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Effects of Polysaccharide from Polygala fallax on Apoptosis of B16 Melanoma

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Medicinal Plant 2023年第1期14卷 69-72,75页

作者： chaohao GU Yuanyuan LAN yang MIAO Zhiren YAO Zhiqiang HUANG Kaimei ZHU Shengjiu GU Guilin Medical University Guilin 541101China

[Objectives]The paper was to study the effects of polysaccharide from Polygala fallax on apoptosis of B16 melanoma and its mechanism.[Methods]*** polysaccharide was prepared by water extraction and alcohol precipitation method combined with Sevag *** effects of different concentrations of *** polysaccharide on B16 cell proliferation were detected by MTT *** was detected by Hoechst 33258 staining assay and flow *** expressions of Bcl-2,Bax,Cleaved Caspase-3 mRNA were detected by Real-time PCR *** expressions of Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9,Cleaved Caspase-3,Cleaved Caspase-9,ERK,p-ERK,JNK,p-JNK proteins were detected by Western Blot assay.[Results]*** polysaccharide inhibited the cell proliferation of B16 melanoma in a concentration-dependent manner(24 h IC 50=0.2133 mg/L;48 h IC 50=0.08489μg/mL).*** polysaccharide up-regulated the expressions of Bax,Cleaved Caspase-3 mRNA and protein,down-regulated the expressions of Bcl-2 mRNA and protein,activated Caspase-3,Caspase-8 and Caspase-9,and decreased the levels of p-ERK/ERK and p-JNK/JNK.[Conclusions]*** polysaccharide can inhibit the cell proliferation of B16 melanoma and induce their apoptosis,probably attributed to the regulation of MAPK,Bcl-2 and Caspase family signaling pathways.

关键词： Polygala fallax polysaccharide Melanoma Apoptosis

可弯曲负压鞘联合一次性输尿管软镜与超微经皮肾镜治疗2~3 cm肾结石的疗效比较

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现代泌尿外科杂志 2024年第7期29卷 617-621,626页

作者：林剑峰叶志彬胡力仁梁福律涂建平苗超豪范先明福建中医药大学附属厦门市第三医院泌尿外科福建厦门361100

目的探讨可弯曲负压输尿管软镜鞘(UAS)联合一次性输尿管软镜(FURS)与超微经皮肾镜(SMP)治疗2~3 cm肾结石的治疗效果,为结石患者的治疗选择提供参考。方法回顾性分析2021年9月—2023年9月于福建中医药大学附属厦门市第三医院行手术治疗... 详细信息

目的探讨可弯曲负压输尿管软镜鞘(UAS)联合一次性输尿管软镜(FURS)与超微经皮肾镜(SMP)治疗2~3 cm肾结石的治疗效果,为结石患者的治疗选择提供参考。方法回顾性分析2021年9月—2023年9月于福建中医药大学附属厦门市第三医院行手术治疗的130例2~3 cm肾结石患者的资料,其中采用可弯曲负压UAS联合一次性FURS取石术者68例(UAS联合组),采用SMP者62例(SMP组),比较两组围手术期主要数据指标及术后清石率(SFR)。结果两组手术均顺利完成,UAS联合组与SMP组术后3 d(88.24%vs.90.32%)及1个月(91.18%vs.93.55%)的总SFR、并发症发生率(5.88%vs.9.67%)比较差异无统计学意义(P>0.05)。对于肾盂肾下盏夹角(IPA)<45°的下盏结石患者,UAS联合组SFR明显低于SMP组(57.14%vs.100%,P<0.05)。UAS联合组较SMP组的手术时间更长[(104.94±8.79)min vs.(77.98±6.60)min,P<0.001]、住院费用更高[(23 112.82±1 152.34)元vs.(21 975.84±1 512.24)元,P<0.001],术后血红蛋白下降值更少[(6.71±2.07)g/L vs.(9.81±4.80)g/L,P<0.001]、术后住院时间更短[(3.28±0.51)d vs.(5.58±0.71)d,P<0.001]。UAS联合组术后6、24、48 h视觉模拟评分(VAS)[(6.38±0.69)、(3.62±0.73)、(3.12±0.33)分]均小于SMP组[(7.87±0.88)、(5.81±0.83)、(3.81±0.60)分],差异有统计学意义(P<0.05)。结论对于2~3 cm肾结石两种手术方式均具有较高的SFR,SMP具有手术时间短、住院费用低、下盏结石SFR高等优势,而可弯曲负压UAS联合一次性FURS取石术具有出血少、痛苦小、术后住院时间短等优势。术者可以根据患者病情及其意愿,结合自身经验选择合适的手术方式。

