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Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain

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Journal of Oceanology and Limnology 2024年第4期42卷 1336-1347页

作者： Gao GAO Xing LI Kangxiang QIN Yun HU Xiaosong JIANG Chenxi CHE Yuntao LI changkao mu Chunlin WANG Huan WANG School of Marine Science Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China

The farming of Scylla paramamosain with specific flavors has a higher commercial value,and the flavors are related to the integrated farming environment and non-volatile flavor substances,while the survival environment is one of the important ways to source gut microorganisms in the *** this study,the levels of dominant taxa in the gut flora of *** from Mong Cai,Vietnam(VN),Taishan City,Guangdong Province(TS)of China,and Ninghai County,Ningbo City(NB)Zhejiang Province of China converged with those of *** from Sanmen County,Ningbo City(CK 1,CK 2,and CK 3)at 28 d of *** top 15 genera with the highest abundance of VN,TS,and NB gut flora were the same as CK 1,CK 2,and CK 3,but with different percentages,and gradually converged to CK 1,CK 2,and CK 3,respectively,at 28 d of *** between intestinal flora and non-volatile flavor substances in the hepatopancreas at the percentage level of relative abundance of bacterial genera found that above 28 d of domestication,muribaculaceae,Psychrilyobacter,Clostridia_vadinBB 60_group,Halarcobacter Carboxylicivirga,Sediminispirochaeta may be the most important genera affecting flavor amino acids of ***,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Psychrilyobacter,and Pseudomonas may be the most important genera affecting flavor amino acids of ***,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Vibrio,and Sphingomonas may be the most important genera affecting flavor amino acids of *** results show that the intestinal flora structure of crabs from different areas were domesticated in the same area for at least 28 d before they converged to that of the domesticated crab,and the most important genera affecting the flavor amino acids of TS,VN,and NB were also *** results of this study provide a reference and basis for the technique of directional cultivation of the flavor quality of the crab.

关键词： survival environment Scylla paramamosain intestinal flora structure non-volatile flavor substance directional breeding

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三疣梭子蟹受精卵离体孵化技术

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水产学报 2024年第1期48卷 103-111页

作者：牛雪莹任志明吴佳颖母昌考王春琳宁波大学海洋学院水产生物技术教育部重点实验室宁波大学机械工程与力学学院

为了探究三疣梭子蟹受精卵的离体孵化技术及效果，本研究先后开展了受精卵块最适分离液种类及作用条件的筛选、分离液处理不同发育期受精卵离体孵化的差异、分离液处理对受精卵卵膜的结构影响，及分离液处理受精卵对孵化后幼体活力的影... 详细信息

为了探究三疣梭子蟹受精卵的离体孵化技术及效果，本研究先后开展了受精卵块最适分离液种类及作用条件的筛选、分离液处理不同发育期受精卵离体孵化的差异、分离液处理对受精卵卵膜的结构影响，及分离液处理受精卵对孵化后幼体活力的影响实验。结果显示，木瓜蛋白酶是一种较为理想的分离液，在浓度为0.09 g/mL，分离时间30 min时，分离率可达到99%以上；经过分离液处理后的各期受精卵均能孵化出幼体，卵内溞状幼体期离体胚胎孵化率最高，为89.0%±3.3%，未经处理的对照组为70.0%±4.8%；卵裂期离体胚胎孵化率最低，为58.0%±3.9%，对照组为31.0%±2.3%，与对照组相比，处理组的孵化率明显提高。透射电镜结果显示，经过分离液处理的受精卵卵膜结构疏松，且厚度降低，符合处理组孵化率增加这一现象，干露、福尔马林溶液胁迫和行为学测试对不同处理组的幼体进行质量评价的结果显示，处理组和对照组幼体活力无显著差异。研究表明，实验所获得的分离液可以有效提高三疣梭子蟹受精卵的分离率和孵化率，且不影响幼体质量，可为三疣梭子蟹及其他甲壳动物受精卵的离体孵化提供参考。本研究可为三疣梭子蟹的苗种繁育提供新的技术手段，也将为基因编辑辅助育种等技术的实施奠定基础。

