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Science China(Life Sciences) 2018年第12期61卷 1463-1464页

作者： chen, hualan CAAS Harbin Vet Res Inst Harbin Heilongjiang Peoples R China Natl Avian Influenza Reference Lab Harbin Heilongjiang Peoples R China

*** chen is a virologist whose research focuses on understanding influenza virus and developing vaccines against this *** studied veterinary medicine as her undergraduate and graduate majors in Gansu Agricultural University,receiving her Bachelor degree and Master degree in 1991 and 1994,*** 1997,she received her Ph. *** the Graduate School of the Chinese Academy of Agricultural Sciences(CAAS).

关键词： the Chinese Academy veterinary medicine

Inactivated H9N2 vaccines developed with early strains do not protect against recent H9N2 viruses:Call for a change in H9N2 control policy

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Journal of Integrative Agriculture 2024年第6期23卷 2144-2148页

作者： Yanjing Liu Qingqing Yu Xiangyu Zhou Wenxin Li Xinwen He Yan Wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng hualan chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070China State Key Laboratory for Animal Disease Control and Prevention Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry DiseasesMinistry of Agriculture and Rural Affairs/Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China National Poultry Laboratory Animal Resource Center Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China Harbin Weike Biotechnology Co. Ltd.Harbin 150009China

H9N2 virus has been widely distributed in wild birds and poultry around the world since its first emergence in the United States of America in 1966(Gu et al.2017;Carnaccini and Perez 2020).The virus appeared in chickens in China in the early 1990s,and over the last two decades has gradually become the dominant epidemic subtype(Sun and Liu 2015;Bi et al.2020).Although H9N2 virus infection alone cannot cause severe disease or death in poultry,H9N2 virus-infected birds experience a degree of egg production drop and can be easily infected by other pathogens,thus causing economic losses for poultry industry.

关键词： H9N2 poultry policy

M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

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Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen Hu Li Jiang Guangwen Wang Yangming Song Zhibo Shan Xuyuan Wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng Liling Liu hualan chen chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

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A型流感病毒:新型内化受体的发现

中国兽医科学 2024年第8期54卷 1135-1135页

作者： Zixin Ni Jinliang Wang Xiaofei Yu Yifan Wang Jingfei Wang Xijun He chengjun Li Guohua Deng Jianzhong Shi Huihui Kong Yongping Jiang Pucheng chen Xianying Zeng Guobin Tian hualan chen Zhigao Bu State Key Laboratory for Animal Disease Control Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin People’s Republic of China

A型流感病毒(IAV)已发生多次大流行,造成了严重的危害。仅1918年的西班牙流感就造成了约5000万人的死亡,近年来,H7和H5亚型高致病性流感的爆发更造成了全球范围内逾1000例患者以及约4.2亿只家禽的死亡。IAV为分节段的单股负链RNA病毒,... 详细信息

A型流感病毒(IAV)已发生多次大流行,造成了严重的危害。仅1918年的西班牙流感就造成了约5000万人的死亡,近年来,H7和H5亚型高致病性流感的爆发更造成了全球范围内逾1000例患者以及约4.2亿只家禽的死亡。IAV为分节段的单股负链RNA病毒,病毒颗粒呈球形或丝状,直径约为80~120 nm。病毒由囊膜、基质蛋白及核心组成。其核心为结合病毒单股负链RNA的RNA聚合酶复合物,基质蛋白构成了病毒的外壳骨架,囊膜包含两种重要的糖蛋白,即血凝素(HA)和神经氨酸酶(NA)。NA的作用主要是协助释放病毒颗粒促其黏附于呼吸道上皮细胞,HA结合细胞受体,在病毒侵入细胞的过程中起着重要的作用。

关键词： A型流感病毒病毒颗粒基质蛋白细胞受体负链RNA病毒 H5亚型呼吸道上皮细胞高致病性

Vaccination of poultry successfully eliminated human infection with H7N9 virus in China

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Science China(Life Sciences) 2018年第12期61卷 1465-1473页

作者： Xianying Zeng Guobin Tian Jianzhong Shi Guohua Deng chengjun Li hualan chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

The H7 N9 viruses that emerged in China in 2013 were nonpathogenic in chickens but mutated to a highly pathogenic form in early 2017 and caused severe disease outbreaks in chickens. The H7 N9 influenza viruses have caused five waves of human infection, with almost half of the total number of human cases(766 of 1,567) being reported in the fifth wave, raising concerns that even more human infections could occur in the sixth wave. In September 2017, an H5/H7 bivalent inactivated vaccine for chickens was introduced, and the H7 N9 virus isolation rate in poultry dropped by 93.3% after vaccination. More importantly,only three H7 N9 human cases were reported between October 1, 2017 and September 30, 2018, indicating that vaccination of poultry successfully eliminated human infection with H7 N9 virus. These facts emphasize that active control of animal disease is extremely important for zoonosis control and human health protection.

