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Mesenchymal stromal cell-derived small extracellular vesicles modulate macrophage polarization and enhance angio-osteogenesis to promote bone healing

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Genes & Diseases 2022年第4期9卷 841-844页

作者： Shang Jiunn Chuah chee Weng Yong Kristeen Ye Wen Teo Jacob Ren Jie Chew Yi Ann Cheow Shipin Zhang Raymond chung Wen wong Asher Ah Tong Lim Sai Kiang Lim Wei Seong Toh Faculty of Dentistry National University of Singapore119085Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Department of Biomedical Engineering Faculty of EngineeringNational University of Singapore117583Singapore Tissue Engineering Program Life Sciences InstituteNational University of Singapore117456Singapore Integrative Sciences and Engineering Program NUS Graduate SchoolNational University of Singapore119077Singapore

Mesenchymal stem/stromal cells(MSCs)have demonstrated therapeutic efficacy for bone regeneration in animal and clinical *** MSCs were initially thought to differentiate to various cell types to replace the injured/diseased tissue,it is now accepted that these cells secrete factors to promote tissue repair.1 Among these factors,small extracellular vesicles(sEVs)of size 50–200 nm,which include the exosomes,have been identified as the principal agent mediating the wide-ranging therapeutic efficacy of MSCs.2 Several studies have also reported the therapeutic effects of MSC-sEVs to enhance bone repair in animal models,as recently reviewed.3 However,the cellular processes and mechanisms mediated by MSC-sEVs in bone regeneration remain to be fully elucidated.

关键词： healing clinical macrophage

Surface Patterning of Self-healing P(MMA/nBA)Copolymer for Dynamic Control Cell Behaviors

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Chinese Journal of Polymer Science 2020年第7期38卷 696-703,I0005页

作者： Su-Su Liu Ze-Hong Xiang Zhi-Fang Ma Xue-Wen WU Qiang Shi Shing-chung wong Jing-Hua Yin School of Chemical Engineering Xiangtan UniversityXiangtan 411105China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Department of Mechanical Engineering University of AkronOhio 44323-3903USA

Cell behaviors are regulated by a dynamic and complex environment characterized by biophysical,mechanical and biochemical ***,most works regulate cell behaviors under static conditions or by external *** control cell adhesion and proliferation with a dynamic and mechanical environment,we pattern the surface on self-healing copolymer P(MMA/nBA).The copolymer P(MMA/nBA)with the composition of 48/52(MMA/nBA)recovers nearly 100%of its original tensile strains after 86 h of recovery from *** physical patterns on P(MMA/nBA)film are obtained over large areas and the size of the hole and the width of connecting bar are in line with the copper grid *** patterned surface tends to be fiat after 12 h with almost 75%-80%*** with cell incubation on polystyrene fiat and patterned surface of P(MMA/nBA)film without self-healing capability,the number and morphology of cells are well manipulated on the patterned surface of self-healing P(MMA/nBA)*** approach provides a convenient method for dynamically regulating the cell behaviors on the surface of self-healing materials without chemical or biological modifications.

关键词： Surface pattern Self-healing Cell behavior Dynamic

Indocyanine green lymphangiography-guided liposuction in breast cancer-related lymphedema treatment-patient selection and technique

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Plastic and Aesthetic Research 2020年第2期7卷 30-36页

作者： Hin-Lun Liu Melody Man-Kuen wong Joseph Hon-Ping chung Division of Plastic and Reconstructive Surgery Department of SurgeryThe University of Hong KongQueen Mary HospitalHong Kong SARChina

Aim:The rationale of using liposuction to treat lymphedema is that the chronic inflammatory process of lymphedema results in fat *** authors describe the technique of indocyanine green lymphangiography-guided liposuction,its rationale,and our patient selection criteria for better clinical ***:Thirty-two patients underwent liposuction for breast cancer-related *** green lymphangiography was performed prior to *** patients without linear and splash patterns in indocyanine green lymphangiography,circumferential liposuction was performed *** patients who had linear or splash patterns,liposuction was not performed at regions with remaining functional lymphatic *** were assessed using circumferential reduction ***:At a mean follow-up of 24.5±6.5 months,all(100%)patients had a reduction in limb circumferences after *** mean circumference reduction rate was 67.6%±27.9%.Conclusion:Liposuction is a valuable treatment for breast cancer-related *** believe patients with fat predominant lymphedema are the best candidates for liposuction.

