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MaMADS1-MaNAC083 transcriptional regulatory cascade regulates ethylene biosynthesis during banana fruit ripening

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Horticulture Research 2023年第10期10卷 101-111页

作者： Wei Wei Ying-ying Yang chao-jie wu Jian-fei Kuang Jian-ye Chen Wang-jin Lu Wei Shan Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China

The hormone ethylene is crucial in the regulation of ripening in climacteric fruit,such as bananas.The transcriptional regulation of ethylene biosynthesis throughout banana fruit ripening has received much study,but the cascaded transcriptional machinery of upstream transcriptional regulators implicated in the ethylene biosynthesis pathway is still poorly understood.Here we report that ethylene biosynthesis genes,including MaACS1,MaACO1,MaACO4,MaACO5,and MaACO8,were upregulated in ripening bananas.NAC(NAM,ATAF,CUC)transcription factor,MaNAC083,a ripening and ethylene-inhibited gene,was discovered as a potential binding protein to the MaACS1 promoter by yeast one-hybrid screening.Further in vitro and in vivo experiments indicated that MaNAC083 bound directly to promoters of the five ethylene biosynthesis genes,thereby transcriptionally repressing their expression,which was further verified by transient overexpression experiments,where ethylene production was inhibited through MaNAC083-modulated transcriptional repression of ethylene biosynthesis genes in banana fruits.Strikingly,MaMADS1,a ripening-induced MADS(MCM1,AGAMOUS,DEFICIENS,SRF4)transcription factor,was found to directly repress the expression of MaNAC083,inhibiting trans-repression of MaNAC083 to ethylene biosynthesis genes,thereby attenuating MaNAC083-repressed ethylene production in bananas.These findings collectively illustrated the mechanistic basis of a MaMADS1-MaNAC083-MaACS1/MaACOs regulatory cascade controlling ethylene biosynthesis during banana fruit ripening.These findings increase our knowledge of the transcriptional regulatory mechanisms of ethylene biosynthesis at the transcriptional level and are expected to help develop molecular approaches to control ripening and improve fruit storability.

关键词： thereby collective verified

Downregulation of miR-4772-3p promotes enhanced regulatory T cell capacity in malignant pleural effusion by elevating Helios levels

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Chinese Medical Journal 2019年第22期132卷 2705-2715页

作者： Wen-Qing Yu Ning-Fei Ji Cheng-Jing Gu Zhi-Xiao Sun Zheng-Xia Wang Zhong-Qi Chen Yuan Ma Zhen-Zhen wu Yan-Li Wang chao-jie wu Ming-Dong Ding Gui-Hong Dai Juan Yao Rong-Rong Jin Mao Huang Ming-Shun Zhang Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Infectious Diseases Taizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of Pharmacy Taizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of Pathology Taizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of Oncology Taizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of Immunology Nanjing Medical UniversityNanjingJiangsu 210029China

Background:Malignant pleural effusion(MPE)is a complicated condition of patients with advanced tumors.Further dissecting the microenvironment of infiltrated immune cells and malignant cells are warranted to understand the immune-evasion mechanisms of tumor development and progression.Methods:The possible involvement of microRNAs(miRNAs)in malignant pleural fluid was investigated using small RNA sequencing.Regulatory T cell(Treg)markers(CD4,CD25,forkhead box P3),and Helios(also known as IKAROS Family Zinc Finger 2[IKZF2])were detected using flow cytometry.The expression levels of IKZF2 and miR-4772-3p were measured using quantitative real-time reverse transcription polymerase chain reaction.The interaction between miR-4772-3p and Helios was determined using dual-luciferase reporter assays.The effects of miR-4772-3p on Helios expression were evaluated using an in vitro system.Correlation assays between miR-4772-3p and functional molecules of Tregs were performed.Results:Compared with non-malignant controls,patients with non-small cell lung cancer had an increased Tregs frequency with Helios expression in the MPE and peripheral blood mononuclear cells.The verified downregulation of miR-4772-3p was inversely related to the Helios*Tregs frequency and Helios expression in the MPE.Overexpression of miR-4772-3p could inhib让Helios expression in in vitro experiments.However,ectopic expression of Helios in induced Tregs reversed the effects induced by miR-4772-3p overexpression.Additionally,miR-4772-3p could regulate Helios expression by directly targeting IKZF2 mRNA.Conclusion:Downregulation of miR-4772-3p,by targeting Helios,contributes to enhanced Tregs activities in the MPE microenvironment.

