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Polyphenol based hybrid nano-aggregates modified collagen fibers of biological valve leaflets to achieve enhanced mechanical,anticoagulation and anti-calcification properties

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Journal of Leather Science and Engineering 2022年第4期4卷 394-408页

作者： Shufen Li Shiying Lang Zhiqian chen jingruo chen Weihua Zhuang Yangrui Du Yawen Yao Gongyan Liu Mao chen Laboratory of Heart Valve Disease West China HospitalSichuan University37 Guoxue AlleyChengdu 610041China The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education College of Biomass Science and EngineeringSichuan UniversityChengdu 610065China University of California Davis 1 Shields AveDavisCA 95616USA Faculty of Science and Engineering Aachen-Maastricht Institute for Biobased Materials(AMIBM)Maastricht UniversityBrightlands Chemelot Campus6167 RD GeleenThe Netherlands Department of Cardiology West China HospitalSichuan University37 Guoxue AlleyChengdu 610041China Regenerative Medicine Research Center West China HospitalSichuan University37 Guoxue AlleyChengdu 610041China

Glutaraldehyde(Glut)-crosslinked porcine pericardium and bovine pericardium are mainly consisted of collagen and widely used for the preparation of heterogenous bioprosthetic heart valves(BHV),which play an important role in the replacement therapy of severe valvular heart disease,while their durability is limited by degeneration due to calcification,thrombus,endothelialization difficulty and prosthetic valve ***,we develop a novel BHV,namely,TPly-BP,based on natural tannic acid and polylysine to improve the durability of Glut crosslinked bovine pericardium(Glut-BP).Impressively,tannic acid and polylysine could form nanoaggregates via multiple hydrogen bonds and covalent bonds,and the introduction of nanoaggregates not only improved the mechanical properties and collagen stability but also endowed TPly-BP with good biocompatibility and *** to Glut-BP,TPly-BP showed significantly reduced cytotoxicity,improved endothelial cell adhesion,a low hemolysis ratio and obviously reduced platelet ***,TPly-BP exhibited great antibacterial and in vivo anti-calcification ability,which was expected to improve the in vivo durability of *** results suggested that TPly-BP would be a potential candidate for BHV.

关键词： Tannic acid Bioprosthetic heart valve Antibacterial and anti-calcification

Expression of insulin-like growth factor-1 in breast cancer tissues

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The Chinese-German Journal of Clinical Oncology 2009年第6期8卷 326-328页

作者： Mingxun chen Mengquan Li jingruo Li Department of Breast Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China

Objective: We investigated the expression of insulin-like growth factor-1 (IGF-1) so as to explore its relationship with carcinogenesis and development of breast cancer. Methods: IGF-1 mRNA levels in tissues of breast cancer, adjacent breast cancer in 70 cases breast cancer patients were analyzed by RT-PCR with the normal breast tissues of paired breast as the control. Results: The level of IGF-1 mRNA expression in breast cancer tissues was significantly higher than that in the paired adjacent to breast cancer tissues, normal mammary gland tissues. The ration of IGF-1/β-actin were 0.679 ± 0.075, 0.463 ± 0.085, 0.305 ± 0.031, respectively. There was significant difference between different groups (P 0.005). Conclusion: The high-level expression of IGF-1 in breast cancer tissues is correlated with carcinogenesis, development and metastasis of breast cancer.

关键词： insulin-like growth factor-1 (IGF-1) breast cancer

The Effects of Herceptin and its Combination with 2-Deoxyglucose on Proliferation,Migration and Invasion of ErbB2-overexpressing Breast Cancer Cells

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The Effects of Herceptin and its Combination with 2-Deoxyglu...

