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Surgical mortality in patients with malignant obstructive jaundice: a multivariate discriminant analysis

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Hepatobiliary & Pancreatic Diseases International 2003年第3期2卷 435-440页

作者： Xi-Chun Han Jin-Long Li Gang Han the Department of Surgery, Second Hospital, Jilin University, changchun 130041, China Department of Surgery Second Hospital Jilin University Changchun 130041 China. hanchun601@***

OBJECTIVE: To estimate the operative mortality in patients with malignant obstructive jaundice. METHODS: Twelve risk factors were analyzed using multivariate discriminant analysis in 90 patients who had been operated on. RESULTS: Operative mortality was significantly related to the following factors: age, duration of jaundice, packed RBC volume, white blood cell count and concentration of blood urine nitrogen; it was not significantly related to diseases and types of operation. The following formula was obtained: packed RBC volume×0.09954-age×0. 04018-blood urine nitrogen×0. 23693-duration of jaundice× 2. 07388-WBC count×0. 21118+5. 26593. With this formula, an operative mortality of 77. 8% was predicted. CONCLUSION: With a positive value from the formula, the patient should be operated on; otherwise non-operative treatment is advocated.

关键词： malignant obstructive jaundice postoperative mortality multivariate discriminant analysis

Cloning of Mouse Enamel Matrix Serine Proteinase Encoding Mature Protein

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吉林大学学报（医学版） 2003年第6期29卷 705-709页

作者： MU Ya bing, SUN Hong chen, ZHANG Ze bing, OUYANG Jie (Dept. of Pathology, School of Stomatology, Jilin University,changchun 130041,China) Dept. of Pathology School of Stomatology Jilin University Changchun 130041

目的 :克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶 ( EMSP1 )成熟肽编码区基因。方法 :提取出生后 7d昆明种小白鼠切牙、磨牙牙胚总 RNA,逆转录为 c DNA,设计两对特异性引物 ,采用 Touchdown PCR和嵌套 PCR方法 ,扩增出小鼠 EMSP1起始密码... 详细信息

目的 :克隆小鼠牙胚组织中釉基质丝氨酸蛋白酶 ( EMSP1 )成熟肽编码区基因。方法 :提取出生后 7d昆明种小白鼠切牙、磨牙牙胚总 RNA,逆转录为 c DNA,设计两对特异性引物 ,采用 Touchdown PCR和嵌套 PCR方法 ,扩增出小鼠 EMSP1起始密码子至终止密码子基因片段。将目的基因连入载体 p MD- 1 8T,转化入大肠杆菌 JM1 0 9,通过蓝白筛选 ,挑选阳性克隆培养扩增 ,纯化重组质粒进行限制性酶切和核苷酸序列分析鉴定。结果 :限制性酶切图谱和核苷酸序列分析均表明所克隆 c DNA为小鼠 70 0 bp的 EMSP1成熟肽基因编码。结论

关键词：牙胚小鼠丝氨酸内肽酰类逆转录聚合酶链反应聚合酶链反应克隆分子

Clinical evaluation of serum interleukin 10 in patients with acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2003年第1期2卷 135-138页

作者： Xi-Chun Han Ying-Chao Zhang Yu Wang Ming-Ku Jia the Department of Surgery Secoad Hospital, Jilin University, changchun 130041, China Department of Surgery Second Hospital Jilin University Changchun 130041 China. hanchun6001@***