关键词： Helios IKAROS family zinc finger 2 Lung cancer Malignant pleural effusion MiR-4772-3p Regulatory T cells

Significant effect of ordered micro-domain on cell boundary phase distribution and demagnetization curve squareness of Sm2Co17-type magnet

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Rare Metals 2024年第4期43卷 1724-1735页

作者： Zhuang Liu Hai-Chen wu chao-Yue Zhang chao-Qun Zhu Guo-Xin Chen Huan-Ming Lu Ren-jie Chen Aru Yan CISRI & NIMTE Joint Innovation Center for Rare Earth Permanent Magnets Ningbo Institute of Materials Technology and EngineeringChinese Academy of Sciences University of Chinese Academy of Sciences Analytical C enter Ningbo Institute of Materials Technology and EngineeringChinese Academy of Sciences

Cu-rich cell boundary phase is difficult to precipitate evenly,resulting in a generally poor demagnetization curve squareness for Fe-rich Sm2Co17-type magnet,which is a key factor limiting the further improvement of magnetic energy product.In this study,we report that nanoscale strip-like ordered micro-domains distributed in1:7H disordered matrix phase of the solid solution precursor is a new factor significantly affecting the precipitation and distribution of the cell boundary phase.Long strip-like and continuous micro-twin structure with twin boundaries neatly perpendicular to the C-axis is observed after sintering treatment.After solution treatment,sequential and long strip-like micro-twins gradually transform into disordered state along the basal plane,forming narrow disordered 1:7H（TbCu7-type structure） phase between the separated strip-like ordered micro-domains.This disordering transformation takes place via broken down of the long strip-like ordered micro-domains,which is accomplished by narrowing along the width direction followed by reduction of the length.Furthermore,a new model revealing the effect of the ordered micro-domains on the formation of the cell boundary phase is proposed.Antiphase boundaries enriched in Cu have already existed in the precursor with long strip-like ordered micro-domains.Therefore,the Cu-rich cell boundary phase acting as strong pinning centers cannot be precipitated homogeneously and distributed continuously after aging,resulting in a poor demagnetization curve squareness of Sm2Co17-type magnet.Our results indicate that significant broken down of the nanoscale ordered micro-domains in solution precursor is the key factor improving the distribution of cell boundary phase in Sm2Co17-type magnets.

关键词： Sm 2 Co 17 magnets Solution precursor Ordered micro-domains Disordering transformation Cellular structure

YKL40 potentially via activating the P38 pathway in decreasing atherosclerotic plaque stability

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中华医学杂志(英文版) 2024年第8期137卷 1003-1005页

作者： Wang chao Liu Yandong Huan Wei wu Jianjin Jin jie Zou Sili Chen Yu Qu Lefeng Department of Vascular and Endovascular Surgery Changzheng Hospital Affiliated to the Naval Medical University Shanghai 200003 China Clinical Research Institute of Integrative Medicine Yueyang Hospital Shanghai University of Traditional Chinese Medicine Shanghai 200437

Atherosclerosis is the main pathological feature of ischemic stroke, which remains a major cause of disability and death worldwide. Vulnerable plaque, was characterized by ulceration, platelet activation, and thrombosis, potentially leading to emboli detachment and stroke. Vascular smooth muscle cells (VSMCs), a major cellular component within lesions, can display pleiotropic phenotypes, including osteogenic or inflammatory phenotypes, which may be harmful in maintaining advanced atherosclerotic plaque stability. The modulation of the function of VSMCs, including proliferation, migration, and synthesizing the extracellular matrix (ECM) and matrix metalloproteinase (MMP), is thought to markedly affect plaque formation and vulnerability. Therefore, it is of considerable significance to drive harmful VSMCs toward VSMCs having beneficial properties to strengthen plaque stability and retard plaque development.