中国生物化学与分子生物学会2020全国学术在线会议

作者： Sinan Lv Yue Shen Li He jingruo chen Xuejiao Ma Hua Li Yuhua Zhao Department of Biochemistry and Molecular Biology West China School of Basic Medical Sciences and Forensic MedicineSichuan University Department of Anatomy West China School of Basic Medical Sciences and Forensic MedicineSichuan University Department of Key Laboratory of Drug Targeting and Drug Delivery System of Ministry of Education West China School of PharmacySichuan University

Purpose:To investigate the effects of Herceptin alone and in combination with 2-deoxyglucose(2-DG) on proliferation,migration,and invasion of ErbB2-overexpressing breast cancer cell lines MCF7/ErbB2 and SK-BR-3,as well as their underlying molecular ***:MCF7/ErbB2 and SK-BR-3 cells were treated with PBS,2-DG,Herceptin alone or in combination with *** proliferation was detected by MTS *** migration and invasion were measured by transwell *** uptake and lactate production in the media were measured by using Glucose Oxidase Assay kit and Lactate Assay kit,***2,lactate dehydrogenase A(LDHA) and hexokinase II(HKII) protein levels were detected by Western *** addition,the combination index(CI) was calculated to evaluate if there was a synergism of combination ***:In MCF7/ErbB2 and SK-BR-3 cells,Herceptin alone effectively inhibited proliferation,migration,invasion,glucose uptake and lactate production,and downregulated ErbB2 and LDHA protein *** of Herceptin with 2-DG synergistically inhibits proliferation,migration,and invasion of both *** to 2-DG or Herceptin alone group,combination group significantly inhibited glucose uptake and lactate production in both *** both cell lines,compared to PBS group,Herceptin alone decreased HKII protein level;the combination significantly inhibited ErbB2,LDHA,HKII protein *** SK-BR-3 cells,the inhibitory effect on the expression of LDHA and HKII protein by combination was more prominent than 2-DG or Herceptin individually ***,these differences were not observed in MCF7/ErbB2 ***:We concluded that combining Herceptin with 2-DG synergistically inhibited proliferation,migration,and invasion of MCF7/ErbB2 and SK-BR-3 cells by downregulating LDHA and HKII protein levels which affected cell glycolysis.

关键词： Breast neoplasms ErbB2 Glycolysis Herceptin 2-Deoxyglucose

来源： cnki会议评论

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论魏晋士人的自然主义生命意识

理论界 2022年第12期 35-42页

作者：陈静若胡静中国人民大学哲学院中央团校(中国青年政治学院)马克思主义学院

“自然”是道家哲学的重要概念。自先秦时期,老、庄即主张回归本真、顺应自然,以自然态度面对人生。至魏晋之际,玄学大畅,士人个体生命意识觉醒,其思想以先秦道家为主流,体现鲜明的自然主义色彩,由此发展出王弼的“喜、惧、哀、乐,民之... 详细信息

“自然”是道家哲学的重要概念。自先秦时期,老、庄即主张回归本真、顺应自然,以自然态度面对人生。至魏晋之际,玄学大畅,士人个体生命意识觉醒,其思想以先秦道家为主流,体现鲜明的自然主义色彩,由此发展出王弼的“喜、惧、哀、乐,民之自然”、嵇康的“越名教而任自然”,以及郭象的“物皆自然,无物使然”等观点。魏晋士人通过对“自然”的体悟,追求生命本真的存在状态,向往诗意自由的人生,彰显自然主义的生命意识,对魏晋士人群体本身、魏晋社会、美学发展乃至后世哲学都产生了深远影响。

关键词：魏晋士人自然生命意识道家

Heregulin-β1诱导硬脂酰辅酶A去饱和酶1促进乳腺癌细胞的增殖、迁移和侵袭

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第三军医大学学报 2020年第11期42卷 1091-1100页

作者：陈璟若马雪娇吕思楠安琪尔赵玉华四川大学华西基础医学与法医学院生物化学与分子生物学教研室成都610041 四川大学华西药学院微生物与生化药学教研室成都610041

