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Phosphorus-doped NiS_(2)electrocatalyst with a hybrid structure for hydrogen evolution

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Science China(Technological Sciences) 2022年第7期65卷 1506-1512页

作者： zhou Qi LIU HaoRui JIAO SunZhi FENG chenchen State Key Laboratory of Advanced Processing and Recycling of Non-ferrous Metals Lanzhou University of TechnologyLanzhou 730050China School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou 730050China College of Mechanical Engineering Longdong UniversityQingyang 745000China

In this article,P-NiS_(2)/Ni_(x)P electrocatalysts were synthesized by dealloying the Ni-Al precursor followed by phosphosulfide treatment.We found that the hybrid structure of P-NiS_(2)/Ni_(x)P provides rich active sites for the surface hydrogen evolution reaction (HER),and the doping of P enhances the electronic conductivity of electrodes.In particular,the obtained electrode shows a low overpotential of 196 mV at 10 mA cm^(-2)and a small Tafel slope of only 110 mV dec^(-1)in the HER.Meanwhile,longterm constant current electrolysis test experiments indicate that P-NiS_(2)/Ni_(x)P has good service stability.This research will help to open a new window on the design and fabrication of HER electrocatalysts.

关键词： nickle phosphosulfides hybrid structure hydrogen evolution reaction dealloying

DNA N^6-methyladenine demethylase ALKBH1 enhances osteogenic differentiation of human MSCs

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Bone Research 2016年第3期4卷 174-182页

作者： Chenchen Zhou Yuting Liu Xiaobing Li Jing Zou Shujuan Zou State Key Laboratory of Oral Diseases West China Hospital of StomatologySichuan University

ALKBH1 was recently discovered as a demethylase for DNA N6-methyladenine （N6-mA）, a new epigenetic modification, and interacts with the core transcriptional pluripotency network of embryonic stem cells. However, the role of ALKBH1 and DNA N6-mA in regulating osteogenic differentiation is largely unknown. In this study, we demonstrated that the expression of ALKBH1 in human mesenchymal stem cells （MSCs） was upregulated during osteogenic induction. Knockdown of ALKBH1 increased the genomic DNA N6-mA levels and significantly reduced the expression of osteogenic-related genes, alkaline phosphatase activity, and mineralization. ALKBHl-depleted MSCs also exhibited a restricted capacity for bone formation in vivo. By contrast, the ectopic overexpression of ALKBH1 enhanced osteoblastic differentiation. Mechanically, we found that the depletion of ALKBH1 resulted in the accumulation of N6-mA on the promoter region of ATF4, which subsequently silenced ATF4 transcription. In addition, restoring the expression of ATP by adenovirus-mediated transduction successfully rescued osteogenic differentiation. Taken together, our results demonstrate that ALKBH1 is indispensable for the osteogenic differentiation of MSCs and indicate that DNA N6-mA modifications area new mechanism for the epigenetic regulation of stem cell differentiation.

关键词： ATF Figure MSCs DNA N~6-methyladenine demethylase ALKBH1 enhances osteogenic differentiation of human MSCs

Hydrogel platform with tunable stiffness based on magnetic nanoparticles cross-linked GelMA for cartilage regeneration and its intrinsic biomechanism

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生物活性材料(英文) 2023年第7期25卷 615-628页

作者： Chenchen Zhou Chunli Wang Kang Xu Zhixing Niu Shujuan Zou Demao Zhang Zhiyong Qian Jinfeng Liao Jing Xie State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu610041China State Key Laboratory of Biotherapy State Key Laboratory and Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengdu610041SichuanChina "111"Project Laboratory of Biomechanics and Tissue Repair Bioengineering CollegeChongqing UniversityChongqing400044China College of Pharmacy Hubei University of Chinese MedicineWuhan430065China Department of Cardiology and Endodontics West China Hospital of StomatologySichuan UniversityChengduSichuan610064China

