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Genome-Wide Analysis of DNA Methylation in Soybean

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Molecular Plant 2013年第6期6卷 1961-1974页

作者： Qing-Xin song xiang lu Qing-Tian Li Hui chen Xing-Yu Hu biao ma Wan-Ke zhang shou-yi chen jin-song zhang State Key Lab of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Cytosine methylation is an important mechanism for dynamical regulation of gene expression and trans- posable element （TE） mobility during plant developmental processes. Here, we identified the transcription start sites of genes using high-throughput sequencing and then analyzed the DNA methylation status in soybean roots, stems, leaves, and cotyledons of developing seeds at single-base resolution. Profiling of DNA methylation in different organs revealed 2162 differentially methylated regions among organs, and a portion of hypomethylated regions were correlated with high expression of neighboring genes. Because of the different distribution of class I TEs （retrotransposons） and class II TEs （DNA transposons）, the promoters of the lowest-expressed genes showed higher levels of CG and CHG methyla- tion but a lower level of CHH methylation. We further found that the CHH methylation level of class II TEs was higher than class I TEs, possibly due to the presence of more smRNAs in class II TEs. In cotyledons of developing seeds, smRNA abundance was roughly positively correlated with hypermethylated regions but negatively related to hypomethylated regions. These studies provide significant insights into the complicated interplays among DNA methylation, smRNA abundance, TE distribution, and gene expression in soybean.

关键词： soybean DNA methylation, differentially methylated regions, organs.

Identification of Rice Ethylene-Response Mutants and Characterization of MHZ71OsEIN2 in Distinct Ethylene Response and yield Trait Regulation

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Molecular Plant 2013年第6期6卷 1830-1848页

作者： biao ma Si-Jie He Kai-Xuan Duan Cui-Cui yin Hui chen chao yang Qing Xiong Qing-Xin song xiang lu Hao-Wei chen dVan-Ke zhang Tie-Gang lu shou-yi chen jin-song zhang State Key Lab of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement Chinese Academy of Agricultural 5ciences Beijing100081 China

Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, includ- ing ethylene-inhibited root growth and ethylene-promoted coleoptile elongation, which is different from the ethylene triple-response phenotype in Arabidopsis. We establish an efficient system for screening and a set of rice mutants have been identified. Genetic analysis reveals that these mutants form eight complementation groups. All the mutants show insensitivity or reduced sensitivity to ethylene in root growth but exhibit differential responses in cole0ptile growth. One mutant group mhz7 has insensitivity to ethylene in both root and coleoptile growth. We identified the corresponding gene by a map-based cloning method. MHZ7 encodes a membrane protein homologous to EIN2, a central component of ethylene signaling in Arabidopsis. Upon ethylene treatment, etiolated MHZ7-overexpressing seedlings exhibit enhanced coleoptiie elongation, increased mesocotyl growth and extremely twisted short roots, featuring enhanced ethylene- response phenotypes in rice. Grain length was promoted in MHZ7-transgenic plants and 1000-grain weight was reduced in mhz7 mutants. Leaf senescent process was also affected by MHZ7 expression. manipulation of ethylene signaling may improve adaptive growth and yield-related traits in rice.

关键词： rice ethylene-response mutant MHZ7 yield traits senescence.

CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert maarten Boonekamp marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai yijian Cai yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei chao Auttakit Chatrabhuti yimin Che Yuzhi Che Bin chen Danping chen Fuqing chen Fusan chen Gang chen Guoming chen Hua-Xing chen Huirun chen jinhui chen Ji-Yuan chen Kai chen mali chen Mingjun chen Mingshui chen Ning chen Shanhong chen Shanzhen chen Shao-Long chen Shaomin chen Shiqiang chen Tianlu chen Wei chen xiang chen Xiaoyu chen Xin chen Xun chen Xurong chen Ye chen ying chen Yukai chen Zelin chen Zilin chen Gang chen Boping chen Chunhui chen Hok Chuen cheng Huajie cheng Shan cheng Tongguang cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP jinR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

