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富氢液对大鼠全脑缺血再灌注时外周血调节性T淋巴细胞的影响

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中华麻醉学杂志 2014年第3期34卷 370-372页

作者：李茜曾秋婷于攀朱长松刘韬陈兴东段满林徐建国南京大学医学院南京军区南京总医院汤山分院麻醉科 211131 南京大学医学院南京军区南京总医院麻醉科

目的：评价富氢液对大鼠全脑缺血再灌注时外周血调节性T淋巴细胞（Treg细胞）的影响。方法雄性SD大鼠77只，2～3月龄，体重260～300 g ，采用随机数字表法，将其分为3组：假手术组（S组，n＝11）、缺血再灌注组（I/R组，n＝33）和富氢... 详细信息

目的：评价富氢液对大鼠全脑缺血再灌注时外周血调节性T淋巴细胞（Treg细胞）的影响。方法雄性SD大鼠77只，2～3月龄，体重260～300 g ，采用随机数字表法，将其分为3组：假手术组（S组，n＝11）、缺血再灌注组（I/R组，n＝33）和富氢液组（H组，n＝33）。I/R组和H组采用四血管阻断法制备大鼠全脑缺血再灌注损伤模型，阻断双侧颈总动脉15 min后恢复血流灌注。H组于再灌注即刻和再灌注6 h时腹腔注射0.6 mmol/L富氢液5 ml/kg ，S组和I/R组腹腔注射等容量生理盐水。S组于缺血前（T0）、I/R组和H2组分别于再灌注6 h （T1）、24 h （T2）和72 h （T3）时，取7只大鼠，取外周静脉血样，测定Treg细胞数量，然后处死取脾组织，测定转化生长因子β1（TGF-β1）含量。分别于上述时点处死4只大鼠，取全脑组织，观察海马CA1区锥体细胞形态，并计数锥体细胞。结果与S组比较，I/R组T1-3时海马CA1区锥体细胞计数和外周血Treg细胞数量均减少，脾组织TGF-β1含量降低（ P＜0.05）；与I/R组比较，H组T2-3时海马CA1区锥体细胞计数和外周血Treg细胞数量均增多，T1-3时脾组织TGF-β1含量升高（ P＜0.05），锥体细胞病理学损伤减轻。结论富氢液减轻大鼠全脑缺血再灌注损伤的机制可能与增加外周血Treg细胞数量，促进TGF-β1的分泌有关。

关键词： T淋巴细胞,调节性氢再灌注损伤脑

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采后常温贮藏下菠萝罹病果实转录组测序分析

热带作物学报 2024年第11期45卷 2287-2297页

作者：张梦卓纳琦婷朱长松曹慧刘诗颖史学群孟兰环海南大学食品科学与工程学院海南海口570228

菠萝在常温贮藏过程中易发生黑心病,为了探索黑心病的发生机制,选取菠萝黑心病初期果实的发病部位和健康部位,每个部位选取3个样本分别进行测转录组RNA-Seq分析,对差异基因进行GO、KEGG、各代谢通路基因表达聚类分析和RT-qPCR验证,对比... 详细信息