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Active flutter suppression of nonlinear fin-actuator system via inverse system and immersion and invariance method

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Chinese Journal of Aeronautics 2024年第4期37卷 343-362页

作者： Jin LU Zhigang wu chao YANG Chinese Aeronautical Establishment Beijing 100029China School of Aeronautic Science and Engineering Beihang UniversityBeijing 100191China

Structural nonlinearities such as freeplay will affect the stability and even flight safety of the fin-actuator system.There is a lack of a practical method for designing Active Flutter Suppression (AFS) control laws for nonlinear fin-actuator systems.A design method for the AFS controller of the nonlinear all-movable fin-electromechanical actuator system is established by combining the inverse system and the Immersion and Invariance (I&I) theory.First,the composite control law combining the inverse system principle and internal model control is used to offset the nonlinearity and dynamics of the actuator,so that its driving torque can follow the ideal signal.Then,the ideal torque of the actuator is designed employing the I&I theory.The unfavorable oscillation of the fin is suppressed by making the output torque of the actuator track the ideal signal.The simulation results reveal that the proposed AFS method can increase the flutter speed of the nonlinear finactuator system with freeplay,and a set of controller parameters is also applicable for wider freeplay within a certain range.The power required for the actuator does not exceed the power that can be provided by the commonly used aviation actuator.This method can also resist a certain level of noise and external disturbance.

关键词： Aeroelasticity Active flutter suppression Fin-actuator system Freeplay Inverse system Immersion and invariance

Intelligent feedforward gust alleviation based on neural network

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Chinese Journal of Aeronautics 2024年第3期37卷 116-132页

作者： Yitao ZHOU Zhigang wu chao YANG School of Aeronautic Science and Engineering Beihang UniversityBeijing 100191China

This paper proposes a neural network-based intelligent feedforward gust alleviation framework,which includes a neural network identification model and a neural network controller.A neural network training dataset is formed by collecting flight data and the gust data encountered during the aircraft flight.A neural network identification model is first trained to accurately predict the aircraft’s output.Then,based on the output of the identification model and the collected flight data,the parameters of the time-delay neural network controller are obtained through a learning process.The simulation results show that the designed intelligent controller has good gust alleviation effects for both continuous turbulence excitation and discrete gust excitation.For example,when the aircraft is 40000 kg and the flight speed is 0.81Ma,the controller achieves a 67.82%reduction in wingtip acceleration and a 35.90%reduction in center of mass acceleration under continuous turbulence excitation.When considering the measurement uncertainties,such as noise existing in the collected data,the trained controller can still achieve an acceptable gust alleviation effect.Finally,considering a flight in which the aircraft mass is constantly changing,the intelligent controller,which continuously learns from new flight data,maintains a good gust alleviation effect throughout the flight.

关键词： Gust alleviation Intelligent control Feedforward control Neural networks Time-varying aircraft

Iron-Catalyzed Reductive C(aryl)—Si Cross-Coupling of Diaryl Ethers with Chlorosilanes

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Chinese Journal of Chemistry 2024年第6期42卷 578-584页

作者： Pei Liu Baowei wu Xuefeng Cong jie Kong School of Chemistry and Chemical Engineering Northwestern Polytechnical UniversityXi'anShaanxi710072 China Institute of Molecular Plus Department of Chemistry and Haihe Laboratory of Sustainable Chemical TransformationsTianjin University92 Weijin RoadTianjin300072 China

The reductive cross-coupling between C(aryl)—O and Si—Cl bonds is of much importance as a valuable strategy for the construction of C(aryl)—Si bonds but has remained a great challenge.Herein,we report a reductive cross-coupling of diaryl ethers and chlorosilanes via strong electrophilic C(aryl)—O and Si—Cl bonds cleavage by iron catalysis,which constitutes an efficient protocol for the synthesis of a range of functionalized arylsilanes.The combination of low cost FeCl2 as the precatalyst and iPrMgCl as the reductant shows high activity in the successive cleavage of unactivated C(aryl)—O bonds of diaryl ethers and strong electrophilic Si—Cl bonds of chlorosilanes,allowing their cross-coupling in a reductive fashion.The low-valent iron species generated in situ by reduction of FeCl2 with iPrMgCl was proposed,which prefers to initially cleavage the C(aryl)—O bond of diaryl ethers with the chelation help of an o-amide auxiliary.

