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Polycaprolactone/polyvinyl pyrrolidone nanofibers developed by solution blow spinning for encapsulation of chlorogenic acid

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Food Quality and Safety 2022年第2期6卷 265-274页

作者： Yang Cao chaoyi shen Zhichao Yang Zihan Cai Zian Deng Di Wu The Rural Development Academy Zhejiang UniversityHangzhouChina College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhouChina College of Agriculture&Biotechnology Zhejiang UniversityHangzhouChina and Zhejiang University Zhongyuan Institute ZhengzhouChina

Study on the application of nanofibers in food active packaging has been a research hotspot in recent *** this work,solution blow spinning(SBS)was applied to rapidly fabricate the polycaprolactone(PCL),polyvinyl pyrrolidone(PVP),and PCL/PVP nanofibrous films to encapsulate chlorogenic acid(CGA).All films showed uniform and smooth nanofibers,and the Fourier transform infrared spectroscopy and X-ray diffraction proved the success of mixed spinning of PCL and *** the increase of PVP content,the thermal stability of the PCL/PVP nanofibrous films *** PCL/PVP(weight ratio of 4:1)film possessed better mechanical properties than PCL and PVP films be-cause of the stronger fiber-fiber *** addition of PCL endowed the hydrophobic surfaces to the PCL/PVP films,and the PCL/PVP films had better water vapor barrier *** PCL/PVP(4:1)film exhibited the best long-term continuous release of CGA during 72 *** PVP nanofibrous film exhibited no inhibition against Staphylococcus aureus and Escherichia coli due to the low encapsulation efficiency,but the PCL and PCL/PVP films exhibited good antimicrobial *** above results suggested that the nanofibrous fims developed by SBS possess promising prospects in food packaging.

关键词： Solution blow spinning polycaprolactone polvinyl prrolidone chlorogenic acid long-term continuous release

基于双模态图像信息的眼内激光手术联合标定方法

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中国激光 2020年第2期47卷 418-423页

作者：杨帆陈聪沈超逸范立成苏州大学机电工程学院江苏苏州215301

为了获取眼科激光手术中规划手术部位的深度空间信息,搭建了双光纤环形器结构的扫频光相干断层成像(SS-OCT)系统。在样品臂中加入二向色镜,将其与互补金属氧化物半导体(CMOS)相机系统进行整合。对OCT坐标系与相机图像坐标系的几何关系... 详细信息

为了获取眼科激光手术中规划手术部位的深度空间信息,搭建了双光纤环形器结构的扫频光相干断层成像(SS-OCT)系统。在样品臂中加入二向色镜,将其与互补金属氧化物半导体(CMOS)相机系统进行整合。对OCT坐标系与相机图像坐标系的几何关系进行匹配,设计了多角度线性扫描、环形扫描等模式,实现了相机视频图像下任意指定位置的多模式扫描成像。该成像系统的纵向分辨率为13.68μm,横向分辨率为29.8μm,在空气中的成像深度为17.25 mm,测量的最大横向偏差为275μm,最大纵向偏差为70μm,能够满足手术的定位要求。该研究初步实现了眼内指定部位深度信息的快速获取,有望应用于眼科疾病诊断和白内障手术导航。

关键词：医用光学眼科激光手术光相干断层成像系统定位成像深度信息

基于高压静电场处理的樱桃番茄果实贮藏期生理品质及其代谢

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食品科学 2021年第21期42卷 168-176页

作者：杨智超曹阳沈超怡孙崇德吴迪浙江大学农业与生物技术学院浙江杭州310058 浙江大学新农村发展研究院浙江杭州310058

樱桃番茄色泽艳丽,味道酸甜适口,受到消费者欢迎。但樱桃番茄采后容易腐烂变质,且鲜食时其表面致病菌不利于消费者健康。本实验以‘黄妃’樱桃番茄为材料,通过大肠杆菌菌落总数、腐烂率、生理指标测定以及代谢组学分析,研究高压静电场(h... 详细信息

樱桃番茄色泽艳丽,味道酸甜适口,受到消费者欢迎。但樱桃番茄采后容易腐烂变质,且鲜食时其表面致病菌不利于消费者健康。本实验以‘黄妃’樱桃番茄为材料,通过大肠杆菌菌落总数、腐烂率、生理指标测定以及代谢组学分析,研究高压静电场(high voltage electrostatic field,HVEF)处理对樱桃番茄果实贮藏期生理品质及代谢的影响。结果表明,HVEF处理能显著降低果实表面的大肠杆菌菌落总数(P<0.05),并能延缓贮藏期间果实腐烂,且对呼吸速率和质量损失率没有明显影响,果实乙烯释放量在贮藏早期受到抑制。代谢组学分析结果表明,HVEF处理能引起部分代谢物质的含量差异,且存在多种上调和下调模式,其中部分类黄酮和生物碱类物质含量上升。综上,HVEF处理能够降低果实采后腐烂率和表面致病菌数量,并对改善樱桃番茄果实采后品质有一定的作用。

