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Distribution characteristics and controlling factors of typical heavy metals in Huanghe River estuary,China

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Journal of Oceanology and Limnology 2023年第1期41卷 150-165页

作者： Yuxi LU Dawei PAN Tingting YANG Chenchen Wang Institute of Oceanology Chinese Academy of SciencesQingdao 266071China CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation Shandong Key Laboratory of Coastal Environmental ProcessesResearch Center for Coastal Environment Engineering Technology of Shandong ProvinceYantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China University of Chinese Academy of Sciences Beijing 100049China College of Chemistry and Chemical Engineering Yantai UniversityYantai 264005China

The geochemical characteristics and potential controlling factors of colloidal Zn,Cd,and Pb in Huanghe(Yellow)River estuary(HRE),China were investigated.The three metals were highly variable over a range of spatiotemporal scales,comprehensively forced by various physical and biological processes.Total dissolved Zn,Cd,and Pb varied from 200.1 to 321.7,2.6 to 4.1,and 0.5 to 1.0 nmol/L,respectively.Only one near-estuarine station of Zn had contamination factor values>1,which indicate the lower contaminant levels.Five dissolved species of Zn,Cd and Pb were fractionated,namely<1 kDa,1-3 kDa,3-10 kDa,10-100 kDa,and 100 kDa-0.45μm.The<1 kDa truly dissolved phase was the main fraction of the three dissolved metals(50%-62%),while the 100-kDa-0.45-μm high molecular weight colloidal fraction was dominant in their respective colloidal phase.Territorial input and sediment acted as important sources of strong ligands and natural colloids for the HRE water system.<3-kDa Zn and Pb were susceptible to the dissolved oxygen,the behaviors of colloidal Zn and 3-10-kDa Pb were related to dissolved organic carbon(DOC).However,no significant correlation between each dissolved fraction of Cd and salinity,pH,temperature,colloidal organic carbon,and DOC was found in this study.Overall,these findings,completed by the evaluation of the dissolved species of Zn,Cd,and Pb at 10 sites over the river-sea mixing zone,provided new insights into the colloidal heterogeneity that affect metals geochemical features,migration and fate in estuaries.

关键词： colloidal heavy metal size fraction Huanghe(Yellow)River estuary geochemical feature

Large chromosomal segment deletions by CRISPR/LbCpf1-mediated multiplex gene editing in soybean

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Journal of Integrative Plant Biology 2021年第9期63卷 1620-1631页

作者： Kaixuan Duan Yuanyuan Cheng Jing Ji Chenchen Wang Yongshu Wei Yuanchao wang Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095China

The creation of new soybean varieties has been limited by genomic duplication and redundancy.Efficient multiplex gene editing and large chromosomal segment deletion through clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein(Cas)systems are promising strategies for overcoming these obstacles.CRISPR/Cpf1 is a robust tool for multiplex gene editing.However,large chromosomal excision mediated by CRISPR/Cpf1 has been reported in only a few non-plant species.Here,we report on CRISPR/LbCpf1-induced large chromosomal segment deletions in soybean using multiplex gene targeting.The CRISPR/LbCpf1 system was optimized for direct repeat and guide RNA lengths in crispr RNA(crRNA)array.The editing efficiency was evaluated using LbCpf1 driven by the CaMV35S and soybean ubiquitin promoter.The optimized system exhibited editing efficiencies of up to 91.7%.Our results showed eight gene targets could be edited simultaneously in one step when a single eight-gRNA-target crRNA array was employed,with an efficiency of up to 17.1%.We successfully employed CRISPR/LbCpf1 to produce small fragments(<1 Kb)and large chromosomal segment deletions(10 Kb-1 Mb)involving four different gene clusters in soybean.Together,these data demonstrate the power of the CRISPR/LbCpf1 platform for multiplex gene editing and chromosomal segment deletion in soybean,supporting the use of this technology in both basic research and agricultural applications.