菠萝在常温贮藏过程中易发生黑心病,为了探索黑心病的发生机制,选取菠萝黑心病初期果实的发病部位和健康部位,每个部位选取3个样本分别进行测转录组RNA-Seq分析,对差异基因进行GO、KEGG、各代谢通路基因表达聚类分析和RT-qPCR验证,对比病部(IB)和健部(WT)的差异基因表达量。通过分析发现,IB vs WT共有1037个差异基因,其中上调886个,下调130个。GO和KEGG分析发现氨基酸生物合成差异基因最显著,而且差异基因数量占比大;通过聚类分析和RT-qPCR验证,糖代谢路径发现果实发病后糖酵解、三羧酸循环(TCA)和氧化磷酸化过程中相关酶基因表达量显著上调;植物应对外界环境信号通路发现抗性酶基因表达量显著下调,致病蛋白基因表达量显著上调,抗病蛋白基因表达量显著下调;抗氧化物质通路发现谷胱甘肽合成基因表达量显著下调,类黄酮生物合成相关基因表达量显著上调,L-抗坏血酸过氧化物酶(APX)基因表达量显著下调,而L-抗坏血酸氧化酶(AOX)同系物基因表达量显著上调。RNA-Seq和RT-qPCR分析结果表明,菠萝组织通过显著上调糖代谢关键基因加速果实病部糖代谢、显著上调表达信号传导关键通路MAPK途径中的关键基因以及显著下调抗氧化物质等代谢通路变化而导致黑心病的发生。通过这些结果可以丰富菠萝黑心病发生的基因网络,为后续菠萝黑心病相关基因筛选提供信息参考。

关键词：常温贮藏菠萝黑心病 RNA-Seq分析

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A Fast Tongue Detection and Location Algorithm in Natural Environment

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Computers, Materials & Continua 2022年第12期73卷 4727-4742页

作者： Lei zhu Guojiang Xin Xin Wang changsong Ding Hao Liang Qilei Chen School of Informatics Hunan University of Chinese MedicineChangsha410208China TCM Big Data Analysis Laboratory of Hunan Changsha410208China Department of Computer Science University of Massachusetts LowellLowell01854USA

The collection and extraction of tongue images has always been an important part of intelligent tongue *** present,the collection of tongue images generally needs to be completed in a sealed,stable light environment,which is not conducive to the promotion of extensive tongue image and intelligent tongue *** response to the problem,a newalgorithm named GCYTD(GELU-CA-YOLO Tongue Detection)is proposed to quickly detect and locate the tongue in a natural environment,which can greatly reduce the restriction of the tongue image collection *** algorithm is based on the YOLO(You Only Look Once)V4-tiny network model to detect the ***,the GELU(Gaussian Error Liner Units)activation function is integrated into the model to improve the training speed and reduce the number of model parameters;then,the CA(Coordinate Attention)mechanism is integrated into the model to enhance the detection precision and improve the failure tolerance of the *** with the other classical algorithms,Experimental results show thatGCYTD algorithm has a better performance on the tongue images of all types in terms of training speed,tongue detection speed and detection precision,*** lighter model can contribute on deploying the tongue detection model on small mobile terminals.

关键词： Tongue detection YOLO V4-tiny CA mechanism GELU

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右美托咪定治疗腹部手术患者苏醒期躁动

中国基层医药 2015年第24期22卷 3698-3701页

作者：李茜曹洁陈双朱长松刘韬陈兴东段满林徐建国南京大学医学院附属金陵医院汤山分院麻醉科江苏省南京211131 南京大学医学院附属金陵医院麻醉科江苏省南京211131

目的：探讨右美托咪定治疗全麻患者苏醒期躁动的有效性及安全性。方法选择 ASA I ～II级择期腹部手术患者120例，随机双盲分为右美托咪定组（A 组），咪达唑仑组（B 组）和0．9％氯化钠注射液对照组（C 组），每组40例。手术结束前40 mi... 详细信息