OBJECTIVES: To evaluate the behavior of serum interleukin 10 (IL-10) in patients with acute pancreatitis and to explore the relationship between this cytokine and the severity of the disease. METHODS: In 45 patients with acute pancreatitis, the serum concentrations of IL-10 was determined on days 1, 2, 3, 4, 5 after admission. Twelve healthy subjects were also studied as controls. These subjects were tested using a commercial ELISA kit. The severity of pancreatitis was determined according to APACHE Ⅱ score and Balthazar CT criteria. RESULTS: Healthy subjects had no detectable serum levels of IL-10. In acute pancreatitis patients, the serum IL-10 levels were increased on the first day after the onset of the disease and then progressively decreased in the following days. On the first day after the onset of acute pancreatitis, the serum levels of IL-10 in patients with mild acute pancreatitis were significantly higher than in those with severe acute pancreatitis. In the following days, however, no statistically significant difference was observed between the two groups. CONCLUSIONS: Serum IL-10 concentration reflects the severity of acute pancreatitis. IL-10 is a useful variable for early prediction of the prognosis of acute pancreatitis. The low values of serum IL-10 in patients with severe acute pancreatitis suggests that there may be altered down-regulation of immune system response. An enhanced release of IL-10 may be a method for early treatment of acute pancreatitis.

关键词： interleukin 10 acute pancreatitis

Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein

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Chinese Journal of Traumatology 2005年第3期8卷 132-137页

作者：刘一张绍昆吴宏Department of Ophthamology Second Hospital Jilin University changchun 130041 China 单玉兴王刚徐莘香 Department of Orthopaedic Surgery First Hospital Jilin University Changchun 130021China

Objective: To label human insulin-like growth factor-I （hIGF-I） eukaryotic expression vector with green fluorescent protein （GFP） for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into pcDNA3.1-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. Results: Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of （hIGF-1） and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. Conclusions: hIGF-I eukaryotic expression vector has been successfully labeled with GFP.

关键词： Insulin-like growth factor I Transgenes Green fluorescent protein

Impact of Interleukin-28B Polymorphism on Response to Treatment in Chinese Hepatitis C Patients

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Chemical Research in Chinese Universities 2012年第1期28卷 79-83页

作者： Lü Jian-nan1, WANG Song2, XU Yan1, ZHANG Man-hua1, SIQING Tunala1, JI Shang-wei1, JIAO Jian1, WANG Xu1 and WANG Jiang-bin1 1. China-Japan Union Hospital of Jilin University, changchun 130033, P. R. China 2. The Second Hospital of Jilin University, changchun 130041, P. R. China China-Japan Union Hospital of Jilin University Changchun 130033 The Second Hospital of Jilin University Changchun 130041

The candidate polymorphism rs12979860 of interleukin 28B（IL28B） gene was genotyped by means of polymerase chain reaction（PCR） amplification and direct PCR sequencing, and then the role of this single nucleotide polymorphism（SNP） in the response to the treatment of 172 Chinese patients with hepatitis C virus（HCV） chronic infection was analyzed. The results show that the frequencies of major homozygotes（CC） and heterozygotes（CT） of rs12979860 were 0.84 and 0.16, respectively, and the minor homozygotes（TT） wasn’t found in this cohort. A highly significant association was found between the CC genotype and sustained virological response（SVR） in HCV geno type 1 infected patients（P〈0.001） but not in HCV non-genotype 1 infected patients. In addition, a strong association was found between rs12979860 CC genotype and rapid virological response（RVR） in both HCV genotype 1 infected patients（P〈0.001） and non-genotype 1 infected patients（P〈0.001）. Taken together, these results indicate that IL28B polymorphism plays a key role in response to hepatitis C therapy in Chinese patients. Combine assessment of clinical predictors and the IL28B polymorphism may be beneficial to the individualized treatment decision in Asian chronic hepatitis C（CHC） patients.

关键词： Hepatitis C virus(HCV) Interleukin 28B(IL28B) Interleukin-28B Gene

Calcium Overload Is A Critical Step in Programmed Necrosis of ARPE-19 Cells Induced by High-Concentration H_2O_2

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Biomedical and Environmental Sciences 2010年第5期23卷 371-377页

作者： GUANG-YU LI BIN FAN YONG-CHEN ZHENG Department of Ophthalmology Second Hospital of Jilin University Changchun 130041 Jilin China