关键词： chromosomal segment deletion CRISPR/LbCpf1 multiplex gene editing soybean

Palaeoclimate of the Paleogene Dongying Formation in Bozhong Sag of Bohai Bay Basin

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Petroleum Research 2021年第4期6卷 368-378页

作者： Juan Zhang Chenchen Wang Jingmin wang Linbo wang Yunqian Jia Rongrong Hu College of Earth Sciences Yangtze UniversityWuhan430100HubeiChina Laboratory of Reservoir Microstructure Evolution and Digital Characterization Wuhan430100HubeiChina Cooperative Innovation Center of Unconventional Oil and Gas Yangtze UniversityWuhan430100China School of Petroleum Engineering Yangtze UniversityWuhan430100China

It was showed that the sedimentary environment of lacustrine basins is more susceptible to basin size than that of marine basins,leading to frequent changes in sedimentary environment and large differences in sediment supply and types.Based on the comprehensive analysis of clay minerals,spore pollen,major elements and trace elements,the sedimentary environment of Dongying Formation and its evolution in Bozhong Sag were studied.The results showed that during the sedimentary period of Dongying Formation,the study area was in a subtropical warm and humid climate as a whole.In addition,the vertical distribution of spore pollen and algae was divided into four zones and each one represents different sedimentary environments.Combination zone I is characterized by a large number of spore pollen and algae,including juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,taxodiaceaepollenites hiatus,leiosphaeridia and granodiscus,and it indicates a relatively hot and dry climatic;Combination zone II mainly contains juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,pediastrum and leiosphaeridia.Compared with I,the number of spore pollen and algae decreases,but the number of pediastrum increases a lot,which represents warm and humid climate.The combination zone III is dominated by abietineaepollenites and pinuspollenites and chaetosphaeridium,and the species and quantity decrease significantly.It indicates a relatively hot and humid climate at that time.Combination zone IV contains a small amount of spore pollen and algae,and it means the climate was warm and humid at that period.The frequent changes of sedimentary environment in lacustrine basins are influenceded by regional tectonic environment and basin scale.Using S/A and E-T parameters,the paleoclimate in the study area was delineated and 10 sedimentary environment interfaces were identified,which further illustrated the characteristics of frequent fluctuations of sedimentary environment in lacustrine bas

关键词： Clastic rocks Spore pollen assemblage zone Sedimentary environment Bozhong sag Lacustrine basin

Study on sedimentation distribution based on cooperative analysis of geologic-geophysical in less well area: A case study of Suweiyi sandstone in the DB area, Kuqa Depression in Tarim Basin

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Petroleum Research 2020年第4期5卷 339-346页

作者： Huiling Niu Lixia Liang Linbo wang Chenchen Wang Juan Zhang Exploration and Development Research Intstitute Sinopec Northwest Oifield Company Urumqi 830011Xingfiang China College of Earth Sciences Yangtze University Wuhan430100HubeiChina Departoment of Earth Science and Engineering Imperial College London LondonSW72AZ UK

The Kuqa Depression in Tarim Basin develops fan deltaic,braided river deltaic,and lacustrine sedi-mentation,espeially extensive braided river deltaic sedimentation,in the Cretaceous-Paleogene Sys-tems.However,fine sedimentary pattern of the Kuqa Depression and North Tarim Uplift belt since the Middle Cenozoic still needs to be studied.As a combination zone of the Kuqa Depression and North Tarim Uplift,the sedimentary characteristics of the DB area in the southern margin of the east of the Kuqa depression have been paid more attention.To better understand the sedimentary framework in the Kuqa Depression and North Tarim Uplift in the Mesozoic and Cenozoic,through joint geologic-geophysical study,sedime ntation of sandstone at the bottom of the Paleogene Suweiyi Formation in the DB area and its peripheral area is investigated.Sedimentary facies and sandstone distribution in the area are identified through core observation,component analysis,logging interpretation and seismic inversion.Based on seismic facies analysis,sedimentary facies distribution in the area is delineated.Results show that sandstone at the bottom of the Paleogene Suweiyi Fommation in the DB area and its peripheral area is developed in shore shallow lacustrine beach-bar facies.The beach-bar arenaceous sediments are mainly distnibuted in the southern DB area and the shallow lacustrine mudstone is developed in northern DB area,showing obvious north south di ferentiation charactenistics.

