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Chinese Science Bulletin 2010年第11期55卷 1013-1019页

作者： LIU DanDan ZHANG JinChao yi changqing YANG MengSu Chemical Biology Laboratory College of Chemistry and Environmental Science Hebei University Baoding 071002 China Key Laboratory of Biochip Research Shenzhen Research Institute of City University of Hong Kong Shenzhen 518057 China Department of Biology and Chemistry City University ofHong Kong Hong Kong China

This study has investigated the effects of gold nanoparticles (Au NPs) on the proliferation, differentiation, and mineralization of a murine preosteoblast cell line MC3T3-E1 in vitro. The results show that Au NPs with diameters of both 20 and 40 nm promoted the proliferation, differentiation, and mineralization of MC3T3-E1 cells in a time- and dose-dependent manner at the concentra-tions of 1.5×10-5, 3.0×10-5, and 1.5×10-4 μmol/L. The reverse transcriptase polymerase chain reaction (RT-PCR) indicates that the expressions of runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), and osteocalcin (OCN) genes increased after the 20 and 40 nm Au NP treatments, and the expression levels were higher than those of the NaF group. The above results suggest that Au NPs have the potential to promote the osteogenic differentiation and miner- alization of MC3T3-E1 cells and the particle size plays a significant role in the process. Runx2, BMP-2, ALP, and OCN genes may interact with each other, further stimulating the osteogenic differentiation of MC3T3-E1 cells.

关键词：金纳米粒子细胞增殖成骨分化矿化体外核心结合因子α1 逆转录聚合酶链反应骨形态发生蛋白

An integrative review on the applications of 3D printing in the field of in vitro diagnostics

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Chinese Chemical Letters 2022年第5期33卷 2231-2242页

作者： Jian Yang Yanxiang Cheng Xia Gong Shengzhu yi Cheuk-Wing Li Lelun Jiang changqing yi Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringSun Yat-sen UniversityShenzhen 518107China Research Institute of Sun Yat-sen University in Shenzhen Shenzhen 518057China School of Science and Technology Nottingham Trent UniversityClifton LaneNottingham NG118NSUnited Kingdom

Biomedicine is one of the fastest growing areas of additive ***,in the field of in vitro diagnostics(IVD),contributions of 3D printing include i)rapid prototyping and iterative IVD proof-of-concept designing ranging from materials,devices to system integration;ii)conceptual design simpli-fication and improved practicality of IVD products;iii)shifting the IVD applications from centralized labs to point-of-care testing(POCT).In this review,the latest developments of 3D printing and its advantages in IVD applications are summarized.A series of 3D-printed objects for IVD applications,including single-function modules,multi-function devices which integrate several single-function modules for specific an-alytical applications such as sample pre-treatment and chemo-/bio-sensing,and all-in-one systems which integrate multi-function devices and the instrument operating them,are analyzed from the perspective of functional *** current and potential commercial applications of 3D-printed objects in the IVD field are *** features of 3D printing,especially rapid prototyping and low start-up,en-able the easy fabrication of bespoke modules,devices and systems for a range of analytical applications,and broadens the commercial IVD prospects.

关键词： 3D printing In vitro diagnostics Portable sensing device Point-of-care testing Stereolithography(SLA) Digital light projection(DLP) Fused deposition modeling(FDM)

Facile preparation of holmium(Ⅲ)-doped carbon nanodots for fluorescence/magnetic resonance dual-modal bioimaging

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Chinese Chemical Letters 2018年第8期29卷 1277-1280页

作者： Yaning Fang Jun Jia Jun Yang Junhui Zheng changqing yi Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province School of Engineering. Sun Yat-sen University Guangzhou 510006 China Guangdong General Hospital Guangzhou 510080 China Research Institute of Sun Yat-sen University in Shenzhen Shenzhen 618057 China

In this work, we report the synthesis of holmium（III）-doped carbon nanodots（Ho BCDs） as fluorescence/magnetic resonance（FL/MR） dual-modal imaging probes via a facile hydrothermal process using citrate acid（CA）, branched-polyethylenimine（BPEI） and diethylenetriamine pentaacetic acid hydrate holmium（III） dihydrogen salt（Ho-DTPA） as carbon source, passivating reagent and holmium source, *** thus prepared Ho BCDs exhibited ultra-small particle size（~4 nm）, high water solubility and bright fluorescence with an absolute quantum yield of 8%. Additionally, grey-scaled T_1-weighted images of Ho BCDs solution appeared to be apparently brighter than that of deionized water and un-doped blue carbon nanodots（BCDs） solution. In addition, in vitro toxicity assay validated superior biocompatibility of Ho BCDs. Using He La cells as models, Ho BCDs-treated cells were observed to emit blue fluorescence located both in plasma and nucleus, and presented positive contrast enhancement in T_1-weighted images, suggesting their potentials for practical biomedical applications.