关键词： Lymphedema breast cancer-related lymphedema liposuction indocyanine green lymphangiography

Chemical Analyses of Palm Kernel Oil-Based Polyurethane Prepolymer

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Materials Sciences and Applications 2012年第2期3卷 78-86页

作者： chee Sien wong Khairiah Haji Badri 不详

Polyurethane (PU) was prepared from palm kernel oil-based monoester polyol (PKO-p) via prepolymerization method at NCO/OH ratio of 200/100, 150/100, 100/100, and 75/100 at ambient temperature under nitrogen gas atmosphere. The structure of the synthesized prepolymerized PKO-p PU was determined using FTIR and 13C NMR. The disapperance of NCO peak in the FTIR spectrum at 2270 cm–1 - 2250 cm–1 cm showed that MDI has completely reacted to form PU. The appearance of C=O peak at 1700 cm–1 indicated that hydrogen bonding was formed between the soft segmented chain of the PKO-p and the hard segmented MDI. Hence, urethane bond was the main polymeric chain in the PU.

关键词： Palm Kernel Oil Prepolymerization Polyurethane Soft Segment Hard Segment

HepG2 cells recovered from apoptosis show altered drug responses and invasiveness

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Hepatobiliary & Pancreatic Diseases International 2014年第3期13卷 293-300页

作者： Shan-Shan Wang Xin Xie chung Sing Timothy wong Pui Ying Choi Ming Chiu Fung School of Life Sciences the Chinese University of Hong Kong Department of Anatomical and Cellular Pathology Prince of Wales Hospital the Chinese University of Hong Kong

BACKGROUND: Cancer relapse, associated with increased drug resistance and rate of metastasis, often follows completion of chemotherapy but the cancer escape mechanisms are still incompletely understood. Percutaneous ethanol injection(PEI)has been used for treating hepatocellular carcinoma(HCC)for decades, while the recurrence after PEI treatment remains a major limitation. Recent evidence mounted that cancer cells could survive from chemical induced apoptosis, suggesting a potential route through which cancer relapse may occur. This study focuses on the consequence of HepG2 recovery from ethanol-induced apoptotic ***: The model of HepG2 recovery from ethanolinduced apoptotic event was established by live cell imaging,BrdU assay and Western blotting. MTT assay, wound healing assay and invasion assay were used to investigate the behavior of HepG2 after ***: HepG2 cells could recover from ethanol-induced apoptosis. These cells changed their behaviors such as drug resistance, mobility and invasiveness. On average, the recovered HepG2 cell clones were found to be 46% more resistant to ethanol and 84% higher in mobility. The recovered clones became 58.2% more sensitive to ***: HepG2 cells can recover from ethanolinduced apoptotic event. These cells became more resistant to ethanol and more invasive. Although the recovered cell clones were more resistant to ethanol, they became more sensitive to5-fluorouracil treatment.

关键词： cancer recurrence apoptosis recovery liver cancer percutaneous ethanol injection 5-fluorouracil

Outcomes of right-lobe and left-lobe living-donor liver transplantations using small-for-size grafts

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World Journal of Gastroenterology 2017年第23期23卷 4270-4277页

作者： wong Hoi She Kenneth SH Chok James YY Fung Albert CY Chan chung Mau Lo Department of Surgery the University of Hong Kong Department of Surgery and State Key Laboratory for Liver Research The University of Hong Kong Department of Medicine and State Key Laboratory for Liver Research The University of Hong Kong

