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Oxidized LDL upregulated ATP binding cassette transporter-1 in THP-1 macrophages

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Acta Pharmacologica Sinica 2004年第5期 39-44页

作者： chao-ke tang, Guang-hui YI, Jun-hao YANG, Lu-shan LIU,Zuo WANG, Chang-geng RUAN, Yong-zong YANG2Institute of Cardiovascular Disease of Nanhua University, Hengyang 421001, China

AIM: To study the effect of oxidized low density lipoprotein (ox-LDL) on ATP binding cassette transporter A1(ABCA1) in THP-1 macrophages. METHODS: After exposing the cultured THP-1 macrophages to ox-LDL fordifferent periods, cholesterol efflux was determined by FJ-2107P type liquid scintillator. ABCA1 mRNA and proteinlevel were determined by reverse trancriptase-polymerase chain reaction (RT-PCR) and Western blot, *** cholesterol level in THP-1 macrophage foam cells was detected by high performance liquid ***: ox-LDL elevated ABCA1 in both protein and mRNA levels and increased apolipoprotein (apo) A-I-medi-ated cholesterol efflux in a time- and dose-dependent manner. 22(R)-hydroxycholesterol and 9-cis-retinoic acid didsignificantly increase cholesterol efflux in THP-1 macrophage foam cells (Pkedly increased in cholesterol-loadedcells as a result of activation of LXR/RXR. ABCA1 plays an important role in the homeostasis of cholesterol in themacrophages.

关键词： ATP-binding cassette transporters apolipoprotein A-I hydroxycholesterols LDL lipoproteins oxi-dation-reduction macrophages

PAPP-A negatively Regulates ABCA1,ABCG1 and SR-B1 expression by inhibiting LXRa through the IGF-I-mediated signaling pathway

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PAPP-A negatively Regulates ABCA1,ABCG1 and SR-B1 expression...

ABCA1与NPC1在细胞内胆固醇转运中的作用

第11届全国脂质与脂蛋白学术会议

作者： Shi-Lin tang Wu-Jun Chen Kai Yin Guo-Jun Zhao Zhong-Cheng Mo Yun-Cheng Lv Xin-Ping Ouyang chao-ke tang Institute of Cardiovascular Research Key Laboratory for Atherosclerology of Hunan ProvinceLife Science Research CenterUniversity of South China

Pregnancy-associated plasma protein-A(PAPP-A) has been involved in the atherosclerotic process through regulation of local expression of IGF-1 that mediates the activation of the phosphatidylinositol-3(PI3-K ) and Akt kinase(Akt ) signaling cascades which lead to constitutive nitric oxide formation,with its attending vasodilator,antiplatelet and insulin-sensitizing *** addition,IGF-1 may decreased cholesterol efflux through reductions of expression in ABCA1 and SR-B1 by the PI3-K/Akt signaling *** the current study,we examined whether

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生命的化学 2008年第5期28卷 633-636页

作者：李晓旭唐朝克南华大学心血管病研究所动脉硬化学湖南省重点实验室生命科学研究中心衡阳421001

腺苷三磷酸结合盒转运蛋白A1(ATP-binding cassette transporter A1,ABCA1)是血浆高密度脂蛋白(high-density lipoprotein,HDL)颗粒形成之初的限速步骤。ABCA1通过膜泡运输脂质至细胞表面的HDL载脂蛋白的作用机制尚未完全阐明。C型尼曼... 详细信息

腺苷三磷酸结合盒转运蛋白A1(ATP-binding cassette transporter A1,ABCA1)是血浆高密度脂蛋白(high-density lipoprotein,HDL)颗粒形成之初的限速步骤。ABCA1通过膜泡运输脂质至细胞表面的HDL载脂蛋白的作用机制尚未完全阐明。C型尼曼-匹克病(Niemann-Pick disease type C,NPC)主要由NPC1基因突变引起,NPC1蛋白能促进胆固醇和其他脂质从晚期胞内体/溶酶体流入其他细胞结构。ABCA1和NPC1相互作用保持细胞内脂质平衡,与tangier病和N C P病等病理过程密切相关。

关键词：腺苷三磷酸结合盒转运蛋白A1 高密度脂蛋白 NPC1 胆固醇流出

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Effect of Apolipoprotein A-I on ATP Binding Cassette Transporter A1 Degradation and Cholesterol Efflux in THP-1 Macrophage-derived Foam Cells

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 218-226页

作者： chao-ke tang1,2, Guo-Hua tang1, Guang-Hui YI1, Zuo WANG1, Lu-Shan LIU1, Shuang WAN1, Zhong-Hua YUAN1, Xiu-Sheng HE1, Jun-Hao YANG1, Chang-Geng RUAN1, and Yong-Zong YANG1* 1Institute of Cardiovascular Disease of Nanhua University, Hengyang 421001, China 2Xiangya Medical School, Central Southern University, Changsha 410078, China Institute of Cardiovascular Disease of Nanhua University Hengyang 421001 China Xiangya Medical School Central Southern University Changsha 410078 China

