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Reduced clinical severity during 2022 Shanghai Spring epidemic of SARS-CoV-2 omicron BA.2 variant infection——an integrated account of virus pathogenicity and vaccination effectiveness

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National Science Review 2024年第4期11卷 24-27页

作者： Xingyue Wu Yao Chen Kangli Cao Yao Shen Xueling Wu Yilin yang Zhongshu Kuang Qingrun li Zhenzhen lu Yichen Jia mian Shao Guorong Gu Xiangwei wang Ye Yao Ying wang Shaodie Chen Zhigao Yu Wei Wei longfei Ding lulu lan Tianwen Gu Xiangyu long Jian Sun lingyu Xing Jiayuan Shen Yi Han Yue luo Sucheng Mu Mengna lin Xiaoyan zhang Rong Zeng Jianqing Xu Guoping Zhao lihong Huang Zhenju Song Department of Emergency Medicine Clinical Center for Bio-Therapy Department of Biostatistics and Department of UrologyShanghai Key Laboratory of Lung Inflammation and Injury Zhongshan Hospital Fudan University Department of Respiratory and Critical Care Medicine Shanghai Pudong Hospital Fudan University Department of Pulmonology Renji Hospital Shanghai Jiao Tong University School of Medicine Key laboratory of Systems Biology Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai Public Health Clinical Center Fudan University Department of Biostatistics School of Public Health Key Laboratory of Public Health Safety of Ministry of Education and Shanghai Institute of Infectious Disease and Biosecurity School of Public Health Fudan University Shanghai Institute of Immunology Department of Immunology and Microbiology Shanghai Institute of Virology Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of EducationSchool of Medicine Shanghai Jiao Tong University State Key laboratory of Genetic Engineering Fudan Microbiome CenterSchool of Life Sciences Fudan University Bio-Med Big Data Center Shanghai Institute of Nutrition and Health Chinese Academy of Sciences

The emergence of SARS-Co V-2 variant of concern omicron BA.2 led to high infection incidence rates as well as reduced severity of COVID-19 during the Shanghai Spring epidemic of 2022 [1]. We assessed the role of viral pathogenicity and the effectiveness of inactivated vaccines in a retrospective observational study including 10 258 confirmed COVID-19cases identified between April and June of 2022, from four COVID-19 designated hospitals located in two areas of Shanghai, namely the West Bund and the East Bund, where the populations’ demographic characteristics varied significantly (Table S1, Fig. S1).

关键词： REPlICATION

Spring constant regulate selectin-ligand bond dissociation

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医用生物力学 2013年第S1期28卷 95-96页

作者： yan zhang Ganyun Sun shouqin l mian long Center of Biomechanics and Bioengineering and Key laboratory of Microgravity Institute of MechanicsChinese Academy of Sciences

Forced dissociation of selectin-ligand complex is crucial to such biological processes as leukocyte recruitment,thrombosis formation,as well as tumor metastasis[1].Although several assays and techniques,e.g.,dynamic force spectroscopy（DFS）,have been applied to probe the complex at single-bond level,the discrepancies in the loading rate dependence of bond rupture force were found in the assays,presumably due to the different pathways in energy landscape and binding kinetics of molecular complexes[2].However,the underlying mechanisms remain *** an optical trap（OT）assay was used to quantify the bond rupture at rf≤20 pN/s

关键词： selectin dissociation rupture kinetics landscape metastasis regulate quantify leukocyte unclear

Multi-scale molecular dynamics simulations and applications on mechanosensitive proteins of integrins

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Chinese Physics B 2021年第3期30卷 1-8页

作者： shouqin lu Qihan Ding Mingkun zhang mian long Center of Biomechanics and Bioengineering Key Laboratory of Microgravity(National Microgravity Laboratory)Beijing Key Laboratory of Engineered Construction and Mechanobiologyand CAS Center for Excellence in Complex System MechanicsInstitute of MechanicsChinese Academy of Sciences(CAS)Beijing 100190China School of Engineering Science University of Chinese Academy of SciencesBeijing 101408China Chongqing Engineering Research Center of High-Resolution and 3D Dynamic Imaging Technology Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China

Molecular dynamics simulation(MDS)is a powerful technology for investigating evolution dynamics of target proteins,and it is used widely in various fields from materials to *** mini-review introduced the principles,main preforming procedures,and advances of MDS,as well as its applications on the studies of conformational and allosteric dynamics of proteins especially on that of the mechanosensitive *** perspectives were also *** review could provide clues in understanding the potentiality of MD simulations in structure–function relationship investigation of biological proteins.

