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Oxidation behavior of Ni-based superalloy GH738 in static air between 800 and 1000℃

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Rare Metals 2021年第3期40卷 616-625页

作者： Jue Wang hao xue Ying Wang Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology Nanjing 211167China School of Materials Science and Engineering Nanjing Institute of TechnologyNanjing 211167China

The oxidation behavior of a nickel-based superalloy GH738 was studied by isothermal oxidation tests in still air at different temperatures,with exposure time up to 100 ***-kinetic curves were plotted using the mass gain *** surface and cross-sectional morphologies were observed by scanning electron microscopy(SEM).A composition analysis and an oxidation-product identification were conducted using energydispersive X-ray spectroscopy(EDS) and an X-ray diffraction(XRD),*** results showed that GH738 exhibited parabolic oxidation-kinetic curves,with stable parabolic-rate constants at each *** activation energy of oxide growth was calculated to be329.6 kJ·mol^(-1).Cr_(2) O_(3)(chromia) was the external oxidation product at 800℃.A TiO_(2)-Cr_(2) O_(3) double-layer structure was formed at 900℃.The position of TiO_(2) changed from the oxide-metal interface to the air-oxide interface by the diffusion of Ti atoms in chromia during the oxidation time at this *** was observed in the Cr_(0.12)Ti_(0.78)O_(1.74)-Cr_(2) O_(3) multi-layer oxide of at 1000℃,which increased the oxidation *** all the tests,the main internal oxide was always Al_(2) O_(3).The entire GH738 oxidation process was interpreted by the competitive diffusion of elements(Cr,Ti,Ni,etc.) in metal matrix and chromia,while a schematic diagram of oxidation process was proposed.

关键词： GH738 Oxidation behavior Diffusion Multilayer structure

Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus

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Journal of Integrative Agriculture 2017年第1期16卷 115-124页

作者： chen Zhe ZHANG Ming-hao ZHOU xue-ping WU Jian-xiang State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang University State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

Aphid-borne Zucchini yellow mosaic virus （ZYMV） is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection technique. Using purified ZYMV virion and the conventional hybridoma technology, three hybridoma cell lines （16A11, 5A7 and 3B8） secreting monoclonal antibodies （MAbs） against ZYMV Zhejiang isolate were obtained. The working titers of the ascitic fluids secreted by the three hybridoma cell lines were up to 10^-7 by indirect enzyme-linked immunosorbent assay （ELISA）. All MAbs were isotyped as IgG1, kappa light chain. Western blot analysis indicated that the MAb 3B8 could specifically react with the coat protein of ZYMV while MAbs 5A7 and 16A11 reacted strongly with a protein of approximately 51 kDa from the ZYMV-infected leaf tissues. According to this molecular weight, we consider this reactive protein As likely to be the HC-Pro protein. Using these three MAbs, we have now developed five detection assays, i.e., antigen-coated-plate ELISA （ACP-ELISA）, dot-ELISA, tissue blot-ELISA, double-antibody sandwich ELISA （DAS-ELISA）, and immunocapture-RT-PCR （IC-RT-PCR）, for the sensitive, specific, and easy detection of ZYMV. The sensitivity test revealed that ZYMV could be readily detected respectively by ACP-ELISA, dot-ELISA, DAS-ELISA and IC-RT-PCR in 1：163840, 1：2560, 1：327680 and 1：1 310720 （w/v, g mL-1） diluted crude extracts from the ZYMV-infected plants. We demonstrated in this study that the dot-ELISA could also be used to detect ZYMV in individual viruliferous aphids. A total of 275 cucurbitaceous plant samples collected from the Zhejiang, Jiangsu, Shandong and Hainan provinces, China, were screened for the presence of ZYMV with the described assays. Our results showed that 163 of the 275 samples （59%） were infected with ZYMV. This finding indicates that ZYMV As now widely present in cucurbitaceous crops in China. RT-PCR followed by DNA sequencing and sequence a

关键词： Zucchini yellow mosaic virus monoclonal antibody ACP-ELISA dot-ELISA tissue blot-ELISA DAS-ELISA IC-RT-PCR