关键词： Carbon dots Holmium Dual-modal imaging Fluorescence imaging Magnetic resonance imaging

Microwave-assisted synthesis of colorimetric and fluorometric dual-functional hybrid carbon nanodots for Fe^(3+) detection and bioimaging

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Chinese Chemical Letters 2021年第10期32卷 3189-3194页

作者： Yupeng Shi Jingjing Liu Yong Zhang Jianfeng Bao Jingliang Cheng changqing yi Key Laboratory of Functional Magnetic Resonance Imaging and Molecular Imaging(Henan Province) Department of MRIThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices School of Biomedical EngineeringSun Yat-sen UniversityShenzhen 518107China

Carbon nanodots(CDs) based fluorescent nanoprobes have recently drawn much attention in chemo-/bio-sensing and ***,it is still challenging to integrate the colorimetric and fluorometric dual readouts into a single ***,novel hybrid CDs(HCDs) are prepared by a simple microwave-assisted reaction of citric acid(CA),branched polyethyleneimine(BPEI) and potassium thiocyanate(KSCN).As-prepared HCDs show extraordinary properties,including excitation-dependent emission,satisfactory fluorescence quantum yield(46.8%),excellent biocompatibility and optical ***,the fluorescence intensity at 450 nm exhibits linear correlation over the Fe^(3+)concentration from 1 mmol/L to 150 mmol/L with a detection limit(LOD) of 52 nmol/***,the solution color changes from colorless to orange,and the absorbance at 460 nm increased linearly with Fe^(3+)concentration ranging from 0.02 mmol/L to 5 mmol/L(LOD:3.4 mmol/L).All the evidence illustrates that the HCDs can be conditioned for specific Fe^(3+)sensing with colorimetric and fluorometric dual readouts,which has also been verified with paper-based *** possible mechanism is attributed to the specific interactions between surface functional groups on the HCDs and Fe^(3+).Additionally,the HCDs are successfully applied in sensing Fe^(3+)in wastewater and living cells,demonstrating its potential applications in future environment monitoring and disease diagnosis.

关键词： Paper-based chip Carbon nanodots Colorimetric Fluorescence Fe^(3+)ions detection Dual-functional

Design of portable electrochemiluminescence sensing systems for point-of-care-testing applications

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Chinese Chemical Letters 2023年第5期34卷 88-103页

作者： Shuqi Xia Jiangfei Pan Deshen Dai Zong Dai Mengsu Yang changqing yi Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices School of Biomedical EngineeringSun Yat-sen UniversityShenzhen 518107China Research Institute of Sun Yat-sen University in Shenzhen Shenzhen 518057China Department of Biomedical Sciences&Tung Biomedical Sciences Centre City University of Hong KongHong KongChina

Point-of-care testing(POCT)technology is highly desirable for clinical diagnosis,healthcare monitoring,food safety inspection,and environment surveillance,because it enables rapid detection anywhere,anytime,and by ***(ECL)has been widely used in chemo-/bio analysis due to its advantages such as high sensitivity,simplicity,rapidity and easy to control,and is now attracting increasing attention for POCT ***,to realize the accurate on-site quantitation,it is still challenging to develop portable devices which can precisely collect,analyze,transmit and display the ECL *** review will focus on how to develop a portable ECL device by summarizing recent examples and analyzing their key components part by *** the possible solutions to the existing challenges in the development and applications of portable ECL devices are summarized and discussed in detail,followed by offering future *** attempted to provide an appealing viewpoint to inspire interested researchers to comprehend and explore portable ECL sensing systems for practical applications and even commercialization.

