检索结果-南通市图书馆

Beverage Plant Research 2023年第1期3卷 137-150页

作者： changqing Ding Xinyuan hao Lu Wang Nana Li Jianyan Huang Jianming Zeng Yajun Yang Xinchao Wang National Center for Tea Plant Improvement Key Laboratory of BiologyGenetics and Breeding of Special Economic Animals and PlantsMinistry of Agriculture and Rural AffairsTea Research InstituteChinese Academy of Agricultural SciencesHangzhou 310008PR China

To gain a better understanding on the mechanism of cold acclimation in tea plant[Camellia sinensis(L.)***]at the proteome level,an iTRAQ based quantitative proteome analysis was carried out to identify differentially accumulated proteins in the mature leaves which were collected at non-acclimated(NA),fully acclimated(CA)and de-acclimated(DA)stages.407 and 477 proteins identified from CA and DA showed significant abundance changes(at 95%confidence)compared with NA,***,251 protein species changed their abundance in DA compared with *** differential abundance protein species were mainly involved in metabolism,cell wall,photosynthesis,energy,protein synthesis,antioxidation,carbohydrate metabolic process and binding,and mapped to the pathways of biosynthesis of secondary metabolites,microbial metabolism in diverse environment,ribosome,metabolic pathway,sugar metabolism,protein processing,photosynthesis,and plant-pathogen interaction ***,no significant correlation was detected between the identified proteins and cognate gene transcript levels by correlation analysis and qRT-PCR *** study presents a comprehensive proteome in mature leaves at different cold acclimation status and provides new insights into cold acclimation mechanisms in tea plants.

关键词： metabolism analysis sugar

An AIEgen/graphene oxide nanocomposite(AIEgen@GO)-based two-stage“turn-on”nucleic acid biosensor for rapid detection of SARS-CoV-2 viral sequence

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aggregate

Aggregate 2023年第1期4卷 219-230页

作者： Qin Zhang Bohan Yin Jianhua hao Linjie Ma Yingying Huang Xueying Shao Chuanqi Li Zhiqin Chu changqing Yi Siu Hong Dexter Wong Mo Yang Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina Department of Applied Physics The Hong Kong Polytechnic UniversityHong KongChina Department of Electrical and Electronic Engineering Joint Appointment with School of Biomedical SciencesThe University of Hong KongHong KongChina Key Laboratory of Sensing Technology and Biomedical Instruments(Guangdong Province) School of Biomedical EngineeringSun Yat-Sen UniversityGuangzhouPR China

The ongoing outbreak of Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)pandemic has posed significant challenges in early viral ***,it is urgently desirable to develop a rapid,inexpensive,and sensitive method to aid point-of-care SARS-CoV-2 *** this work,we report a highly sequence-specific biosensor based on nanocomposites with aggregationinduced emission luminogens(AIEgen)-labeled oligonucleotide probes on graphene oxide nanosheets(AIEgen@GO)for one step-detection of SARS-CoV-2-specific nucleic acid sequences(Orf1ab or N genes).A dual“turn-on”mechanism based on AIEgen@GO was established for viral nucleic acids ***,the first-stage fluorescence recovery was due to dissociation of the AIEgen from GO surface in the presence of target viral nucleic acid,and the second-stage enhancement of AIEbased fluorescent signal was due to the formation of a nucleic acid duplex to restrict the intramolecular rotation of the ***,the feasibility of our platform for diagnostic application was demonstrated by detecting SARS-CoV-2 virus plasmids containing both Orf1ab and N genes with rapid detection around 1 h and good sensitivity at pM level without *** platform shows great promise in assisting the initial rapid detection of the SARS-CoV-2 nucleic acid sequence before utilizing quantitative reverse transcription-polymerase chain reaction for second confirmation.

