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区域协调性均衡发展理论及我国实践

甘肃社会科学 2020年第4期 188-195页

作者：孟越男徐长乐华东师范大学城市与区域科学学院上海200241 南通大学江苏长江经济带研究院江苏南通226019

以“胡焕庸线”为切入点,分析了我国区域发展的现状。并从概念、任务、特征、内在关系等方面进一步阐述了“协调性均衡发展”这一新概念,进而构建了一个内涵既包括“协调性”又涵盖“均衡性”的新的分析视角。其中,协调性侧重于区域内... 详细信息

以“胡焕庸线”为切入点,分析了我国区域发展的现状。并从概念、任务、特征、内在关系等方面进一步阐述了“协调性均衡发展”这一新概念,进而构建了一个内涵既包括“协调性”又涵盖“均衡性”的新的分析视角。其中,协调性侧重于区域内外的关系和联系,均衡性则重在强调区域众多要素的发展及分布状态,两者既可以在水平与结构、体量与质量、内在与外在、静态与动态、等级与次序等不同视域下独自展开,又可以在“融合共生”的耦合机制下达到辩证统一,形成“一体两面”的统一理论分析框架。从生态、经济和机制三个方面对长三角区域协调性均衡发展进行了论述,并总结出相关结论。

关键词：区域协调性均衡发展一体两面融合共生长三角一体化

CRISPR-powered optothermal nanotweezers:Diverse bio-nanoparticle manipulation and single nucleotide identification

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Light(Science & Applications) 2023年第12期12卷 2644-2654页

作者： Jiajie Chen Zhi Chen Changle Meng Jianxing Zhou Yuhang Peng Xiaoqi Dai Jingfeng Li Yili Zhong Xiaolin Chen Wu Yuan Ho-Pui Ho Bruce Zhi Gao Junle Qu Xueji Zhang Han Zhang Yonghong Shao State Key Laboratory of Radio Frequency Heterogeneous Integration Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China Department of Biomedical Engineering The Chinese University of Hong KongShatinHong KongChina Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA School of Biomedical Engineering Shenzhen UniversityShenzhenGuangdong 518060China

Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of nanoparticles.However,the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles,its potential for identifying bio-nanoparticles remains largely untapped.Hence,based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection,we developed CRISPR-powered optothermal nanotweezers(CRONT).Specifically,by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation,we successfully trapped and enriched DNA functionalized gold nanoparticles,CRISPR-associated proteins,as well as DNA strands.Remarkably,we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism(SNP)detection at single molecule level,while also introducing a novel CRISPR methodology for observing nucleotide cleavage.Therefore,this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable before.With its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification,CRONT will become a universal tool in point-of-care diagnosis,biophotonics,and bio-nanotechnology.

关键词： manipulation thermal identification

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

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National Science Review 2022年第8期9卷 100-109页

作者： Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar A.Al-Hartomy Swelm Wageh Abdullah G.Al-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials Science (RCAMS) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (Shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education 3. Shenzhen Institute of Translational Medicine 4. Department of Otolaryngology Shenzhen Second People’s Hospital 5. the First Affiliated Hospital 6. Institute of Microscale Optoelectronics Shenzhen University 7. Shenzhen International Institute for Biomedical Research 8. Shenzhen Han’s Tech Limited Company 9. Shenzhen Metasensing Tech Limited Company

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive’.Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance(SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat(CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

关键词： surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant

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大容量直驱风电机组级联直流组网系统设计

电机与控制应用 2017年第5期44卷 46-51,79页

作者：厉孟谢桢李长乐海军驻北京作战系统军事代表室北京100094 海军工程大学舰船综合电力技术国防科技重点实验室湖北武汉430033 上海电器科学研究所(集团)有限公司上海200063

传统交流组网风电场系统存在多次电能转换、成本高的问题。针对这个问题,设计了一种大容量直驱风电机组级联直流组网海上风电场系统,其直接将每台机组的直流输出级联形成高压直流进行传输,而无需额外的海上升压站平台。风电机组采用了... 详细信息

传统交流组网风电场系统存在多次电能转换、成本高的问题。针对这个问题,设计了一种大容量直驱风电机组级联直流组网海上风电场系统,其直接将每台机组的直流输出级联形成高压直流进行传输,而无需额外的海上升压站平台。风电机组采用了永磁直驱风力发电机及其变流器,其中变流器包括了AC/DC单元和DC/DC单元,并设计了控制策略,即通过DC/DC单元的占空比调节来实现电流的持续输出和最大功率跟踪。陆基逆变电站采用晶闸管型逆变器,设计了工作模式和控制策略,其主要功能是实现高压直流链路的电压电流调节。最后,基于PSCAD/EMTDC仿真平台,搭建了容量为150 MW的风电场系统进行了仿真计算,计算结果验证了该系统具有较高的鲁棒性和对风速变化的适应性,同时每个机组都能独立的实现最大风能捕获。