关键词： Smartphone Screen-printed electrode Bipolar electrode Machine learning Aggregation-induced ECL Nanoscale ECL luminophores Microfluidics

Facile synthesis and in vivo bioimaging applications of porphyrin derivative-encapsulated polymer nanoparticles

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Chinese Chemical Letters 2022年第8期33卷 4101-4106页

作者： Mengfei Hou Wandi Chen Junkai Zhao Deshen Dai Mo Yang changqing yi Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices School of Biomedical EngineeringSun Yat-sen UniversityShenzhen 518107China State Key Laboratory of Oncology in South China Collaborative Innovation Center For Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060China Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina

Fluorescence(FL)imaging guided photodynamic therapy(PDT)is becoming highly desirable for personalized therapy and precision *** this study,fluorescent polymer nanoparticles TCPP@PEI/PGA were facilely synthesized through electrostatic interaction-mediated self-assembly of porphyrins tetra(4-carboxyphenyl)porphine(TCPP)and polyethylenimine(PEI),and subsequent surface modification withγ-poly(glutamic acid)(γ-PGA).TCPP served a dual function as the FL imaging probe and the *** as-prepared TCPP@PEI/PGA nanoparticles showed excellent water-solubility and biocompatibility,while having outstanding capabilities of in vivo bioimaging and~1 O_(2) *** bioimaging of mice and effective killing of CT 26 cells as well as CT 26 tumor-bearing mice upon laser irradiation were successfully demonstrated when using TCPP@PEI/PGA as theranostic *** study provides a simple but robust method to design and synthesize porphyrin-based polymer nanoparticles for theranostics.

关键词： Porphyrins Fluorescence imaging Photodynamic therapy Theranostics

Nanotechnology for diagnosis and therapy of theumatoid arthritis:Evolution towards theranostic approaches

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Chinese Chemical Letters 2021年第1期32卷 66-86页

作者： Junkai Zhao Xuan Chen Kwun-Hei Ho Chao Cai Cheuk-Wing Li Mo Yang changqing yi Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringSun Yat-sen UniversityShenzhen 518107China Department of Biomedical Engineering the Hong Kong Polytechnic UniversityHong KongChina School of Science and Technology Nottingham Trent UniversityClifton LaneNottinghamNG118NSUnited Kingdom

Rheumatoid arthritis(RA),as a chronic autoimmune disease,damages the bone and cartilage of patients,and even leads to ***,the diagnosis and treatment of RA is particularly ***,due to the complexity of RA,it is difficult to make effective early diagnosis of RA,which is detrimental to RA ***,long-term intake of anti-RA drugs can also cause damage to patients' *** emergence of nanotechnology provides the new train of thoughts for the diagnosis and treatment of *** the combination of diagnosis and therapy is an ideal method to solve the problem of disease management of RA *** this review,we summarize the mechanism and microenvironment of RA,discuss the commonly used diagnostic techniques and therapeutic drugs for RA,and review their advantages and *** nanotherapy strategies such as drug-carrying nanoparticles,PTT,PDT are listed,and their applications in RA treatment are also *** addition,multimodal imaging,combined therapy and responsive diagnosis and treatment are also summarized as important *** last,we also review typical nanocarriers that can be used in the integration of diagnosis and therapy,and discussed their potential applications in RA theranostics.

关键词： Rheumatoid arthritis Diagnosis Therapy Theranostics Nanomedicine

A biomimetic upconversion nanoreactors for near-infrared driven H2 release to inhibit tauopathy in Alzheimer’s disease therapy

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Bioactive Materials 2024年第12期42卷 165-177页

作者： Qin Zhang Chuanqi Li Bohan yin Jiaxiang Yan Yutian Gu yingying Huang Jiareng Chen Xinyue Lao Jianhua Hao changqing yi yi Zhou James Chung Wai Cheung Siu Hong Dexter Wong Mo Yang School of Medicine and Pharmacy Ocean University of ChinaQingdao266003ChinaLaboratory for Marine Drugs and Bioproducts Qingdao Marine Science and Technology CenterQingdao266237China Department of Biomedical Engineering The Hong Kong Polytechnic University999077Hong KongChina The Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen518000China Research Center for Nanoscience and Nanotechnology The Hong Kong Polytechnic UniversityKowloon999077Hong KongChina Joint Research Center of Biosensing and Precision Theranostics The Hong Kong Polytechnic UniversityKowloon999077Hong KongChina Department of Applied Physics The Hong Kong Polytechnic University999077Hong KongChina Key Laboratory of Sensing Technology and Biomedical Instruments(Guangdong Province) School of Biomedical EngineeringSun Yat-Sen UniversityGuangzhou510000China Zhongshan School of Medicine Sun Yat-sen UniversityGuangzhou510080China