目的：探讨右美托咪定治疗全麻患者苏醒期躁动的有效性及安全性。方法选择 ASA I ～II级择期腹部手术患者120例，随机双盲分为右美托咪定组（A 组），咪达唑仑组（B 组）和0．9％氯化钠注射液对照组（C 组），每组40例。手术结束前40 min，A 组静脉泵注右美托咪定0．6μg／kg（10 min），B 组和 C 组分别静脉注射咪达唑仑30μg／kg 和0．9％氯化钠注射液1 mL，通过躁动评分、镇静评分、监测拔管前后的血压、心率和脉搏氧饱和度以及拔管时间等指标，评估患者在术后恢复室（PACU）的麻醉复苏情况。结果咪达唑仑组患者麻醉恢复时间[（18．2±1．9）min]、拔管时间[（32．1±3．9）min]和 PACU 停留时间[（48．7±3．1）min]明显长于右美托咪定组[（13．1±2．4）min、（26．5±2．2）min 和（39．8±3．4）min，P ＝0．023、0．040、0．003]和0．9％氯化钠注射液对照组[（12．6±2．3）min、（24．8±2．9）min 和（38．6±4．3）min，P ＝0．017、0．023、0．001）]；右美托咪定组和咪达唑仑组患者的镇静评分[（2．3±0．2）分和（2．4±0．1）分]明显优于0．9％氯化钠注射液对照组[（1．1±0．50）分，P ＝0．025、0．020]；右美托咪定组躁动情况[（1．3±0．5）分]明显优于咪达唑仑组和0．9％氯化钠注射液对照组[（2．5±0．5）分和（2．4±0．6）分，P ＝0．011、P ＝0．020]；在拔管前2 min、拔管即刻和拔管后5 min，右美托咪定组患者心率[（62．7±4．1）次／min、（67．3±3．4）次／min 和（63．2±4．3）次／min]均慢于咪唑安定组[（72．3±3．4）次／min、（84．9±5．3）次／min 和（82．1±3．1）次／min， P ＝0．002、0．001、0．001]和0．9％氯化钠注射液组[（73．6±2．9）次／min、（85．3±4．7）次／min 和（83．3±4．5）次／min，P ＝0．001、0．023、0．038）]；在拔管即刻，右美托咪定组患者平均动脉压[（87．3±4．2）mmHg）]低于咪达唑仑组[（93．1±4．3）mmHg，P ＝0．001]和0．9％氯化钠注射液对照组[（95．6±5．8）mmHg，P ＝0．001]，在拔管后5 min，右美托咪定组和咪达唑仑组患者平均动脉压[（84．5±3．1）mmHg]和[（85．1±2．9）mmHg]均低于0．9％氯化钠注射液对照组[（92．3±4．6）mmHg，P ＝0．023、0．038]。结论右美托咪定是治疗腹部手术患者全麻苏醒期躁动的理想药物，其疗效优于咪达唑仑。

关键词：右美托咪定麻醉,全身外科手术

FPGA-based Digital Implementation of Flexible Power Control for Three-phase to Single-phase MMC-based Advanced Co-phase Traction Power Supply System

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Journal of Modern Power Systems and Clean Energy 2023年第6期11卷 2015-2027页

作者： Jiangpeng Yang Hongjian Lin Haotian zhu Zeliang Shu Lan Ma changsong Cai Pengcheng Zhang Li He Ruoyu Li Shasha Song Josep M.Guerrero National Rail Transportation Electrification and Automation Engineering Technology Research Center Chengdu 611756China School of Electrical Engineering Southwest Jiaotong UniversityChengdu 611756China IEEE Department of Electrical Engineering City University of Hong KongHong KongChina School of Electrical Engineering and Automation Wuhan UniversityWuhan 430072China Department of Electrical Engineering Tsinghua UniversityBeijing 100084China Department of Energy Technology Aalborg UniversityAalborg 9220Denmark

A three-phase to single-phase modular multilevel converter based advanced co-phase traction power supply(MMC-ACTPS) system is an effective structure to address the concerns of phase splitting and poor power quality of the conventional electrified railway. Due to the large number of MMCACTPS system modules, I/O resources and computing speed have high requirements on processors. Moreover, the module capacitor balance is challenging because the sorting time is too long when the traditional sorting algorithm for voltage balance is used. To solve the above issues, a digital implementation scheme of flexible power control strategy for three-phase to single-phase MMC-ACTPS system based on field programmable gate array(FPGA), which has sufficient I/O resources, has been proposed. Due to the parallel execution characteristics of the FPGA, the execution time of the controller and the modulator can be greatly reduced compared with a digital signal processor(DSP) + FPGA or DSpace. In addition, an improved sorting algorithm is proposed to reduce the sorting time and the implementation steps are analyzed. Finally, simulation and experimental results are presented to demonstrate the effectiveness and correctness of the proposed control strategy.