关键词： Cross-coupling Diaryl ethers Chlorosilanes C—O bond cleavage Iron catalyst 3d transition metals Selectivity Regioselectivity

Resource allocation for D2D-assisted haptic communications

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Digital Communications and Networks 2024年第1期10卷 63-74页

作者： Yan wu chao Yue Yang Yang Liang Ao College of Telecommunication Engineering Army Engineering University of PLANanjing210000China

Haptic communications is recognized as a promising enabler of extensive services by enabling real-time haptic control and feedback in remote environments,e.g.,teleoperation and autonomous driving.Considering the strict transmission requirements on reliability and latency,Device-to-Device(D2D)communications is introduced to assist haptic communications.In particular,the teleoperators with poor channel quality are assisted by auxiliaries,and each auxiliary and its corresponding teleoperator constitute a D2D pair.However,the haptic interaction and the scarcity of radio resources pose severe challenges to the resource allocation,especially facing the sporadic packet arrivals.First,the contentionbased access scheme is applied to achieve low-latency transmission,where the resource scheduling latency is omitted and users can directly access available resources.In this context,we derive the reliability index of D2D pairs under the contention-based access scheme,i.e.,closed-loop packet error probability.Then,the reliability performance is guaranteed by bidirectional power control,which aims to minimize the sum packet error probability of all D2D pairs.Potential game theory is introduced to solve the problem with low complexity.Accordingly,a distributed power control algorithm based on synchronous log-linear learning is proposed to converge to the optimal Nash Equilibrium.Experimental results demonstrate the superiority of the proposed learning algorithm.

关键词： Haptic communications D2D Power control Contention-based access Potential game

Analysis on the causes of massive stranding of Yellow Sea green tide on Lianyungang and Rizhao coasts in 2022

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Journal of Oceanology and Limnology 2024年第3期42卷 816-830页

作者： Lingjuan wu Juan HUANG Yi DING Guiyan LIU Shuai HUANG Song GAO chao YUAN Jiangling XU Ping wu Rui HUANG Ruobing WEN jie MEI North China Sea Marine Forecasting and Hazard Mitigation Center Ministry of Natural ResourcesQingdao 266061China Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation Qingdao 266061China National Engineering Laboratory for Integrated Aero-Space-Ground-Ocean Big Data Application Technology Xi’an 710129China East China Sea Marine Forecasting and Hazard Mitigation Center Ministry of Natural ResourcesShanghai 200136China North China Sea Ecological Center Ministry of Natural ResourcesQingdao 266033China

In 2022,Yellow Sea green tide caused by Ulva prolifera reached a historic minimum and the coastal areas of Shandong Peninsula were less affected.However,the largest amount of seaweed biomass has been washed ashore on Lianyungang and Rizhao coasts since 2015.We studied the development pattern of Yellow Sea green tide in 2022,and analyzed the key environmental factors on the growth and drifting,then discussed the possible reasons that resulted in the massive stranding of green tide biomass in Lianyungang and Rizhao.Results show under the combined influence of the east to southeast winds and currents with shoreward anomalies,green tide drifted to the coastal waters between Shandong and Jiangsu provinces and the distribution areas located westward compared with previous years(2008–2021).Floating U.prolifera rafts from the coastal waters of Binhai and Sheyang drifted continuously into the coastal waters of Lianyungang and Rizhao,providing important supplements for Yellow Sea green tide.Because green tide in 2022 distributed close to the coastal waters,the abundant nutrients might support their continuous high growth rate.In addition,the amount of rainfall around Shandong Peninsula from late June to early July were significantly higher than in previous years,which might promote the development of green tide to some extent.

关键词： Yellow Sea green tide stranding biomass development pattern environmental factor