A PP2C-1 Allele Underlying a Quantitative Trait Locus Enhances Soybean l O0-Seed Weight

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Molecular Plant 2017年第5期10卷 670-684页

作者： xiang lu Qing Xiong Tong cheng Qing-Tian Li Xin-Lei Liu ying-Dong Bi Wei Li Wan-Ke zhang biao ma Yong-Cai Lai Wei-Guang Du Wei-Qun man shou-yi chen jin-song zhang State Key Lab of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Institute of Soybean Research Heilongjiang Provincial Academy of Agricultural Sciences Harbin 150086 China Institute of Farming and Cultivation Heilongjiang Provincial Academy of Agricultural Sciences Harbin 150086 China These authors contributed equally to this article

Cultivated soybeans may lose some useful genetic loci during domestication. Introgression of genes from wild soybeans could broaden the genetic background and improve soybean agronomic traits. In this study, through whole-genome sequencing of a recombinant inbred line population derived from a cross between a wild soybean ZYD7 and a cultivated soybean HN44, and mapping of quantitative trait loci for seed weight, we discovered that a phosphatase 2C-1 （PP2C-1） allele from wild soybean ZYD7 contributes to the increase in seed weight/size. PP2C-1 may achieve this function by enhancing cell size of integument and activating a subset of seed trait-related genes. We found that PP2C-1 is associ- ated with GmBZR1, a soybean ortholog of Arabidopsis BZR1, one of key transcription factors in brassi- nosteroid （BR） signaling, and facilitate accumulation of dephosphorylated GmBZR1. In contrast, the PP2C-2 allele with variations of a few amino acids at the N-terminus did not exhibit this function. More- over, we showed that GmBZR1 could promote seed weight/size in transgenic plants. Through analysis of cultivated soybean accessions, we found that 40% of the examined accessions do not have the PP2C-1 allele, suggesting that these accessions can be improved by introduction of this allele. Taken together, our study identifies an elite allele PP2C-1, which can enhance seed weight and/or size in soybean, and pinpoints that manipulation of this allele by molecular-assisted breeding may increase production in soybean and other legumes/crops.

关键词： soybean lO0-seed weight PP2C-1 BZR1

Azvudine is a thymus-homing anti-SARS-CoV-2 drug effective in treating COVID-19 patients

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Signal Transduction and Targeted Therapy 2022年第1期7卷 228-242页

作者： jin-Lan zhang Yu-Huan Li lu-lu Wang Hong-Qi Liu Shuai-Yao lu Yong Liu Ke Li Bin Liu Su-Yun Li Feng-Min Shao Kun Wang Ning Sheng Rui Li jin-jin Cui Pei-Chun Sun Chun-Xia ma Bo Zhu Zhe Wang Yuan-Hao Wan Shi-Shan Yu Yongsheng Che chao-yang Wang chen Wang Qiangqian zhang Li-Min Zhao Xiao-Zhong Peng Zhenshun cheng Jun-biao Chang Jian-Dong Jiang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Institute of Medicinal Biotechnology Chinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Institute of Medical Biology Chinese Academy of Medical Science and Peking Union Medical CollegeKunming 650031 YunnanChina Genuine Biotech.Inc. Pingdingshan 467000 HenanChina Department of Respiratory Medicine Zhongnan Hospital of Wuhan UniversityWuhan Research Center for Infectious Diseases and CancerWuhan 430071 HubeiChina The First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000 HenanChina Department of Internal Medicine Henan Provincial Peoples HospitalZhengzhou 450003 HenanChina Henan Key Laboratory of Organic Functional Molecule and Drug Innovation School of Chemistry and Chemical EngineeringHenan Normal UniversityXinxiang 453007 HenanChina National Clinical Research Center for Respiratory Diseases Chinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100005China School of Chemistry Zhengzhou UniversityZhengzhou 450001 HenanChina

