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Active DNA demethylation regulates MAMP-triggered immune priming in Arabidopsis

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Journal of Genetics and Genomics 2022年第8期49卷 796-809页

作者： Mengling Huang Ying Zhang Ying Wang Jiatao Xie jiasen cheng Yanping Fu Daohong Jiang Xiao Yu Bo Li State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhanHubei 430070China The Provincial Key Lab of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan Laboratory WuhanHubei 430070China

Plants recognize microbe-associated molecular patterns(MAMPs)to activate immune responses and defense priming to defend against pathogen *** regulation of gene expression is crucial for plant immunity and is mediated by multiple factors,including DNA ***,it remains unknown whether and how DNA demethylation contributes to immune responses in MAMPtriggered ***,we report that active DNA demethylation is required for MAMP-triggered immunity to bacterial *** rdd-2 triple mutant carrying mutations in ROS1,DML2,and DML3 that encode DNA glycosylases,which are key DNA demethylation enzymes,exhibits compromised immune responses triggered by the MAMPs fig22 and ***-wide methylome analysis reveals that fig22 induces rapid and specific DNA demethylation in an RDD-dependent *** expression levels of salicylic acid signaling-related and phytoalexin biosynthesis-related genes are tightly associated with the fig22-induced promoter *** compromised accumulation of priming compounds and antimicrobial metabolites ultimately leads to a defense priming defect in the rdd-2 *** results reveal the critical role of active DNA demethylation in the MAMP-triggered immune response and provide unique insight into the molecular mechanism of fig22-modulated DNA demethylation.

关键词： Pattern-triggered immunity Immune priming Defense gene expression DNA demethylation Immune signal transduction

Fusarivirus accessory helicases present an evolutionary link for viruses infecting plants and fungi

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Virologica Sinica 2022年第3期37卷 427-436页

作者： Assane Hamidou Abdoulaye Jichun Jia Aqleem Abbas Du Hai jiasen cheng Yanping Fu Yang Lin Daohong Jiang Jiatao Xie State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan430070China Hubei Key Laboratory of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Hubei Hongshan Laboratory Wuhan430070China

A significant number of mycoviruses have been identified that are related to plant viruses,but their evolutionary relationships are largely unexplored.A fusarivirus,Rhizoctonia solani fusarivirus 4(RsFV4),was identified in phytopathogenic fungus Rhizoctonia solani(***)strain XY74 co-infected by an ***4 had a genome of 10,833 nt(excluding the poly-A tail),and consisted of four non-overlapping open reading frames(ORFs).ORF1 encodes an 825 aa protein containing a conserved helicase domain(Hel1).ORF3 encodes 1550 aa protein with two conserved domains,namely an RNA-dependent RNA polymerase(RdRp)and another helicase(Hel2).The ORF2 and ORF4 likely encode two hypothetical proteins(520 and 542 aa)with unknown *** phylogenetic analysis based on Hel2 and RdRp suggest that RsFV4 was positioned within the fusarivirus group,but formed an independent branch with three previously reported fusariviruses of ***,the Hel1 and its relatives were phylogenetically closer to helicases of potyviruses and hypoviruses than fusariviruses,suggesting fusarivirus Hel1 formed an evolutionary link between these three virus *** finding provides evidence of the occurrence of a horizontal gene transfer or recombination event between mycoviruses and plant viruses or between *** findings are likely to enhance the understanding of virus evolution and diversity.

关键词： Fusarivirus Potyvirus Hypovirus Helicase Horizontal gene transfer(HGT)

Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

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Stress Biology 2022年第1期2卷 299-311页

作者： Mirza Abid Mehmood Yanping Fu Huizhang Zhao jiasen cheng Jiatao Xie Daohong Jiang State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China Plant Pathology Institute of Plant ProtectionMNS University of AgricultureMultan 60000Pakistan Provincial Key Laboratory of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityHubei ProvinceWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China

Rice sheath blight pathogen,Rhizoctonia solani,produces numerous sclerotia to *** a rich source of nutrients in the soil,sclerotia may lead to the change of soil *** this purpose,we amended the sclerotia of *** in soil and analyzed the changes in bacterial microbiota within the soil at different time *** the phyla level,Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Chloroflexi and Firmicutes showed varied abundance in the amended soil samples compared to those in the *** increased abundance of ammonia-oxidizing bacterium(AOB)Nitrosospira and Nitrite oxidizing bacteria(NOB)i.e.,Nitrospira was observed,where the latter is reportedly involved in the nitrifier ***,Thiobacillus,Gemmatimonas,Anaeromyxobacter and Geobacter,the vital players in denitrification,N2O reduction and reductive nitrogen transformation,respectively,depicted enhanced abundance in *** sclerotia-amended ***,asymbiotic nitrogen-fixing bacteria,notably,Azotobacter as well as Microvirga and Phenylobacterium with nitrogen-fixing potential also enriched in the amended samples compared to the *** growth promoting bacteria,such as Kribbella,Chitinophaga and Flavisolibacter also enriched in the sclerotia-amended *** per our knowledge,this study is of its kind where pathogenic fungal sclerotia activated microbes with a potential role in N transformation and provided clues about the ecological functions of *** sclerotia on the stimulation of bacterial genera involved in different processes of N-cycle within the soil in the absence of host plants.