关键词：三疣梭子蟹离体孵化孵化率幼体活力

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Phospholipid Compositions in Portunus trituberculatus Larvae at Different Developmental Stages

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Journal of Ocean University of China 2022年第1期21卷 152-162页

作者： LU Zhibin SHI Ce LIU Lei mu changkao YE Yangfang WANG Chunlin Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo 315211China

Phospholipids are used to improve the growth and survival of Portunus trituberculatus,a widely cultured crab species in ***,only total phospholipids or several classes are applied in crab *** this study,we employed a targeted lipidomic method to investigate the comprehensive phospholipid composition in *** larvae and reveal the changing phospholipid profile over the larval *** showed that *** larvae contain 112 phospholipid species belonging to 10 phospholipid classes,in which phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species are the most abundant,and PC,PE,phosphatidic acid,and phosphatidylserine(PS)are with high *** levels of all phospholipids significantly changed with larval development,which was highlighted by the downward parabolic changes in PE,phosphatidylglycerol,phosphatidylino-sitol,PS,lysophosphatidic acid,and sphingomyelin *** addition,nearly all phospholipid species were depleted at the M stage,which probably contributed to the mass mortality of crab *** findings on the composition and alterations of phospholipids in *** larvae provide novel perspectives for the targeted supplementation of phospholipids in crab *** work also highlights the use of targeted UHPLC-MS lipidomics in understanding the changes of phospholipids during crab development.

关键词： Portunus trituberculatus larval development phospholipid UHPLC-MS

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music stimulus has a positive eff ect on survival and development of the larvae in swimming crab Portunus trituberculatus

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Journal of Oceanology and Limnology 2022年第3期40卷 1277-1285页

作者： Zhiming REN Jiechi WANG Chunlin WANG changkao mu Yangfang YE Ce SHI Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture Ningbo 315211China

The sound stimulus has a signifi cant impact on marine ***,many studies have focused on the negative impacts of sound on marine *** positive impacts of sound on marine animals,especially on marine invertebrates,were rarely *** the present study,we evaluated the eff ects of music on the survival and development of an economically important marine invertebrate,swimming crab Portunus *** pieces of classical music and a control group,i.e.,Eine Kleine Nachtmusik by Mozart(EK),Bach-Violin Concerto No.1(BV),Anonymous Romanza by Anonymous(AR),and environmental sound(EN)were *** sound pressure received were 163-dB root mean square(RMS)re 1μPa for EK,166-dB RMS re 1μPa for BV,165-dB RMS re 1μPa for AR,44-dB RMS re 1μPa for EN,*** larvae were subject to 3-h classical music transmission every day during the entire larval development *** result showed that BV and AR signifi cantly elevated the cumulative survival rate of the larva from 3.42%±1.77%(EN)to 10.65%±1.69%(BV)and 7.12%±1.37%(AR)respectively(Pmusic did not aff ect the cumulative development time of larvae from ZoeaⅠto ZoeaⅣ,it signifi cantly increased the molting rate of megalopa from 24.00%±11.78%(EN)to 44.00%±13.06%(AR)and 46.67%±10.50%(BV)(Pmusical exposure during critical growth intervals may contribute to the survival and development of larval *** an application point of view,music intervention is much economically *** observed music eff ects imply that music could further enhance growth,welfare,and production.