Entanglement of Two-Qubit Quantum Heisenberg XYZ Chain

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Chinese Physics Letters 2002年第8期19卷 1044-1047页

作者： Xi Xiao-Qiang hao San-Ru chen Wen-xue Yue Rui-Hong Institute of Modern Physics Northwest University Xi'an 710069 Fundamental Department of Xi'an Institute of Posts and Telecommunications Xi'an 710061

We derive the analytic expression of the concurrence in the quantum Heisenberg XYZ model and discuss the influence of parameters J, Δ and Γ on the concurrence. By choosing different values of Γ and Δ, we obtain the XX, XY, XXX and XXZ chains. The concurrence decreases with increasing temperature. When T→0, the concurrence reaches its maximum value 1, *** entangled state, |Ψ = (((2)1/2)/2)(|01-|10), is maximum entanglement. For the XXZ chain, when Γ→∞, the concurrence will meet its maximum value max = (sinh (1/T))/(cosh (1/T)).

关键词： Chains

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我国蔬菜作物耐低温性研究进展

中国蔬菜 2019年第6期 29-34页

作者：钱恒彦潘宝贵刁卫平戈伟郭广君刘金兵陈学好王述彬扬州大学园艺与植物保护学院江苏扬州225100 江苏省农业科学院蔬菜研究所江苏省高效园艺作物遗传改良重点实验室江苏南京210014

低温胁迫是蔬菜生产过程中的主要逆境因子,对蔬菜作物的生长发育、商品产量形成影响极大。本文综述了蔬菜作物耐低温性的遗传模型、生理调节和分子机制,展望了蔬菜作物耐低温性的研究前景。

关键词：蔬菜低温胁迫生理调节分子机制综述

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《种子检验学》教学模式的探索与实践

实验科学与技术 2011年第6期9卷 137-138页

作者：谢皓陈学珍南张杰赵波北京农学院植物科学技术学院北京102206

《种子检验学》是农学专业的核心课。为强化实验教学,对《种子检验学》教学内容、教学方法和考评方式等方面进行改革。教学实践表明,这些改革措施对于调动学生学习的主动性,提高《种子检验学》的教学质量起到了良好的引导和促进作用。

关键词：种子检验学教学实践

A New Zosterophyll Plant,Ramoferis ***.,from the Posongchong Formation of Lower Devonian (Pragian) of Southeastern Yunnan,China

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Acta Geologica Sinica(English Edition) 2011年第4期85卷 765-776页

作者： hao Shougang xue Jinzhuang Key Laboratory of Orogenic Belts and Crustal Evolution School of Earth and Space SciencesPeking UniversityBeijing 100871China

A new genus and species,Ramoferis amalia *** ***.,is described from the Early Devonian（Pragian）Posongchong Formation,Wenshan district of southeastern Yunnan,*** plant has isodichotomous major axes,which also divide anisotomously distally to produce closely spaced lateral branches,often within fertile *** stalked round to reniform sporangia,borne laterally and spirally over several levels of branches,are distantly to moderately spaced or aggregated into distinct *** stalks increase in width from the base to the sporangial junction resulting in a gradual transition between sporangium and *** with known zosterophylls shows that this plant is most similar to the genera Zosterophyllum and Wenshania,differing in the character combinations of sporangial shape,sporangium/stalk junction,stalk dimensions and orientation,and especially in both sterile and fertile branchlets being borne within the fertile *** addition,the observation and comparison indicate distinct variety in structure of sporangial border and suggest that the two-layered sporangium wall characterized by outer anticlinal layer and inner periclinal layer might represent potential synapomorphies,while the different dehiscence mechanism is an autapomorphy for each taxon in zosterophylls.