Objective Oxidative stress plays an important role in retinal pigmental epithelium （RPE） death during aging and the development of age-related macular *** early reports indicate that reactive oxygen species （ROS） including H2O2 can trigger apoptosis at lower concentrations and necrosis at higher concentrations,the exact molecular mechanism of RPE death is still *** purpose of this study was to investigate the molecular pathways involved in RPE death induced by exogenous ROS,especially at higher *** Cultured ARPE-19 cells were treated with H2O2 at different concentrations and cell viability was measured with the MTT *** death was morphologically studied by microscopy using APOPercentage assay and PI ***,the impact of oxidative stress on ARPE-19 cells was assessed by HO-1 and PARP-1 Western blotting and by the protection of antioxidant *** influx was determined using the fura-2 calcium indicator and the role of intracellular calcium overload in ARPE-19 cell death was evaluated following cobalt treatment to block calcium *** H2O2 reduced the viability of ARPE-19 cells in a concentration-dependent manner,which was presented as a typical s-shaped *** death caused by high concentrations of H2O2 was confirmed to be programmed ***,dying ARPE-19 cells were extremely swollen and lost the integrity of their plasma membrane,positively detected with APOPercentage assay and PI staining.24-hour treatment with 500 ？mol/L H2O2 induced remarkable up-regulation of HO-1 and PARP-1 in ARPE-19 ***,antioxidant treatment using EGCG effectively protected cells from H2O2-induced injury,increasing cell viability from 14.17%±2.31% to 85.77%±4.58%.After H2O2 treatment,intracellular calcium levels were highly elevated with a maximum concentration of ***,the calcium channel inhibitor cobalt was able to blunt this calcium influx and blocked the necrotic

关键词： Apoptosis ARPE-19 cell Necrosis Oxidative-stressed injury Hydrogen peroxide

Effects of adenosine agonist R-phenylisopropyl-adenosine on halothane anesthesia and antinociception in rats

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Acta Pharmacologica Sinica 2005年第2期26卷 181-185页

作者： Hai-chun MA~(2,4),Yan-fen WANG~3,Chun-sheng FENG~2,Hua ZHAO~(4,5),Shuji DOHI~6~2Department of Anesthesiology,the First Hospital of Jilin University,changchun 130021,China ~3Department of Digestive Disease,the SecondHospital of Jilin University,changchun 130041,China ~4Department of Physiology,Basic Medical School,Jilin University,changchun 130021,China ~6Department of Anesthesiology,School of Medicine,Gifu University,Gifu 5008705,Japan Department of Anesthesiology the First Hospital of Jilin University Changchun China Department of Physiology Basic Medical School Jilin University Changchun China Department of Digestive Disease the Second Hospital of Jilin University Changchun China Department of Anesthesiology School of Medicine Gifu University Gifu Japan

Aim:To investigate the antinociceptive effect of adenosine agonist R-phenylisopropyl-adenosine（R-PIA）given to conscious rats by intracerebro-ventricular（ICV）and intrathecal（IT）,and identify the effect of R-PIA on minimumalveolar concentration（MAC）of halothane with pretreatment of A1receptor an-tagonist 8-cyclopentyl-1,3-dipropylxanthine（DPCPX）or K+channel blocker4-aminopyridine（4-AP）.Methods:Sprague-Dawley rats were implanted with 24-gauge stainless steel guide cannula using stereotaxic apparatus and ICV method,and an IT catheter（PE-10,8.5cm）was inserted into the lumbar subarachnoidspace,while the rats were under pentobarbital *** one week ofrecovery from surgery,rats were randomly assigned to one of the followingprotocols:MAC of halothane,or tail-flick *** measurements were per-formed after R-PIA（0.8-2.0μg）microinjection into ICV and IT with or withoutpretreatment of DPCPX or ***:Microinjection of adenosine agonist R-PIA in doses of 0.8-2.0μg into ICV and IT produced a significant dose-and time-dependent antinociceptive action as reflected by increasing latency times andICV administration of adenosine agonist R-PIA（0.8μg）reducing halothane anes-thetic requirements（by 29%）.The antinociception and reducing halothane re-quirements effected by adenosine agonist R-PIA was abolished by DPCPX ***:ICV and IT administration of adenosine agonist R-PIA pro-duced an antinociceptive effect in a dose-dependent manner and decreased hal-othane MAC with painful stimulation through activation of A1receptor subtype,and the underlying mechanism involves K+channel activation.

