Downregulation of miR-4772-3p promotes enhanced regulatory T cell capacity in malignant pleural effusion by elevating Helios levels

作     者：Wen-Qing Yu Ning-Fei Ji Cheng-Jing Gu Zhi-Xiao Sun Zheng-Xia Wang Zhong-Qi Chen Yuan Ma Zhen-Zhen Wu Yan-Li Wang Chao-Jie Wu Ming-Dong Ding Gui-Hong Dai Juan Yao Rong-Rong Jin Mao Huang Ming-Shun Zhang Yu Wen-Qing;Ji Ning-Fei;Gu Cheng-Jing;Sun Zhi-Xiao;Wang Zheng-Xia;Chen Zhong-Qi;Ma Yuan;Wu Zhen-Zhen;Wang Yan-Li;Wu Chao-Jie;Ding Ming-Dong;Dai Gui-Hong;Yao Juan;Jin Rong-Rong;Huang Mao;Zhang Ming-Shun

作者机构：Department of Respiratory and Critical Care MedicineThe First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Infectious DiseasesTaizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of PharmacyTaizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of PathologyTaizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of OncologyTaizhou People's Hospital Affiliated to Nantong UniversityTaizhouJiangsu 225300China Department of ImmunologyNanjing Medical UniversityNanjingJiangsu 210029China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2019年第132卷第22期

页      面：2705-2715页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：grants from the Precision Medicine Research of the National Key Research and Development Plan of China(No.2016YFC0905800) National Natural Science Foundation of China(Nos.81970031,81700028) National Science Foundation of Jiangsu Province(Nos.BK20171501,BK20171080,BK20181497) Jiangsu Province's Young Medical Talent Program,China(No.QNRC2016600) Jiangsu Provincial Health and Family Planning Commission Foundation(No.Q2017001) Taizhou Municipal Science and Technology Bureau(No.TS201726) Jiangsu Provincial Medical Youth Talent(No.QNRC2016508) 

主　　题：Helios IKAROS family zinc finger 2 Lung cancer Malignant pleural effusion MiR-4772-3p Regulatory T cells 

摘      要：Background:Malignant pleural effusion(MPE)is a complicated condition of patients with advanced *** dissecting the microenvironment of infiltrated immune cells and malignant cells are warranted to understand the immune-evasion mechanisms of tumor development and ***:The possible involvement of microRNAs(miRNAs)in malignant pleural fluid was investigated using small RNA *** T cell(Treg)markers(CD4,CD25,forkhead box P3),and Helios(also known as IKAROS Family Zinc Finger 2[IKZF2])were detected using flow *** expression levels of IKZF2 and miR-4772-3p were measured using quantitative real-time reverse transcription polymerase chain *** interaction between miR-4772-3p and Helios was determined using dual-luciferase reporter *** effects of miR-4772-3p on Helios expression were evaluated using an in vitro *** assays between miR-4772-3p and functional molecules of Tregs were ***:Compared with non-malignant controls,patients with non-small cell lung cancer had an increased Tregs frequency with Helios expression in the MPE and peripheral blood mononuclear *** verified downregulation of miR-4772-3p was inversely related to the Helios*Tregs frequency and Helios expression in the *** of miR-4772-3p could inhib让Helios expression in in vitro ***,ectopic expression of Helios in induced Tregs reversed the effects induced by miR-4772-3p ***,miR-4772-3p could regulate Helios expression by directly targeting IKZF2 ***:Downregulation of miR-4772-3p,by targeting Helios,contributes to enhanced Tregs activities in the MPE microenvironment.