关键词：肾结石可弯曲负压吸引鞘输尿管软镜超微经皮肾镜

Bypassing the limit in volumetric imaging of mesoscale specimens

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Advanced Photonics 2019年第2期1卷 3-4页

作者： chaohao Chen Zhen Liu Dayong Jin University of Technology Sydney Institute for Biomedical Materials and DevicesFaculty of ScienceUltimoNew South WalesAustralia Southern University of Science and Technology Department of Biomedical EngineeringShenzhenChina

The development of light-sheet microscopy(LSM)1,2 has revolutionized high-resolution imaging of biological organisms in three dimensions(3D)with minimal photodamage3 and high temporal resolution,4 therefore allowing long-term observation of biological processes.5,6 The key is to obtain information on subcellular events at both high spatial and temporal resolutions,but the spatial resolution of current LSM methods has been pixel-limited when each frame of the image is collected under a large field of view from an entire large-volume specimen.

关键词： processes. limit dimensions

ON THE CLASSIFICATION OF INITIAL DATAFOR NONLINEAR WAVE EQUATIONS

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Chinese Annals of Mathematics,Series B 2002年第2期23卷 205-208页

作者： GU chaohao(C.H.GU) Institute of Mathematics FudanUniversity China.E-mail:guch

The purpose of the present paper is to call for attention to the following question: Which of the initial data (nonsmall) admit global smooth solutions to the Cauchy problem for nonlinear wave equations. A few cases a... 详细信息

关键词：非线性波动方程原始数据双曲型Liouville方程 Cauchy问题光滑解对偶关系

COMPLETE EXTREMAL SURFACES OF MIXED TYPE IN 3-DIMENSIONAL MINKOWSKI SPACE

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Chinese Annals of Mathematics,Series B 1994年第4期15卷 385-400页

作者： GU chaohao (Institute of Mathematics, Fudan University,Shanghai 200433, China.) FUDAN UNIV INST MATHSHANGHAI 200433PEOPLES R CHINA

ＣＯＭＰＬＥＴＥＥＸＴＲＥＭＡＬＳＵＲＦＡＣＥＳＯＦＭＩＸＥＤＴＹＰＥＩＮ３－ＤＩＭＥＮＳＩＯＮＡＬＭＩＮＫＯＷＳＫＩＳＰＡＣＥ￥ＧＵＣＨＡＯＨＡＯ（ＩｎｓｔｉｔｕｔｅｏｆＭａｔｈｅｍａｔｉｃｓ，ＦｕｄａｎＵｎｉｖｅ... 详细信息

ＣＯＭＰＬＥＴＥＥＸＴＲＥＭＡＬＳＵＲＦＡＣＥＳＯＦＭＩＸＥＤＴＹＰＥＩＮ３－ＤＩＭＥＮＳＩＯＮＡＬＭＩＮＫＯＷＳＫＩＳＰＡＣＥ￥ＧＵＣＨＡＯＨＡＯ（ＩｎｓｔｉｔｕｔｅｏｆＭａｔｈｅｍａｔｉｃｓ，ＦｕｄａｎＵｎｉｖｅｒｓｉｔｙ，Ｓｈａｎｇｈａｉ２００...

