检索结果-南通市图书馆

Updates on CRISPR-based gene editing in HIV-1/AIDS therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2022年第1期37卷 1-10页

作者： Zhihao Zhang Wei hou Shuliang chen Institute of Medical Virology School of Basic Medical SciencesWuhan UniversityWuhan430071China

Although tremendous efforts have been made to prevent and treat HIV-1 infection,HIV-1/AIDS remains a major threat to global human health.The combination antiretroviral therapy(cART),although able to suppress HIV-1 replication,cannot eliminate the proviral DNA integrated into the human genome and thus requires lifelong treatment that may lead to various side effects.In recent years,clustered regularly interspaced short palindromic repeat(CRISPR)-associated nuclease 9(Cas9)related gene-editing systems have been developed and designed as effective ways to treat HIV-1 infection.However,new gene-targeting tools derived from or functioning like CRISPR/Cas9,including base editor,prime editing,SHERLOCK,DETECTR,PAC-MAN,ABACAS,pfAGO,have been developed and optimized for pathogens detection and diseases correction.Here,we summarize recent studies on HIV-1/AIDS gene therapy and provide more gene-editing targets based on studies relating to the molecular mechanism of HIV-1 infection.We also identify the strategies and potential applications of these new gene-editing technologies for HIV-1/AIDS treatment in the future.Moreover,we discuss the caveats and problems that should be addressed before the clinical use of these versatile CRISPR-based gene targeting tools.Finally,we offer alternative solutions to improve the practice of gene targeting in HIV-1/AIDS gene therapy.

关键词： HIV-1/AIDS CRISPR/Cas9 Gene editing Gene therapy

Experimental and numerical simulation on the sliding process of roof rock blocks triggered by dynamic disturbance

在线全文

学校读者我要写书评

暂无评论

Underground Space 2023年第3期10卷 269-293页

作者： Feng Dai Wancheng Zhu Min Ren Leilei Niu Chen Hou Center for Rock Instability and Seismicity Research Department of Mining EngineeringSchool of Resources and Civil EngineeringNortheastern UniversityShenyang 110819China Faculty of Land Resources Engineering Kunming University of Science and TechnologyKunming 650093China

With the excavation of underground opening,the roof rock block may fall under external dynamic disturbance.In this paper,the roof rock mass was simplified as a rock block system composed of trapezoidal rock blocks.A series of experiments and numerical simulations were performed to study the sliding process of the key block under the horizontal static clamping load and vertical impact disturbance.The propagation of stress wave in the block system were captured and analyzed by using high-speed camera and digital image correlation technique.The results reveal that a pendulum-type wave was generated due to the propagation and superposition of stress waves in the block system.Therefore,the governing mechanism of the sliding displacement of the key block was clarified based on the propagation of incident stress waves or pendulum-type waves.Meanwhile,it is found that the sliding distance of the key block decreases in a power function with the increasing friction coefficient,or decreases in a parabolic function with the increasing trapezoid internal angle.Finally,a case study on the roof block sliding of the roadway at a gold mine was conducted,and it is concluded that the sliding of the key block resulted from the coupled effects of"shear driving"and"low friction"driven by stress wave propagation,regardless of a single or multi-layer rock block system.These results may provide technical guideline for preventing rock-falling accidents caused by blasting distur-bances in underground mining.

关键词： Roof rock block Sliding instability Dynamic disturbance Pendulum-type wave Digital image correlation technique Numerical simulation

A novel and powerful approach for designing future crops—target editing promoter overcomes tradeoffs caused by gene pleiotropy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第10期65卷 2128-2130页

作者： Qingqing hou Jing Wang Xuewei chen State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute Sichuan Agricultural University at WenjiangChengdu 61l130China

Over the past decades,crop breeding has undergone genetic improvement through semi-dwarfness,heterosis,and ideal plant architecture,which increases yield tremendously to feed the growing population worldwide.However,crop breeding is still largely constrained by tradeoffs among different agronomic traits,such as negative correlation between seed size and number.

关键词： breeding crops dwarf

Gut microbes regulate the feeding center:a newdiscovery of Gut Brain Axis

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第9期7卷 3168-3169页

作者： Fengwu chen Kajian hou Zhe-Sheng chen Department of Endocrinology and metbolic diseases The First Afliated Hospital of Shantou University Medical Collge515000 ShantouGuangdongPR China Department of Pharmaceutical Sciences College of Pharmacy and Health SciencesSt.John's UniversityNew YorkNY 11439USA

Recently,llana Gabanyi and colleagues published an article in Science,1identifying a microbial sensing mechanism that regulates feeding behavior and host metabolism.In their study,a sex-and age-dependent gut-brain cross-talk is revealed.Their study confrmed that hypothalamic neurons can directly sense the structural components of the gut bacterial microbiota and regulate feeding and nesting behavior,affecting food intake and body temperature.