Azvudine(FNC)is a nucleoside analog that inhibits HIV-1 RNA-dependent RNA polymerase(RdRp).Recently,we discovered FNC an agent against SARS-CoV-2,and have taken it into Phase III trial for COVID-19 *** monophosphate analog inhibited SARS-CoV-2 and HCoV-OC43 coronavirus with an EC_(50) between 1.2 and 4.3 μM,depending on viruses or cells,and selective index(SI)in 15-83 *** administration of FNC in rats revealed a substantial thymus-homing feature,with FNC triphosphate(the active form)concentrated in the thymus and peripheral blood mononuclear cells(PBMC).Treating SARS-CoV-2 infected rhesus macaques with FNC(0.07 mg/kg, qd,orally)reduced viral load,recuperated the thymus,improved lymphocyte profiles,alleviated in flammation and orga n damage,and lessened grou nd・glass opacities in chest *** gle-cell seque ncing suggested the promotion of thymus function by FNC.A randomized,single-arm clinical trial of FNC on compassionate use(n=31)showed that oral FNC(5 mg,qd)cured all COVID-19 patients,with 100%viral ribonucleic acid negative conversion in 3.29±2.22 days(range:1-9 days)and 100%hospital discharge rate in 9.00±4.93 days(range:2-25 days).The side-effect of FNC is minor and transient dizziness and nausea in 16.12%(5/31)***,FNC might cure COVID-19 through its anti-SARS-CoV-2 activity concentrated in the thymus,followed by promoted immunity.

关键词： patients inhibited concentrated

Association of genotypes of rs671 within ALDH2 with risk for gastric cardia adenocarcinoma in the Chinese Han population in high-and low-incidence areas

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Cancer Biology & Medicine 2017年第1期14卷 60-65页

作者： Lian-Qun zhang Xin song Xue-Ke Zhao Jia Huang Peng zhang lu-Wen Wang Hui Meng Jian-Wei Ku Guo-Qiang Kong Tao Jiang Xin-Min Li Xiao-Long Lv Teng ma Guo Yuan Min-Jie Wu shou-Jia Hu Shuang Lv Tang-Juan zhang Ling-Fen Ji Zong-Min Fan Neng-chao Wang Yao-Wen zhang Fu-You Zhou Li-Dong Wang Henan Key Laboratory for Esophageal Cancer Research of the First Affiliated Hospital Zhengzhou UniversityZhengzhou 450052China Anyang Tumor Hospital Anyang 455000China Cancer Research Center Xinxiang Medical UniversityXinxiang 453003China

Objective: This study aimed to determine if gastric cardia adenocarcinoma(GCA) risk was associated with the lys(A or *2) allele at the rs671(glu504lys) polymorphism within the aldehyde dehydrogenase 2(ALDH2) gene in a Chinese Han population. We also aimed to investigate ALDH2 genotypic distributions between subjects from high- and low-incidence areas for both GCA and esophageal squamous cell carcinoma(ESCC).Methods: We designed a case-control study including 2,686 patients with GCA and 3,675 control subjects from high- and lowincidence areas for both GCA and ESCC in China. Taq man allele discrimination assay was used to genotype the rs671 polymorphism. χ~2 test and binary logistic regression analysis were used to estimate the odds ratios for the development of GCA,and multivariate ordinal logistic regression was used to analyze ALDH2 genotypic distributions among different ***: Compared with ALDH2*1/*1 homozygotes, ALDH2*1/*2 and ALDH2*2/*2 carriers did not increase the risk for GCA in the Chinese Han population(P>0.05). Interestingly, the ratio of homozygous or heterozygous ALDH2 *2 carriers in highincidence areas for both GCA and ESCC was lower than that in low-incidence areas(Plusions: Genotypes of rs671 at ALDH2 may not increase GCA susceptibility in Chinese Han populations. In addition, the ALDH2 genotypic distribution differs between Chinese Han populations from high- and low-incidence areas for both GCA and ESCC. Our findings may shed light on the possible genetic mechanism for the dramatic geographic differences of GCA occurrence in China.