关键词：风力发电海上风电场高压直流链路直流组网

Effect of rotating magnetic field on the solidification microstructures of Pb-Sn alloys

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Science China(Technological Sciences) 2006年第3期49卷 274-282页

作者： meng Xiaohua CHEN changle HONG Zhenyu WANG Jianyuan Department of Applied Physics Northwestern Polytechnical University Xi'an 710072 China Department of Applied Physics Northwestern Polytechnical University Xi'an 710072 China Department of Applied Physics Northwestern Polytechnical University Xi'an 710072 China Department of Applied Physics Northwestern Polytechnical University Xi'an 710072 China

The influence of the rotating magnetic field (RMF) on the solidification proc- ess of Pb-Sn binary alloys is studied by comparing the solidification microstructures under the common condition and RMF condition. It is found that the RMF can completely elimi- nate the gravity induced macrosegregation, and result in dendrite fragmentation and promote solute diffusion velocity. These differences are regarded as the effect of compli- cated melt flow caused by RMF. Moreover, when the content of the primary phase is small, many spherical microstructures form under the RMF condition. The analyses indicate that these special microstructures are likely the conjunction action of melt flow and concentra- tion and temperature field uniformity caused by RMF.

关键词： rotating magnetic field, macrosegregation, dendrite fragmentation, melt flow.

新型牵引-制动型液力变矩器液压系统动态性能仿真研究

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机床与液压 2007年第9期35卷 197-200页

作者：孟红项昌乐中国兵器工业集团系统总体部北京100089 北京理工大学机械与车辆工程学院北京100081

基于对某新型牵引-制动型液力变矩减速器的结构和特性分析,建立了其电控液压系统的AMESim仿真模型。通过仿真,研究了其在闭锁过程和制动过程压力动态变化性能。仿真研究表明,特殊设计的液压系统实现了良好的缓冲闭锁过程。

关键词：液力变矩器动态性能仿真

Probucol Prevents Atrial Remodeling by Inhibit ROS Production and NF-κB activation in Alloxan-induced Diabetic Rabbits

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Probucol Prevents Atrial Remodeling by Inhibit ROS Productio...

新型牵引-制动型液力变矩器控制系统的开发设计

中华医学会第十五次全国心血管病学大会

作者： Huaying Fu Tong Liu changle Liu Jian Li Guangping Li Jian Li Lijun Cheng meng Yuan Tianjin Institute of Cardiology Tianjin The second hospital of Tianjin medical university

Objectives this study sought to assess the effects of probucol on atrial remodeling and atrial fibrillation (af) promotion in alloxan-induced diabetic rabbits and to elucidate the underlying mechanisms. Background Diabetes mellitus (dm) is an independent risk factor for af. However, the underlying mechanisms for the increased propensity for af in dm and the effects of probucol on atrial remodeling remain unclear.

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新技术新工艺 2007年第7期 66-69页

作者：孟红项昌乐中国兵器工业集团系统总体部北京100089 北京理工大学机械与车辆工程学院北京100081

基于对新型牵引-制动型液力变矩减速器结构和特性的分析,为了改善其闭锁品质,设计了液力变矩器的闭环反馈控制系统,并通过试验,证明该系统可以输出稳定的PWM波和与之相对应的控制电流变量。

关键词：液力变矩器闭锁控制

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旋转磁场作用下Pb-Sn合金的凝固行为

材料导报 2006年第3期20卷 152-154页

作者：王建元陈长乐孟小华西安西北工业大学理学院应用物理系 710072

采用永磁体旋转装置实现了Pb-50wt%Sn亚共晶、Pb-61.9wt%Sn共晶合金旋转磁场条件下的凝固,并对其凝固规律进行了研究。发现与常规条件下相比,旋转磁场导致的合金熔体内部强迫对流加快了合金熔体的冷却速率,提高熔体初始形核温度,促发熔... 详细信息

采用永磁体旋转装置实现了Pb-50wt%Sn亚共晶、Pb-61.9wt%Sn共晶合金旋转磁场条件下的凝固,并对其凝固规律进行了研究。发现与常规条件下相比,旋转磁场导致的合金熔体内部强迫对流加快了合金熔体的冷却速率,提高熔体初始形核温度,促发熔体提前形核并能使凝固时间缩短。Pb-61.9wt%Sn共晶合金的宏观偏析被有效抑制,在整个样品中得到了二元共晶组织,共晶层片粗化,局域地区出现了不规则共晶组织。对于Pb-50wt%Sn亚共晶,初生相(Pb)的生长方式由粗大枝晶转变为细小的球状颗粒,且晶粒显著细化。

关键词：旋转磁场 Pb—Sn合金形核与生长强迫对流