关键词： H7N9 influenza virus evolution vaccination human infection elimination

团队协作式照护策略联合舒适护理对巨大子宫肌瘤术后患者的影响

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中国医学创新 2024年第25期21卷 103-107页

作者：邬白红陈花兰王楠邹满花南昌市第一医院妇产科江西南昌330008

目的:探讨团队协作式照护策略联合舒适护理对巨大子宫肌瘤术后患者的影响。方法:选取2023年4月—2024年4月南昌市第一医院收治的80例巨大子宫肌瘤患者,随机分为两组,各40例,对照组行常规护理,观察组行团队协作式照护策略联合舒适护理。... 详细信息

目的:探讨团队协作式照护策略联合舒适护理对巨大子宫肌瘤术后患者的影响。方法:选取2023年4月—2024年4月南昌市第一医院收治的80例巨大子宫肌瘤患者,随机分为两组,各40例,对照组行常规护理,观察组行团队协作式照护策略联合舒适护理。评估两组术后康复指标、疾病认知水平、心理状态、自我效能、自护能力。结果:观察组住院时间、下床活动时间、术后清醒时间、进食时间均优于对照组,差异均有统计学意义(P<0.05)。干预后,两组疾病认知水平、一般自我效能感量表(GSES)、自我护理能力测定量表(ESCA)各项评分均高于干预前,焦虑自评量表(SAS)及抑郁自评量表(SDS)评分均低于干预前,且观察组疾病认知水平、GSES评分均高于对照组,SDS、SAS评分均低于对照组,差异均有统计学意义(P<0.05)。结论:团队协作式照护策略联合舒适护理可缓解子宫肌瘤患者术后情绪状态,加强患者的疾病知识水平,提升自护能力,改善患者的自我效能,有利于促进身体康复。

关键词：团队协作式照护策略舒适护理巨大子宫肌瘤患者术后情绪状态

2019-2022年中国H6N1亚型禽流感病毒的生物学特性分析

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中国农业科学 2024年第9期57卷 1820-1832页

作者：陈源崔鹏飞施建忠张元成于晴晴颜成张亚萍王丛丛张洁王燕邓国华陈化兰中国农业科学院哈尔滨兽医研究所/动物疫病防控全国重点实验室/国家禽类实验动物资源库

【背景】H6亚型禽流感病毒（avian influenza virus,AIV）广泛流行于我国南方地区，是我国家禽中最常见AIV之一。H6N1亚型AIV频繁地与其他野鸟源毒株重配，并且可以作为供体为高致病性AIV提供内部基因片段，产生新型重组病毒，进而威胁... 详细信息