关键词： Gastric cardia adenocarcinoma rs671 ALDH2

Consensus statement on layout and delivery of automatic external defibrillator in China

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Journal of Hainan Medical University 2020年第15期26卷 18-23页

作者： Yu Cao song chen Yan-Fen Chai Xiao-Hui chen Yu-Guo chen Shao-Wen cheng Wen-Wei cheng Bang-Han Ding yang Deng Jie Fu Bang-Jiang Fang mai-ying Fan Li Gui Ping Gong Wei Guo Shu-Bin Guo Liang Huang Ji-Yuan Han Zhong Ji Xian-Fei Ji Xin-Cai Ji Hong-Xu jin Xin-Jun Jiang Wang-xiang Jiang Jia-Liang Liu Xiao-Ran Liu Zhi Liu Chuan-Zhu Lv Feng lu Tan-Shi Li Xin Li Yong Li Shang-lun Li Shuang-Ming Li Xiao-Gang Li Bao-Jun Li Pei-Wu Li Chun-Sheng Li Zhi-Jun Li Chang-luo Li xiang-Min Li yin-Ping Li Zong-Jun lu Zu-Xun lu Zhong-Qiu lu Zhi-Qian luo Xiao-Xing Liao Jian-Bo Lei Tao ma Yu ma Yue-Feng ma Qing-Bian ma Lin-Hao ma Jun-Qi ma Shi-Nan Nie Jie-Miao Ou-yang Peng Peng Qiao Pei Shu-Ming Pan Guo-Zhong Qing Li-Jie Qin Wu-ying Qiao Fei Shao Wei song Xing-Yue song Zhen-Ju song Rong-Ju Sun De-Ya Shang Du-Juan Sha Gui-Lan Tao Guo-Gang Tian ying-Ping Tian Jian-Guang Tian chao-yang Tong Zi-Ren Tang Meng-Xiong Tang Guo-Ping Wu Zhi Wan Tao Wang Xin Wang Peng Wang Zhong Wang Tong Wang Ri-Xing Wang yi-Xin Wang Xiao-Dan Wang Ling Wu Wei Wei Hua xiang Feng Xu Jun Xu Jie Xu Miao-Rong Xie Shuo-Gui Xu Tie Xu Feng Yu Wen yi Li-Shan yang Shi-Jiao Yan jin-Jian Yao shou-Chun Yan Xue-Zhong Yu Xian-Zhi Ye Xian-Liang Yan Fu-Bing Zhou Rong-Bin Zhou Ming Zhou Qiang Zhou jin Zhou Ping Zhou Chang-Ju Zhu Hua-Dong Zhu Ji-Hong Zhu Hong zhang mao zhang Hua zhang Na zhang Guo-Qiang zhang Guo-Xiu zhang jin-song zhang Wen-Wu zhang Wen-Zhong zhang Jian-Feng zhang yi-Min Zhu Jun Zeng Yuan Zheng Zhi-Jie Zheng Shen Zhao Yan Zhao Min Zhao Bin Zhao Xiao-Dong Zhao Xiao-jing Zhao Hong-Ke Zeng Sheng-Qiang Zou The Chinese Society of Emergency Medicine Research Unit of Island Emergency Medicine Chinese Academy of Medical SciencesHainan Medical University Key Laboratory of Emergency and Trauma of Ministry of Education Hainan Medical UniversityHaikouHainanChina Emergency and Trauma College Hainan Medical UniversityHaikouHainanChina Department of Emergency Hainan Clinical Research Center for Acute and Critical DiseasesThe Second Affiliated Hospital of Hainan Medical UniversityHaikouHainanChina Key Laboratory of Hainan Trauma and Disaster Rescue Key Laboratory of Trauma of Hainan Medical University

*** Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac *** is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symptoms that are atypical and not easily detected[1].According to a recent study,nearly 544000 patients suffer from SCD every year in China[2].