关键词：高压静电场樱桃番茄腐烂率杀菌大肠杆菌生理指标代谢组学分析

负载百里酚的聚己内酯纳米纤维膜制备及保鲜应用研究

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浙江大学学报（农业与生命科学版） 2023年第3期49卷 368-375页

作者：金蓉蔡子涵赵以勒沈超怡杨智超吴迪陈昆松浙江大学农业试验站浙江杭州310058 浙江大学农业与生物技术学院浙江杭州310058 浙江大学生物系统工程与食品科学学院浙江杭州310058 浙江大学中原研究院河南郑州450000

现有的传统活性保鲜包装存在孔隙率低、透气性差等缺点,因此制备纳米级保鲜控释包装材料是果蔬采后贮藏和物流领域中的研究热点。本研究采用溶液吹塑纺丝技术制备负载百里酚(thymol, THY)的聚己内酯(polycaprolactone, PCL)纳米纤维膜,... 详细信息

现有的传统活性保鲜包装存在孔隙率低、透气性差等缺点,因此制备纳米级保鲜控释包装材料是果蔬采后贮藏和物流领域中的研究热点。本研究采用溶液吹塑纺丝技术制备负载百里酚(thymol, THY)的聚己内酯(polycaprolactone, PCL)纳米纤维膜,并通过材料表征和抑菌实验对THY/PCL纳米纤维膜的性能进行评价。结果表明:负载THY后,PCL纳米纤维膜的结晶度降低,热稳定性提高,而水蒸气透过性能、表面疏水性与机械性能未受影响。此外,THY/PCL纳米纤维膜对大肠埃希菌和金黄色葡萄球菌表现出良好的抑菌活性,展现了在果蔬采后保鲜领域良好的应用前景。

关键词：溶液吹塑纺丝纳米纤维百里酚聚己内酯果蔬抑菌

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溶液吹纺技术在生命科学领域中的应用

长沙理工大学学报（自然科学版） 2022年第4期19卷 108-117页

作者：秦自强沈超怡吴迪陈昆松浙江大学农业与生物技术学院浙江杭州310058 浙江大学生物系统工程与食品科学学院浙江杭州310058 浙江大学中原研究院河南郑州450000

溶液吹纺技术是利用高速气流对聚合物溶液拉伸细化使其形成纳米纤维的新技术,具有制备效率高、设备结构简单、制备过程安全性高等特点。本文在介绍溶液吹纺技术的基础上,阐述了溶液吹纺纳米纤维的制备过程并分析了影响吹纺纳米纤维结构... 详细信息

溶液吹纺技术是利用高速气流对聚合物溶液拉伸细化使其形成纳米纤维的新技术,具有制备效率高、设备结构简单、制备过程安全性高等特点。本文在介绍溶液吹纺技术的基础上,阐述了溶液吹纺纳米纤维的制备过程并分析了影响吹纺纳米纤维结构和形态的主要因素,重点介绍了溶液吹纺纳米纤维在组织工程、伤口敷料、药物缓释和果蔬保鲜材料等生命科学领域中的应用,最后展望了溶液吹纺技术的发展趋势。

关键词：溶液吹纺纳米纤维组织工程伤口敷料药物缓释果蔬包装

Planning and Decision-making for Connected Autonomous Vehicles at Road Intersections:A Review

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Chinese Journal of Mechanical Engineering 2021年第5期34卷 26-43页

作者： shen Li Keqi Shu chaoyi Chen Dongpu Cao Department of Civil Engineering Tsinghua UniversityBeijingChina CogDrive Lab Department of Mechanical and Mechatronics EngineeringUniversity of WaterlooWaterlooON N2L 3G1Canada School of Vehicle and Mobility Tsinghua UniversityBeijingChina

Planning and decision-making technology at intersections is a comprehensive research problem in intelligent transportation systems due to the uncertainties caused by a variety of traffic *** wireless communication advances,vehicle infrastructure integrated algorithms designed for intersection planning and decision-making have received increasing *** this paper,the recent studies on the planning and decision-making technologies at intersections are primarily *** general planning and decision-making approaches are presented,which include graph-based approach,prediction base approach,optimization-based approach and machine learning based *** connected autonomous vehicles(CAVs)is the future direction for the automated driving area,we summarized the evolving planning and decision-making methods based on vehicle infrastructure cooperative *** four-way signalized and unsignalized intersection(s)are investigated under purely automated driving traffic and mixed *** study benefit from current strategies,protocols,and simulation tools to help researchers identify the presented approaches’challenges and determine the research gaps,and several remaining possible research problems that need to be solved in the future.

