Hydrogel platform with tunable stiffness based on magnetic nanoparticles cross-linked GelMA for cartilage regeneration and its intrinsic biomechanism

作     者：Chenchen Zhou Chunli Wang Kang Xu Zhixing Niu Shujuan Zou Demao Zhang Zhiyong Qian Jinfeng Liao Jing Xie 

作者机构：State Key Laboratory of Oral DiseasesNational Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu610041China State Key Laboratory of BiotherapyState Key Laboratory and Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengdu610041SichuanChina "111"Project Laboratory of Biomechanics and Tissue RepairBioengineering CollegeChongqing UniversityChongqing400044China College of PharmacyHubei University of Chinese MedicineWuhan430065China Department of Cardiology and EndodonticsWest China Hospital of StomatologySichuan UniversityChengduSichuan610064China 

出 版 物：《生物活性材料(英文)》 (Bioactive Materials)

年 卷 期：2023年第25卷第7期

页      面：615-628页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：This work was supported by the National Natural Science Foundation of China(81771047 and 22CXRC0216 to Jing Xie,32171354 to Jingfeng Liao,81901040 to Chenchen Zhou) China Postdoctoral Science Foundation(2019M653440) Sichuan Science and Technology Innovation Talent Project(2022JDRC0044) Chengdu International Science and Technology Cooperation Project(2020-GH02-00048-HZ). 

主　　题：Magnetic nanoparticles Hydrogel Chondrocyte Cartilage defect Cellular metabolism 

摘      要：Cartilage injury affects numerous individuals,but the efficient repair of damaged cartilage is still a problem in clinic.Hydrogel is a potent scaffold candidate for tissue regeneration,but it remains a big challenge to improve its mechanical property and figure out the interaction of chondrocytes and stiffness.Herein,a novel hybrid hydrogel with tunable stiffness was fabricated based on methacrylated gelatin(GelMA)and iron oxide nanoparticles(Fe_(2)O_(3))through chemical bonding.The stiffness of Fe_(2)O_(3)/GelMA hybrid hydrogel was controlled by adjusting the concentration of magnetic nanoparticles.The hydrogel platform with tunable stiffness modulated its cellular properties including cell morphology,microfilaments and Young’s modulus of chondrocytes.Interestingly,Fe_(2)O_(3)/GelMA hybrid hydrogel promoted oxidative phosphorylation of mitochondria and facilitated catabolism of lipids in chondrocytes.As a result,more ATP and metabolic materials generated for cellular physiological activities and organelle component replacements in hybrid hydrogel group compared to pure GelMA hydrogel.Furthermore,implantation of Fe_(2)O_(3)/GelMA hybrid hydrogel in the cartilage defect rat model verified its remodeling potential.This study provides a deep understanding of the bio-mechanism of Fe_(2)O_(3)/GelMA hybrid hydrogel interaction with chondrocytes and indicates the hydrogel platform for further application in tissue engineering.