关键词： cardiac death respiratory

双支撑颧骨截骨降低术在面部轮廓整形中的应用

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中华整形外科杂志 2018年第1期34卷 20-23页

作者：卢建建徐家杰张超解芳杨莉亚吴欢欢孙恒马刘红杨彪杨璐滕利中国医学科学院北京协和医学院整形外科医院颅颌面二中心北京100144 广东省人民医院整形外科

目的探讨一种提供稳定支撑的口内入路颧骨截骨降低术的新方法。方法根据颧骨颧弓复合体及咀嚼肌的解剖学特点，设计前后支撑的颧骨颧弓截骨术，包括颧骨体部的L形截骨术和颧弓根部的斜形截骨术，参考颧骨颧弓突出程度，整体适度向内、... 详细信息

目的探讨一种提供稳定支撑的口内入路颧骨截骨降低术的新方法。方法根据颧骨颧弓复合体及咀嚼肌的解剖学特点，设计前后支撑的颧骨颧弓截骨术，包括颧骨体部的L形截骨术和颧弓根部的斜形截骨术，参考颧骨颧弓突出程度，整体适度向内、向上推移，于颧骨体部形成第1支撑、颧弓根部形成第2支撑的前后双支撑结构，达到颧骨颧弓降低的效果。结果2015年1月至2017年1月，应用双支撑颧骨截骨降低手术方法治疗颧骨高突患者76例，随访10～12个月，均获得满意疗效，未发生骨不连等并发症。结论双支撑颧骨截骨降低术矫正颧骨高突畸形，可以有效地降低颧骨颧弓高度，预防骨不连、面部软组织松垂等并发症，是一种比较理想的手术方法。

关键词：颧骨骨不连面中部松垂

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滇西北中甸普朗斑岩铜矿流体包裹体初步研究

地球化学 2007年第5期36卷 467-478页

作者：王守旭张兴春秦朝建石少华冷成彪陈衍景中国科学院地球化学研究所矿床地球化学国家重点实验室

普朗斑岩铜矿位于三江地区义敦岛弧南端的中甸弧,是在晚三叠世甘孜-理塘洋盆向西俯冲过程中形成的一个大型-超大型矿床。通过显微测温学和激光拉曼谱学研究,发现普朗斑岩铜矿含矿石英脉中广泛发育富液相包裹体、含CO2三相/两相包裹体、... 详细信息

普朗斑岩铜矿位于三江地区义敦岛弧南端的中甸弧,是在晚三叠世甘孜-理塘洋盆向西俯冲过程中形成的一个大型-超大型矿床。通过显微测温学和激光拉曼谱学研究,发现普朗斑岩铜矿含矿石英脉中广泛发育富液相包裹体、含CO2三相/两相包裹体、含子晶多相包裹体。子晶种类复杂,盐类矿物主要有石盐、钾盐、方解石、石膏,金属矿物主要为黄铜矿和赤铁矿。流体包裹体中发现有4种流体,即高盐度岩浆流体、含CO2低盐度流体、中等盐度流体以及少量低盐度水溶液。高盐度岩浆流体的均一温度范围457～245℃,均一压力范围(259.33～25.43)×105Pa,盐度为34%～54%NaCl,密度为1.12～1.07g/cm3,富含Na、K、Ca、Cu、Fe、CO2、Cl、S等成分。原始岩浆流体可能是由中酸性岩浆上升至中间岩浆房后直接出溶而成,并汇聚在岩浆房的顶部。在甘孜-理塘洋盆的俯冲过程中,岩浆流体在流体内压和洋盆俯冲所产生的主压应力场的共同作用下,可以间歇性地从岩浆房上涌,为早期岩浆活动(226±3)～(228±3)ma所形成的岩浆-热液系统提供了持续的矿质和热源。含CO2低盐度流体与高盐度岩浆流体的相分离作用是导致普朗斑岩铜矿主成矿期网脉状-浸染状矿化的主要原因。

关键词：流体包裹体普朗斑岩铜矿中甸滇西北