关键词： swimming crab Portunus trituberculatus sound classical music oxygen consumption

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Community composition,co-occurrence,and environmental drivers of bacterioplankton community in surface and 50-m water layers in the subarctic North Pacific

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Journal of Oceanology and Limnology 2023年第6期41卷 2309-2323页

作者： Quandong XIN Jufa CHEN changkao mu Xinliang WANG Wenjing LIU Tao JIANG Yan LI College of Life Sciences Yantai UniversityYantai 264005China Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China School of Marine Sciences Ningbo UniversityNingbo 315211China School of Medicine Yunnan UniversityKunming 650091China

The Western Subarctic Gyre(WSG)is one of the two gyre-systems in the subarctic North Pacific known for high nutrient and low-chlorophyll ***,the bacterioplankton in marine water of this area,either in terms of the taxonomic composition or functional structure,remains relatively unexplored.A total of 22 sampling sites from two water layers(surface water,SW and 50-m layer water,FW)were collected in this *** physiochemical parameters of waters,Synechococcus,and bacterial density,as well as the bacterioplankton community composition and distribution pattern,were *** nutrient concentrations of DIN,DIP,and DSi,Chl-a concentration,and the average abundance of heterobacteria in FW were higher than those in ***,temperature and the average abundance of Synechococcus and pico-eukaryotes were higher in SW.A total of 3269 OTUs were assigned,and 2123OTUs were commonly shared;moreover,similar alpha diversity patterns were observed in both SW and *** bacterioplankton community showed significantly obvious correlation with salinity,DIP,DIN,and Chl a in both SW and ***,Cyanobacteria,Bacteroidota,Actinobacteriota,and Firmicutes were the main phyla while Synechococcus_CC9902,Psychrobacter,and Sulfitobacter were the dominant genera in each sampling *** correlations that happened between the OTUs in the cooccurrence network were positive and *** edges and graph density were found in SW,indicating that more correlations occurred,and the community was more complex in *** study provided novel knowledge on the bacterioplankton community structure and the correlation characteristics in WSG.

关键词： Western Subarctic Gyre(WSG) marine water bacterioplankton community co-occurrence network

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Effect of Long-Term Low-Salinity Culture on the Survival, Growth, and Nutrient Composition of mud Crab Scylla paramamosain

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Journal of Ocean University of China 2022年第1期21卷 179-185页

作者： JI Zilu LI Ronghua WANG Chunlin ZHANG Zhouyi ZHANG Weijia mu changkao SONG Weiwei Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China

This study investigated the effects of long-term low-salinity on the growth performance,proximate composition,amino acid and fatty acid profiles of Scylla *** salinity of the low-salinity-culture group(LC)and maintained-salinity-cul-ture group(MC)were set at 5 and 25 *** rearing for 30 d,the survival rate(SR)of the LC group was significantly lower,whereas the average daily molting frequency(ADMF)was significantly higher than those in MC group(Pmuscle of *** total amino acids(TAA),essen-tial amino acids(EAA)and essential amino acid index(EAAI)had no significant difference between the two *** crabs from LC group had a significantly higher HUFA,including EPA,DHA,*** percentage of n-3 PUFA and the ratio of n-3/n-6 PUFA were significantly higher in the LC *** results indicated that *** rearing at low salinity had significantly de-creased SR;however,the low-salinity environment significantly promoted the accumulation of unsaturated fatty acids(UFAs),espe-cially the n-3 PUFA,which might be related to the osmoregulation mechanism.

关键词： Scylla paramamosain low salinity survival growth nutrient composition

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Protective Effects of Sepia Ink Melanin on Hepatic Tissue in Streptozotocin-Induced Diabetic Mice

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Journal of Ocean University of China 2022年第5期21卷 1362-1372页

作者： WANG Xiaopeng HAN Meng DONG Hui ZHAO Yun SONG Weiwei WANG Chunlin mu changkao LI Ronghua Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315832China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo 315832China