关键词： molecular dynamics simulations mechanosensitive protein allosteric dynamics integrin

Kinetics of Receptor-ligand Interactions in Immune Responses

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Cellular & Molecular Immunology 2006年第2期3卷 79-86页

作者： mian long shouqin lue Ganyun Sun National Microgravity laboratory and Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China Corresponding to： Dr. mian long National Microgravity Laboratory and Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China

Receptor-ligand interactions in blood flow are crucial to initiate the biological processes as inflammatory cascade, platelet thrombosis, as well as tumor metastasis. To mediate cell adhesions, the interacting receptors and ligands must be anchored onto two apposing surfaces of two cells or a cell and a substratum, i.e., the two-dimensional （2D） binding, which is different from the binding of a soluble ligand in fluid phase to a receptor, i.e., （3D） binding. While numerous works have been focused on 3D kinetics of receptor-ligand interactions in immune systems, 2D kinetics and its regulations have less been understood, since no theoretical framework and experimental assays have been established until 1993. Not only does the molecular structure dominate 2D binding kinetics, but the shear force in blood flow also regulates cell adhesions mediated by interacting receptors and iigands. Here we provided the overview of current progresses in 2D bindings and regulations. Relevant issues of theoretical frameworks, experimental measurements, kinetic rates and binding affinities, and force regulations, were discussed.

关键词： kinetics force receptor-ligand bond cell adhesion

Simulation and prediction of membrane fusion dynamics

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Theoretical & Applied Mechanics letters 2022年第1期12卷 49-55页

作者： Zhi Zheng shouqin lü mian long Center of Biomechanics and Bioengineering Key Laboratory of Microgravity(National Microgravity Laboratory)Beijing Key Laboratory of Engineered Construction and Mechanobiologyand CAS Center for Excellence in Complex System MechanicsInstitute of MechanicsChinese Academy of SciencesBeijing 100190China School of Engineering Science University of Chinese Academy of SciencesBeijing 100049China

Membrane fusion is an important process by which biological membranes perform their life activities. Simulations show that the membrane fusion process happens mainly through three pathways, where the Stalk-Pore hypothesis, in which two membranes come into close contact to form a stalk to a hemifusion intermediate, and then the fusion pore opens to achieve completely fusion, is widely accepted, and there exist two free energy barriers that break the current structural steady state for lipid rearrangement. Factors of lipid composition, mechanical environment, protein and ion have regulatory roles in the membrane fusion process by effecting membrane curvature structurally and the free energy barriers from energetic perspective. Meanwhile, many theoretical models, represented by the Helfrich model, have been proposed to predict the membrane fusion process. In this paper, we review the research process of membrane fusion and mainly introduce the dynamics of membrane fusion, regulation factors and typical theoretical models.

关键词： Membrane fusion Hemifusion intermediates fusion pathway Regulation Helfrich model

Mechanotransduction of liver sinusoidal endothelial cells under varied mechanical stimuli

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Acta Mechanica Sinica 2021年第2期37卷 201-217页

作者： Xinyu Shu Ning li Yi Wu wang li Xiaoyu zhang Peiwen li Dongyuan lu shouqin lu mian long Center for Biomechanics and Bioengineering Key Laboratory of Microgravity(National Microgravity Laboratory)and Beijing Key Laboratory of Engineered Construction and MechanobiologyInstitute of MechanicsChinese Academy of SciencesBeijing 100190China School of Engineering Sciences University of Chinese Academy of SciencesBeijing 100049China

liver sinusoidal endothelial cells(lSECs)are the gatekeeper of liver to maintain hepatic *** are formed into the highly specialized endothelium between vascular lumen and the space of Disse and are mechanosensitive to respond varied *** stress and mechanical stretch induced by blood perfusion and substrate stiffness enhancement derived from deposition of extracellular matrix(ECM)are major mechanical stimuli that surround *** review introduces how lSECs respond to the external forces in both physiological and pathological cases and what is the interplay of lSECs with other hepatic *** mechanisms that potentiate lSECs mechanotransduction are also discussed.

关键词： liver sinusoidal endothelial cell Shear stress Mechanical stretch Extracellular matrix Stiffness

Associations of sleeping patterns and isotemporal substitution of other behavior with the prevalence of CKD in Chinese adults

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Frontiers of Medicine 2024年第2期18卷 303-314页

作者： Yi Ding Xiaoli Xu Zhuojun Xin Qiuyu Cao Jiaojiao Huang Xianglin Wu yanan Huo Qin Wan Yingfen Qin Ruying Hu lixin Shi Qing Su Xuefeng Yu li yan Guijun Qin Xulei Tang Gang Chen Min Xu Tiange wang Zhiyun Zhao Zhengnan Gao Guixia wang Feixia Shen Zuojie luo li Chen Qiang li Zhen Ye Yinfei zhang Chao liu Youmin wang Tao yang Huacong Deng lulu Chen Tianshu Zeng Jiajun Zhao Yiming Mu Shengli Wu Yuhong Chen Jieli lu Weiqing wang Guang Ning Yu Xu Yufang Bi mian li Department of Endocrine and Metabolic Diseases Shanghai Institute of Endocrine and Metabolic DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai200025China Shanghai National Clinical Research Center for Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of ChinaShanghai Key Laboratory for Endocrine TumorState Key Laboratory of Medical GenomicsRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai200025China Jiangxi Provincial People’s Hospital Affiliated to Nanchang UniversityNanchang330000China Department of Endocrinology The Affiliated Hospital of Southwest Medical UniversityLuzhou646000China Department of Endocrinology The First Affiliated Hospital of Guangxi Medical UniversityNanning530000China Zhejiang Provincial Center for Disease Control and Prevention Hangzhou310000China Department of Endocrinology Affiliated Hospital of Guiyang Medical UniversityGuiyang550000China Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200000China tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430000China Sun Yat-sen Memorial Hospital Sun Yat-sen UniversityGuangzhou510000China Department of Endocrinology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou450000China Department of Endocrinology The First Hospital of Lanzhou UniversityLanzhou730000China fujian Provincial Hospital Fujian Medical UniversityFuzhou350000China Dalian Municipal Central Hospital Affiliated of Dalian Medical UniversityDalian116000China Department of Endocrinology The First Hospital of Jilin UniversityChangchun130000China Department of Endocrinology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou325000China Qilu Hospital of Shandong University Jinan250000China Department of Endocrinology The Second Affiliated Hospital of Harbin Medical UniversityHarbin150000China Department of Endocrinology Cen