Abnormal hyperphosphorylation of tau protein is a principal pathological hallmark in the onset of neurodegenerative disorders,such as Alzheimer’s disease(AD),which can be induced by an excess of reactive oxygen species(ROS).As an antioxidant,hydrogen gas(H_(2))has the potential to mitigate AD by scavenging highly harmful ROS such as·***,conventional administration methods of H_(2) face significant challenges in controlling H_(2) release on demand and fail to achieve effective accumulation at lesion ***,we report artificial nanoreactors that mimic natural photosynthesis to realize near-infrared(NIR)light-driven photocatalytic H_(2) evolution in *** nanoreactors are constructed by biocompatible crosslinked vesicles(CVs)encapsulating ascorbic acid and two photosensitizers,chlorophyll a(Chla)and indoline dye(Ind).In addition,platinum nanoparticles(Pt NPs)serve as photocatalysts and upconversion nanoparticles(UCNP)act as light-harvesting antennas in the nanoreacting system,and both attach to the surface of *** NIR irradiation,the nanoreactors release H_(2) in situ to scavenge local excess ROS and attenuate tau hyperphosphorylation in the AD mice *** NIR-triggered nanoreactors provide a proof-of-concept design for the great potential of hydrogen therapy against AD.

关键词： Artificial photosynthesis Hydrogen therapy Oxidative stress Tau hyperphosphorylation Alzheimer’s disease

An AIEgen/graphene oxide nanocomposite(AIEgen@GO)-based two-stage“turn-on”nucleic acid biosensor for rapid detection of SARS-CoV-2 viral sequence

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Aggregate 2023年第1期4卷 219-230页

作者： Qin Zhang Bohan yin Jianhua Hao Linjie Ma yingying Huang Xueying Shao Chuanqi Li Zhiqin Chu changqing yi Siu Hong Dexter Wong Mo Yang Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina Department of Applied Physics The Hong Kong Polytechnic UniversityHong KongChina Department of Electrical and Electronic Engineering Joint Appointment with School of Biomedical SciencesThe University of Hong KongHong KongChina Key Laboratory of Sensing Technology and Biomedical Instruments(Guangdong Province) School of Biomedical EngineeringSun Yat-Sen UniversityGuangzhouPR China

The ongoing outbreak of Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)pandemic has posed significant challenges in early viral ***,it is urgently desirable to develop a rapid,inexpensive,and sensitive method to aid point-of-care SARS-CoV-2 *** this work,we report a highly sequence-specific biosensor based on nanocomposites with aggregationinduced emission luminogens(AIEgen)-labeled oligonucleotide probes on graphene oxide nanosheets(AIEgen@GO)for one step-detection of SARS-CoV-2-specific nucleic acid sequences(Orf1ab or N genes).A dual“turn-on”mechanism based on AIEgen@GO was established for viral nucleic acids ***,the first-stage fluorescence recovery was due to dissociation of the AIEgen from GO surface in the presence of target viral nucleic acid,and the second-stage enhancement of AIEbased fluorescent signal was due to the formation of a nucleic acid duplex to restrict the intramolecular rotation of the ***,the feasibility of our platform for diagnostic application was demonstrated by detecting SARS-CoV-2 virus plasmids containing both Orf1ab and N genes with rapid detection around 1 h and good sensitivity at pM level without *** platform shows great promise in assisting the initial rapid detection of the SARS-CoV-2 nucleic acid sequence before utilizing quantitative reverse transcription-polymerase chain reaction for second confirmation.

关键词： aggregation-induced emission(AIE)luminogen graphene oxide SARS-CoV-2 detection

Development of Hybrid Nanomaterial-based Biomedical Sensors

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Development of Hybrid Nanomaterial-based Biomedical Sensors