Diabetes mellitus(DM)has emerged as a serious public health concern,due to the high morbidity and mortality resulted from its complications,such as diabetic nephropathy,diabetic cardiovascular complication,and diabetic neuropathy,*** this study,we investigated the beneficial effects of sepia ink melanin(SIM)on hyperglycaemia and the restoration of diabetic symptoms in streptozotocin(STZ)-induced diabetic model *** first,the normal experimental mice were performed with intraperitoneal injection of STZ(40 mg(kg BW)^(−1))(BW,body weight)to attain diabetes and then were treated with different concentrations of SIM(120,240 and 480 mg(kg BW)^(−1))for four *** treatment,significant decrease in gluconeogenesis were determined,accompanied by a notable increase in both glycolysis and oxidative enzyme activities in SIM-treated groups,such as liver marker enzymes in the serum and key antioxidant enzymes in *** results revealed the transcriptional alterations in SIM-treated *** exposure increased the expression levels of several genes related to insulin transduction and PI3K/Akt pathway,including PI3K,Akt,Irs-2,and ***,expression levels of Dicarbonyl/l-xylulose reductase(Dcxr)and UDP-glucose dehydrogenase(Ugdh),which are in-volved in pentose-glucuronate interconversion pathway,were also elevated in SIM-treated ***,histological observation results indicated that nuclear deformation and organelle dissolution were improved,thus could enhance the liver *** results demonstrated that SIM can be effective in ameliorating diabetic symptoms and improving disease management for diabetic patients.

关键词： diabetic hepatopathy sepia ink melanin PI3K/Akt pathway pentose-glucuronate interconversion pathway

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Physiological and Molecular Analyses of Low-Salinity Stress Response in the Cuttlefish(Sepia pharaonis)Juveniles

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Journal of Ocean University of China 2022年第4期21卷 969-976页

作者： XIN Hongwei WU Kunlan YUAN Yimeng ZHAO Yun SONG Weiwei WANG Chunlin mu changkao LI Ronghua Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315832China Collaborative Innovation Center for Zhejiang Marine High-Efficiency Efficiency and Healthy Aquaculture NingboUniversityNingbo 315832China

As a stenohaline species,the survival of Sepia pharaonis can be affected by salinity *** study aimed to explore the function of decreasing salinity on the survival of Sepia pharaonis,which can provide an advanced production guide on the culture of *** in the rainy *** was gradually decreased from 29 to 22 within 48 h to acclimate *** to a low-salinity *** ten days of breeding under low-salinity of 22,the death rate was *** this process,changes in tissue and cell structures in the larval liver,biochemical indicators,and osmoregulation-related gene expression were ***-terestingly,hepatocytes in the low-salinity group were irregular,had dissolved tissue inclusions,and contained vacuolized ***-fore,low salinity caused severe damages at a cellular level that can elevate the mortality rate.A gradual decline in salinity limited the full adaptation of *** indicators and osmoregulation-related gene expression changed *** instance,the trend of malondialdehyde(MAD)as a product of lipid peroxidation reflected the degree of damage to the body by free *** antioxidant system of *** could cope with oxidative stress caused by the change in salinity to a certain ***-regulation-related genes’expression also showed an optimistic result,that is,*** responded positively to the change in sali-nity by adjusting the expression of osmoregulation-related ***,the increase in mortality at day 10 also proved the weak adaptation capability of *** study indicated that *** can adapt to a low-salinity environment with a li-mited extent.

关键词： Sepia pharaonis low salinity physiological responses biochemical indicators osmoregulation-related genes

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Analysis of Key Physiological Characteristics of Portunus trituberculatus in Response to Short-Term Low Salinity Stress

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Journal of Ocean University of China 2023年第3期22卷 746-754页

作者： ZHANG Xinni LIU Qi WANG Jiali GAO Gao NIU Mingming CHEN Lizhi WANG Huan WANG Fangfang WANG Chunlin mu changkao School of Marine Science Ningbo UniversityNingbo 315211China Sanmen Agricultural and Rural Bureau Taizhou 317100China Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China