关键词： aggregation-induced emission(AIE)luminogen graphene oxide SARS-CoV-2 detection

Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 3413-3424页

作者： Ruimin Zhang Jingjing Chang Jiayue Li Guangpu Lan changqing Xuan hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Shujuan Tian Li Yuan Xian Zhang Chunhua Wei State Key Laboratory of Crop Stress Biology for Arid Areas College of HorticultureNorthwest A&F UniversityYangling 712100 ShaanxiChina State Key Laboratory of Vegetable Germplasm Innovation Tianjin Kernel Vegetable Research InstituteTianjin 300384China

Although male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production,its underlying molecular mechanisms in Cucurbitaceae species are still largely ***,a spontaneous male-sterile watermelon mutant,Se18,was reported to have abnormal tapetum development,which resulted in completely aborted pollen ***-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1(ClATM1)encodes a basic helix-loop-helix(bHLH)transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional(BIF)domain in Se18 ***–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther *** genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated ***,ClATM1 was significantly downregulated in the Se18 mutant,displaying a clear dose effect at the transcriptional *** dual-luciferase reporter,β-glucuronidase(GUS)activity,and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter ***,ClATM1 is the first male sterility gene cloned from watermelon,and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants.

关键词： bHLH sterility sterile

Biomimetic asymmetric catalysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2023年第6期66卷 1553-1633页

作者： Xiao Xiao Kaini Xu Zhong-Hua Gao Zhou-hao Zhu changqing Ye Baoguo Zhao Sanzhong Luo Song Ye Yong-Gui Zhou Senmiao Xu Shou-Fei Zhu Hongli Bao Wei Sun Xiaoming Wang Kuiling Ding State Key Laboratory of Organometallic Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China The Education Ministry Key Laboratory of Resource Chemistry Shanghai Key Laboratory of Rare Earth Functional Materialsand Shanghai Frontiers Science Center of Biomimetic CatalysisShanghai Normal UniversityShanghai 200234China Center of Basic Molecular Science Department of ChemistryTsinghua UniversityBeijing 100084China Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Molecular Recognition and FunctionInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China State Key Laboratory and Institute of Elemento-Organic Chemistry College of ChemistryNankai UniversityTianjin 300071China Key Laboratory of Coal to Ethylene Glycol and Its Related Technology State Key Laboratory of Structural ChemistryCenter for Excellence in Molecular SynthesisFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

Enzymes are the core for biological transformations in *** structures and functions have drawn enormous attention from biologists as well as chemists since last *** large demand of bioactive molecules and the pursuit of efficiency and greenness of synthesis have spurred the rapid development of biomimetic chemistry in the past several *** asymmetric catalysis,mimicking the structures and functions of enzymes,has been recognized as one of the most promising synthetic strategies for the synthesis of valuable chiral *** review summarizes the evolution of asymmetric catalysis inspired by aldolases,vitamin B_(1)/B_(6)-dependent enzymes,NAD(P)H,flavin,hydrogenases,heme oxygenases,nonheme oxygenases,and dinuclear/multinuclear metalloenzymes in aspects of biomimetic design,catalyst development and related catalytic *** well-established synthetic approaches originating from biological reactions have demonstrated the unique prowess of biomimetic asymmetric catalysis in bridging the gap between bio-catalysis and chemical synthesis.

关键词： biomimetic catalysis amine catalysis carbene catalysis vitamin Br carbonyl catalysis vitamin Bo NAD(P)H flavin biomimetic oxidation dinuclear/multinuclear metal catalysis asymmetric hydrogenation