关键词： zosterophyll Early Devonian Posongchong Flora Yunnan

Multifaceted functions of Drp1 in hypoxia/ischemia- induced mitochondrial quality imbalance: from regulatory mechanism to targeted therapeutic strategy

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Military Medical Research 2024年第4期11卷 589-615页

作者： Shuai hao He Huang Rui-Yan Ma xue Zeng chen-Yang Duan Department of Anesthesiology the Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China Research Institute of General Surgery Jinling HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210002China Department of Cardiovascular Surgery Xinqiao HospitalArmy Medical UniversityChongqing 400037China Institute for Brain Science and Disease Chongqing Medical UniversityChongqing 400010China

Hypoxic-ischemic injury is a common pathological dysfunction in clinical *** are sensitive organelles that are readily damaged following ischemia and ***-related protein 1(Drp1)regulates mitochondrial quality and cellular functions via its oligomeric changes and multiple modifications,which plays a role in mediating the induction of multiple organ damage during hypoxic-ischemic ***,there is active controversy and gaps in knowledge regarding the modification,protein interaction,and functions of Drp1,which both hinder and promote development of Drp1 as a novel therapeutic ***,we summarize recent findings on the oligomeric changes,modification types,and protein interactions of Drp1 in various hypoxic-ischemic diseases,as well as the Drp1-mediated regulation of mitochondrial quality and cell functions following ischemia and ***,potential clinical translation prospects for targeting Drp1 are *** review provides new ideas and targets for proactive interventions on multiple organ damage induced by various hypoxic-ischemic diseases.

关键词： Dynamin-related protein 1(Drp1) Hypoxic-ischemic injury Mitochondrial quality imbalance Cell dysfunction Organ damage

Effect of Sargassum fusiforme polysaccharide on apoptosis and its possible mechanism in human erythroleukemia cells

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Chinese Journal of Natural Medicines 2020年第10期18卷 749-759页

作者： DING hao-Miao chen xue-Jia chen Hai-Min WANG Cai-Sheng QIAN Guo-Ying School of Marine Sciences Ningbo UniversityNingbo 315211China College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingbo 315100China

This study aimed to investigate the effects of Sargassum fusiforme polysaccharide(SFPS I,II,and III)on the apoptosis and regulation of human erythroleukemia(HEL)*** effect of different doses of SFPS on HEL cell growth was detected using the Cell Counting Kit-8 method,and apoptosis was detected by Hoechst *** cycle distribution and apoptosis were detected using flow *** of the cell cycle gene,p53,antiapoptotic genes,Bcl-xL and Bcl-2,and pro-apoptotic genes,Bax,Bad,and Caspase-3,as well as the expression of the corresponding proteins,were detected using real-time quantitative polymerase chain reaction(qPCR)and Western *** results showed that SFPS Ⅱ and Ⅲ decreased HEL cell viability and induced HEL cell *** concentrations of SFPS(Ⅰ,Ⅱ,and Ⅲ)were detected that induced much less toxic effect in normal human embryonic lung(MRC-5)cells,and SFPS Ⅰ increased cell proliferation,indicating its favorable selectivity towards cancer *** mechanism by which SFPS induced apoptosis was also found to be related to the induction of cell cycle arrest in the G0/G1 phase and the increased expression of apoptosis-related genes and *** concluded that SFPS induces HEL cell apoptosis,possibly via activation of the Caspase pathway,providing the theoretical basis for the development of SFPS-based anti-tumor drug products.

关键词： Sargassum fusiforme Polysaccharides Human erythroleukemia(HEL cells) Apoptosis Cell cycle

Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3287-3301页

作者： Ziwei Fan Mengxian Jia Jian Zhou Zhoule Zhu Yumin Wu Xiaowu Lin Yiming Qian Jiashu Lian Xin Hua Jianhong Dong Zheyu Fang Yuqing Liu Sibing chen Xiumin xue Juanqing Yue Minyu Zhu Ying Wang Zhihui Huang Honglin Teng Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Neurology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord *** of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be *** possesses an anti-inflammatory biological ***,its roles and the underlying molecular mechanisms in spinal cord injury remain *** this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in *** promoted M2 polarization through inactivating the cGAS/STING/NF-κB ***,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord ***,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal *** together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.

关键词： cGAS/STING functional recovery microglia neuroinflammation neuroprotection nuclear factor-κB polarization spinal cord injury tryptanthrin