关键词： Planning Decision-making Autonomous intersection management Connected autonomous vehicles

疏水性电荷诱导层析磁性微球分离鸡蛋卵清白蛋白

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湖州师范学院学报 2019年第10期41卷 48-51页

作者：吴立群沈超怡姚晓丹张慧敏顾佳黎湖州师范学院生命科学学院

利用疏水性电荷诱导层析磁性微球从鸡蛋中分离卵清白蛋白,优化卵清白蛋白的分离工艺.辛酸预处理pH为5.8,辛酸加入量为50μL/mL蛋清处理液,磁性微球吸附pH为5.8,洗脱pH为3.8,洗脱时加入NaCl,其浓度为1 mol/L,卵清白蛋白的回收率约为78%,... 详细信息

利用疏水性电荷诱导层析磁性微球从鸡蛋中分离卵清白蛋白,优化卵清白蛋白的分离工艺.辛酸预处理pH为5.8,辛酸加入量为50μL/mL蛋清处理液,磁性微球吸附pH为5.8,洗脱pH为3.8,洗脱时加入NaCl,其浓度为1 mol/L,卵清白蛋白的回收率约为78%,表明疏水性电荷诱导层析磁性微球可以有效分离卵清白蛋白.

关键词：疏水性电荷诱导层析卵清白蛋白鸡蛋

Bushenhuoxue improves cognitive function and activates brain-derived neurotrophic factor-mediated signaling in a rat model of vascular dementia

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Journal of Traditional Chinese Medicine 2020年第1期40卷 49-58页

作者： Wang Wei Zhang Yixin Yu Wentao Gao Weijuan shen Ning Jin Bing Wang Xiangting Fang chaoyi Wang Yanjun General Medical Practice Shuangyushu Community Health Service Center of Haidian DistrictBeijing 100086China Hebei University of Chinese Medicine ShijiazhuangHebei 050200China Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease Shijiazhuang 050091China Acupuncture and Moxibustion Hospital of Chinese Academy of Chinese Medical Sciences Beijing 100700China Department of Geriatrics Xiyuan Hospital of Chinese Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE:To explore the protective mechanisms of the Traditional Chinese Medicine Bushenhuoxue(BSHX)in a rat model of vascular dementia(VD).METHODS:A rat model of VD was developed using bilateral common carotid artery occlusion(BCCAO).Rats were administered BSHX(10.14 or 5.07 g/kg),nimodipine(11.06 mg/kg;positive control),or saline(control)by gavage daily for 30 d *** and memory abilities were assessed using the Morris water *** changes in the hippocampus were observed using light microscopy(hematoxylin and eosin staining)and transmission electron microscopy(TEM).The m RNA and protein expression levels of brain-derived neurotrophic factor(BDNF),tyrosine receptor kinase B(Trk B),phosphatidyl inositol 3-kinase(PI3 K),serine/threonine kinase(AKT),and c AMP response element binding protein(CREB)were measured by real-time polymerase chain reaction(RT-PCR)and Western blot,***:Compared with the sham group,rats with BCCAO exhibited impaired learning and memory abilities(Morris water maze)and showed abnormalities in neuronal morphology(light microscopy)and ultrastructure(TEM)in the *** also had decreased m RNA and protein expressions of BDNF,Trk B,PI3 K,AKT,and CREB in hippocampal tissue(all P<0.05).In rats with BCCAO,administration of BSHX attenuated deficits in learning and memory,improved the morphology and ultrastructure of hippocampal neurons,and enhanced m RNA and protein expression levels of BDNF,Trk B,PI3 K,AKT,and CREB(all P<0.05).CONCLUSION:BSHX may protect hippocampal neurons and improve learning and memory abilities,at least in part via the activation of BDNF/Trk B/PI3 K/AKT/CREB signaling.

关键词： Dementia,vascular Brain-derived neurotrophic factor Phosphatidylinositol 3-kinase Protein serine-threonine kinases Bushenhuoxue prescription

Suppressive effect of Armoracia rusticana and three main glucosinolates on heterocyclic amine generation in roasted fish patties and their potential mechanism analyzed by density functional theory

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Food Science and Human Wellness 2024年

作者： Zhuoming Huang Tiantian Huang Hang Su Zihao Yang Jie Luo Qingwu shen chaoyi Xue Wei Quan College of Food Science and Technology， Hunan Agricultural University State Key Laboratory of Food Science and Resources， Jiangnan University

This study investigated the suppressive effects of Armoracia rusticana (AR) and its three main glucosinolates on both free and bound heterocyclic amines (HAs)， along with their mechanisms of free radical quenching using density functional theory. Fish patties were supplemented with varying concentrations of AR (0.5%～1.5%) and glucosinolates (0.005%～0.015%)， showing a dose-dependent inhibition of HAs and concurrent elimination of free radicals and HAs intermediates. Glucobrassicin demonstrated the highest reactivity， which was verified by frontier orbit analysis and conceptual density functional parameters， consistent with experimental findings. Furthermore， the O-H bond connected to the sulfur atom of glucobrassicin possessed the smallest bond dissociation enthalpy (BDE) value， which indicated that this particular hydrogen atom is most susceptible to react with free radicals. Overall， AR and its glucosinolates， especially glucobrassicin， show promise as natural additives for improving food safety and quality.

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