关键词： Advanced co-phase power traction supply(ACTPS)system modular multilevel converter(MMC) digital implementation flexible power transmission circulating current mitigation

Rice CYP703A3, a cytochrome P450 hydroxylase, is essential for development of anther cuticle and pollen exine

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Journal of Integrative Plant Biology 2014年第10期56卷 979-994页

作者： Xijia Yang Di Wu Jianxin Shi Yi He Franck Pinot Bernard Grausem changsong Yin Lu zhu Mingjiao Chen Zhijing Luo Wanqi Liang Dabing Zhang State Key Laboratory of Hybrid Rice School of Life Sciences and Biotechnology Shanghai Jiao Tong University Institute of Plant Molecular Biology National Center for Scientific Research University of Strasbourg

Anther cuticle and pollen exine act as protective envelopes for the male gametophyte or pollen grain, but the mechanism underlying the synthesis of these lipidic polymers remains unclear. Previously, a tapetum‐expressed CYP703A3, a putative cytochrome P450 fatty acid hydroxylase, was shown to be essential for male fertility in rice（Oryza sativa L.）. However,the biochemical and biological roles of CYP703A3 has not been characterized. Here, we observed that cyp703a3‐2 caused by one base insertion in CYP703A3 displays defective pollen exine and anther epicuticular layer, which differs from Arabidopsis cyp703a2 in which only defective pollen exine occurs. Consistently, chemical composition assay showed that levels of cutin monomers and wax components were dramatically reduced in cyp703a3‐2 anthers. Unlike the wide range of substrates of Arabidopsis CYP703A2, CYP703A3 functions as an in‐chain hydroxylase only for a specific substrate, lauric acid, preferably generating 7‐hydroxylated lauric acid. Moreover, chromatin immunoprecipitation and expression analyses revealed that the expression of CYP703A3 is directly regulated by Tapetum Degeneration Retardation, a known regulator of tapetum PCD and pollen exine formation. Collectively, our results suggest that CYP703A3 represents a conserved and diversified biochemical pathway for in‐chain hydroxylation of lauric acid required for the development of male organ in higher plants.

关键词： Anther cuticle CYP703A3 cytochrome P450 hydroxylase 7‐hydroxylated lauric acid pollen exine

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卫城油田高油气比油井液力混抽增效工艺

石油钻探技术 2004年第1期32卷 60-62页

作者：张敬武杨顺贵刘长松祝明华吝拥军中原油田分公司采油工程事业部河南濮阳457001 中原油田分公司采油三厂河南濮阳457001

液力混抽增效的原理是通过控制井筒流体力学参数来调节油气井流动特性,尤其是流态,从而获得流动性良好的泡状流,达到提高抽汲效率、增加油气井产量、延长生产周期等目的。对于有杆泵采油井,该工艺实行三级优化系统。在不改变油井原来生... 详细信息

液力混抽增效的原理是通过控制井筒流体力学参数来调节油气井流动特性,尤其是流态,从而获得流动性良好的泡状流,达到提高抽汲效率、增加油气井产量、延长生产周期等目的。对于有杆泵采油井,该工艺实行三级优化系统。在不改变油井原来生产参数和作业方式的基础上,增效装置需要针对具体的油井依照专用模型进行设计。该增效装置满足所施工井的套管和油管尺寸等要求,所以安装容易、使用方便。在卫城油田WC2-67井等4口油井的现场试验结果表明,该工艺成功率100％,平均日增油1.3t,平均提高泵效14.4％。

关键词：液力混抽增效工艺油井井筒流体力学产量有杆泵油层压力

Palaeogeomorphology and its control on the development of sequence stratigraphy and depositional systems of the Early Silurian in the Tarim Basin