关键词：外表面混合型 Minkowski空间平均曲率双曲线偏微分方程

Hysteresis in sap flow and its controlling mechanisms for a deciduous broad-leaved tree species in a humid karst region

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Science China Earth Sciences 2019年第11期62卷 1744-1755页

作者： Rongfei ZHANG Xianli XU Meixian LIU Yaohua ZHANG chaohao XU Ruzhou YI Wei LUO Chris SOULSBY Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Huanjiang Observation and Research Station for Karst Ecosystem Chinese Academy of SciencesHuanjiang 547100China University of Chinese Academy of Sciences Beijing 100049China Northern Rivers Institute School of GeosciencesUniversity of AberdeenAberdeen AB243UFScotlandUK

The critical zone(CZ) represents the intersection of the biosphere with the atmosphere, hydrosphere and *** the hydrological processes and human impact factors on the CZ is fundamental to sustainable water resources management for agroforestry. Transpiration(T) is an important component of terrestrial evapotranspiration(ET), and understanding the time lag(TL) between vegetation transpiration and meteorological factors can improve our knowledge of the mechanisms of vegetation adaptability to a changing environment. However, the controlling factors on the TL remain poorly understood. Therefore, the objective of this study is identifying the temporal dynamics of key controlling factors on the TL, using a typical deciduous broad-leaved tree species(Zenia insigins Chun) of CZ in subtropical humid karst regions. This species is used as an example to explore the characteristics of the TL between SF(sap flow) and hydro-meteorological forcing. Sap flow in these 6 trees was monitored using the thermal dissipation probes(TDP). Results showed that:(1) the peak of diurnal sap flow generally lagged behind PAR but preceded Ta(air temperature), RH(relative humidity) and VPD(vapor pressure deficit), with the mean TL of-67.4 min(PAR), 90.5 min(Ta), 91.6 min(RH) and 92.9 min(VPD), respectively;(2) TL had no significant relationships with the daily mean meteorological factors and soil moisture, but was highly(R^2>0.66) correlated to CRs(changing rates of meteorological factors) in the morning;(3) At seasonal scale, the sap flow rate and TL both were controlled by the seasonality of precipitation and temperature. Overall, the seasonality of the TL was caused by plants' high water loss and strongly active physiological response in hot seasons, leading to close stomata earlier than in cold seasons;(4) The reason why CRs proposed can explain the TL better than mean values of metrological factors is that the CRs considered the distribution and change processes of metro

关键词： Eco-hydrology Soil hydrology Landscape ecology Earth's critical zone

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四川湿地鸟类粪便中的病毒多样性

应用与环境生物学报 2022年第1期28卷 201-207页

作者：文陇英刘方庆杨军戴波杜超豪西南山地濒危鸟类保护四川省高等学校重点实验室乐山师范学院生命科学学院乐山614000 西昌市邛海国家湿地公园保护中心西昌615000

鸟类的病毒主要来自环境,湿地是鸟类重要的栖息地,研究湿地鸟类病毒对于预防传染病传播具有重要意义.对四川省具有代表性的湿地鸟类粪便进行RNA高通量测序分析,共鉴定出275种微生物,其中节杆菌属(Arthrobacter)、微杆菌属(Microbacteri... 详细信息

鸟类的病毒主要来自环境,湿地是鸟类重要的栖息地,研究湿地鸟类病毒对于预防传染病传播具有重要意义.对四川省具有代表性的湿地鸟类粪便进行RNA高通量测序分析,共鉴定出275种微生物,其中节杆菌属(Arthrobacter)、微杆菌属(Microbacterium)、红球菌属(Rhodococcus)、假单胞菌属(Pseudomonas)噬菌体普遍存在,在高原湿地若尔盖和邛海丰度尤其高,另外最高丰度为36的登氏菌属噬菌体(Gordonia phage)也在这两个高原湿地中.病原微生物主要是植物病毒,柏林湖普通鸬鹚(Phalacrocorax carbo)含黄瓜花叶病毒(Cucumber mosaic virus),桫椤湖棕头鸦雀(Paradoxorniswebbianus)和滟澜洲红尾水鸲(Rhyacornisfuliginosus)含青枯菌属病毒(Ralstonia virus),桫椤湖棕头鸦雀还含有危害严重的核盘菌线粒体病毒(Sclerotinia sclerotiorum mitovirus)、稻瘟病菌病毒(Magnaporthe oryzae ourmia-like virus)、甘蓝蚜虫病毒(Brevicoryne brassicae virus)等,尤其还从中发现了与禽流感病毒相似且较高丰度为27的蝙蝠软腐病毒(Bat iflavirus)短序列;南河小白鹭则不含病原微生物.本研究表明桫椤湖棕头鸦雀所含的病原微生物种类最多,提示该湿地公园应为病毒重点检测地点;南河小白鹭及其栖息生境安全;鸟类微生物不仅能反映自身状况,同时与其栖息环境有关.