Studies have found a U-shaped relationship between sleep duration and chronic kidney disease(CKD)risk,but limited research evaluated the association of reallocating excessive sleep to other behavior with *** included 104538 participants from the nationwide cohort of the Risk Evaluation of Cancers in Chinese Diabetic Individuals:A longitudinal Study,with self-reported time of daily-life *** isotemporal substitution models,we found that substituting 1 h of sleeping with sitting,walking,or moderate-to-vigorous physical activity was associated with a lower CKD ***-time physical activity displacement was associated with a greater prevalence reduction than occupational physical activity in working *** stratified analysis,a lower CKD prevalence related to substitution toward physical activity was found in long *** pronounced correlations were observed in long sleepers with diabetes than in those with prediabetes,and they benefited from other behavior substitutions toward a more active *** U-shaped association between sleep duration and CKD prevalence implied the potential effects of insufficient and excessive sleep on the kidneys,in which the pernicious link with oversleep could be reversed by time reallocation to physical *** divergence in the predicted effect on CKD following time reallocation to behavior of different domains and intensities and in subpopulations with diverse metabolic statuses underlined the importance of optimizing sleeping patterns and adjusting integral behavioral composition.

关键词： sleep physical activity chronic kidney disease isotemporal substitution behavioral pattern

The impact of N-terminal phosphorylation on lHCII conformation in state transition

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Acta Mechanica Sinica 2014年第3期30卷 447-456页

作者： Jin-Hong Ding Ning li Man-liu wang yan zhang Shou-Qin lü mian long Key laboratory of Microgravity(National Microgravity laboratory)and Center of Biomechanics and Bioengineering Institute of MechanicsChinese Academy of Sciences

State transition is an important protection mechanism of plants for maintaining optimal efficiency through redistributing unbalanced excitation energy between photosystem II （PSII） and photosystem I （PSI）. This process depends on the reversible phosphorylation/dephosphorylation of the major light-harvesting complex II （lHCII） and its bi-directional migration between PSII and PSI. But it remains unclear how phosphorylation/dephosphorylation modulates the lHCII conformation and further regulates its reversible migration. Here molecular dynamics simulations （MDS） were employed to elucidate the impact of phosphorylation on lHCII conformation. The results indicated that N-terminal phosphorylation loosened lHCII trimer with decreased hydrogen bond （H-bond） interactions and extended the distances between neighboring monomers, which stemmed from the conformational ad- justment of each monomer itself. Global conformational change of lHCII monomer started from its stromal N- terminal （including the phosphorylation sites） by enhancing its interaction to lipid membrane and by adjusting the interaction network with surrounded inter-monomer andintra-monomer transmembrane helixes of B, C, and A, and finally triggered the reorientation of transmembrane helixes and transferred the conformational change to luminal side helixes and loops. These results further our understanding in molecular mechanism of lHCII migration during state transition from the phosphorylation-induced microstructural feature of lHCII.

关键词： State transition lHCII Phosphorylation Conformation

Global mapping of live cell mechanical features using Peak Force QNM AFM

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Biophysics Reports 2020年第1期6卷 9-18页

作者： Jinrong Hu Shenbao Chen Dandan Huang yan zhang shouqin lü mian long Center of Biomechanics and Bioengineering Key Laboratory of Microgravity (National Microgravity Laboratory)Beijing Key Laboratory of Engineered Construction and Mechanobiology and CAS Center for Excellence in Complex System Mechanics Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Atomic force microscopy(AFM) has been widely used to acquire surface topography upon different scanning modes and to quantify mechanical properties of a cell using single-point ramp force ***, these traditional measurements need massive force curves originating from multiple points of a cell to exclude the potential errors resulted from limited and factitious selections of testing points,making the measurements time-consuming and highly localized. Peak Force Quantitative Nano Mechanics(PF QNM) is a high-speed(faster by 3–4 order of magnitude) and global surface mechanical property mapping method with high spatial resolution, overcoming the drawbacks of traditional ramp mode especially used for a live cell with high heterogeneity. In this protocol, we elaborated how to run PF QNM measurements for live cells and relevant modification may be needed when extending this method to other cell-like soft materials.

关键词： Global mechanical mapping live cell Atomic force microscopy PeakForce QNM