关键词： adenosine inhalation anesthesia analgesia potassium channels subarachnoid space cerebral ventricles

Expression of intercellular adhesion m olecule-1and H L A-D R antigens in uveitis

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吉林大学学报(医学版) 1999年第4期25卷 393-395页

作者： Li Xu 1,Wang Guiyun 1,Zhang Xiaoguang 1,Sui Guiqin 2 (1 Department of Ophthalmology,The Second Clinical College,Norman Bethune University of Medical Sciences,changchun 130041 2\ Jilin Dispensary of Ophthalmopathy) \ \ 白求恩医科大学第二临床学院吉林

目的：研究细胞间粘附分子１（ｉｎｔｅｌｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１，ＩＣＡＭ１）和人体组织相关抗原（ｈｕｍａｎｌｅｕｋｏｃｙｔｅａｎｔｉｇｅｎ，ＨＬＡＤＲ）在葡萄膜炎免疫反应中的作... 详细信息

目的：研究细胞间粘附分子１（ｉｎｔｅｌｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１，ＩＣＡＭ１）和人体组织相关抗原（ｈｕｍａｎｌｅｕｋｏｃｙｔｅａｎｔｉｇｅｎ，ＨＬＡＤＲ）在葡萄膜炎免疫反应中的作用。方法：应用免疫组织化学染色检查２０只正常眼和５４例葡萄糖膜炎眼球摘除眼（其中外源性３３例和内源性２１例）的脉络膜和视网膜组织中ＩＣＡＭ１和ＨＬＡＤＲ的表达。结果：正常眼的脉络膜和视网膜组织没有ＩＣＡＭ１的阳性染色，没有或较少有ＨＬＡＤＲ的表达，葡萄膜炎眼中二者有增高表达（Ｐ＜００１），而外源性和内源性葡萄膜炎眼组间表达统计学上无显著差异（Ｐ＞００５）。结论：ＩＣＡＭ１、ＨＬＡＤＲ分子能够介导白细胞和炎症部位组织细胞的识别和粘附，二者的共同表达说明它们在葡萄糖膜炎脉络膜视网膜组织的免疫性损伤中具有重要意义。

关键词：细胞间粘附分子1,葡萄膜炎,免疫组织化学,人体组织相关抗原

Ischaemia preconditioning attenuates hypothermic ischaemia reperfusion related early graft dysfunction following orthotopic lung transplantation in the rat

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吉林大学学报(医学版) 2000年第2期26卷 162-165页

作者： Zhang Xingyi 1,Sun Mei 2,Wang Rongyou 1 (Department of Thoracic Surgery 1 and Pathology 2,Second Clinical College of the Bethune University of Medical Sciences,changchun,130041,China) 〔白求恩医科大学附属第二医院吉林长春 130041 白求恩医科大学吉林长春 130041

目的 :为了证明缺血预处理对移植肺功能的影响。方法 :使用套接吻合技术行大鼠左肺同位肺移植 ,大鼠分为两组 1对照组 ( n=1 6) :同位肺移植未行缺血预处理。 2 IP组 ( n=1 5) :同位肺移植前行缺血预处理。结果 :IP组血气变化和受鼠生... 详细信息

目的 :为了证明缺血预处理对移植肺功能的影响。方法 :使用套接吻合技术行大鼠左肺同位肺移植 ,大鼠分为两组 1对照组 ( n=1 6) :同位肺移植未行缺血预处理。 2 IP组 ( n=1 5) :同位肺移植前行缺血预处理。结果 :IP组血气变化和受鼠生存期较对照组有明显改善。血管外水分含量亦较对照组明显减少。结论 :缺血预处理不仅能减少移植肺损伤而且能改善移植肺功能不全。

关键词： ? Orthotopic lung transplantation ischaemia preconditioning