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Petroleum Science 2010年第3期7卷 311-322页

作者： Liu Jingyan Lin changsong Cai Zhenzhong zhu Yongfeng Yang Yongheng Peng Li Si Baoling Huang Zhen Li Huanpu Xu Yingcai Su Zhenzhen Key Lab of Marine Reservoir Evolution and Oil ＆ Gas Accumulation Mechanism of Ministry of Education China University ofGeosciences Beijing 100083 China Institute of Petroleum Exploration and Development Tarim Oilfield Company PetroChina Xinjiang 841000 China

The Silurian in the Tarim Basin was deposited on the basement deformed by the Caledonian tectonic movements at the end of the Late Ordovician. The development and distribution of sedimentary sequences of the Early Silurian have been clearly controlled by the palaeogeomorphology of the Late Ordovician. Based on unconformity characteristics and distribution of erosion, several zones can be differentiated including a high uplifted erosion zone, a transitional slope zone and a depression zone. The central and west Tabei Uplift zones show high angular unconformity and intense erosion. The Tarim Basin in the late Ordovician shows characteristics of higher in the west, lower in the east while higher in the south, lower in the north. The Early Silurian mainly developed transgressive and highstand systems tracts on the whole, while the lowstand systems tract only developed partly below the slope break. The palaeogeomorphology controlled the elastic source supply and deposit distribution. Braided delta system and tidal flat-estuary system were deposited. The duration of uplifting of the Tazhong paleo- uplift was longer than that of the Tabei paleo-uplift, and deposition was later. This led to the lower and middle members of the Kepingtage Formation missing in that area. As a large-scale transgression occurred during the deposition period of the upper member of the Kepingtage Formation, sediment from the west of the basin was transported and deposited by tides and waves, forming tidal-marine debris systems above the uplift. Proximal alluvial fan and fan delta coarse elastic deposits developed in proximal uplift zone in the east and southeast of the basin, and braided delta put forward to the transitional zone between the edge of uplift and the sea. Large-scale tidal channel, sub-distributary channel and mouth bar of the delta front can form favorable reservoirs, and they are primary targets for oil and gas exploration. This research on sequence-depositional systems development

关键词： Erosional palaeogeomorphology depositional palaeogeomorphology evolution of paleo- uplift sequence-depositional system favorable reservoir facies zone

一种基于离散空间矢量调制的Vienna整流器模型预测控制方法

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中国电机工程学报 2019年第20期39卷 6008-6016页

作者：朱文杰陈昌松段善旭强电磁工程与新技术国家重点实验室(华中科技大学)

应用于三相整流器的有限控制集-模型预测控制(finite control set model predictive control,FCS-MPC)方法以指标函数最小为目标,遍历计算输出单一最优电压矢量,开关频率不固定,输入电流品质依赖较高的采样频率。该文提出一种基于离散... 详细信息

应用于三相整流器的有限控制集-模型预测控制(finite control set model predictive control,FCS-MPC)方法以指标函数最小为目标,遍历计算输出单一最优电压矢量,开关频率不固定,输入电流品质依赖较高的采样频率。该文提出一种基于离散空间矢量调制(discretespacevector modulation,DSVM)的Vienna整流器模型预测控制方法。该方法采用由实矢量线性组合而成的虚拟矢量,在一个采样周期内可输出多个实矢量,能固定开关频率;通过引入虚拟矢量,增加预测控制中的可控矢量集,能有效减少参考电压和预测电压之间的误差,从而降低输入电流总谐波畸变率(total harmonics distortion,THD)。该文分析虚拟矢量及其调制方式,给出DSVM-MPC的实现方法。为验证所提DSVMMPC方法的正确性,与常规FCS-MPC方法进行仿真和实验对比分析,结果表明所提方法可提高输入电流品质。

关键词： Vienna整流器模型预测控制虚拟矢量离散空间矢量调制总电压误差