关键词： temperature. intake feeding

Heat Transfer Characteristics of a Microchannel Heat Sink with Highly-Dense Micro-Jet Arrays

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Thermal Science 2024年第3期33卷 1148-1160页

作者： ZHANG Yu chen Xiaoyan MIAO Lin chen Liang hou Yu State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong UniversityXi’an710049China Beijing Aerospace Technology Institute Beijing100083China

This paper proposed a new structure of highly-dense micro-jet arrays for hybrid jet-impingement/microchannel heat sinks(~120 jet per cm^(2) with jet width of 0.35 mm).Parametric study is performed to investigate the influence of structure and flow parameters on the convective heat transfer of water jet cooling in confined space.The simulation results show that the optimal jet width,jet spacing and impingement distance are around 0.2 mm,0.2 mm and 0.3 mm,respectively,which can achieve a low thermal resistance as well as a relatively low pressure drop.The analysis of the heat transfer pathways shows that the micro-fin jet structures can extend the impinging heat transfer area and conduct a considerable proportion of heat,which can reach up to 38.9%with the Reynolds number ranging from 797.1 to 5602.2.The heat transfer characteristics in the heat sink will shift from impingement dominated heat transfer to the channel-convection dominated heat transfer as the jet impingement distance increases.A correlation is proposed to predict the average Nusselt number on the stagnation area for the heat sink with different structure parameters,and the deviations of predictions from the numerical results are less than 10%.

关键词： hybrid heat sink impingement microchannel thermal management high heat flux

Pomegranate peel polyphenols alleviate insulin resistance through the promotion of insulin signaling pathway in skeletal muscle of metabolic syndrome rats

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2022年第4期11卷 1076-1085页

作者： Xitong Zhang Lin Du Weimin Zhang Mi Yang Li chen Chen Hou Jianke Li College of Food Engineering and Nutritional Science Shaanxi Normal UniversityXi’an 710119China University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization Xi’an 710119China

Insulin resistance(IR) has been considered to be an important causative factor of metabolic syndrome(Met S). The present study investigated whether pomegranate peel polyphenols(PPPs) could prevent the development of Met S by improving IR in rats. Male Sprague-Dawley(SD) rats were fed high fat diet(HFD) to induce Met S and supplemented with different dosages of PPPs for 12 weeks. The results showed that HFD-induced insulin resistant rats had disordered metabolism of blood glucose, blood lipid, and terrible muscle fiber morphology when compared with normal diet-fed rats, but PPPs treatment at a dosage of 300 mg/kg·day significantly reversed these negative effects. Moreover, in skeletal muscle tissue of insulin resistant rats, PPPs treatments significantly increased the protein expressions of insulin receptor(Ins R) and phosphorylated insulin receptor substrate 1(IRS-1), stimulated peroxisome proliferator activated receptor gamma(PPARγ) and phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT/PKB) signaling pathway, and aggrandized the protein levels of phosphorylated glycogen synthase kinase-3β(GSK-3β) and glucose transporter 4(GLUT4). Our results suggest that PPPs possess of the beneficial effects on alleviating IR by enhancing insulin sensitivity and regulating glucose metabolism.

关键词： Metabolic syndrome Insulin resistance Insulin signaling pathway PPARγ Pomegranate peel polyphenols

Crack Fault Diagnosis and Location Method for a Dual-Disk Hollow Shaft Rotor System Based on the Radial Basis Function Network and Pattern Recognition Neural Network

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2023年第2期36卷 180-197页

作者： Yuhong Jin Lei hou Zhenyong Lu Yushu chen School of Astronautics Harbin Institute of TechnologyHarbin 150001China Institute of Dynamics and Control Science Shandong Normal UniversityJi’nan 250014China