中国上消化道癌高发区非甾体类抗炎药使用情况研究

在线全文

学校读者我要写书评

暂无评论

中华预防医学杂志 2019年第11期53卷 1098-1103页

作者：谢双华陈茹赵德利刘玉琴郝长青张永贞宋国慧华召来王家林刘曙正张立玮邵丹彤秦宇李敏娟周家琛郑荣寿王贵齐魏文强国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院肿瘤登记办公室北京100021 肥城市人民医院肿瘤防治中心 271600 甘肃省医学科学研究院肿瘤流行病学研究中心兰州730030 林州市肿瘤医院林州市肿瘤防治研究所腔镜室 456500 山西省肿瘤医院流行病室太原030000 磁县肿瘤医院/肿瘤防治研究所流行病学研究室 056500 扬中市人民医院/扬中市肿瘤防治研究所 212200 山东大学附属山东省肿瘤医院科教部济南250117 河南省肿瘤医院疾病预防控制科郑州450000 河北医科大学第四医院内镜中心石家庄050019 国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院内镜科北京100021

目的分析中国部分上消化道癌高发区非甾体类抗炎药(NSAIDs)的使用现况.方法基于"中国食管癌专病队列"和"消化道恶性肿瘤(食管癌、胃癌、大肠癌)高危人群识别及高危人群预防研究",于2017年1月至2018年8月,在6个省份的12个上消化道癌高... 详细信息

目的分析中国部分上消化道癌高发区非甾体类抗炎药(NSAIDs)的使用现况.方法基于"中国食管癌专病队列"和"消化道恶性肿瘤(食管癌、胃癌、大肠癌)高危人群识别及高危人群预防研究",于2017年1月至2018年8月,在6个省份的12个上消化道癌高发地区,选取212个食管癌、胃癌高发的乡村/社区,招募35 910名符合纳入标准的40~69岁居民为研究对象.通过问卷调查和体检收集非甾体类药物的使用情况、社会人口学特征、健康相关行为等相关信息,并采用χ2检验和Cochran-Armitage趋势检验分析NSAIDs的使用情况及其性别、年龄和地区间的差异.结果研究对象年龄为(54.6±7.1)岁,男性占43.42%(15 591名);NSAIDs使用率为4.56%(1 638名).不同省份的NSAIDs的使用率差异具有统计学意义(P<0.001);女性使用率[4.87%(1 750名)]高于男性[4.24% (1 524名)](P<0.001);随着年龄增长,NSAIDs使用率呈增加趋势(P趋势<0.001);随着NSAIDs使用频率增加,胃肠道症状、胃肠道溃疡和黑便发生率均呈增加趋势(P趋势值均<0.05).结论中国部分上消化道癌高发区NSAIDs的使用较为普遍;随着NSAIDs使用频率增加可能会导致胃肠道相关的不良反应.

关键词：消炎药,非甾类横断面研究使用情况

A biomimetic upconversion nanoreactors for near-infrared driven H2 release to inhibit tauopathy in Alzheimer’s disease therapy

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2024年第12期42卷 165-177页

作者： Qin Zhang Chuanqi Li Bohan Yin Jiaxiang Yan Yutian Gu Yingying Huang Jiareng Chen Xinyue Lao Jianhua hao changqing Yi Yi Zhou James Chung Wai Cheung Siu Hong Dexter Wong Mo Yang School of Medicine and Pharmacy Ocean University of ChinaQingdao266003ChinaLaboratory for Marine Drugs and Bioproducts Qingdao Marine Science and Technology CenterQingdao266237China Department of Biomedical Engineering The Hong Kong Polytechnic University999077Hong KongChina The Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen518000China Research Center for Nanoscience and Nanotechnology The Hong Kong Polytechnic UniversityKowloon999077Hong KongChina Joint Research Center of Biosensing and Precision Theranostics The Hong Kong Polytechnic UniversityKowloon999077Hong KongChina Department of Applied Physics The Hong Kong Polytechnic University999077Hong KongChina Key Laboratory of Sensing Technology and Biomedical Instruments(Guangdong Province) School of Biomedical EngineeringSun Yat-Sen UniversityGuangzhou510000China Zhongshan School of Medicine Sun Yat-sen UniversityGuangzhou510080China