目的探讨heregulin-β1(HRG-β1)诱导硬脂酰辅酶A去饱和酶1(stearoyl-CoA desaturase-1,SCD1)的表达及SCD1活性对乳腺癌细胞MCF7和SK-BR-3增殖、迁移和侵袭能力的影响。方法用PBS(对照组)或不同浓度和作用时间下HRG-β1处理MCF7和SK-BR-3细胞,采用RT-qPCR和Western blot检测细胞SCD1 mRNA和蛋白表达。MTT实验检测HRG-β1和SCD1抑制剂A939572处理后的MCF7和SK-BR-3细胞增殖能力。划痕实验和Transwell实验分别检测HRG-β1、A939572和油酸(oleic acid,OA)处理后的MCF7和SK-BR-3细胞迁移和侵袭能力。结果用HRG-β1处理MCF7和SK-BR-3细胞,两种细胞的SCD1 mRNA和蛋白表达水平均增加,且均表现出时间-剂量依赖关系;与对照组比较,HRG-β1处理后两种细胞的增殖率明显增加(P<0.01),而加入A939572后,两种细胞的增殖率明显下降(P<0.01);与对照组比较,HRG-β1处理后MCF7和SK-BR-3细胞划痕迁移率和侵袭细胞数显著增加(P<0.01)。HRG-β1与A939572联用后,划痕迁移率和侵袭细胞数明显降低(P<0.01),而回补OA后,划痕迁移率和侵袭细胞数均显著增加(P<0.01)。结论 HRG-β1能上调SCD1的表达水平,且HRG-β1对乳腺癌细胞MCF7和SK-BR-3的增殖、迁移和侵袭的促进作用部分是由SCD1介导的。

关键词： Heregulin-β1 硬脂酰辅酶A去饱和酶1 乳腺癌增殖迁移侵袭

2-脱氧葡萄糖对乳腺癌MCF7/ErbB2细胞增殖迁移和侵袭的影响

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西部医学 2018年第11期30卷 1564-1569页

作者：申越贺丽李华陈璟若徐硕赵玉华四川大学华西基础医学与法医学院人体解剖学教研室四川成都610041 四川大学华西基础医学与法医学院生物化学与分子生物学教研室四川成都610041 四川大学华西药学院微生物与生化药学教研室四川成都610041

目的探讨糖酵解抑制剂2-脱氧葡萄糖(2-Deoxy-D-glucose,2-DG)对乳腺癌MCF7/ErbB2细胞增殖、迁移和侵袭的影响及其分子机制。方法用不同浓度的2-DG(0,0.5,1,2,4,8,16,32mM)处理MCF7/ErbB2细胞48h后,用MTS试剂盒测定细胞的增殖;用Transwell实验检测不同浓度2-DG(0,0.5,1,2mM)对MCF7/ErbB2细胞的迁移和侵袭的影响;用不同浓度2-DG(0,0.5,1,2mM)处理MCF7/ErbB2细胞48h后,用葡萄糖和乳酸检测试剂盒分别测定培养基中的葡萄糖浓度和乳酸浓度,并计算细胞对葡萄糖的摄取量和乳酸生成量;用1mM 2-DG处理MCF7/ErbB2细胞0、4、8、12、24h后,用Western blot检测己糖激酶II(hexokinase II,HKII)蛋白表达水平。结果与2-DG未处理组比较,2-DG(0.5~32mM)能抑制MCF7/ErbB2细胞的增殖;2-DG(0.5,1,2mM)能显著抑制MCF7/ErbB2细胞的迁移和侵袭(P<0.001),同时显著降低葡萄糖摄取量和乳酸生成量(P<0.001),而且上述抑制作用均表现出对2-DG剂量的依赖性;1mM 2-DG显著下调MCF7/ErbB2细胞中HKII的蛋白水平。结论 2-DG通过下调HKII蛋白表达降低糖酵解水平,从而抑制乳腺癌MCF7/ErbB2细胞的增殖、迁移和侵袭。

关键词： 2-脱氧葡萄糖乳腺癌增殖迁移侵袭糖酵解

Cell polarization in ischemic stroke: molecular mechanisms and advances

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Neural Regeneration Research 2025年第3期20卷 632-645页

作者： Yuanwei Li Xiaoxiao Xu Xuan Wu Jiarui Li Shiling chen Danyang chen Gaigai Li Zhouping Tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Ischemic stroke is a cerebrovascular disease associated with high mortality and disability rates. Since the inflammation and immune response play a central role in driving ischemic damage, it becomes essential to modulate excessive inflammatory reactions to promote cell survival and facilitate tissue repair around the injury site. Various cell types are involved in the inflammatory response, including microglia, astrocytes, and neutrophils, each exhibiting distinct phenotypic profiles upon stimulation. They display either proinflammatory or anti-inflammatory states, a phenomenon known as ‘cell polarization.’ There are two cell polarization therapy strategies. The first involves inducing cells into a neuroprotective phenotype in vitro, then reintroducing them autologously. The second approach utilizes small molecular substances to directly affect cells in vivo. In this review, we elucidate the polarization dynamics of the three reactive cell populations(microglia, astrocytes, and neutrophils) in the context of ischemic stroke, and provide a comprehensive summary of the molecular mechanisms involved in their phenotypic switching. By unraveling the complexity of cell polarization, we hope to offer insights for future research on neuroinflammation and novel therapeutic strategies for ischemic stroke.