关键词： Suweiyi formation Sandstone Seismic inversion Seismic facies Logging facies Sedimentary facies Kuqa depression Tarim basin

Improving the value of molecular testing:current status and opportunities in colorectal cancer precision medicine

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Cancer Biology & Medicine 2024年第1期21卷 21-28页

作者： Haiyun Li Linwei Guo Chenchen Wang Xin Hu Ye Xu Department of Colorectal Surgery Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Department of Gastrointestinal Medical Oncology Fudan University Shanghai Cancer CenterShanghai 200032China Precision Cancer Medical Center Affiliated with Fudan University Shanghai Cancer Center Shanghai 201315China

Colorectal cancer(CRC)is the second leading cause of cancer-related deaths worldwide1.Surgical radical resection with adjuvant chemotherapy remains the primary treatment choice for CRC,but the 5-year postoperative survival rate is only approximately 60%,and approximately one-third of patients with CRC experience recurrence within 2 years of surgery2.Fortunately,the transformation of high-throughput sequencing has accelerated the development of precision medicine.For example,KRAS mutations indicate resistance to anti-epidermal growth factor receptor(EGFR)-targeted therapies in CRC3.Furthermore,molecular-guided individualized therapy has brought new promise in major clinical areas and challenges,such as novel biomarkers predicting sensitivity and resistance to immunotherapy for microsatellite stable(MSS)CRC.

关键词： cancer chemotherapy colorectal

Ultralow-strain Ti substituted Mn-vacancy layered oxides with enhanced stability for sodium-ion batteries

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Journal of Energy Chemistry 2021年第12期30卷 351-357,I0008页

作者： Yanchen Liu Chenchen Wang Meng Ren Hengyi Fang Zhuoliang Jiang Fujun Li Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Renewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin 300071China

Anionic redox reaction(ARR) in layered manganese-based oxide cathodes has been considered as an effective strategy to improve the energy density of sodium-ion batteries.Mn-vacancy layered oxides deliver a high ARR-related capacity with small voltage hysteresis,however,they are limited by rapid capacity degradation and poor rate capability,which arise from inferior structure changes due to repeated redox of lattice oxygen.Herein,redox-inactive Ti^(4+)is introduced to substitute partial Mn^(4+)to form Na_(2) Ti_(0.5)Mn_(2.5)O_7(Na_(4/7)[□_(1/7)Ti_(1/7)Mn_(5/7)]O_(2),□ for Mn vacancies),which can effectively restrain unfavorable interlayer gliding of Na2 Mn307 at high charge voltages,as reflected by an ultralow-strain volume variation of 0.11%.There is no irreversible O_(2) evolution observed in Na_(2) Ti_(0.5)Mn_(2.5)O_7 upon charging,which stabilizes the lattice oxygen and ensures the overall structural stability.It exhibits increased capacity retention of 79.1% after 60 cycles in Na_(2) Ti_(0.5)Mn_(2.5)O_7(17.1% in Na_(2) Mn_(3) O_7) and good rate capability(92.1 mAh g^(-1) at 0.5 A g^(-1)).This investigation provides new insights into designing high-performance cathode materials with reversible ARR and structural stability for SIBs.

关键词： Mn vacancy Layered oxide cathode Anionic redox reaction Sodium-ion batteries

A novel PD-L1 targeting peptide self-assembled nanofibers for sensitive tumor imaging and photothermal immunotherapy in vivo

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Nano Research 2022年第8期15卷 7286-7294页

作者： Linping Fu Jianhu Zhang chenchen Wu Weizhi wang Dong wang Zhiyuan Hu Zihua wang CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Key Laboratory of Standardization and Measurement for NanotechnologyCAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China School of Nanoscience and Technology Sino-Danish CollegeUniversity of Chinese Academy of SciencesBeijing 100049China Fujian Provincial Key Laboratory of Brain Aging and Neurodegenerative Diseases School of Basic Medical SciencesFujian Medical UniversityFuzhou 350122China School of Chemical Engineering and Pharmacy Wuhan Institute of TechnologyWuhan 430205China School of Chemistry and Chemical Engineering Beijing Institute of TechnologyBeijing 100081China Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhen 518060China

Programmed death 1(PD-1)and its ligand PD-L1 are two typical immune checkpoints.Antibody-based immune checkpoint blockade(ICB)strategy targeting PD-1/PD-L1 achieved a significant therapeutic effect on cancer.However,due to the impenetrability of antibody drugs and the occurrence of immune-related adverse events,only a minority of patients benefit from this treatment.Peptides multimerization has been widely proved to be an effective method to improve receptor binding affinity through a multivalent synergistic effect.In this study,we report a novel peptide-aggregation-induced emission(AIE)hybrid supramolecular TAP,which can self-assemble into nanofibers through non-covalent interactions such as hydrogen bonds,with a specific nanomolar affinity to PD-L1 in vivo and in vitro.Combined with near-infrared agents,it can be used for tumor imaging and photothermal therapy,which enables photothermal ablation of cancer cells for generating tumor-associated antigen(TAA)and triggering a series of immunological events.Collectively,our work suggests that synthetic self-assembled peptide nanofibers can be developed as attractive platforms for active photothermal immunotherapies against cancer.