Salinity is a significant environmental factor that can affect the survival,metamorphosis,growth and feeding of Portunus *** order to analyze the key physiological characteristics of *** in response to short-term low salinity stress,the experiments of gradually decline and recovery as well as abrupt decline in salinity were carried *** results showed that *** could survive in a certain low salinity range in the short term,and salinity 12 was the lowest tolerable salinity under the present experimental *** change of the hemolymph osmotic pressure displayed significant positive correlations with water salinity,and the pressure was always higher than seawater osmotic ***-term low salinity stress changed the structure and morphology of gill *** expansion of gill filament ends and epithelial cell shedding were conducive to osmotic *** activities of key ion transport enzymes such as Na^(+)-K^(+)-ATPase,carbonic anhydrase and V-ATPase also changed with the osmotic regulation,while Na^(+)-K^(+)-ATPase played a dominant *** summary,as an osmotic adjustment species,*** rapidly adapt to the short-term low-salinity environment by osmotic adjustment in vivo,but salinity below salinity 12 is not conducive to its *** result enriched the theoretical mechanism of osmotic regulation of ***,providing reference for the development of aquaculture technology of ***.

关键词： Portunus trituberculatus gradual/abrupt decline in salinity gill osmotic pressure ion transport enzymes

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拟穴青蟹循环水养殖系统生物滤池细菌群落变化

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广东海洋大学学报 2024年第3期44卷 55-63页

作者：周建淑史策叶央芳王春琳母昌考李荣华刘海娟吴清洋王晓朋周月越陈书健宁波大学水产生物技术教育部重点实验室/宁波大学农业农村部绿色海水养殖重点实验室(省部共建) 浙江宁波315832 广西海洋研究所有限责任公司广西北海536000

【目的】探究拟穴青蟹(Scylla paramamosain)循环水养殖系统(Recirculating aquaculture systems,RAS)生物滤池启动过程中细菌群落的演替过程,为设计适合拟穴青蟹RAS养殖的生物过滤器提供指导。【方法】在2、5、10、20、30、40、50 d检... 详细信息

【目的】探究拟穴青蟹(Scylla paramamosain)循环水养殖系统(Recirculating aquaculture systems,RAS)生物滤池启动过程中细菌群落的演替过程,为设计适合拟穴青蟹RAS养殖的生物过滤器提供指导。【方法】在2、5、10、20、30、40、50 d检测RAS养殖池的水质,采集填料生物膜和养殖池水体微生物样品,采用多种生物信息学分析方法研究生物膜微生物群落演替过程,并运用实时荧光定量PCR技术检测生物膜碳循环和氮循环功能基因拷贝数。【结果与结论】氨氮(NH4+-N)、分子氨(NH3)和亚硝态氮(NO2--N)的质量浓度总体呈先上升后下降趋势,硝态氮(NO3--N)和总氮(TN)在实验结束时(50 d)积累,总有机碳(TOC)质量浓度在前10 d保持稳定,从10 d至50 d呈先升高后下降趋势,差异显著(P<0.05),且总氮和硝态氮质量浓度对生物膜细菌群落变化的影响最大。生物膜细菌群落的多样性、均匀性和丰富度在10 d达到峰值,然后达到平稳状态。基于FAPROTAX的功能预测发现生物膜大多数功能主要富集在氮循环和有机物降解这两方面,生物膜碳循环和氮循环绝对基因拷贝数随时间增加总体呈升高趋势。根据FEAST分析结果,除10 d以外,前20 d同一时期的水体细菌对生物膜的贡献较高,后30 d主要来源于生物膜本身前期细菌贡献,且贡献率随时间增加呈增长趋势,到后期趋于稳定。结果表明,拟穴青蟹RAS生物滤池启动过程中,生物膜的细菌群落结构随时间增加变化显著,功能主要富集在氮循环和有机物降解这两方面,生物膜细菌来自本身细菌的贡献率也逐渐升高,到后期趋于稳定。

关键词：拟穴青蟹循环水养殖系统生物膜细菌群落水质功能基因

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