The crack fault is one of the most common faults in the rotor system,and researchers have paid close attention to its fault diagnosis.However,most studies focus on discussing the dynamic response characteristics caused by the crack rather than estimating the crack depth and position based on the obtained vibration signals.In this paper,a novel crack fault diagnosis and location method for a dual-disk hollow shaft rotor system based on the Radial basis function(RBF)network and Pattern recognition neural network(PRNN)is presented.Firstly,a rotor system model with a breathing crack suitable for a short-thick hollow shaft rotor is established based on the finite element method,where the crack's periodic opening and closing pattern and different degrees of crack depth are considered.Then,the dynamic response is obtained by the harmonic balance method.By adjusting the crack parameters,the dynamic characteristics related to the crack depth and position are analyzed through the amplitude-frequency responses and waterfall plots.The analysis results show that the first critical speed,first subcritical speed,first critical speed amplitude,and super-harmonic resonance peak at the first subcritical speed can be utilized for the crack fault diagnosis.Based on this,the RBF network and PRNN are adopted to determine the depth and approximate location of the crack respectively by taking the above dynamic characteristics as input.Test results show that the proposed method has high fault diagnosis accuracy.This research proposes a crack detection method adequate for the hollow shaft rotor system,where the crack depth and position are both unknown.

关键词： Hollow shaft rotor Breathing crack Radial basis function network Pattern recognition neural network Machine learning

Self-deposition for mesoporous carbon nanosheet with supercapacitor application

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2023年第3期55卷 34-40页

作者： Juan Du Aibing chen Senlin hou Xueqing Gao College of Chemical and Pharmaceutical Engineering Hebei University of Science and TechnologyShijiazhuang 050018China The Second Hospital of Hebei Medical University Shijiazhuang 050000China

Porous carbon sheets have wide application prospects in many fields,especially in energy storage of supercapacitor due to the features combining both 2D structure and porous architectures.Herein,a self-deposition approach is proposed to obtain N-doped mesoporous carbon nanosheets (N-MCNs),using 3-aminophenol (3-AF) as precursor and Mg(OH)_(2) sheet as hard template.This process realizes the direct carbon formation using 3-AF monomer as carbon precursor under the catalysis of hard template avoiding the polymerization and utilization of solvent.The mass ratio of 3-AF to Mg(OH)_(2) plays an important role in determining the pore structures and the resulting capacitance behavior.The results show that N-MCNs with a mass ratio of 3-AF and Mg(OH)_(2) of 1:1 have good electrochemical behavior for supercapacitors.This N-MCNs based electrode exhibits a high capacitance of 240 F·g^(-1)at 1 A·g^(-1),good rate performance(75.4%retention ratio at 20 A·g^(-1)),and high cycling stability with 98.3% initial capacitance retained after 10000 cycles.Symmetric supercapacitors on N-MCNs achieve energy density of 18.2 W·h·kg^(-1) and power density of 0.4 kW·kg^(-1) operated within a wide potential range of 0–1.6 V in 1.0 mol·L^(-1) Na_(2)SO_(4) solution,exhibiting its potential for electrode materials with high performance.

关键词： Monomer self-deposition Mg(OH)_(2)catalysis Hard template Carbon nanosheet Supercapacitor

Dichloroacetic acid and rapamycin synergistically inhibit tumor progression

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第5期24卷 397-405页

作者： Huan chen Kunming LIANG Cong hou Hai-long PIAO CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Department of Biochemistry&Molecular Biology School of Life SciencesChina Medical UniversityShenyang 110122China University of Chinese Academy of Sciences Beijing 100049China

Mammalian target of rapamycin(mTOR)controls cellular anabolism,and mTOR signaling is hyperactive in most cancer cells.As a result,inhibition of mTOR signaling benefits cancer patients.Rapamycin is a US Food and Drug Administration(FDA)-approved drug,a specific mTOR complex 1(mTORC1)inhibitor,for the treatment of several different types of cancer.However,rapamycin is reported to inhibit cancer growth rather than induce apoptosis.Pyruvate dehydrogenase complex(PDHc)is the gatekeeper for mitochondrial pyruvate oxidation.PDHc inactivation has been observed in a number of cancer cells,and this alteration protects cancer cells from senescence and nicotinamide adenine dinucleotide(NAD^(+))exhaustion.In this paper,we describe our finding that rapamycin treatment promotes pyruvate dehydrogenase E1 subunit alpha 1(PDHA1)phosphorylation and leads to PDHc inactivation dependent on mTOR signaling inhibition in cells.This inactivation reduces the sensitivity of cancer cells'response to rapamycin.As a result,rebooting PDHc activity with dichloroacetic acid(DCA),a pyruvate dehydrogenase kinase(PDK)inhibitor,promotes cancer cells'susceptibility to rapamycin treatment in vitro and in vivo.

关键词： Dichloroacetic acid(DCA) Rapamycin Pyruvate dehydrogenase E1 subunit alpha 1(PDHA1) Mammalian target of rapamycin(mTOR)