Identification of Rice Ethylene-Response Mutants and Characterization of MHZ71OsEIN2 in Distinct Ethylene Response and Yield Trait Regulation

作     者：Biao Ma Si-Jie He Kai-Xuan Duan Cui-Cui Yin Hui Chen Chao Yang Qing Xiong Qing-Xin Song Xiang LU Hao-Wei Chen dVan-Ke Zhang Tie-Gang Lu Shou-Yi Chen Jin-Song Zhang 

作者机构：State Key Lab of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement Chinese Academy of Agricultural 5ciences Beijing100081 China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第6期

页      面：1830-1848页

核心收录：

学科分类：0710[理学-生物学] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：the National Natural Science Foundation of China the National Key Basic Research Project the National Transgenic Research Project 

主　　题：rice ethylene-response mutant MHZ7 yield traits senescence. 

摘      要：Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, includ- ing ethylene-inhibited root growth and ethylene-promoted coleoptile elongation, which is different from the ethylene triple-response phenotype in Arabidopsis. We establish an efficient system for screening and a set of rice mutants have been identified. Genetic analysis reveals that these mutants form eight complementation groups. All the mutants show insensitivity or reduced sensitivity to ethylene in root growth but exhibit differential responses in cole0ptile growth. One mutant group mhz7 has insensitivity to ethylene in both root and coleoptile growth. We identified the corresponding gene by a map-based cloning method. MHZ7 encodes a membrane protein homologous to EIN2, a central component of ethylene signaling in Arabidopsis. Upon ethylene treatment, etiolated MHZ7-overexpressing seedlings exhibit enhanced coleoptiie elongation, increased mesocotyl growth and extremely twisted short roots, featuring enhanced ethylene- response phenotypes in rice. Grain length was promoted in MHZ7-transgenic plants and 1000-grain weight was reduced in mhz7 mutants. Leaf senescent process was also affected by MHZ7 expression. Manipulation of ethylene signaling may improve adaptive growth and yield-related traits in rice.