Abnormal hyperphosphorylation of tau protein is a principal pathological hallmark in the onset of neurodegenerative disorders,such as Alzheimer’s disease(AD),which can be induced by an excess of reactive oxygen species(ROS).As an antioxidant,hydrogen gas(H_(2))has the potential to mitigate AD by scavenging highly harmful ROS such as·***,conventional administration methods of H_(2) face significant challenges in controlling H_(2) release on demand and fail to achieve effective accumulation at lesion ***,we report artificial nanoreactors that mimic natural photosynthesis to realize near-infrared(NIR)light-driven photocatalytic H_(2) evolution in *** nanoreactors are constructed by biocompatible crosslinked vesicles(CVs)encapsulating ascorbic acid and two photosensitizers,chlorophyll a(Chla)and indoline dye(Ind).In addition,platinum nanoparticles(Pt NPs)serve as photocatalysts and upconversion nanoparticles(UCNP)act as light-harvesting antennas in the nanoreacting system,and both attach to the surface of *** NIR irradiation,the nanoreactors release H_(2) in situ to scavenge local excess ROS and attenuate tau hyperphosphorylation in the AD mice *** NIR-triggered nanoreactors provide a proof-of-concept design for the great potential of hydrogen therapy against AD.

关键词： Artificial photosynthesis Hydrogen therapy Oxidative stress Tau hyperphosphorylation Alzheimer’s disease

杂交变异果蝇优化算法在冗余度机械手逆运动的应用研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

机械科学与技术 2022年第5期41卷 747-754页

作者：刘浩姜长青衡水学院物理与电子信息系机电研究所河北衡水053000 清华大学航天航空学院北京100084

逆运动学问题是冗余度机器人运动控制、轨迹规划和动力学分析的基础,也是机器人学中最重要的问题之一。针对末端执行器位姿的最小误差为优化目标,建立了适应度函数,将冗余度机械手的逆运动学问题转化为一个等价的优化问题,在种群智能优... 详细信息

逆运动学问题是冗余度机器人运动控制、轨迹规划和动力学分析的基础,也是机器人学中最重要的问题之一。针对末端执行器位姿的最小误差为优化目标,建立了适应度函数,将冗余度机械手的逆运动学问题转化为一个等价的优化问题,在种群智能优化算法基础上应用了杂交变异果蝇优化算法(HMFOA)进行冗余度机械手逆运动学问题的求解。采用嗅觉搜索杂交突变机制和视觉搜索的动态实时更新机制,能有效地解决果蝇优化算法(FOA)的收敛问题,并提高算法的收敛速度。为进一步验证HMFOA的有效性,在七自由度机械手上对HMFOA进行了测试,对其结果与FOA、LGMS-FOA和AE-LGMS-FOA等算法进行了比较,实验结果表明HMFOA能有效地解决冗余机械手的逆运动学问题。

关键词：冗余度机械手逆运动学杂交变异果蝇算法

在线全文

学校读者我要写书评

暂无评论

南方冬季湿雪的光谱特征分析

冰川冻土 2015年第6期37卷 1515-1523页

作者：钱昊柯长青江苏省地理信息技术重点实验室/南京大学地理与海洋科学学院江苏南京210023

使用Field Spec4便携式地物光谱仪获取了南京地区冬季湿雪的光谱数据,并从太阳高度角、坡度、坡向、下垫面及混合雪几个方面进行了分析.结果表明:积雪的反射率在可见光区变化平稳,在近红外区下降迅速,1 020 nm、1 250 nm附近是积雪反射... 详细信息