Cartilage injury affects numerous individuals,but the efficient repair of damaged cartilage is still a problem in clinic.Hydrogel is a potent scaffold candidate for tissue regeneration,but it remains a big challenge to improve its mechanical property and figure out the interaction of chondrocytes and stiffness.Herein,a novel hybrid hydrogel with tunable stiffness was fabricated based on methacrylated gelatin(GelMA)and iron oxide nanoparticles(Fe_(2)O_(3))through chemical bonding.The stiffness of Fe_(2)O_(3)/GelMA hybrid hydrogel was controlled by adjusting the concentration of magnetic nanoparticles.The hydrogel platform with tunable stiffness modulated its cellular properties including cell morphology,microfilaments and Young’s modulus of chondrocytes.Interestingly,Fe_(2)O_(3)/GelMA hybrid hydrogel promoted oxidative phosphorylation of mitochondria and facilitated catabolism of lipids in chondrocytes.As a result,more ATP and metabolic materials generated for cellular physiological activities and organelle component replacements in hybrid hydrogel group compared to pure GelMA hydrogel.Furthermore,implantation of Fe_(2)O_(3)/GelMA hybrid hydrogel in the cartilage defect rat model verified its remodeling potential.This study provides a deep understanding of the bio-mechanism of Fe_(2)O_(3)/GelMA hybrid hydrogel interaction with chondrocytes and indicates the hydrogel platform for further application in tissue engineering.

关键词： Magnetic nanoparticles Hydrogel Chondrocyte Cartilage defect Cellular metabolism

METTL5 regulates cranial suture fusion via Wnt signaling

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Fundamental Research 2023年第3期3卷 369-376页

作者： Kexin Lei Ruoshi Xu Qian Wang Qiuchan Xiong Xinyi zhou Qiwen Li Dutmanee Seriwatanachai Shuibin Lin Chenchen Zhou Quan Yuan State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China Department of Oral Biology Faculty of DentistryMahidol UniversityBangkok 73170Thailand Center for Translational Medicine Precision Medicine InstituteThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China

METTL5 is a methyltransferase that mediates eukaryotic 18S ribosomal RNA m^(6)A modification,and its mutations lead to intellectual disability,microcephaly,and facial dysmorphism in patients.However,the role of METTL5 in craniofacial development remains poorly understood.This study demonstrates that Mettl5 knockout mice exhibit poor ossification,widened cranial sutures,and a cleidocranial dysplasia-like phenotype.Deletion of Mettl5 leads to increased proliferation and decreased osteogenic differentiation of suture mesenchymal stem cells.Mechanistically,we find that Wnt signaling is significantly downregulated after Mettl5 knockout.Overall,we reveal an essential role of METTL5 in craniofacial development and osteogenic differentiation of suture mesenchymal stem cells,making METTL5 a potential diagnostic and therapeutic target for craniofacial developmental diseases.

关键词： METTL5 N6-methyladenosine Cranial suture Osteogenic differentiation Mesenchymal stem cells

circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

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International Journal of Oral Science 2022年第3期14卷 360-370页

作者： Liyuan Yu Kai Xia Jing zhou Zhiai Hu Xing Yin Chenchen Zhou Shujuan Zou Jun Liu State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Orthodontics West China Hospital of StomatologySichuan UniversityChengduChina Department of Stomatology Kunming Yan’an hospitalKunmingChina State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengduChina

Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects.

关键词： regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis miR

Microenvironmental stiffness mediates cytoskeleton re-organization in chondrocytes through laminin-FAK mechanotransduction

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International Journal of Oral Science 2022年第2期14卷 182-191页

作者： Chenchen Zhou Mengmeng Duan Daimo Guo Xinmei Du Demao Zhang Jing Xie State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengduChina Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityChengduChina

Microenvironmental biophysical factors play a fundamental role in controlling cell behaviors including cell morphology,proliferation,adhesion and differentiation,and even determining the cell fate.Cells are able to actively sense the surrounding mechanical microenvironment and change their cellular morphology to adapt to it.Although cell morphological changes have been considered to be the first and most important step in the interaction between cells and their mechanical microenvironment,their regulatory network is not completely clear.In the current study,we generated silicon-based elastomer polydimethylsiloxane(PDMS)substrates with stiff(15:1,PDMS elastomer vs.curing agent)and soft(45:1)stiffnesses,which showed the Young’s moduli of~450 k Pa and 46 kPa,respectively,and elucidated a new path in cytoskeleton re-organization in chondrocytes in response to changed substrate stiffnesses by characterizing the axis shift from the secreted extracellular protein lamininβ1,focal adhesion complex protein FAK to microfilament bundling.We first showed the cellular cytoskeleton changes in chondrocytes by characterizing the cell spreading area and cellular synapses.We then found the changes of secreted extracellular linkage protein,lamininβ1,and focal adhesion complex protein,FAK,in chondrocytes in response to different substrate stiffnesses.These two proteins were shown to be directly interacted by Co-IP and colocalization.We next showed that impact of FAK on the cytoskeleton organization by showing the changes of microfilament bundles and found the potential intermediate regulators.Taking together,this modulation axis of lamininβ1-FAK-microfilament could enlarge our understanding about the interdependence among mechanosensing,mechanotransduction,and cytoskeleton re-organization.