关键词： astrocyte polarization immune regulation inflammation ischemic injury microglia polarization neutrophil polarization signaling pathways stroke

小干扰RNA沉默己糖激酶2对人乳腺癌SK-BR-3细胞增殖、迁移与侵袭的影响

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华西药学杂志 2021年第1期36卷 19-22页

作者：马雪娇陈璟若吕思楠余蓉赵玉华四川大学华西药学院靶向药物及释药系统教育部重点实验室四川成都610041 四川大学华西基础医学与法医学院四川成都610041

目的在体外研究小干扰RNA(siRNA)沉默己糖激酶2(HK2)对人乳腺癌SK-BR-3细胞增殖、迁移和侵袭的影响及其分子机制。方法在SK-BR-3细胞中分别转染siRNA阴性、siHK2-A和siHK2-B,分别采用荧光定量PCR和Western Blot法检测HK2 mRNA和蛋白的... 详细信息

目的在体外研究小干扰RNA(siRNA)沉默己糖激酶2(HK2)对人乳腺癌SK-BR-3细胞增殖、迁移和侵袭的影响及其分子机制。方法在SK-BR-3细胞中分别转染siRNA阴性、siHK2-A和siHK2-B,分别采用荧光定量PCR和Western Blot法检测HK2 mRNA和蛋白的表达水平;MTT法检测细胞的增殖;Transwell小室检测细胞的迁移和侵袭;葡萄糖检测试剂盒检测细胞对葡萄糖的摄取。结果与阴性对照组比较,siHK2能显著降低SK-BR-3细胞的HK2 mRNA及蛋白水平,抑制细胞增殖、迁移和侵袭能力,减少细胞对葡萄糖的摄取。结论 siHK2可能通过降低葡萄糖摄取来抑制SK-BR-3细胞的增殖、迁移和侵袭。

关键词：乳腺癌小干扰RNA 己糖激酶2 SK-BR-3细胞增殖迁移侵袭葡萄糖摄取表皮生长因子受体2

Brain region-specific roles of brain-derived neurotrophic factor in social stress-induced depressive-like behavior

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Neural Regeneration Research 2025年第1期20卷 159-173页

作者： Man Han Deyang Zeng Wei Tan Xingxing chen Shuyuan Bai Qiong Wu Yushan chen Zhen Wei Yufei Mei Yan Zeng Brain Science and Advanced Technology Institute Wuhan University of Science and TechnologyWuhanHubei ProvinceChina Geriatric Hospital Affiliated to Wuhan University of Science and Technology WuhanHubei ProvinceChina School of Public Health Wuhan University of Science and TechnologyWuhanHubei ProvinceChina

Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral *** studies have shown that brain-derived neurotrophic factor expression in stressed mice is brain region–specific,particularly involving the corticolimbic system,including the ventral tegmental area,nucleus accumbens,prefrontal cortex,amygdala,and *** how brain-derived neurotrophic factor participates in stress processing in different brain regions will deepen our understanding of social stress *** this review,we discuss the expression and regulation of brain-derived neurotrophic factor in stress-sensitive brain regions closely related to the pathophysiology of *** focused on associated molecular pathways and neural circuits,with special attention to the brain-derived neurotrophic factor–tropomyosin receptor kinase B signaling pathway and the ventral tegmental area–nucleus accumbens dopamine *** determined that stress-induced alterations in brain-derived neurotrophic factor levels are likely related to the nature,severity,and duration of stress,especially in the above-mentioned brain regions of the corticolimbic ***,BDNF might be a biological indicator regulating stress-related processes in various brain regions.

关键词： amygdala chronic mild stress chronic social defeat stress corticolimbic system depression hippocampus medial prefrontal cortex nucleus accumbens social stress models ventral tegmental area