关键词： Rice sheath blight Rhizoctonia solani Sclerotia Reductive nitrogen transformation Nitrogen fixation Soil microbiome

A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

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Molecular Plant 2020年第10期13卷 1420-1433页

作者： Hongxiang Zhang Jiatao Xie Yanping Fu jiasen cheng Zheng Qu Zhenzhen Zhao Shufen cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge Daohong Jiang State Key Laboratory of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei Province 430070China The Provincial Key Lab of Plant Pathology of Hubei Province Huazhong Agricultural UniversityWuhanChina Department of Plant and Environmental Sciences University of Copenhagen1871 Frederiksberg CDenmark

Mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal ***,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop *** this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic ***-1 downregulates the expression of key pathogenicity factor genes in *** during *** growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm *** a result,plant growth is promoted and disease resistance is *** experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other *** strains on DT-8-inoculated plants and that DT-8 could be recovered from dead *** findings suggest that the mycoviruses may have the ability to shape the origin of *** discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

关键词： mycovirus Sclerotinia sclerotiorum endophyte rapeseed stem rot biological control Brassica napus

Enrichment of beneficial and suppression of pathogenic microorganisms in response to the addition of Sclerotinia sclerotiorum sclerotia in soil and its effect on microbial diversity

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Enrichment of beneficial and suppression of pathogenic micro...

Botrytis cinerea type Ⅱ IAP BcBIR1 improves the biocontrol capacity of Coniothyrium minitans

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Mirza Abid Mehmood Xie Jiatao cheng jiasen Fu Yanping Jiang Daohong The State Key Laboratory of Agriculture Microbiology Huazhong Agricultural University The Key Laboratory of Plant Pathology of Hubei Province Huazhong Agricultural University

Soil microbiome plays an imperative role due to their multifunctional activities including suppression of soil-borne *** sclerotiorum is a notorious soil-borne pathogen of various important crops and produce sclerotia to survive within soil for long *** is enriched in carbohydrates,fats,proteins and chitin which can be utilized by microbes to obtain nutrients required for their optimum *** in view the sclerotia as a nutritional source for microbes,we hypothesized that sclerotia in soil may affect the microbial diversity directly or/and *** this study,we incubated sclerotia of *** in soil collected from field to observe shift-in microbial diversity across three months using 16S rRNA and ITS sequencing *** observed surprising shift-in microbial diversity with non-amended soil having more diverse microbes than sclerotia-amended soil *** diversity indices confirmed the decline in diversity of sclerotia-amended soil samples of bacterial and fungal communities with the exception of 3 month in fungal community where more diverse community was observed in sclerotia-amended soil samples compared to the non-amended ***-amended soil activated the antagonists and plant growth promoting microbes whereas,several plant-pathogenic fungi depicted decline in their abundances with the passage of ***,bacteria with potential role in plant growth promotion showed enhanced abundance in sclerotia-amended soils,such as Burkholderia,Chitinophaga and Dyella ***,fungal diversity exhibited enrichment of fungi with reported antagonistic activities such as,Clonostachys,Trichoderma and Talaromyces *** the contrary,we observed a drastic decline in abundance of numerous notorious plant-pathogenic fungi such as,Alternaria alternata,Botrytis cinerea,Fusarium graminearum,***,***,*** and Sclerotiumrolfsii *** sclerotia-amended soil ***,we conclude that

关键词： Enrichment Suppression Microbiome Sclerotinia sclerotiorum Sclerotia 16S rRNA ITS

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Botrytis cinerea type Ⅱ IAP BcBIR1 improves the biocontrol ...

中国植物病理学会2021年学术年会

作者： WU Jianing ZHANG Hongxiang JIANG Daohong FU Yanping XIE Jiatao cheng jiasen LIN Yang State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University The Provincial Key Lab of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural University