【背景】H6亚型禽流感病毒（avian influenza virus,AIV）广泛流行于我国南方地区，是我国家禽中最常见AIV之一。H6N1亚型AIV频繁地与其他野鸟源毒株重配，并且可以作为供体为高致病性AIV提供内部基因片段，产生新型重组病毒，进而威胁人类健康。【目的】通过对我国H6N1亚型AIV的演化动态及其相关生物学特性分析研究，为我国禽流感的综合防控提供数据支持。【方法】采集2019—2022年我国25个省（直辖市、自治区）的活禽交易市场及养殖场家禽喉和泄殖腔拭子，通过接种鸡胚分离到7株H6N1亚型AIVs并对其进行全基因组测序，分析其遗传演化特征、受体结合特性及其对SPF鸡和BALB/c小鼠的感染性。【结果】遗传演化分析表明，7株病毒的基因与分离于北美及东南亚地区的野鸟源病毒基因同源性较高，基因来源复杂，具有明显的遗传多样性。贝叶斯演化分析表明，H6亚型AIV HA基因曾发生过多次的跨洲际传播，欧亚谱系病毒在北美地区也有着较长时间流行。1株病毒HA基因与北美地区毒株基因高度同源，根据贝叶斯演化分析结果，推测该病毒在野鸟体内经历了复杂的基因重配后形成，后经野鸟传入我国。特殊氨基酸位点分析结果显示，病毒HA蛋白裂解位点序列均为PQIETR↓GLF，符合低致病性AIV特征；此外，另有1株病毒的NP蛋白发生Y52H突变，据报道，该突变对AIV获得抵抗人干扰素刺激基因BTN3A3的能力起到关键作用。受体结合特性分析表明，部分毒株具有双受体结合特性，但结合人源受体能力弱于结合禽源受体能力。病毒对SPF鸡的感染性试验表明，鸡感染A/chicken/Jiangxi/S40445/2019(H6N1)后能通过呼吸道及消化道排毒，并且病毒可在鸡群内通过接触传播。鸡感染A/duck/Jiangxi/S10941/2019(H6N1)后仅有少数鸡通过呼吸道排毒，病毒无法在鸡群间通过接触传播。BALB/c小鼠的感染性试验表明，H6N1亚型AIV无需提前适应便可在小鼠呼吸道内有效复制，但对小鼠仍呈低致病力。【结论】2019—2022年分离于我国的H6N1亚型AIV基因大部分来源于野鸟源病毒，候鸟可经东亚-澳大利亚迁徙路线将病毒传入我国；部分病毒能够结合人源唾液酸受体并在小鼠呼吸道内有效复制，表明该亚型病毒对公共卫生安全构成潜在威胁。

关键词： H6N1 禽流感病毒跨洲际传播重配感染性

Glycosylation and an amino acid insertion in the head of hemagglutinin independently affect the antigenic properties of H5N1 avian influenza viruses

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Science China(Life Sciences) 2019年第1期62卷 76-83页

作者： Chunyang Gu Xianying Zeng Yangming Song Yanbing Li Liling Liu Yoshihiro Kawaoka Dongming Zhao hualan chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Division of Virology Department of Microbiology and Immunology Institute of Medical Science University of Tokyo

Antigenic drift forces us to frequently update influenza vaccines; however, the genetic basis for antigenic variation remains largely unknown. In this study, we used clade 7.2 H5 viruses as models to explore the molecular determinants of influenza virus antigenic variation. We generated eight monoclonal antibodies(MAbs) targeted to the hemagglutinin(HA) protein of the index virus A/chicken/Shanxi/2/2006 and found that two representative antigenically drifted clade 7.2 viruses did not react with six of the eight MAbs. The E131 N mutation and insertion of leucine at position 134 in the HA protein of the antigenically drifted strains eliminated the reactivity of the virus with the MAbs. We also found that the amino acid N131 in the H5 HA protein is glycosylated. Our results provide experimental evidence that glycosylation and an amino acid insertion or deletion in HA influence antigenic variation.

关键词： Influenza virus H5N1 antigenic variation genetic basis

Continued evolution of the Eurasian avian-like H1N1 swine influenza viruses in China

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Science China(Life Sciences) 2023年第2期66卷 269-282页

作者： Fei Meng Yan chen Zuchen Song Qiu Zhong Yijie Zhang Chuanling Qiao cheng Yan Huihui Kong Liling Liu chengjun Li Huanliang Yang hualan chen State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Animal influenza viruses continue to pose a threat to human public health. The Eurasian avian-like H1N1(EA H1N1) viruses are widespread in pigs throughout Europe and China and have caused human infections in several countries, indicating their pandemic potential. To carefully monitor the evolution of the EA H1N1 viruses in nature, we collected nasal swabs from 103,110pigs in 22 provinces in China between October 2013 and December 2019, and isolated 855 EA H1N1 viruses. Genomic analysis of 319 representative viruses revealed that these EA H1N1 viruses formed eight different genotypes through reassortment with viruses of other lineages circulating in humans and pigs, and two of these genotypes(G4 and G5) were widely distributed in *** studies indicated that some strains have become highly pathogenic in mice and highly transmissible in ferrets via respiratory droplets. Moreover, two-thirds of the EA H1N1 viruses reacted poorly with ferret serum antibodies induced by the currently used H1N1 human influenza vaccine, suggesting that existing immunity may not prevent the transmission of the EA H1N1 viruses in humans. Our study reveals the evolution and pandemic potential of EA H1N1 viruses and provides important insights for future pandemic preparedness.

关键词： H1N1 influenza immunity