关键词： programmed death 1(PD-1) targeting peptide self-assembly immune checkpoint blockade photothermal therapy

An integrated portable bio-monitoring system based on tough hydrogels for comprehensive detection of physiological activities

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Nano Research 2024年第1期17卷 321-332页

作者： Congcong Yang chenchen Ji Fengjiao Guo Chunjiang Jin Hongyu Mi Zhongchang wang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources School of Chemical Engineering and TechnologyXinjiang UniversityUrumqi 830017China International Iberian Nanotechnology Laboratory(INL) Avenide Mestre Jose VeigaBraga 4715-330Portugal

Advanced soft ion-conducting hydrogels have been developed rapidly in the integrated portable health monitoring equipment due to their higher sensitivity,sensory traits,tunable conductivity,and stretchability for physiological activities and personal healthcare detection.However,traditional hydrogel conductors are normally susceptible to large deformation and strong mechanical stress,which leads to inferior electro-mechanical stability for real application scenarios.Herein,a strong ionically conductive hydrogel(poly(vinyl alcohol)-boric acid-glycerol/sodium alginate-calcium chloride/electrolyte ions(PBG/SC/EI))was designed by engineering the covalently and ionically crosslinked networks followed by the salting-out effect to further enhance the mechanical strength and ionic conductivity of the hydrogel.Owing to the collective effects of the energy-dissipation mechanism and salting-out effect,the designed PBG/SC/EI with excellent structural integrity and robustness exhibits exceptional mechanical properties(elongation at break for 559.1%and tensile strength of 869.4 kPa)and high ionic conductivity(1.618 S·m^(-1)).As such,the PBG/SC/EI strain sensor features high sensitivity(gauge factor=2.29),which can effectively monitor various kinds of human motions(joint motions,facial micro-expression,faint respiration,and voice recognition).Meanwhile,the hydrogel-based Zn||MnO_(2)battery delivers a high capacity of 267.2 mAh·g^(-1)and a maximal energy density of 356.8 Wh·kg^(-1)associated with good cycle performance of 71.8%capacity retention after 8000 cycles.Additionally,an integrated bio-monitoring system with the sensor and Zn||MnO_(2)battery can accurately identify diverse physiological activities in a real-time and non-invasive way.This work presents a feasible strategy for designing high-performance conductive hydrogels for highly-reliable integrated bio-monitoring systems with excellent practicability.

关键词： Ionically conductive hydrogel strain sensor Zn-based battery integrated bio-monitoring system human motion monitoring

The role and applications of extracellular vesicles in osteoporosis

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Bone Research 2024年第1期12卷 62-77页

作者： Fei Fang Jie Yang Jiahe wang Tiantian Li Erxiang wang Demao Zhang Xiaoheng Liu chenchen Zhou Institute of Biomedical Engineering West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu 610041China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China

Osteoporosis is a widely observed condition characterized by the systemic deterioration of bone mass and microarchitecture,which increases patient susceptibility to fragile fractures.The intricate mechanisms governing bone homeostasis are substantially impacted by extracellular vesicles(EVs),which play crucial roles in both pathological and physiological contexts.EVs derived from various sources exert distinct effects on osteoporosis.Specifically,EVs released by osteoblasts,endothelial cells,myocytes,and mesenchymal stem cells contribute to bone formation due to their unique cargo of proteins,miRNAs,and cytokines.Conversely,EVs secreted by osteoclasts and immune cells promote bone resorption and inhibit bone formation.Furthermore,the use of EVs as therapeutic modalities or biomaterials for diagnosing and managing osteoporosis is promising.Here,we review the current understanding of the impact of EVs on bone homeostasis,including the classification and biogenesis of EVs and the intricate regulatory mechanisms of EVs in osteoporosis.Furthermore,we present an overview of the latest research progress on diagnosing and treating osteoporosis by using EVs.Finally,we discuss the challenges and prospects of translational research on the use of EVs in osteoporosis.

关键词： osteoporosis homeostasis biomaterials