AIM To analyze the outcomes of living-donor liver transplantation(LDLT) using left-lobe(LL) or right-lobe(RL) small-for-size(SFS) *** Prospectively collected data of adult patients who underwent LDLT at our hospital in the period from January 2003 to December 2013 were reviewed. The patients were divided into the RL-LDLT group and the LL-LDLT group. The two groups were compared in terms of short-and long-term outcomes, including incidence of postoperative complication, graft function, graft survival, and patient survival. A SFS graft was defined as a graft with a ratio of graft weight(GW) to recipient standard liver volume(RSLV)(GW/RSLV) of < 50%. The Urata formula was used to estimate *** Totally 218 patients were included for analysis, with 199 patients in the RL-LDLT group and 19 patients in the LL-LDLT group. The two groups were similar in terms of age(median, 53 years in the RL-LDLT group and 52 years in the LL-LDLT group, P = 0.997) but had significantly different ratios of men to women(165:34 in the RL-LDLT group and 8:11 in the LL-LDLT group, P < 0.0001). The two groups were also significantly different in GW(P < 0.0001), GW/RSLV(P < 0.0001), and graft cold ischemic time(P = 0.007). When it comes to postoperative complication, the groups were comparable(P = 0.105). Five patients died in hospital,4(2%) in the RL-LDLT group and 1(5.3%) in the LLLDLT group(P = 0.918). There were 38 graft losses, 33(16.6%) in the RL-LDLT group and 5(26.3%) in the LL-LDLT group(P = 0.452). The 5-year graft survival rate was significantly better in the RL-LDLT group(95.2% vs 89.5%, P = 0.049). The two groups had similar 5-year patient survival rates(RL-LDLT: 86.8%, LL-LDLT: 89.5%, P = 0.476).CONCLUSION The use of SFS graft in LDLT requires careful tailormade surgical planning and meticulous operation. LLLDLT can be a good alternative to RL-LDLT with similar recipient outcomes but a lower donor risk. Further research into different patient conditions is needed i

关键词： Small for size liver graft Right lobe graft Left lobe graft Living donor liver transplantation

Submacular hemorrhage:treatment update and remaining challenges

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Annals of Eye Science 2017年第1期2卷 7-11页

作者： chee Wai wong Jan Carlo Yu Alegre Yew San Ian Yeo Chui Ming Gemmy Cheung Singapore Eye Research Institute Singapore National Eye CentreSingapore Ophthalmology and Visual Sciences Academic Clinical Program Duke-NUS Medical SchoolNational University of SingaporeSingapore

Submacular haemorrhage(SMH)is a sight threatening complication that can occur in exudative age related macular degeneration(AMD),but has been described to occur more frequently in eyes with polypoidal choroidal vasculopathy(PCV).Left untreated,SMH carries a grave visual ***,expedient diagnosis and effective management of this complication is of paramount *** treatment strategies for SMH include(I)displacement of blood from the fovea,usually by injection of an expansile gas;(II)pharmacologic clot lysis such as with recombinant tissue plasminogen activator(rtPA);and(III)treatment of the underlying choroidal neovascularization(CNV)or PCV,such as with anti-vascular endothelial growth factor(anti-VEGF)*** three strategies have been employed in isolation or in combination,some concurrently and others in *** has demonstrable effect on the liquefaction of submacular clots but there are remaining uncertainties with regards to the dose,safety and the timing of initial and repeat *** side effects of rtPA include retinal pigment epithelial toxicity,increased risk of breakthrough vitreous haemorrhage and systemic *** cases presenting early,pneumatic displacement alone with anti-VEGF may be ***-VEGF monotherapy is a viable treatment option particularly in patients with thinner SMH and those who are unable to posture post pneumatic displacement.

关键词： Submacular hemorrhage(SMH) recombinant tissue plasminogen activator(rtPA) polypoidal choroidal vasculopathy(PCV)

Features of Ciguatera Fish Poisoning Cases in Hong Kong 2004-2007

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Biomedical and Environmental Sciences 2008年第6期21卷 521-527页

作者： CHUN-KWAN wong PATRICIA HUNG KELLIE L. H. LEE TINA MOK THOMAS chung KAI-MAN KAM Biotoxin Laboratory Public Health Laboratory Services Branch Centre for Health Protection Department of Health Shek Kip Mei Kowloon Food and Environmental Hygiene Department Queensway Government Offices