使用Field Spec4便携式地物光谱仪获取了南京地区冬季湿雪的光谱数据,并从太阳高度角、坡度、坡向、下垫面及混合雪几个方面进行了分析.结果表明:积雪的反射率在可见光区变化平稳,在近红外区下降迅速,1 020 nm、1 250 nm附近是积雪反射率的吸收区;湿雪状态下,太阳高度角增大的同时积雪反射率逐渐降低,而在水湿雪状态下太阳高度角对反射率影响减弱,积雪含水率成为其变化的主要影响因素;积雪反射率随着坡度的增大而增大;积雪在朝阳坡的反射率远高于背阴坡,背阴坡积雪反射率在可见光谱区受影响较大;当积雪厚度达到一定值后下垫面的差异对反射率影响有限,否则会对反射率产生较大影响;在可见光波段纯雪反射率最高,近红外波段植被/积雪混合反射率最高,林下雪的光谱反射率较低.南京地区湿雪光谱测量和分析对了解南方湿雪光谱特性和变化规律,以及对于积雪定量遥感及其参数反演具有重要意义.

关键词：南方冬季湿雪光谱测量光谱分析光谱特征

在线全文

学校读者我要写书评

暂无评论

电子稳像算法的速度与精度改进

计算机辅助设计与图形学学报 2018年第8期30卷 1543-1553页

作者：董常青程雪岷郝群清华大学深圳研究生院深圳518055 北京理工大学光电学院北京100081

电子稳像算法的目的是实时地去除视频拍摄过程中造成的随机不规则运动,以便后端的图像处理操作.针对处理时间以及精度方面进行研究提出了一种电子稳像算法.首先,实现了特征点个数的自适应提取,然后,提出并应用了满足凸优化条件的三点求... 详细信息

电子稳像算法的目的是实时地去除视频拍摄过程中造成的随机不规则运动,以便后端的图像处理操作.针对处理时间以及精度方面进行研究提出了一种电子稳像算法.首先,实现了特征点个数的自适应提取,然后,提出并应用了满足凸优化条件的三点求解和随机点校正来优化仿射矩阵,再对计算过程各阶段误差进行了优化控制,最后,通过局部提取运动目标区域进行动态补偿,将计算时间从20 ms减少到1 ms.实验结果表明,该算法每帧处理时间约为7 ms(不包括特征点提取部分),并具有较好的稳像效果.

关键词：电子稳像仿射矩阵全局补偿自适应阈值

Food-Borne Poisoning Accident from Amanitin Toxin in Wild Mushrooms—Xingtai City,Hebei Province,China,2023

在线全文

学校读者我要写书评

暂无评论

China CDC weekly 2024年第3期6卷 56-59页

作者： Baopu Lv Liang Liu hao Xiao Qingbing Meng Rui Zhang Yaqing An Yingli Jin Yu Ma Hengbo Gao Yongkai Li Qian He Yutao Zhang changqing Liu Xiaoyan Luo Xiaomin Xu Fenshuang Zheng Yingping Tian Hongshun Zhang Dongqi Yao Emergency Department The Second Hospital of Hebei Medical UniversityShijiazhuang CityHebei ProvinceChina National Institute of Occupational Health and Poison Control Chinese Centre for Disease Control and PreventionBeijingChina Hebei Provincial Center for Disease Control and Prevention Shijiazhuang CityHebei ProvinceChina Zhejiang Provincial Center for Disease Control and Prevention Hangzhou CityZhejiang ProvinceChina Emergency Department The Affiliated Hospital of Yunnan UniversityKunming CityYunnan ProvinceChina

What is already known about this topic?Fatal poisonings caused by wild mushrooms containing amanita toxins pose a significant threat in the southern regions of *** toxins primarily induce gastrointestinal symptoms initially,which are then followed by potentially life-threatening acute liver *** is added by this report?This report contributes to the existing knowledge on these cases of poisoning by documenting the second occurrences in Hebei Province and the first occurrences in Xingtai *** individuals reported consuming wild mushrooms from the same origin,and laboratory tests confirmed the presence ofα-amanitin in their blood *** are the implications for public health practice?This underscores the risk associated with the collection and consumption of amanita toxin-containing mushrooms in *** is important to note that the identification of toxic and non-toxic mushrooms should not solely rely on personal experience or appearance.

关键词： Hebei consuming initially