关键词：湿地鸟类 RNA高通量测序病原微生物

地聚物混凝土-玻璃纤维增强复合材料筋界面黏结行为的梁式试验研究

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工业建筑 2024年第6期54卷 46-53页

作者：毕超豪赖华铭张耿斌颜天佑何瑞肖淑华刘润安卓柯先蔡培德郭永昌广东电网有限责任公司广州供电局广州510000 广东工业大学土木与交通工程学院广州510000

在“双碳”目标下,采用绿色低碳的地聚物混凝土替代高碳排放的硅酸盐水泥混凝土是目前的研究热点之一。以粉煤灰基地聚物混凝土为对象,基于梁式试验开展弯拉作用下地聚物混凝土-玻璃纤维增强复合材料(GFRP)筋界面黏结性能的研究,探明了... 详细信息

在“双碳”目标下,采用绿色低碳的地聚物混凝土替代高碳排放的硅酸盐水泥混凝土是目前的研究热点之一。以粉煤灰基地聚物混凝土为对象,基于梁式试验开展弯拉作用下地聚物混凝土-玻璃纤维增强复合材料(GFRP)筋界面黏结性能的研究,探明了不同混凝土强度、类型对地聚物混凝土-GFRP筋界面黏结性能的影响。结果表明:在与GFRP筋黏结的试验中,由于地聚物混凝土与硅酸盐水泥混凝土存在力学性能的差异,导致两者虽然有相似的破坏模式,但在不同混凝土强度下界面的破坏机制不同,黏结刚度、黏结强度随混凝土变化的规律也不同;对已有的XUE模型和Bimodal模型进行形状系数的修正,通过对比分析,建立适用于地聚物混凝土-GFRP筋的黏结滑移本构模型。

关键词：地聚物混凝土 GFRP筋梁式试验黏结滑移绿色低碳

基于奇/偶互补磁场理论的自起动永磁同步电机两种运行状态研究

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中国电机工程学报 2023年第17期43卷 6862-6873页

作者：阚超豪赵如鹏余银钢合肥工业大学电气与自动化工程学院安徽省合肥市230009 安徽一天电气技术股份有限公司安徽省合肥市230088

基于奇/偶互补磁场理论的新型自起动永磁同步电机(line-start permanent magnet synchronous motor,LSPMSM)根据定子绕组两种联结方式,可分为不同的运行状态。定子仅主绕组接入电网时,电机处于“互补磁场”状态,此时转子同位线圈电阻的... 详细信息

基于奇/偶互补磁场理论的新型自起动永磁同步电机(line-start permanent magnet synchronous motor,LSPMSM)根据定子绕组两种联结方式,可分为不同的运行状态。定子仅主绕组接入电网时,电机处于“互补磁场”状态,此时转子同位线圈电阻的折算值增大,电抗折算值不变,进而在起动初期缩小电机冲击电流的同时,增大起动转矩。定子主绕组与次绕组同时供电时,电机处于常规状态,此时定子绕组电磁性能等效于正规60°相带设计,稳态运行时性能与普通LSPMSM相同。该文分别对新型电机两种运行状态下的工作原理、等效电路和转矩–转速曲线进行分析。建立有限元仿真模型,对样机两种运行状态的堵转性能、起动性能进行研究,并分别研究样机在空载、重载起动条件下的切换过程。样机试验与仿真结果表明,轻载时在牵入同步速度附近切换运行状态,重载时在0.6倍额定转速前切换运行状态,新型电机能够兼顾高起动性能与高稳态性能。

关键词：自起动永磁同步电机奇/偶互补磁场堵转特性切换过程稳态运行特性