Ohjective To review the clinical features and laboratory investigations of ciguatera patients in Hong Kong between 2004 and 2007 in order to show the timely sampling of implicated fish from ciguatera victims and application of validated mouse bioassay for confirming suspected clinical cases of ciguatera. Methods Diagnosis of the ciguatera victims was based on history of coral fish consumption and clinical presentations stated in official guidelines for clinical diagnosis of ciguatera fish poisoning in Hong Kong. Food remnants of coral fish samples were collected swiftly from ciguatera victims between 2004 and 2007 for ciguatoxins （CTXs） analysis. Results Major clinical symptoms in ciguatera patients included gastrointestinal and neurological effects including limb numbness and diarrhoea, which developed at 0.5 to 15 hours after consumption of fish. In most cases, neurological symptoms were more common than gastrointestinal symptoms. A broad range of attack rate （10%-100%） was observed in each ciguatera outbreak. Validated mouse bioassay on ether extracts of the food remnant samples confirmed that all were CTXs-positive （〈0.5 - 4.3 MU/20 mg ether extract） and directly linked to the corresponding ciguatera cases. Conclusion Consistency between clinical and laboratory analysis for ciguatera poisoning illustrates the application of laboratory mouse bioassay in a timely fashion for confirming ciguatera poisoning cases and implementing effective public health measures. With further improvement in laboratory techniques, features of ciguatera fish poisoning cases can be better defined, Further studies are needed to determine the risk of each class of CTXs （Pacific-, Indian- and Caribbean-CTXs） in Hong Kong.

关键词： Ciguatera poisoning Ciguatoxin Food remnants Hong Kong Mouse bioassay

Measurement of very low-molecular weight metabolites by traveling wave ion mobility and its use in human urine samples

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Journal of Pharmaceutical Analysis 2024年第5期14卷 733-743页

作者： Alongkorn Kurilung Suphitcha Limjiasahapong Khwanta Kaewnarin Pattipong Wisanpitayakorn Narumol Jariyasopit Kwanjeera Wanichthanarak Sitanan Sartyoungkul Stephen Choong chee wong Nuankanya Sathirapongsasuti Chagriya Kitiyakara Yongyut Sirivatanauksorn Sakda Khoomrung Siriraj Center of Research Excellent in Metabolomics and Systems Biology(SiCORE-MSB) Faculty of Medicine Siriraj HospitalMahidol UniversityBangkok10700Thailand Siriraj Metabolomics and Phenomics Center Faculty of Medicine Siriraj HospitalMahidol UniversityBangkok10700Thailand Department of Biomedical Informatics University of Arkansas for Medical SciencesLittle RockAR72205USA SingHealth Duke-NUS Institute of Biodiversity Medicine National Cancer Centre Singapore168583Singapore Waters Pacific Pte. Ltd.Singapore117528Singapore Program in Translational Medicine Faculty of Medicine Ramathibodi HospitalMahidol UniversityBangkok10400Thailand Chakri Naruebodindra Medical Institute Faculty of Medicine Ramathibodi HospitalMahidol UniversitySamut Prakan10540Thailand Department of Medicine Ramathibodi HospitalMahidol UniversityBangkok10400Thailand Department of Biochemistry Faculty of Medicine Siriraj HospitalMahidol UniversityBangkok10700Thailand Center of Excellence for Innovation in Chemistry(PERCH-CIC) Faculty of ScienceMahidol UniversityBangkok10400Thailand

The collision cross-sections(CCS)measurement using ion mobility spectrometry(IMS)in combination with mass spectrometry(MS)offers a great opportunity to increase confidence in metabolite ***,owing to the lack of sensitivity and resolution,IMS has an analytical challenge in studying the CCS values of very low-molecular-weight metabolites(VLMs250 Da).Here,we describe an analytical method using ultrahigh-performance liquid chromatography(UPLC)coupled to a traveling wave ion mobility-quadrupole-time-of-flight mass spectrometer optimized for the measurement of VLMs in human urine *** experimental CCS values,along with mass spectral properties,were reported for the 174 *** experimental data included the mass-to-charge ratio(m/z),retention time(RT),tandem MS(MS/MS)spectra,and CCS *** the studied metabolites,263 traveling wave ion mobility spectrometry(TWIMS)-derived CCS values(TWCCSN2)were reported for the first time,and more than 70%of these were CCS values of *** TWCCSN2 values were highly repeatable,with inter-day variations of<1%relative standard deviation(RSD).The developed method revealed excellent TWCCSN2 accuracy with a CCS difference(DCCS)within±2%of the reported drift tube IMS(DTIMS)and TWIMS CCS *** complexity of the urine matrix did not affect the precision of the method,as evidenced by DCCS within±1.92%.According to the Metabolomics Standards Initiative,55 urinary metabolites were identified with a confidence level of *** these 55 metabolites,53(96%)were *** larger number of confirmed compounds found in this study was a result of the addition of TWCCSN2 values,which clearly increased metabolite identification confidence.

关键词： Very low-molecular-weight metabolites Traveling wave ion mobility Collision cross-section Human urine