_S)temperature and driving force for martensitic transformation,and increase the strength of austenite matrix,which in turn contributes to producing more V1/V2 variant pairs to accommodate the transformation strain.

关键词： High-strength low-alloy steel Carbon Impact toughness Variant pairing Phase transformation

AP-1 and SP1 trans-activate the expression of hepatic CYP1A1 and CYP2A6 in the bioactivation of AFB1 in chicken

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Science China(Life Sciences) 2024年第7期67卷 1468-1478页

作者： jiang Deng jia-cheng Yang Yue Feng Ze-Jing Xu Kamil Kuča Meng Liu Lv-Hui Sun State Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070China Department of Chemistry Faculty of ScienceUniversity of Hradec KraloveHradec Kralove 50003Czech Republic

Dietary exposure to aflatoxin B1(AFB1)is harmful to the health and performance of domestic *** hepatic cytochrome P450s(CYPs),CYP1A1 and CYP2A6,are the primary enzymes responsible for the bioactivation of AFB1to the highly toxic exo-AFB1-8,9-epoxide(AFBO)in ***,the transcriptional regulation mechanism of these CYP genes in the liver of chicks in AFB1metabolism remains ***-luciferase reporter assay,bioinformatics and site-directed mutation results indicated that specificity protein 1(SP1)and activator protein-1(AP-1)motifs were located in the core region-1,063/-948,-606/-541 of the CYP1A1 promoter as well as-636/-595,-503/-462,-147/-1 of the CYP2A6 ***,overexpression and decoy oligodeoxynucleotide technologies demonstrated that SP1 and AP-1 were pivotal transcriptional activators regulating the promoter activity of CYP1A1 and ***,bioactivation of AFB1to AFBO could be increased by upregulation of CYP1A1 and CYP2A6 expression,which was trans-activated owing to the upregulation of AP-1,rather than SP1,stimulated by AFB1-induced reactive oxygen ***,nano-selenium could reduce ROS,downregulate AP-1 expression and then decrease the expression of CYP1A1 and CYP2A6,thus alleviating the toxicity of *** conclusion,AP-1 and SP1 played important roles in the transactivation of CYP1A1 and CYP2A6 expression and further bioactivated AFB1to AFBO in chicken liver,which could provide novel targets for the remediation of aflatoxicosis in chicks.

关键词： aflatoxin B1 CYP1A1 CYP2A6 SP1 AP-1 transcriptional activation

Seven New Sesquiterpene Lactones from Eupatorium chinense

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Chinese Journal of Chemistry 2005年第11期23卷 1530-1536页

作者： YANG, Sheng-Ping cheng, jia-Gao HUO, Jun jiaNG, Hua-Liang CHEN, Kai-Xian YUE, jian-Min State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Zhangjiang Hi-Tech Park Shanghai 201203 China

Seven new sesquiterpenoids, namely eupatochinilides Ⅰ-Ⅶ （1-7）, together with eight known compounds, euponin （8）, mollisorin A （9）, niveusin B （10）, 8β-（4＇-acetoxy-tiglyloxy）-3β-hydroxy-6Hβ,7Hα-germacra-1（10）E,4E,11（13）-trien-6,12-olide （11）, eupalinifide B （12）, 8β-（4＇-hydroxytigloyloxy）-5-desoxy-8-desacyleuparotin （13）, 3-deacetyeupalinin A （14）, and 15-hydroxyleptocarpin （15）, were isolated from the ethanolic extract of the whole plant of Eupatorium chinense L. Their structures and stereochemistry were established by spectroscopic methods and GIAO based ^13C NMR chemical shift calculations.

关键词： Eupatorium chinense sesquiterpenoid eupatochinilides Ⅰ-Ⅶ gauge-independent atomic orbital,quantum chemical calculation

Triclosan-induced Oxidative Stress Injury and Apoptosis by Regulating the PI3K/Akt/Caspase-3 Signaling Pathway in Human Renal Glomerular Endothelial Cells

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Biomedical and Environmental Sciences 2022年第6期35卷 547-551页

作者： MA Yan CHEN Chen WANG Jing Bo cheng jia Li SHEN Shi CHEN Xi HUO Jun Sheng Key Laboratory of Trace Element Nutrition National Institute for Nutrition and HealthChinese Center for Disease Control and PreventionBeijing 100050China

Triclosan(TCS)is widely used in personal-care products because of its bactericidal and antibacterial ***,TCS and its toxic byproducts have been detected in aquatic environments,animals,and plants *** is a common phenolic environmental endocrine disruptor,and TCS in the environment enters the body mainly through diet and *** of the absorbed TCS is metabolized by the human kidneys and is eliminated in the urine[1].Some epidemiological studies have suggested a positive correlation between the TCS exposure level in urine and albumin(a biomarker of renal function),which could cause renal dysfunction[2].However,the potential effects of TCS on the mammalian kidney and its mechanisms are currently ***,we initiated an in vitro trial to study the molecular mechanism of cytotoxicity of TCS to glomeruli.