关键词： laminin mec stiffness

AFF4 regulates osteogenic differentiation of human dental follicle cells

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International Journal of Oral Science 2020年第4期12卷 287-297页

作者： Qingyue Xiao Yuning Zhang Xingying Qi Yaqian Chen Rui Sheng Ruoshi Xu Quan Yuan Chenchen Zhou State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontology West China Hospital of StomatologySichuan UniversityChengduChina State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Oral Implantology West China Hospital of StomatologySichuan UniversityChengduChina State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengduChina

As a member of the AFF(AF4/FMR2)family,AFF4 is a transcription elongation factor that is a component of the super elongation complex.AFF4 serves as a scaffolding protein that connects transcription factors and promotes gene transcription through elongation and chromatin remodelling.Here,we investigated the effect of AFF4 on human dental follicle cells(DFCs)in osteogenic differentiation.In this study,we found that small interfering RNA-mediated depletion of AFF4 resulted in decreased alkaline phosphatase(ALP)activity and impaired mineralization.In addition,the expression of osteogenic-related genes(DLX5,SP7,RUNX2 and BGLAP)was significantly downregulated.In contrast,lentivirus-mediated overexpression of AFF4 significantly enhanced the osteogenic potential of human DFCs.Mechanistically,we found that both the mRNA and protein levels of ALKBH1,a critical regulator of epigenetics,changed in accordance with AFF4 expression levels.Overexpression of ALKBH1 in AFF4-depleted DFCs partially rescued the impairment of osteogenic differentiation.Our data indicated that AFF4 promoted the osteogenic differentiation of DFCs by upregulating the transcription of ALKBH1.

关键词： impaired impairment alkaline

High-availability in-memory key-value store using RDMA and Optane DCPMM

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Frontiers of Computer Science 2023年第1期17卷 221-223页

作者： Xuecheng QI Huiqi HU Jinwei GUO chenchen HUANG Xuan zhou Ning XU Yu FU Aoying zhou School of Data Science and Engineering East China Normal UniversityShanghai 200062China China Construction Bank Financial Technology Co.Ltd. Shanghai 200120China

1 Introduction and main contributiions Emerging hardwares like remote Direct Memory Access(RDMA)capable networks and persistent memory(PM)are promising to build fast high availability in-memory key-value stores.The recent advent of Intel Optane DC Persistent Memory Modules(Optane DCPMM)brings the future closer.However,existing studies to combine the two devices cannot deliver the desired performance due to their two-phase protocols for log shipping and most of them were based on emulation that perform sub-optimally on real PM hardware.

关键词： hardware RDMA value

Phosphorus-doped NiS;electrocatalyst with a hybrid structure for hydrogen evolution

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Science China Technological Sciences 2022年

作者： zhou Qi LIU HaoRui JIAO SunZhi FENG chenchen State Key Laboratory of Advanced Processing and Recycling of Non-ferrous Metals Lanzhou University of Technology School of Materials Science and Engineering Lanzhou University of Technology College of Mechanical Engineering Longdong University

In this article, P-NiS;/Ni;P electrocatalysts were synthesized by dealloying the Ni-Al precursor followed by phosphosulfide treatment. We found that the hybrid structure of P-NiS;/Ni;P provides rich active sites for the surface hydrogen evolution reaction（HER）, and the doping of P enhances the electronic conductivity of electrodes. In particular, the obtained electrode shows a low overpotential of 196 mVat 10 mA cm;and a small Tafel slope of only 110 mV dec;in the HER. Meanwhile, longterm constant current electrolysis test experiments indicate that P-NiS;/Ni;P has good service stability. This research will help to open a new window on the design and fabrication of HER electrocatalysts.

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