Cholesterol-loaded macrophage foam cells are a central componentof atherosclerotic lesions. ATP binding cassette transporter A1 (ABCA1), the defective molecule intangier disease, mediates the efflux of phospholipid and cholesterolfrom cells to apolipoprotein A-I (apoA-I), reversing foam cell formation. This study investigated the effect of apoA-I on ABCA1 degradation and cholesterol efflux in THP-1 macrophage- derived foam cells. After exposure of the cultured THP-1 macrophage-derived foam cells to apoA-I for different time, cholesterol efflux, ABCA1 mRNA and protein levels were determined by FJ-2107P type liquid scintillator, RT-PCR and Western blot, respectively. The mean ABCA1 fluorescence intensity on THP-1 macrophage-derived foam cells was detected by flow cytometry. Results showed that apoA-I markedly increased ABCA1-mediated cholesterol efflux from THP-1 macrophage-derived foam cells. This was accompanied by an increase in the content of ABCA1. ApoA-I did not alter ABCA1 mRNA abundance. Significantly, thiol protease inhibitors increased the level of ABCA1 protein and slowed its decay in THP-1 macrophage-derived foam cells, whereas none of the proteosome-specific inhibitor lactacystin, other protease inhibitors, or the lysosomal inhibitor NH4Cl showed such effects. The apoA-I-mediated cellular cholesterol efflux was enhanced by thiol protease inhibitors. Our results suggested that thiol protease inhibitors mightprovide an alternative way to upregulate ABCA1 protein. Thisstrategy is especially appealing since it may mimic the stabilizingeffect of the natural ligands apoA-I.

关键词： ATP binding cassette transporter A1 apoA-I flow cytometry cholesterol lipoproteins

AOPPs impair Cholesterol Transport and Exacerbate Atherosclerosis by Downregulation of ABCA1 in Apolipoprotein E Knockout Mice

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AOPPs impair Cholesterol Transport and Exacerbate Atheroscle...

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

脂质组学与脂蛋白的新功能——第十二届全国脂质与脂蛋白学术会议

作者：莫中成 Ji Xiao Zhi-feng Long Guang-hui Yi Feng Yao Yu-lin Tan Min Zhang Guo-jun Zhao chao-ke tang 南华大学医学院 Institute of Cardiovascular Research Key Laboratory for Atherosclerology of Hunan ProvinceLife Science Research CenterUniversity of South China Department of Histology and Embryology University of South China

Objects:The aim of this study is to investigate the effects of advanced oxidation protein products(AOPPs)on ATP-binding cassette transporter A1(ABCA1)and ABCG1 expression,atherosclerotic lesion and cholesterol transport in apolipoprotein E knockout(apoE-KO)***:Male 8-week-old apoE-KO mice were fed a high-fat/high-cholesterol(HFHC)*** received

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang Bing Zhang Lanfang Li Xiaomei tang Jinyu Zeng Yige Song chao Xu Kai Zhao Guoqiang Liu Youming Lu Xinyan Li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

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Apolipoprotein A-I Inhibits LPS-Induced As in ApoE-/-Mice through Activation of STAT3 and Upregulation of Tristetraprolin

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Apolipoprotein A-I Inhibits LPS-Induced As in ApoE-/-Mice th...

Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

第11届全国脂质与脂蛋白学术会议

作者： Kai Yin Guang-hui Tu Jing-Feng Li Wu-Jun Chen Guo-Jun Zhao Xin-Ping YangOu Yun-Cheng LV Qian Lu Yuchang Fu Zhi-Sheng Jiang chao-ke tang From Institute of Cardiovascular Research Key Laboratory for Atherosclerology of Hunan ProvinceLife Science Research CenterUniversity of South China Department of Diagnostics The Medical CollegeUniversity of South China Department of pathology Nan Xian Peoples Hospital Department of Nutrition Sciences University of Alabama at BirminghamBirminghamAL 35294-0012USA

Aims:Apolipoprotein A-I(apoA-I),the major component of high-density lipoprotein(HDL),can suppress the lipopolysaccharide(LPS)-induced inflammatory response in *** role of apoA-1 in LPS-induced atherosclerosis in vivo, however,has not been *** the current study,we investigated the effect and mechanism of apoA-I on the development of atherosclerosis in LPS-challenged apoE-/- mice.

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Neural Regeneration Research 2025年第4期20卷 1103-1123页

作者： Xiahong tang Nan Zheng Qingming Lin Yan You Zheng Gong Yangping Zhuang Jiali Wu Yu Wang Hanlin Huang Jun ke Feng Chen Shengli Clinical Medical College of Fujian Medical University Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Emergency Fujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Provincial key Laboratory of Emergency Medicine FuzhouFujian ProvinceChina The Second Department of Intensive Care Unit Fujian Provincial Hospital South BranchFuzhouFujian ProvinceChina

Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological *** preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are *** this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB ***,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by *** results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these *** conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关键词： bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria neuroinflammation oxidative stress pyroptosis reactive oxygen species

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On the robustness of median sampling in noisy evolutionary optimization

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Science China(Information Sciences) 2021年第5期64卷 32-44页

作者： chao BIAN chao QIAN Yang YU ke tang State key Laboratory for Novel Software Technology Nanjing University Shenzhen key Laboratory of Computational Intelligence Department of Computer Science and EngineeringSouthern University of Science and Technology

Evolutionary algorithms(EAs) are a sort of nature-inspired metaheuristics, which have wide applications in various practical optimization problems. In these problems, objective evaluations are usually inaccurate, because noise is almost inevitable in real world, and it is a crucial issue to weaken the negative effect caused by noise. Sampling is a popular strategy, which evaluates the objective a couple of times, and employs the mean of these evaluation results as an estimate of the objective value. In this work, we introduce a novel sampling method, median sampling, into EAs, and illustrate its properties and usefulness theoretically by solving OneMax, the problem of maximizing the number of 1 s in a bit string. Instead of the mean, median sampling employs the median of the evaluation results as an estimate. Through rigorous theoretical analysis on OneMax under the commonly used onebit noise, we show that median sampling reduces the expected runtime exponentially. Next, through two special noise models, we show that when the 2-quantile of the noisy fitness increases with the true fitness, median sampling can be better than mean sampling; otherwise,it may fail and mean sampling can be better. The results may guide us to employ median sampling properly in practical applications.

关键词： evolutionary algorithms noisy optimization median sampling computational complexity runtime analysis