关键词： PI3K/Akt urine Apoptosis

Unsteady flow structures in centrifugal pump under two types of stall conditions

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Journal of Hydrodynamics 2018年第6期30卷 1038-1044页

作者： Pei-jian Zhou jia-cheng Dai Ya-fei Li Ting Chen Jie-gang Mou College of Mechanical Engineering Zhejiang University of Technology Engineering Research Center of Process Equipment and Remanufacturing Ministry of Education School of Science Wuhan Institute of Technology

The stall is an unsteady flow phenomenon that always causes instabilities and low efficiency for pumps. This paper focuses on the unsteady flow structures and evolutions under two types of stall conditions in centrifugal pump impellers. Two centrifugal pump impellers, one with 6 and another with 5 blades, are considered and a developed large-eddy simulation method is adopted. The results show that the alternative stall occurs in the impeller with 6 blades, while, the rotating stall is observed in that with 5 blades. The flow structure and the pressure fluctuation characteristics are further analyzed. For the alternative stall, the stall cells are fixed relative to the impeller, but a large vortex in the stalled passage is always swaying. The outlet vortex is generated from it, and then develops and sheds periodically. For the rotating stall, the stall cells first occur in the suction side of the blade. With the growth of the stall cells, the block area gradually increases until the inlet region is almost blocked, then moves to the pressure side with a continuous decay. When the rotating stall occurs, the amplitude of the pressure fluctuation is much larger than that under the alternative stall condition. The propagation of the stall cells has a significant effect on the pressure fluctuations in the impeller.

关键词： Centrifugal pump flow structures rotating stall alternative stall large-eddy simulation

Landslides Triggered by the 2020 Qiaojia M_(w)5.1 Earthquake, Yunnan, China: Distribution, Influence Factors and Tectonic Significance

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Journal of Earth Science 2021年第5期32卷 1056-1068页

作者： Xiangli He Chong Xu Wenwen Qi Yuandong Huang jia cheng Xiwei Xu Qi Yao Yongkun Lu Boyang Dai National Institute of Natural Hazards Ministry of Emergency Management of ChinaBeijing 100085China Key Laboratory of Compound and Chained Natural Hazards Dynamics(Under Construction) Ministry of Emergency Management of ChinaBeijing 100085China China University of Geosciences Beijing 100083China China Earthquake Networks Center Beijing 100045China Yunnan Earthquake Agency Kunming 650224China

On May 18, 2020, an M_(w)5.1 earthquake occurred in Qiaojia County, Yunnan Province, China. This moderate-sized event triggered massive coseismic landslides, resulting in some damage. In this work, through visual interpretation of high-resolution(0.8–2 m) Gaofen satellite images before and after the earthquake, 167 landslides were delineated, 18 of which were inspected in the field. Using the landslide number density(LND) and landslide area percentage(LAP), we characterized the spatial distribution of these landslides, and analyzed their possible influence factors and tectonic significance. The results show that these landslides are distributed mostly in the NW-SE direction, roughly parallel to the long axis of seismic intensity zones and the strike of the Xiaohe-Baogunao fault(XBF). The LND and LAP decrease with increasing distances to the fault and from the epicenter to fault ends of the XBF. These permit to suggest that the seismogenic fault of the Qiaojia earthquake is likely a hidden branch of the XBF. All of the landslides induced by this event occurred in the region with the seismic intensity of six degrees or greater of the 2014 M_(w)6.2 Ludian earthquake. Therefore, it was inferred that the 2020 Qiaojia earthquake was probably the subsequent release of accumulated elastic strain after the 2014 Ludian earthquake in a same tectonic stress regime.

关键词： Qiaojia earthquake landslide inventory spatial distribution seismogenic fault Ludian earthquake

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Clinical diagnosis,treatment,and medical identification of specific pulmonary infection in naval pilots:Four case reports

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World Journal of Clinical Cases 2022年第16期10卷 5487-5494页

作者： jia Zeng Guo-Li Zhao jia-cheng Yi Dan-Dan Liu Yan-Qing jiang Xiang Lu Yan-Bing Liu Fei Xue Jie Dong Department of Aviation Disease Naval Medical Center of PLAShanghai 200052China Department of Radiology Naval Medical Center of PLAShanghai 200052China Department of Clinical Medicine School of Basic MedicineHebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina Department of Respiratory Medicine Changhai HospitalNaval Military Medical UniversityShanghai 200081China

BACKGROUND Specific pulmonary infection could seriously threaten the health of pilots and their *** consequences are *** investigated the clinical diagnosis,treatment,and medical identification of specific pulmonary infections in naval *** SUMMARY We analyzed the medical waiver and clinical data of four pilots with specific pulmonary infections,who had accepted treatment at the Naval Medical Center of Chinese People’s Liberation Army between January 2020 and November 2021,including three cases of tuberculosis and one of cryptococcal *** cases underwent a series of comprehensive treatment *** cases successfully obtained medical waiver for flight after being cured,while one was grounded after reaching the maximum flight life after being *** Chest computed tomography scanning should be used instead of chest radiography in pilots’physical *** pilots with specific pulmonary infection can be cured and return to flight.

关键词： Cryptococcal pneumonia Tuberculosis Diagnosis Treatment Medical identification Pilot Pulmonary infection Case report

Laparoscopic extended right hepatectomy for posterior and completely caudate massive liver tumor (with videos)

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Hepatobiliary & Pancreatic Diseases International 2023年第3期22卷 326-330页

作者： Liang Chen Lu-Zheng Liu jia-cheng Chen Da-Feng Xu cheng Chen Shi-Xun Lin Xiang-Xiang Luo Jin-Cai Wu Division of Hepatobiliary Pancreatic Surgery Hainan Affiliated Hospital of Hainan Medical University(Hainan General Hospital)Haikou 570311China

To the Editor:Liver tumor may occur in any hepatic segment or lobe,and thus the liver resection is individualized as per the location and size of the *** addition,the resection of the posterior and caudate lobes of the liver is especially difficult amongst all types of *** et *** that the laparoscopic resection of right posterior liver lobe was a difficult surgical procedure[1].

关键词： hepatectomy liver