Apoptosis-like programmed cell death（A-PCD） is associated with fungal development,aging,pathogenicity,and stress ***,to determine whether the Botrytis cinerea type Ⅱ inhibitor of apoptosis（IAP）,BcBIR1,could improve Coniothyrium minitans biocontrol capability,BcBIR1 gene was expressed in *** strain ***1-expressed strains Bcbir1-12 and Bcbir1-17 were randomly selected to determine the transcriptional profiles of BcBIR1 and its *** homolog,CmBIR1,during the conidial germination,hyphal growth,and pycnidial formation ***1 expression did not significantly impact CmBIR1 expression in strains Bcbir1-12 and Bcbir1-17,which exhibited higher rates of(onidiation,mycelial growth,and biomass growth than their parent strain *** diverse abiotic stresses,such as nutrient deficiency and exogenous H202 treatment,the growth-inhibiting rate of transgenic strains was significantly lower than that of the wildtype *** conidial survival rate of strains Bcbir1-12 and Bcbir1-17 treated with ultraviolet（UV） irradiation was also higher than that of strain *** a confrontation experiment,BcBIR1 expression enhanced *** mycoparasitism toward Sclerotinia sclerotiorum *** together,the results suggest that BcBIR1 positively regulates the vegetative growth,conidiation,resistance to abiotic stress,and parasitic ability of ***,as a potential synergistic gene,BcBIR1 may improve *** control capacity against ***.

关键词： BcBIR1 Type Ⅱ IAP Coniothyrium minitans Biocontrol

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外圆磨床砂轮架的振动分析

南京林业大学学报(自然科学版) 1986年第3期 131-138页

作者：程嘉森南京林学院林业机械系

本文对外圆磨床砂轮架结构进行了振动分析,比较了实模态和复模态的分析情况。结果发现,对于少自由度系统来说,两者相差不多。由于实模态分析比复模态简单得多,因此对于少自由度系统,用实模态分析已可。

关键词：实模态分析法复模态分析法

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盾壳霉对油菜长效专用配方肥料的敏感性评价

中国生物防治学报 2020年第3期36卷 465-471页

作者：刘小凡谢甲涛付艳苹姜道宏程家森华中农业大学植物科学与技术学院/微生物学国家重点实验室/湖北省作物病害监测和安全控制重点实验室武汉430070

油菜菌核病是油菜生产中的重要病害,盾壳霉是核盘菌的重寄生真菌,在菌核病防治方面具有重要的生防潜力。为了明确盾壳霉与油菜长效专用配方肥料混合施用的可行性,本文研究了盾壳霉对油菜长效专用配方肥的敏感性。结果发现,低浓度的油菜... 详细信息

油菜菌核病是油菜生产中的重要病害,盾壳霉是核盘菌的重寄生真菌,在菌核病防治方面具有重要的生防潜力。为了明确盾壳霉与油菜长效专用配方肥料混合施用的可行性,本文研究了盾壳霉对油菜长效专用配方肥的敏感性。结果发现,低浓度的油菜长效专用配方肥(1.5和7.5 mg/mL)对盾壳霉菌丝生长、菌落及菌丝尖端形态和分生孢子萌发等无明显影响,高浓度的油菜长效专用配方肥对盾壳霉生长和孢子萌发有一定影响,在饱和浓度(560 mg/mL)条件下的油菜长效专用配方肥,24 h盾壳霉孢子的萌发率为0.83%,然而在96 h时萌发率可达到95%;油菜长效专用配方肥对盾壳霉产孢和寄生致腐菌核的能力无明显影响,寄生菌核30 d后,致腐指数均在50以上。研究结果表明,在田间生产中,盾壳霉可以与油菜长效专用配方肥料混合施用,达到轻简化栽培的目的。

关键词：盾壳霉核盘菌油菜菌核病油菜长效专用配方肥料敏感性

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盾壳霉对2种除草剂的敏感性评价

华中农业大学学报 2018年第5期37卷 25-31页

作者：强翠翠程家森谢甲涛付艳苹姜道宏华中农业大学农业微生物学国家重点实验室武汉430070 华中农业大学湖北省作物病害监测和安全控制重点实验室武汉430070

为了与播前除草措施相配合,达到油菜轻简化栽培的目的,开展了盾壳霉对田间常用灭生型除草剂草甘膦和草铵膦敏感性的研究。结果发现,草甘膦和草铵膦原药对盾壳霉菌丝生长有较强抑制作用,EC50分别为714.3μg/mL和574.5μg/mL;草甘膦对盾... 详细信息

为了与播前除草措施相配合,达到油菜轻简化栽培的目的,开展了盾壳霉对田间常用灭生型除草剂草甘膦和草铵膦敏感性的研究。结果发现,草甘膦和草铵膦原药对盾壳霉菌丝生长有较强抑制作用,EC50分别为714.3μg/mL和574.5μg/mL;草甘膦对盾壳霉孢子萌发抑制作用较弱,EC50为1 019.8μg/mL,而草铵膦原药对孢子的萌发基本没有抑制作用;2种除草剂原药均抑制核盘菌菌丝生长,EC50分别为1 695.8μg/mL和223.5μg/mL;2种除草剂商品制剂对盾壳霉孢子没有杀灭作用,但延迟孢子的萌发,影响盾壳霉对核盘菌菌核的寄生。研究结果表明,在田间生产中盾壳霉可以与草甘膦和草铵膦2种除草剂进行混合施用。

关键词：盾壳霉核盘菌草甘膦草铵膦敏感性